Laboratory Investigation - Table of Contents alert Volume 97 Issue 7

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TABLE OF CONTENTS Volume 97, Issue 7 (July 2017) In this issue Inside the USCAP Journals Research Articles Technical Reports Also new AOP Like us on Facebook Twitter Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement We are pleased to announce that the Impact Factor* for Laboratory Investigation is now 4.857 - an increase from 4.202 last year. Laboratory Investigation is ranked 9 of 79 in the Pathology category and 16 of 128 in the Medicine, Research & Experimental category. The journal would like to thank all our authors, reviewers and readers for their continued support. *2016 Journal Citation Reports (Clarivate Analytics, 2017)  Submit your research >>> Inside the USCAP Journals Top Malignant melanoma: from cause to cure 2017 97: 762-763; 10.1038/labinvest.2017.58 Full Text Research Articles Top BLOOD, LYMPHATICS, IMMUNE SYSTEM AND STEM CELLS Angiotensin-converting enzyme affects the presentation of MHC class II antigens The peptidase angiotensin converting enzyme participates in processing MHC class II peptides, which, in turn, activate T helper cells and facilitate antibody production. Manipulation of the activity of this peptidase by, for example, pharmacological inhibition, may prove to be a novel strategy to alter the immune response. Tuantuan Zhao, Kenneth E Bernstein, Jianmin Fang and Xiao Z Shen 2017 97: 764-771; advance online publication, April 10, 2017; 10.1038/labinvest.2017.32 Abstract | Full Text Disruption of direct 3D telomere–TRF2 interaction through two molecularly disparate mechanisms is a hallmark of primary Hodgkin and Reed–Sternberg cells Disruption of direct 3D telomere- telomere repeat binding factor 2 (TRF2) interaction is a hallmark of primary Hodgkin and Reed-Sternberg cells. Two different molecular mechanisms appear to be involved: the first, attrition of telomeres and up-regulation of TRF2 protein that is not associated with teleomeres; and the second, with telomere deprotection due to an impressive loss of TRF2 that is physically linked to telomeres. This second pattern is typical of LMP1+ EBV-associated classical Hodgkin lymphoma but is not restricted to it. Hans Knecht, Nathalie A Johnson, Tina Haliotis, Daniel Lichtensztejn and Sabine Mai 2017 97: 772-781; advance online publication, April 24, 2017; 10.1038/labinvest.2017.33 Abstract | Full Text ENDOCRINE, VISUAL AND AUDITORY SYSTEMS Cytochrome P450 (CYP) epoxygenases as potential targets in the management of impaired diabetic wound healing This paper shows that reduced expression of cytochrome P450 epoxygenases may play a role in the pathogenesis of impaired diabetic wound healing. Exogenous epoxyeicosatrienoic acid administration significantly improves wound healing in ob/ob mice, possibly through attenuation of inflammatory responses and promotion of angiogenesis. Huichen Zhao, Jicui Chen, Jiachao Chai, Yuchao Zhang, Cong Yu, Zhe Pan, Peng Gao, Chen Zong, Qingbo Guan, Yuqin Fu and Yuantao Liu 2017 97: 782-791; advance online publication, March 20, 2017; 10.1038/labinvest.2017.21 Abstract | Full Text Advanced oxidation protein products promote NADPH oxidase-dependent β-cell destruction and dysfunction through the Bcl-2/Bax apoptotic pathway This study reveals new targets for the prevention and treatment of β-cell destruction seen in diverse disorders such as diabetes, chronic kidney disease, obesity and metabolic syndrome. The authors show that accumulation of advanced oxidation protein products triggers β-cell apoptosis and results in deficiency of insulin secretion through NADPH oxidase-dependent O−2 generation via Bcl-2 and Bax. Min Liang, Aiqing Li, Aiju Lou, Xifang Zhang, Youyuan Chen, Lei Yang, Yumin Li, Shenglin Yang and Fan Fan Hou 2017 97: 792-805; advance online publication, March 6, 2017; 10.1038/labinvest.2017.24 Abstract | Full Text DEVELOPMENT, MATRIX BIOLOGY AND AGING Endothelial cell-specific activation of transforming growth factor-β signaling in mice induces cutaneous, visceral, and microvascular fibrosis To examine microvascular alterations in systemic sclerosis (SSc), novel transgenic mice with inducible endothelial cell-specific constitutively active TGF-β receptor I expression were generated. The mice displayed severe cutaneous and visceral fibrosis and marked perivascular and subendothelialfibrosis, mimicking SSc characteristic features. Increased fibrosis and myofibroblast activation gene expression were demonstrated. Peter J Wermuth, Kellan R Carney, Fabian A Mendoza, Sonsoles Piera-Velazquez and Sergio A Jimenez 2017 97: 806-818; advance online publication, March 27, 2017; 10.1038/labinvest.2017.23 Abstract | Full Text HEPATIC AND PANCREATIC SYSTEMS Hedgehog signaling pathway