Scientific Reports Biology Table of Contents e-alert: 14 February 2017

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14 February 2017      • Visit Scientific Reports • Submit your manuscript to Scientific Reports     Welcome to your Table of Contents e-alert for Scientific Reports       Biological Sciences               High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells ▶     Shengtai Bian, Yicen Zhou, Yawei Hu et al.     Arrayed genetic screens mediated by the CRISPR/Cas9 technology with single guide RNA (sgRNA) libraries demand a high-throughput platform capable of transfecting diverse cell …             Metabolomic tool to identify soybean [Glycine max (L.) Merrill] germplasms with a high level of shade tolerance at the seedling stage ▶     Jiang Liu, Baoyu Hu, Weiguo Liu et al.     The isoflavone profiles of seeds of various soybean genotypes with different levels of shade tolerance at the seedling stage were investigated. High-performance liquid …             Opposing effects of reward and punishment on human vigor ▶     Benjamin Griffiths, Ulrik R. Beierholm     The vigor with which humans and animals engage in a task is often a determinant of the likelihood of the task’s success. An influential theoretical model suggests that the speed …             Luman contributes to brefeldin A-induced prion protein gene expression by interacting with the ERSE26 element ▶     Marc-André Déry, Andréa C. LeBlanc     The cellular prion protein (PrP) is essential for transmissible prion diseases, but its exact physiological function remains unclear. Better understanding the regulation of the …             A ‘selfish’ B chromosome induces genome elimination by disrupting the histone code in the jewel wasp Nasonia vitripennis ▶     John C. Aldrich, Alexandra Leibholz, Manjinder S. Cheema et al.     Intragenomic conflict describes a phenomenon in which genetic elements act ‘selfishly’ to gain a transmission advantage at the expense of the whole genome. A non-essential, …             CuII(atsm) improves the neurological phenotype and survival of SOD1G93A mice and selectively increases enzymatically active SOD1 in the spinal cord ▶     James B. Hilton, Stephen W. Mercer, Nastasia K. H. Lim et al.     Ubiquitous expression of mutant Cu/Zn-superoxide dismutase (SOD1) selectively affects motor neurons in the central nervous system (CNS), causing the adult-onset degenerative …             An end-to-end software solution for the analysis of high-throughput single-cell migration data ▶     Paola Masuzzo, Lynn Huyck, Aleksandra Simiczyjew et al.     The systematic study of single-cell migration requires the availability of software for assisting data inspection, quality control and analysis. This is especially important for …             Concentration-dependent oligomerization of an alpha-helical antifreeze polypeptide makes it hyperactive ▶     Sheikh Mahatabuddin, Yuichi Hanada, Yoshiyuki Nishimiya et al.     A supersoluble 40-residue type I antifreeze protein (AFP) was discovered in a righteye flounder, the barfin plaice (bp). Unlike all other AFPs characterized to date, bpAFP …             Dynamics of male meiotic recombination frequency during plant development using Fluorescent Tagged Lines in Arabidopsis thaliana ▶     Fan Li, Nico De Storme, Danny Geelen     Meiotic homologous recombination plays a central role in creating genetic variability, making it an essential biological process relevant to evolution and crop breeding. In this …             Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors ▶     Sravan Kumar Goparaju, Kazuhisa Kohda, Keiji Ibata et al.     Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative …             Profound seasonal shrinking and regrowth of the ossified braincase in phylogenetically distant mammals with similar life histories ▶     Dina K. N. Dechmann, Scott LaPoint, Christian Dullin et al.     Ontogenetic changes in skull shape and size are ubiquitous in altricial vertebrates, but typically unidirectional and minimal in full-grown animals. Red-toothed shrews exhibit a …             Eco-evolutionary feedbacks can rescue cooperation in microbial populations ▶     Clara Moreno-Fenoll, Matteo Cavaliere, Esteban Martínez-García et al.     Bacterial populations whose growth depends on the cooperative production of public goods are usually threatened by the rise of cheaters that do not contribute but just consume …             Subthalamic Neurons Encode Both Single- and Multi-Limb Movements in Parkinson’s Disease Patients ▶     Ariel Tankus, Ido Strauss, Tanya Gurevich et al.     The subthalamic nucleus (STN) is the main target for neurosurgical treatment of motor signs of Parkinson’s disease (PD). Despite the therapeutic effect on both upper and lower …             Corrigendum: In vivo dynamics of active edema and lethal factors during anthrax ▶     Clémence Rougeaux, François Becher, Eric Ezan et al.                 Extracellular protonation modulates cell-cell interaction mechanics and tissue invasion in human melanoma cells ▶     Verena Hofschröer, Kevin Alexander Koch, Florian Timo Ludwig et al.     Detachment of cells from the primary tumour precedes metastatic progression by facilitating cell release into the tissue. Solid tumours exhibit altered pH homeostasis with …             Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging ▶     Shigenori Inagaki, Hidekazu Tsutsui, Kazushi Suzuki et al.     We report development of the first genetically encoded bioluminescent indicator for membrane voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require …             Bladder cancer cell growth and motility implicate cannabinoid 2 receptor-mediated modifications of sphingolipids metabolism ▶     Arianna Bettiga, Massimo Aureli, Giorgia Colciago et al.     