Nikon's A1rMP+ multiphoton system is now available with a New Dual IR beam option. With dual IR beams, users can now simultaneously excite two different fluorophores such as GFP and mCherry. This capability enables ultra-fast two-color multiphoton imaging, ideal for dynamic specimens.
EEG and functional ultrasound imaging in mobile rats pp831 - 834 Lim-Anna Sieu, Antoine Bergel, Elodie Tiran, Thomas Deffieux, Mathieu Pernot et al. doi:10.1038/nmeth.3506 Functional ultrasound imaging and electroencephalography are combined to assess brain activity in mobile rats. The methodology is applied to the analysis of theta rhythms in a maze task and of epileptic seizures.
Efficient and quantitative high-throughput tRNA sequencing pp835 - 837 Guanqun Zheng, Yidan Qin, Wesley C Clark, Qing Dai, Chengqi Yi et al. doi:10.1038/nmeth.3478 The combination of an engineered demthylase and a highly processive reverse transcriptase during tRNA library preparation for high-throughput sequencing allows comprehensive profiling of the small RNAs.
Inferring transient particle transport dynamics in live cells pp838 - 840 Nilah Monnier, Zachary Barry, Hye Yoon Park, Kuan-Chung Su, Zachary Katz et al. doi:10.1038/nmeth.3483 Single-particle dynamics are analyzed with hidden Markov modeling in combination with Bayesian model selection. This method can annotate both diffusive and directed motion states with single-step resolution.
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