Nature Cell Biology contents: March 2017 Volume 19 Number 3, pp 145 - 258

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Advertisement ATCC® CRISPR/Cas9 Gene-edited Isogenic Cell Lines  Isogenic cell lines are engineered from tumor cell lines relevant to diseases and drug targets and possess key mutations, such as EML4-ALK fusion and NRAS Q61K. ATCC isogenic cell lines undergo a thorough molecular validation at the genomic, transcript, and protein levels. We perform additional bio-functional characterization to demonstrate drug-screening applications.  Start your drug screening studies with the new isogenic cell line! www.atcc.org/isogenic TABLE OF CONTENTS March 2017 Volume 19, Issue 3 Editorial News and Views Articles Letter Erratum Advertisement Nikon is offering special pricing on our award winning Eclipse Ts2 inverted tissue culture microscope with phase contrast optics. The Ts2 features intuitive controls, a compact body, LED illumination, and smart accessories such as the Contrast Shield which allows fluorescent samples to be easily observed in brightly lit labs. For more information, go to  www.nikoninstruments.com/ts2-promo Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement Bioprocessing Technologies in Stem Cell Research: Challenges and Chances for Commercialization The 1st Stem Cell Community Day brings together experts from industry and academia to discuss recent achievements, challenges, and chances in stem cell bioprocessing for research and commercial manufacturing. Register now and submit your abstract!  www.stemcellday.de/   Advertisement ELECTRON MICROSCOPY FOR MATERIALS - THE NEXT TEN YEARS Presented by: Zhejiang University | Nature | Nature Materials The conference will feature sessions on nanomaterials, functional materials, structural material, soft matter and biomaterials, and techniques development. May 27-29, 2016 | Zhenjiang, China  Register now!   Editorial Top The scientist citizen: time to become political   p145 doi:10.1038/ncb3489 Shifting political landscapes in the United States and European Union have seeded uncertainty in the scientific community. Intellectual freedom, funding and scientist mobility must be protected to secure the future of biological research. News and Views Top Pulling cells out of tumours   pp147 - 149 Andrew J. Ewald doi:10.1038/ncb3484 Tumours are highly complex and contain multiple cell types. Cancer-associated fibroblasts are now shown to have a critical role in directly leading cancer cell invasion. This intercellular interaction relies on a mechanically active cadherin-based junction, and CAF-led invasion is demonstrated to require E-cadherin in the cancer cell. See also: Article by Labernadie et al. G1 cyclins protect pluripotency   pp149 - 150 Julia Arand and Julien Sage doi:10.1038/ncb3480 G1 cyclins are considered essential for DNA replication and cell division. A recent report now shows that some cells can cycle in the absence of G1 cyclins. In embryonic stem cells and cancer cells, G1 cyclins are required to activate cyclin-dependent kinases to phosphorylate core pluripotency factors and maintain pluripotency. See also: Article by Liu et al. Inheritance of protection from osmotic stress   pp151 - 152 Kiyomi R. Kaneshiro and Susan Strome doi:10.1038/ncb3483 Exposure of mother worms to mild osmotic stress induces gene expression changes in offspring that protect them from strong osmotic stress. Inheritance of protection is now shown to depend on altered insulin-like signalling in the maternal germline, which confers protection through increased expression of zygotic gpdh-2, a rate-limiting enzyme in glycerol biosynthesis. See also: Letter by Burton et al. Twist of fate for skeletal muscle mesenchymal cells   pp153 - 154 Natalya A. Goloviznina and Michael Kyba doi:10.1038/ncb3482 Skeletal muscles are composed of different types of fibres. Can these be thought of as distinct lineages with specific lineage-restricted progenitors? A provocative study now proposes that mesenchymal cells expressing the transcription factor Twist2 act as myogenic progenitors with selective type IIb fibre-differentiation potential. See also: Article by Liu et al. Nature Cell Biology JOBS of the week Postdoctoral position: RNA, Stem Cell and Cancer Biology Lund University Postdoctoral fellow position in the area of cancer cell biology University Hospital Cologne Postdoctoral Fellow in Stem Cell Biology and Pharmacogenomics Northwestern University Feinberg School of Medicine, Chicago 2 PhD Positions in Theoretical Cell Biology Heinrich Heine University, Düsseldorf PhD Graduate program in Immunobiology, Epigenetics and Cell Biology at IMPRS-MCB Max Planck Institute of Immunobiology and Epigenetics More Science jobs from Nature Cell Biology EVENT Plant Cell and Development Biology 22.05.17 Suzhou, China More science events from Advertisement Thousands of single cells. One solution.  