The inhibitory effects demonstrated by activation of cannabinoid receptors (CB) on cancer proliferation and migration may also play critical roles in controlling bladder cancer …             Adaptability of small brown planthopper to four rice cultivars using life table and population projection method ▶     Xiao-Min Zheng, Yun-Li Tao, Hsin Chi et al.     In this study, we evaluated the adaptability of the small brown planthopper (SBPH), Laodelphax striatellus (Hemiptera: Delphacidae) to four rice cultivars including Shengdao13 …             The microbe-secreted isopeptide poly-γ-glutamic acid induces stress tolerance in Brassica napus L. seedlings by activating crosstalk between H2O2 and Ca2+ ▶     Peng Lei, Xiao Pang, Xiaohai Feng et al.     Poly-γ-glutamic acid (γ-PGA) is a microbe-secreted isopeptide that has been shown to promote growth and enhance stress tolerance in crops. However, its site of action and …             Increased anterior cingulate cortex response precedes behavioural adaptation in anorexia nervosa ▶     Daniel Geisler, Franziska Ritschel, Joseph A. King et al.     Patients with anorexia nervosa (AN) are characterised by increased self-control, cognitive rigidity and impairments in set-shifting, but the underlying neural mechanisms are …             Identification of Extracellular Segments by Mass Spectrometry Improves Topology Prediction of Transmembrane Proteins ▶     Tamás Langó, Gergely Róna, Éva Hunyadi-Gulyás et al.     Transmembrane proteins play crucial role in signaling, ion transport, nutrient uptake, as well as in maintaining the dynamic equilibrium between the internal and external …             Anaerobic Capacityestimated in A Single Supramaximal Test in Cycling: Validity and Reliability Analysis ▶     Willian Eiji Miyagi, Rodrigo de Araujo Bonetti de Poli, Marcelo Papoti et al.     The aim was to verify the validity (i.e., study A) and reliability (i.e., study B) of the alternative maximal accumulated oxygen deficit determined using onlya supramaximal …             Applying phasor approach analysis of multiphoton FLIM measurements to probe the metabolic activity of three-dimensional in vitro cell culture models ▶     Pirmin H. Lakner, Michael G. Monaghan, Yvonne Möller et al.     Fluorescence lifetime imaging microscopy (FLIM) can measure and discriminate endogenous fluorophores present in biological samples. This study seeks to identify FLIM as a …             Activating Killer Immunoglobulin Receptors and HLA-C: a successful combination providing HIV-1 control ▶     Mauro S. Malnati, Elisabetta Ugolotti, Maria Cristina Monti et al.     Several studies demonstrated a relevant role of polymorphisms located within the HLA-B and -C loci and the Killer Immunoglobulin Receptors (KIRs) 3DL1 and 3DS1 in controlling …             Responses of spatial-temporal dynamics of bacterioplankton community to large-scale reservoir operation: a case study in the Three Gorges Reservoir, China ▶     Zhe Li, Lunhui Lu, Jinsong Guo et al.     Large rivers are commonly regulated by damming, yet the effects of such disruption on bacterioplankton community structures have not been adequately studied. The aim of this …             AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells ▶     Yuko Ueda, Ikumi Ooshio, Yasuyuki Fusamae et al.     The mammalian AlkB homolog (ALKBH) family of proteins possess a 2-oxoglutarate- and Fe(II)-dependent oxygenase domain. A similar domain in the Escherichia coli AlkB protein …             Inhibitory effects of cholinesterase inhibitor donepezil on the Kv1.5 potassium channel ▶     Kai Li, Neng Cheng, Xian-Tao Li     Kv1.5 channels carry ultra-rapid delayed rectifier K+ currents in excitable cells, including neurons and cardiac myocytes. In the current study, the effects of cholinesterase …             Proteins analysed as virtual knots ▶     Keith Alexander, Alexander J. Taylor, Mark R. Dennis     Long, flexible physical filaments are naturally tangled and knotted, from macroscopic string down to long-chain molecules. The existence of knotting in a filament naturally …             β-Thalassemia Patients Revealed a Significant Change of Untargeted Metabolites in Comparison to Healthy Individuals ▶     Syed Ghulam Musharraf, Ayesha Iqbal, Saqib Hussain Ansari et al.     β-Thalassemia is one of the most prevalent forms of congenital blood disorders characterized by reduced hemoglobin levels with severe complications, affecting all dimensions of …             The N-terminal domain of Schmallenberg virus envelope protein Gc is highly immunogenic and can provide protection from infection ▶     Kerstin Wernike, Andrea Aebischer, Gleyder Roman-Sosa et al.     Schmallenberg virus (SBV) is transmitted by insect vectors, and therefore vaccination is one of the most important tools of disease control. In our study, novel subunit vaccines …             Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease ▶     Sachiko Furukawa, Masafumi Moriyama, Kensuke Miyake et al.     IgG4-related disease (IgG4-RD) is characterized by elevated serum IgG4 and marked infiltration of IgG4-positive cells in multiple organs. Interleukin-33 (IL-33) is a recently …             Heteroaryldihydropyrimidine (HAP) and Sulfamoylbenzamide (SBA) Inhibit Hepatitis B Virus Replication by Different Molecular Mechanisms ▶     Zheng Zhou, Taishan Hu, Xue Zhou et al.     Heteroaryldihydropyrimidine (HAP) and sulfamoylbenzamide (SBA) are promising non-nucleos(t)ide HBV replication inhibitors. HAPs are known to promote core protein mis-assembly, …             System-wide detection of protein-small molecule complexes suggests extensive metabolite regulation in plants ▶     Daniel Veyel, Sylwia Kierszniowska, Monika Kosmacz et al.     