affects the sensitivity of hepatoma cells to drug therapy through the ABCC1 transporter This study employed a newly characterized CD133−/EpCAM− subpopulation (Huh-7-DN) that features high hedgehog signaling activity and epithelial-mesenchymal transition to investigate how hedgehog signaling controls chemosensitivity by an ATP-binding complex molecule, ABCC1. The findings demonstrate that transcription factor Gli-2 governs the target gene ABCC1 that modulates drug resistance in this subpopulation. Jia Ding, Xiao-Tian Zhou, Hao-Yu Zou and Jian Wu 2017 97: 819-832; advance online publication, April 17, 2017; 10.1038/labinvest.2017.34 Abstract | Full Text Establishment of a mouse model of enalapril-induced liver injury and investigation of the pathogenesis A mouse model of enalapril-induced liver injury was established by co-treatment with dexamethasone and L-buthionine-(S,R)-sulfoximine, which cause hepatic steatosis and glutathione depletion, respectively. The authors found that oxidative stress and neutrophil infiltration are involved in the pathogenesis of enalapril-induced liver injury. Yuji Shirai, Shingo Oda, Sayaka Makino, Koichi Tsuneyama and Tsuyoshi Yokoi 2017 97: 833-842; advance online publication, March 6, 2017; 10.1038/labinvest.2017.22 Abstract | Full Text The Hippo signaling functions through the Notch signaling to regulate intrahepatic bile duct development in mammals Using conditional knockout mice, the authors found that the excessive bile duct development induced by deficiency of the Hippo signaling pathway tumor suppressor Nf2 is significantly suppressed by heterozygous and homozygous deletion of Notch2 in a dose-dependent manner. These results suggest that the Notch signaling pathway is a downstream effector of the Hippo signaling pathway in regulating intrahepatic bile duct development. Nan Wu, Quy Nguyen, Ying Wan, Tiaohao Zhou, Julie Venter, Gabriel A Frampton, Sharon DeMorrow, Duojia Pan, Fanyin Meng, Shannon Glaser, Gianfranco Alpini and Haibo Bai 2017 97: 843-853; advance online publication, June 5, 2017; 10.1038/labinvest.2017.29 Abstract | Full Text Technical Reports Top MODELS AND TECHNIQUES Increased vasohibin-1 expression is associated with metastasis and poor prognosis of renal cell carcinoma patients Vasohibin-1 (VASH-1), a regulator of angiogenesis, is predominantly expressed in endothelial cells within clear cell renal cell carcinoma (ccRCC) tissue, whereas it is not expressed in non-neoplastic renal tissues. Elevated VASH-1 density is associated with tumor progression and sunitinib treatment. These data suggest that VASH1 may be a prognostic biomarker of ccRCC. Shuji Mikami, Mototsugu Oya, Takeo Kosaka, Ryuichi Mizuno, Yasumasa Miyazaki, Yasufumi Sato and Yasunori Okada 2017 97: 854-862; advance online publication, March 13, 2017; 10.1038/labinvest.2017.26 Abstract | Full Text Optimized expression-based microdissection of formalin-fixed lung cancer tissue This study provides a workflow for expression-based microdissection adapted for lung adenocarcinoma. The authors improved microdissection by optimizing immunostaining intensity, processing of the stained samples and by providing instructions for laser handling. Using this procedure, the authors could enrich mutated EGFR-DNA from lung adenocarcinoma tissue samples. Selectivity and efficiency of microdissection was further demonstrated by pre-post whole-slide image analysis. Markus Grafen, Thurid R Hofmann, Andreas H Scheel, Julia Beck, Alexander Emmert, Stefan Küffer, Bernhard C Danner, Ekkehard Schütz, Reinhardt Büttner, Andreas Ostendorf, Philipp Ströbel and Hanibal Bohnenberger 2017 97: 863-872; advance online publication, April 24, 2017; 10.1038/labinvest.2017.31 Abstract | Full Text Fully automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification and same species antibodies The authors developed a primary antibody/secondary antibody complex heat-deactivation method which enables fully automated fluorescent multiplex immunohistochemistry using unmodified primary antibodies from same species on formalin-fixed, paraffin embedded tissue. This approach allows evaluation of spatial relationships and the functional states of multiple biomarkers on a single slide, and offers great flexibility for research and possibility for in vitro diagnostic product development. Wenjun Zhang, Antony Hubbard, Tobin Jones, Adriana Racolta, Srabani Bhaumik, Nick Cummins, Liping Zhang, Karl Garsha, Frank Ventura, Mark R Lefever, Zhenqiang Lu, John K Hurley, William A Day, Lidija Pestic-Dragovich, Larry E Morrison and Lei Tang 2017 97: 873-885; advance online publication, May 15, 2017; 10.1038/labinvest.2017.37 Abstract | Full Text Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department. 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