From the industry leaders in sequencing and Droplet Digital™ technologies, Illumina and Bio-Rad provide a robust, scalable, and user-friendly workflow enabling transcriptome profiling of hundreds to tens of thousands of single cells.   Articles Top Tissue-scale coordination of cellular behaviour promotes epidermal wound repair in live mice   pp155 - 163 Sangbum Park, David G. Gonzalez, Boris Guirao, Jonathan D. Boucher, Katie Cockburn et al. doi:10.1038/ncb3472 Park et al. study individual cell dynamics during mouse wound re-epithelialization in real time and reveal a finely orchestrated interplay between epidermal migration, directional division and differentiation. Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive   pp164 - 176 Nai Yang Fu, Anne C. Rios, Bhupinder Pal, Charity W. Law, Paul Jamieson et al. doi:10.1038/ncb3471 Fu et al. define a heterogeneous population of MaSC subsets based on the expression of LGR5 and TSPAN8, with varying anatomical locations in the mouse mammary ductal tree, in vivo repopulating abilities, cell cycle status and molecular signatures. G1 cyclins link proliferation, pluripotency and differentiation of embryonic stem cells   pp177 - 188 Lijun Liu, Wojciech Michowski, Hiroyuki Inuzuka, Kouhei Shimizu, Naoe Taira Nihira et al. doi:10.1038/ncb3474 Liu et al. show that G1 cyclins and their cyclin-dependent kinases regulate the pluripotent state by driving phosphorylation of Nanog, Oct4 and Sox2, thereby identifying a direct connection between G1 cyclins and pluripotency factors. See also: News and Views by Arand & Sage Cell–matrix signals specify bone endothelial cells during developmental osteogenesis   pp189 - 201 Urs H. Langen, Mara E. Pitulescu, Jung Mo Kim, Rocio Enriquez-Gasca, Kishor K. Sivaraj et al. doi:10.1038/ncb3476 Langen et al. identify a third capillary endothelial cell subtype, termed type E, that supports embryonic and early postnatal bone formation, and show that endothelial integrin β1 and laminin α5 are required for bone angiogenesis and osteogenesis. A Twist2-dependent progenitor cell contributes to adult skeletal muscle   pp202 - 213 Ning Liu, Glynnis A. Garry, Stephen Li, Svetlana Bezprozvannaya, Efrain Sanchez-Ortiz et al. doi:10.1038/ncb3477 Liu et al. identify an interstitial progenitor cell, characterized by Twist2 expression, that is highly myogenic, forms type IIb/x myofibres and contributes to regeneration in adult skeletal muscle. See also: News and Views by Goloviznina & Kyba Differential cytokine contributions of perivascular haematopoietic stem cell niches   pp214 - 223 Noboru Asada, Yuya Kunisaki, Halley Pierce, Zichen Wang, Nicolas F. Fernandez et al. doi:10.1038/ncb3475 Asada et al. examine differential effects of CXCL12 and SCF expression by perivascular bone marrow niche cells, such as arteriolar NG2+ vascular smooth muscle cells and sinusoidal LepR+ cells, on haematopoietic stem cell maintenance and mobilization. A mechanically active heterotypic E–cadherin/N–cadherin adhesion enables fibroblasts to drive cancer cell invasion   pp224 - 237 Anna Labernadie, Takuya Kato, Agustí Brugués, Xavier Serra-Picamal, Stefanie Derzsi et al. doi:10.1038/ncb3478 Cancer-associated fibroblasts (CAFs) promote metastasis by creating tracks for cancer cell migration. Labernadie et al. now show that heterotypic adhesions between E-cadherin on cancer cells and N-cadherin on CAFs transmit forces to drive invasion. See also: News and Views by Ewald The LINK-A lncRNA interacts with PtdIns(3,4,5)P3 to hyperactivate AKT and confer resistance to AKT inhibitors   pp238 - 251 Aifu Lin, Qingsong Hu, Chunlai Li, Zhen Xing, Guolin Ma et al. doi:10.1038/ncb3473 Yang and colleagues report that the LINK-A lncRNA binds to the PIP3 phospholipid, leading to enhanced AKT signalling, tumorigenesis and resistance to AKT inhibitors. Letter Top Insulin-like signalling to the maternal germline controls progeny response to osmotic stress   pp252 - 257 Nicholas O. Burton, Tokiko Furuta, Amy K. Webster, Rebecca E. W. Kaplan, L. Ryan Baugh et al. doi:10.1038/ncb3470 Burton et al. show that Caenorhabditis elegans parental exposure to osmotic stress protects progeny from osmotic stress through insulin-like signalling, linking maternal germline signalling to progeny metabolism. See also: News and Views by Kaneshiro & Strome Erratum Top Erratum: Long-range self-organization of cytoskeletal myosin II filament stacks   p258 Shiqiong Hu, Kinjal Dasbiswas, Zhenhuan Guo, Yee-Han Tee, Visalatchi Thiagarajan et al. doi:10.1038/ncb3479 Top Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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