Protein small molecule interactions are at the core of cell regulation controlling metabolism and development. We reasoned that due to the lack of system wide approaches only a …             Antifouling potential of Nature-inspired sulfated compounds ▶     Joana R. Almeida, Marta Correia-da-Silva, Emília Sousa et al.     Natural products with a sulfated scaffold have emerged as antifouling agents with low or nontoxic effects to the environment. In this study 13 sulfated polyphenols were …             Structural and functional characterization of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease ▶     Likui Zhang, Dandan Xu, Yanchao Huang et al.     HNH endonucleases in bacteriophages play a variety of roles in the phage lifecycle as key components of phage DNA packaging machines. The deep-sea thermophilic bacteriophage …             Plant-based vaccines for oral delivery of type 1 diabetes-related autoantigens: Evaluating oral tolerance mechanisms and disease prevention in NOD mice ▶     Amanda L. Posgai, Clive H. Wasserfall, Kwang-Chul Kwon et al.     Autoantigen-specific immunological tolerance represents a central objective for prevention of type 1 diabetes (T1D). Previous studies demonstrated mucosal antigen administration …             Electroacupuncture Attenuates CFA-induced Inflammatory Pain by suppressing Nav1.8 through S100B, TRPV1, Opioid, and Adenosine Pathways in Mice ▶     Hsien-Yin Liao, Ching-Liang Hsieh, Chun-Ping Huang et al.     Pain is associated with several conditions, such as inflammation, that result from altered peripheral nerve properties. Electroacupuncture (EA) is a common Chinese clinical …             Erratum: Risk of prenatal depression and stress treatment: alteration on serotonin system of offspring through exposure to Fluoxetine ▶     Siran Pei, Li Liu, Zhaomin Zhong et al.                 Dynamic properties of calcium-activated chloride currents in Xenopus laevis oocytes ▶     Ildefonso M. De la Fuente, Iker Malaina, Alberto Pérez-Samartín et al.     Chloride is the most abundant permeable anion in the cell, and numerous studies in the last two decades highlight the great importance and broad physiological role of chloride …             Paenibacillus polymyxa NSY50 suppresses Fusarium wilt in cucumbers by regulating the rhizospheric microbial community ▶     Lu Shi, Nanshan Du, Sheng Shu et al.     Paenibacillus polymyxa (P. polymyxa) NSY50, isolated from vinegar residue substrate, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host …             Entorhinal Cortex dysfunction can be rescued by inhibition of microglial RAGE in an Alzheimer’s disease mouse model ▶     Chiara Criscuolo, Veronica Fontebasso, Silvia Middei et al.     The Entorhinal cortex (EC) has been implicated in the early stages of Alzheimer’s disease (AD). In particular, spreading of neuronal dysfunction within the EC-Hippocampal network …             Reduced aldehyde dehydrogenase expression in preeclamptic decidual mesenchymal stem/stromal cells is restored by aldehyde dehydrogenase agonists ▶     Gina D. Kusuma, Mohamed H. Abumaree, Anthony V. Perkins et al.     High resistance to oxidative stress is a common feature of mesenchymal stem/stromal cells (MSC) and is associated with higher cell survival and ability to respond to oxidative …             Interferon-α-inducible Dendritic Cells Matured with OK-432 Exhibit TRAIL and Fas Ligand Pathway-mediated Killer Activity ▶     Terutsugu Koya, Ryu Yanagisawa, Yumiko Higuchi et al.     Active human dendritic cells (DCs), which efficiently induce immune responses through their functions as antigen-presenting cells, exhibit direct anti-tumour killing activity in …             Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons ▶     Wen-Shin Chen, Yi-Ju Chen, Yung-An Huang et al.     The microtubule (MT) cytoskeleton is essential for the formation of morphologically appropriate neurons. The existence of the acentrosomal MT organizing center in neurons has …             Connexin30.3 is expressed in mouse embryonic stem cells and is responsive to leukemia inhibitory factor ▶     Mikako Saito, Yuma Asai, Keiichi Imai et al.     The expression of 19 connexin (Cx) isoforms was observed in the mouse embryonic stem (ES) cell line, EB3. Their expression patterns could be classified into either pluripotent …             HRGFish: A database of hypoxia responsive genes in fishes ▶     Iliyas Rashid, Naresh Sahebrao Nagpure, Prachi Srivastava et al.     Several studies have highlighted the changes in the gene expression due to the hypoxia response in fishes, but the systematic organization of the information and the analytical …             Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis ▶     Javier Sotillo, Ivana Ferreira, Jeremy Potriquet et al.     Different reports have highlighted the potential use of helminths and their secretions in the treatment of inflammatory bowel disease (IBD) conditions; however, no reports have …             Amyloid-β Oligomers Interact with Neurexin and Diminish Neurexin-mediated Excitatory Presynaptic Organization ▶     Yusuke Naito, Yuko Tanabe, Alfred Kihoon Lee et al.     Alzheimer’s disease (AD) is characterized by excessive production and deposition of amyloid-beta (Aβ) proteins as well as synapse dysfunction and loss. While soluble Aβ oligomers …             Comparison of biological H2S removal characteristics between a composite packing material with and without functional microorganisms ▶     Rencheng Zhu, Shunyi Li, Xiaofeng Bao et al.     The performances of two identical biofilters, filled with a new composite packing material (named CM-5) embedded with functional microorganisms or sterilized CM-5 without …             The inflammatory state provokes sexual dimorphism in left ventricular and electrocardiographic effects of chronic cyclosporine in rats ▶     Hany M. El-Bassossy, Zainy M. Banjar, Mahmoud M. El-Mas     Although cardiotoxicity has been recognized as an adverse effect of cyclosporine A (CSA), no information exists regarding sex specificity of CSA cardiotoxicity. We tested the …             Experimental Study of the Biological Properties of Human Embryonic Stem Cell–Derived Retinal Progenitor Cells ▶     Jingzhi Shao, Peng-Yi Zhou, Guang-Hua Peng     Retinal degenerative diseases are among the leading causes of blindness worldwide, and cell replacement is considered as a promising therapeutic. However, the resources of seed …             Arrays of Individual DNA Molecules on Nanopatterned Substrates ▶     Roland Hager, Alma Halilovic, Jonathan R. Burns et al.     Arrays of individual molecules can combine the advantages of microarrays and single-molecule studies. They miniaturize assays to reduce sample and reagent consumption and …             Link between plasminogen activator inhibitor-1 and cardiovascular risk in chronic hepatitis C after viral clearance ▶     Ming-Ling Chang, Yu-sheng Lin, Li-Heng Pao et al.     The pathophysiological implications of plasminogen activator inhibitor-1 (PAI-1) in HCV infection remain obscure. This prospective study evaluated 669 HCV patients, of whom 536 …             Association evidence of CCTTT repeat polymorphism in the iNOS promoter and the risk of atrial fibrillation in Taiwanese ▶     Lung-An Hsu, Yung-Hsin Yeh, Wei-Jan Chen et al.     Inducible nitric oxide synthase (iNOS) plays an important role in the pathogenesis of atrial fibrillation (AF). The iNOS promoter has a CCTTT-repeat length polymorphism that can …             Spatial regularity of InAs-GaAs quantum dots: quantifying the dependence of lateral ordering on growth rate ▶     T. Konishi, E. Clarke, C. W. Burrows et al.     The lateral ordering of arrays of self-assembled InAs-GaAs quantum dots (QDs) has been quantified as a function of growth rate, using the Hopkins-Skellam index (HSI). Coherent QD …             Predicting antimicrobial peptides with improved accuracy by incorporating the compositional, physico-chemical and structural features into Chou’s general PseAAC ▶     Prabina Kumar Meher, Tanmaya Kumar Sahu, Varsha Saini et al.     Antimicrobial peptides (AMPs) are important components of the innate immune system that have been found to be effective against disease causing pathogens. Identification of AMPs …             Flavichalasines A–M, cytochalasan alkaloids from Aspergillus flavipes ▶     Guangzheng Wei, Dongdong Tan, Chunmei Chen et al.     Two new tetracyclic cytochalasans, flavichalasines A and B (1 and 2), three new pentacyclic cytochalasans, flavichalasines C–E (3–5), and eight new tricyclic cytochalasans, …             Vascular smooth muscle cell glycocalyx mediates shear stress-induced contractile responses via a Rho kinase (ROCK)-myosin light chain phosphatase (MLCP) pathway ▶     Hongyan Kang, Jiajia Liu, Anqiang Sun et al.     The vascular smooth muscle cells (VSMCs) are exposed to interstitial flow induced shear stress that may be sensed by the surface glycocalyx, a surface layer composed primarily of …             Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability ▶     Junko Ueki, Masayuki Nakamori, Masahiro Nakamura et al.     Myotonic dystrophy type 1 (DM1) is an autosomal-dominant multi-system disease caused by expanded CTG repeats in dystrophia myotonica protein kinase (DMPK). The expanded CTG …             Linking gene expression to productivity to unravel long- and short-term responses of seagrasses exposed to CO2 in volcanic vents ▶     Irene Olivé, João Silva, Chiara Lauritano et al.     Ocean acidification is a major threat for marine life but seagrasses are expected to benefit from high CO2. In situ (long-term) and transplanted (short-term) plant incubations of …             Testicular activin and follistatin levels are elevated during the course of experimental autoimmune epididymo–orchitis in mice ▶     Nour Nicolas, Vera Michel, Sudhanshu Bhushan et al.     Experimental autoimmune epididymo-orchitis (EAEO) is a model of chronic inflammation, induced by immunisation with testicular antigens, which reproduces the pathology of some …             Crucial Role of Mesangial Cell-derived Connective Tissue Growth Factor in a Mouse Model of Anti-Glomerular Basement Membrane Glomerulonephritis ▶     Naohiro Toda, Kiyoshi Mori, Masato Kasahara et al.     Connective tissue growth factor (CTGF) coordinates the signaling of growth factors and promotes fibrosis. Neonatal death of systemic CTGF knockout (KO) mice has hampered analysis …             Acetate fluxes in Escherichia coli are determined by the thermodynamic control of the Pta-AckA pathway ▶     Brice Enjalbert, Pierre Millard, Mickael Dinclaux et al.     Escherichia coli excretes acetate upon growth on fermentable sugars, but the regulation of this production remains elusive. Acetate excretion on excess glucose is thought to be …             Optimal designs of mollusk shells from bivalves to snails ▶     Takuya Okabe, Jin Yoshimura     Bivalve, ammonite and snail shells are described by a small number of geometrical parameters. Raup noted that the vast majority of theoretically possible shell forms do not occur …             Corrigendum: Lipid-II Independent Antimicrobial Mechanism of Nisin Depends On Its Crowding And Degree Of Oligomerization ▶     Ashutosh Prince, Padmani Sandhu, Pankaj Ror et al.                 3-bromopyruvate ameliorate autoimmune arthritis by modulating Th17/Treg cell differentiation and suppressing dendritic cell activation ▶     Takaichi Okano, Jun Saegusa, Keisuke Nishimura et al.     Recent studies have shown that cellular metabolism plays an important role in regulating immune cell functions. In immune cell differentiation, both interleukin-17-producing T …             The combination of the functionalities of feedback circuits is determinant for the attractors’ number and size in pathway-like Boolean networks ▶     Eugenio Azpeitia, Stalin Muñoz, Daniel González-Tokman et al.     Molecular regulation was initially assumed to follow both a unidirectional and a hierarchical organization forming pathways. Regulatory processes, however, form highly …             Visualizing dynamics of angiogenic sprouting from a three-dimensional microvasculature model using stage-top optical coherence tomography ▶     Haruko Takahashi, Keisuke Kato, Kenji Ueyama et al.     Three-dimensional (3D) in vitro microvasculature in a polydimethylsiloxane-based microdevice was developed as a physiologically relevant model of angiogenesis. The angiogenic …             Genome-wide identification of the TIFY gene family in three cultivated Gossypium species and the expression of JAZ genes ▶     Quan Sun, Guanghao Wang, Xiao Zhang et al.     TIFY proteins are plant-specific proteins containing TIFY, JAZ, PPD and ZML subfamilies. A total of 50, 54 and 28 members of the TIFY gene family in three cultivated cotton …             Identification of crude drugs in the Japanese pharmacopoeia using a DNA barcoding system ▶     Xiaochen Chen, Li Xiang, Linchun Shi et al.     Kampo is the general designation for traditional Japanese herbal medicines, which are recognized as official medicines and listed in the Japanese pharmacopoeia (JP). In most …             Corrigendum: Neuronal basis of perceptual learning in striate cortex ▶     Zhen Ren, Jiawei Zhou, Zhimo Yao et al.                 Transcriptome analysis reveals a complex interplay between resistance and effector genes during the compatible lentil-Colletotrichum lentis interaction ▶     Vijai Bhadauria, Perumal Vijayan, Yangdou Wei et al.     Colletotrichum lentis is a hemibiotrophic pathogen and causes anthracnose on lentil. To understand the molecular mechanism underlying the symptomatic phase of infection, a cDNA …             GlcNAc6ST-1 regulates sulfation of N-glycans and myelination in the peripheral nervous system ▶     Takeshi Yoshimura, Akiko Hayashi, Mai Handa-Narumi et al.     Highly specialized glial cells wrap axons with a multilayered myelin membrane in vertebrates. Myelin serves essential roles in the functioning of the nervous system. Axonal …             Netrin-1 Improves Functional Recovery through Autophagy Regulation by Activating the AMPK/mTOR Signaling Pathway in Rats with Spinal Cord Injury ▶     Liangjie Bai, Xifan Mei, Zhaoliang Shen et al.     Autophagy is an process for the degradation of cytoplasmic aggregated proteins and damaged organelles and plays an important role in the development of SCI. In this study, we …             Stable Cretaceous sex chromosomes enable molecular sexing in softshell turtles (Testudines: Trionychidae) ▶     Michail Rovatsos, Peter Praschag, Uwe Fritz et al.     Turtles demonstrate variability in sex determination ranging from environmental sex determination (ESD) to highly differentiated sex chromosomes. However, the evolutionary …             Speed versus endurance tradeoff in plants: Leaves with higher photosynthetic rates show stronger seasonal declines ▶     Yong-Jiang Zhang, Lawren Sack, Kun-Fang Cao et al.     We tested for a tradeoff across species between plant maximum photosynthetic rate and the ability to maintain photosynthesis under adverse conditions in the unfavorable season. …             Functionalized Graphene Oxide with Chitosan for Protein Nanocarriers to Protect against Enzymatic Cleavage and Retain Collagenase Activity ▶     Fatemeh Emadi, Abbas Amini, Ahmad Gholami et al.     Proteins have short half-life because of enzymatic cleavage. Here, a new protein nanocarrier made of graphene oxide (GO) + Chitosan (CS) is proposed to successfully prevent …             Superresolution imaging of nanoscale chromosome contacts ▶     Yejun Wang, Prasuna Ratna, G. V. Shivashankar     Co-expression of a specific group of genes requires physical associations among these genes, which form functional chromosomal contacts. While DNA fluorescence in situ …             Stability, structural and functional properties of a monomeric, calcium–loaded adenylate cyclase toxin, CyaA, from Bordetella pertussis ▶     Sara E. Cannella, Véronique Yvette Ntsogo Enguéné, Marilyne Davi et al.     Bordetella pertussis, the causative agent of whooping cough, secretes an adenylate cyclase toxin, CyaA, which invades eukaryotic cells and alters their physiology by cAMP …             Dimerisation induced formation of the active site and the identification of three metal sites in EAL-phosphodiesterases ▶     Dom Bellini, Sam Horrell, Andrew Hutchin et al.     The bacterial second messenger cyclic di-3′,5′-guanosine monophosphate (c-di-GMP) is a key regulator of bacterial motility and virulence. As high levels of c-di-GMP are …             Ultrasensitive Label-free Electrochemical Immunosensor based on Multifunctionalized Graphene Nanocomposites for the Detection of Alpha Fetoprotein ▶     Yaoguang Wang, Yong Zhang, Dan Wu et al.     In this work, a novel label-free electrochemical immunosensor was developed for the quantitative detection of alpha fetoprotein (AFP). Multifunctionalized graphene nanocomposites …             Activated niacin receptor HCA2 inhibits chemoattractant-mediated macrophage migration via Gβγ/PKC/ERK1/2 pathway and heterologous receptor desensitization ▶     Ying Shi, Xiangru Lai, Lingyan Ye et al.     The niacin receptor HCA2 is implicated in controlling inflammatory host responses with yet poorly understood mechanistic basis. We previously reported that HCA2 in A431 …             FOXO1-suppressed miR-424 regulates the proliferation and osteogenic differentiation of MSCs by targeting FGF2 under oxidative stress ▶     Liangping Li, Qihua Qi, Jiaquan Luo et al.     Recently, microRNAs (miRNAs) have been identified as key regulators of the proliferation and differentiation of mesenchymal stem cells (MSCs). Our previous in vivo study and …             A new approach to quantification of mAb aggregates using peptide affinity probes ▶     Crystal S. F. Cheung, Kyle W. Anderson, Pooja M. Patel et al.     Using mAbs as therapeutic molecules is complicated by the propensity of mAbs to aggregate at elevated concentrations, which can lead to a variety of adverse events in treatment. …             Dual Immunomagnetic Nanobeads-Based Lateral Flow Test Strip for Simultaneous Quantitative Detection of Carcinoembryonic Antigen and Neuron Specific Enolase ▶     Wenting Lu, Kan Wang, Kun Xiao et al.     A novel immunomagnetic nanobeads -based lateral flow test strip was developed for the simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic …             A neural signature of the unique hues ▶     Lewis Forder, Jenny Bosten, Xun He et al.     Since at least the 17th century there has been the idea that there are four simple and perceptually pure “unique” hues: red, yellow, green, and blue, and that all other hues are …             New natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18 ▶     Constanze Paulus, Yuriy Rebets, Bogdan Tokovenko et al.     Marine actinobacteria are drawing more and more attention as a promising source of new natural products. Here we report isolation, genome sequencing and metabolic profiling of …             Temporal and spatial instability in neutral and adaptive (MHC) genetic variation in marginal salmon populations ▶     Kate Ciborowski, William C Jordan, Carlos Garcia de Leaniz et al.     The role of marginal populations for the long-term maintenance of species’ genetic diversity and evolutionary potential is particularly timely in view of the range shifts caused …             Identification of Symmetrical RNA Editing Events in the Mitochondria of Salvia miltiorrhiza by Strand-specific RNA Sequencing ▶     Bin Wu, Haimei Chen, Junjie Shao et al.     Salvia miltiorrhiza is one of the most widely-used medicinal plants. Here, we systematically analyzed the RNA editing events in its mitochondria. We developed a pipeline using …             Virulence Inhibitors from Brazilian Peppertree Block Quorum Sensing and Abate Dermonecrosis in Skin Infection Models ▶     Amelia Muhs, James T. Lyles, Corey P. Parlet et al.     Widespread antibiotic resistance is on the rise and current therapies are becoming increasingly limited in both scope and efficacy. Methicillin-resistant Staphylococcus aureus …             Preclinical evaluation of bacterially produced RSV-G protein vaccine: Strong protection against RSV challenge in cotton rat model ▶     Sandra Fuentes, Laura Klenow, Hana Golding et al.     In current study, we evaluated the safety and protective efficacy of recombinant unglycosylated RSV G protein ectodomain produced in E. coli (in presence and absence of …             A novel ECG analog 4-(S)-(2,4,6-trimethylthiobenzyl)-epigallocatechin gallate selectively induces apoptosis of B16-F10 melanoma via activation of autophagy and ROS ▶     Jing Xie, Ju-ping Yun, Ya-nan Yang et al.     Autophagy-induced cancer cell death has become a novel strategy for the development of cancer therapeutic drugs. Numerous studies have indicated that green tea polyphenols induce …             Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions ▶     Vered Israeli-Ruimy, Pedro Bule, Sadanari Jindou et al.     Protein-protein interactions play a vital role in cellular processes as exemplified by assembly of the intricate multi-enzyme cellulosome complex. Cellulosomes are assembled by …             Ubiquitination mediates Kv1.3 endocytosis as a mechanism for protein kinase C-dependent modulation ▶     Ramón Martínez-Mármol, Katarzyna Styrczewska, Mireia Pérez-Verdaguer et al.     The voltage-dependent potassium channel Kv1.3 plays essential physiological functions in the immune system. Kv1.3, regulating the membrane potential, facilitates downstream Ca2+ …             Targeting of phage particles towards endothelial cells by antibodies selected through a multi-parameter selection strategy ▶     Ole A. Mandrup, Simon Lykkemark, Peter Kristensen     One of the hallmarks of cancer is sustained angiogenesis. Here, normal endothelial cells are activated, and their formation of new blood vessels leads to continued tumour growth. …             Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina ▶     Gerrit Hilgen, Sahar Pirmoradian, Daniela Pamplona et al.     We have investigated the ontogeny of light-driven responses in mouse retinal ganglion cells (RGCs). Using a large-scale, high-density multielectrode array, we recorded from …             CENP-A chromatin disassembly in stressed and senescent murine cells ▶     Sabrine Hédouin, Giacomo Grillo, Ivana Ivkovic et al.     Centromeres are chromosomal domains essential for genomic stability. We report here the remarkable transcriptional and epigenetic perturbations at murine centromeres in genotoxic …             Responses of the cutworm Spodoptera litura (Lepidoptera: Noctuidae) to two Bt corn hybrids expressing Cry1Ab ▶     Shu Yinghua, Du Yan, Chen Jin et al.     To examine the responses of the secondary lepidopteran pest Spodoptera litura to two Bacillus thuringiensis (Bt) corn hybrids [5422Bt1 (Event Bt11), 5422CBCL (MON810)] expressing …             The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures ▶     Ilnam Kang, Suhyun Kim, Md. Rashedul Islam et al.     The acI lineage of the phylum Actinobacteria is the most abundant bacterial group in most freshwater lakes. However, due to difficulties in laboratory cultivation, only two mixed …             Three-dimensional collagen matrix induces a mechanosensitive invasive epithelial phenotype ▶     Shawn P. Carey, Karen E. Martin, Cynthia A. Reinhart-King     A critical step in breast cancer progression is local tissue invasion, during which cells pass from the epithelial compartment to the stromal compartment. We recently showed that …             Engineering the residual side chains of HAP phytases to improve their pepsin resistance and catalytic efficiency ▶     Canfang Niu, Peilong Yang, Huiying Luo et al.     Strong resistance to proteolytic attack is important for feed enzymes. Here, we selected three predicted pepsin cleavage sites, L99, L162, and E230 (numbering from the initiator …             Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy ▶     Suguru Ohira, Hideo Tanaka, Yoshinori Harada et al.     Raman spectroscopy, which identifies intrinsic molecular constituents, has a potential for determining myocardial viability under label-free conditions. However, its suitability …             Impact of Dehydroepiandrosterone Sulfate on Newborn Leukocyte Telomere Length ▶     Han Liu, Guangdi Zhou, Qian Chen et al.     The newborn setting of leukocyte telomere length (LTL) likely has important implications for telomere dynamics over the lifespan. However, its determinants are poorly understood. …             Reduced Necrosis and Content of Apoptotic M1 Macrophages in Advanced Atherosclerotic Plaques of Mice With Macrophage-Specific Loss of Trpc3 ▶     Sumeet Solanki, Prabhatchandra R. Dube, Lutz Birnbaumer et al.     In previous work we reported that ApoeKO mice transplanted with bone marrow cells deficient in the Transient Receptor Potential Canonical 3 (TRPC3) channel have reduced necrosis …             Corrigendum: Quality of Life and Cost-Effectiveness of Radiofrequency Ablation versus Open Surgery for Benign Thyroid Nodules: a retrospective cohort study ▶     Wen-Wen Yue, Shu-Rong Wang, Feng Lu et al.                 GLCCI1 rs37973: a potential genetic predictor of therapeutic response to inhaled corticosteroids in Chinese chronic obstructive pulmonary disease patients ▶     Yuan Lei, Yiping Gao, Jinkun Chen et al.     Inhaled corticosteroids (ICSs) are widely prescribed in chronic obstructive pulmonary disease (COPD). However, little is known about predictors of ICSs therapeutic response. To …             Detailed Vascular Anatomy of the Human Retina by Projection-Resolved Optical Coherence Tomography Angiography ▶     J. P. Campbell, M. Zhang, T. S. Hwang et al.     Optical coherence tomography angiography (OCTA) is a noninvasive method of 3D imaging of the retinal and choroidal circulations. However, vascular depth discrimination is limited …             The spectral and spatial distribution of light pollution in the waters of the northern Gulf of Aqaba (Eilat) ▶     Raz Tamir, Amit Lerner, Carynelisa Haspel et al.     The urbanization of the shores of the Gulf of Aqaba has exposed the marine environment there, including unique fringing coral reefs, to strong anthropogenic light sources. Here …             Unbiased K-mer Analysis Reveals Changes in Copy Number of Highly Repetitive Sequences During Maize Domestication and Improvement ▶     Sanzhen Liu, Jun Zheng, Pierre Migeon et al.     The major component of complex genomes is repetitive elements, which remain recalcitrant to characterization. Using maize as a model system, we analyzed whole genome shotgun …             Association of polygenic risk for major psychiatric illness with subcortical volumes and white matter integrity in UK Biobank ▶     L. M. Reus, X. Shen, J. Gibson et al.     Major depressive disorder (MDD), schizophrenia (SCZ) and bipolar disorder (BP) are common, disabling and heritable psychiatric diseases with a complex overlapping polygenic …             Topological determinants of self-sustained activity in a simple model of excitable dynamics on graphs ▶     Christoph Fretter, Annick Lesne, Claus C. Hilgetag et al.     Simple models of excitable dynamics on graphs are an efficient framework for studying the interplay between network topology and dynamics. This topic is of practical relevance to …             Blubber transcriptome response to acute stress axis activation involves transient changes in adipogenesis and lipolysis in a fasting-adapted marine mammal ▶     J. I. Khudyakov, C. D. Champagne, L. M. Meneghetti et al.     Stress can compromise an animal’s ability to conserve metabolic stores and participate in energy-demanding activities that are critical for fitness. Understanding how wild …             Localization of Short-Chain Polyphosphate Enhances its Ability to Clot Flowing Blood Plasma ▶     Ju Hun Yeon, Nima Mazinani, Travis S. Schlappi et al.     Short-chain polyphosphate (polyP) is released from platelets upon platelet activation, but it is not clear if it contributes to thrombosis. PolyP has increased propensity to clot …             Dysregulated autophagy increased melanocyte sensitivity to H2O2-induced oxidative stress in vitiligo ▶     Yuanmin He, Shuli Li, Weigang Zhang et al.     In vitiligo, melanocytes are particularly vulnerable to oxidative stress owing to the pro-oxidant state generated during melanin synthesis and to the genetic antioxidant defects. …             Genetic variants in interferon-λ 4 influences HCV clearance in Chinese Han population ▶     Peng Huang, Yinan Yao, Ming Yue et al.     Recent many studies indicated a novel dinucleotide variant in ss469415590 (TT vs. ΔG) of interferon-λ 4 (IFNL4) gene strongly associated with hepatitis C virus clearance. To …             Corrigendum: HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1 ▶     Valentin Le Douce, Faezeh Forouzanfar, Sebastian Eilebrecht et al.                 Locating Temporal Functional Dynamics of Visual Short-Term Memory Binding using Graph Modular Dirichlet Energy ▶     Keith Smith, Benjamin Ricaud, Nauman Shahid et al.     Visual short-term memory binding tasks are a promising early marker for Alzheimer’s disease (AD). To uncover functional deficits of AD in these tasks it is meaningful to first …             Inhibition of Receptor-Interacting Protein Kinase 1 with Necrostatin–1s ameliorates disease progression in elastase-induced mouse abdominal aortic aneurysm model ▶     Qiwei Wang, Ting Zhou, Zhenjie Liu et al.     Abdominal aortic aneurysm (AAA) is a common aortic disease with a progressive nature. There is no approved pharmacological treatment to effectively slow aneurysm growth or …             Multilocus sequence analyses reveal extensive diversity and multiple origins of fluconazole resistance in Candida tropicalis from tropical China ▶     Jin-Yan Wu, Hong Guo, Hua-Min Wang et al.     Candida tropicalis is among the most prevalent human pathogenic yeast species, second only to C. albicans in certain geographic regions such as East Asia and Brazil. However, …             All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide ▶     Kevin Alessandri, Laetitia Andrique, Maxime Feyeux et al.     While live 3D high resolution microscopy techniques are developing rapidly, their use for biological applications is partially hampered by practical difficulties such as the lack …             Amelioration of experimental autoimmune encephalomyelitis through transplantation of placental derived mesenchymal stem cells ▶     Hong Jiang, Yuanyuan Zhang, Kewei Tian et al.     Placental derived mesenchymal stem cells (PMSCs) have been suggested as a possible source of cells to treat multiple sclerosis (MS) due to their immunomodulatory functions, lack …             Gene Transfer of Prolyl Hydroxylase Domain 2 Inhibits Hypoxia-inducible Angiogenesis in a Model of Choroidal Neovascularization ▶     Anna Takei, Malena Ekström, Parviz Mammadzada et al.     Cellular responses to hypoxia are mediated by the hypoxia-inducible factors (HIF). In normoxia, HIF-α proteins are regulated by a family of dioxygenases, through prolyl and …             Algal Cell Response to Pulsed Waved Stimulation and Its Application to Increase Algal Lipid Production ▶     Oleksandra Savchenko, Jida Xing, Xiaoyan Yang et al.     Generating renewable energy while sequestering CO2 using algae has recently attracted significant research attention, mostly directing towards biological methods such as systems …             Green tea polyphenol tailors cell adhesivity of RGD displaying surfaces: multicomponent models monitored optically ▶     Beatrix Peter, Eniko Farkas, Eniko Forgacs et al.     The interaction of the anti-adhesive coating, poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) and its Arg-Gly-Asp (RGD) functionalized form, PLL-g-PEG-RGD, with the green …             Appropriateness of reference genes for normalizing messenger RNA in mouse 2,4-dinitrobenzene sulfonic acid (DNBS)-induced colitis using quantitative real time PCR ▶     Nour Eissa, Laëtitia Kermarrec, Hayam Hussein et al.     2,4-Dinitrobenzene sulfonic acid (DNBS)-induced colitis is an experimental model that mimics Crohn’s disease. Appropriateness of reference genes is crucial for RT-qPCR. This is …             Corrigendum: Transcriptomic insights into the allelopathic effects of the garlic allelochemical diallyl disulfide on tomato roots ▶     Fang Cheng, Zhi-Hui Cheng, Huan-Wen Meng                 Corrigendum: Kinetic Measurements Reveal Enhanced Protein-Protein Interactions at Intercellular Junctions ▶     Nitesh Shashikanth, Meridith A. Kisting, Deborah E. Leckband                 Sleep habits, academic performance, and the adolescent brain structure ▶     Anna S. Urrila, Eric Artiges, Jessica Massicotte et al.     Here we report the first and most robust evidence about how sleep habits are associated with regional brain grey matter volumes and school grade average in early adolescence. …             Corrigendum: Suppression of B function strongly supports the modified ABCE model in Tricyrtis sp. (Liliaceae) ▶     Masahiro Otani, Ahmad Sharifi, Shosei Kubota et al.                 High-throughput single-cell analysis for the proteomic dynamics study of the yeast osmotic stress response ▶     Rongfei Zhang, Haiyu Yuan, Shujing Wang et al.     Motorized fluorescence microscopy combined with high-throughput microfluidic chips is a powerful method to obtain information about different biological processes in cell biology …             Differences in the transcriptome of medullary thyroid cancer regarding the status and type of RET gene mutations ▶     Malgorzata Oczko-Wojciechowska, Michal Swierniak, Jolanta Krajewska et al.     Medullary thyroid cancer (MTC) can be caused by germline mutations of the RET proto-oncogene or occurs as a sporadic form. It is well known that RET mutations affecting the …             Corrigendum: Vesicle Size Regulates Nanotube Formation in the Cell ▶     Qian Peter Su, Wanqing Du, Qinghua Ji et al.                 Corrigendum: Efficient biosynthesis of a Cecropin A-melittin mutant in Bacillus subtilis WB700 ▶     Shengyue Ji, Weili Li, Abdul Rasheed Baloch et al.                 NF-κB regulates neuronal ankyrin-G via a negative feedback loop ▶     Hans-Georg König, Robert Schwamborn, Silke Andresen et al.     The axon initial segment (AIS) is a neuronal compartment defined by ankyrin-G expression. We here demonstrate that the IKK-complex co-localizes and interacts with the …             Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics ▶     Chiao-Ling Lo, Samrat Roy Choudhury, Joseph Irudayaraj et al.     Enzymes involved in epigenetic processes such as methyltransferases or demethylases are becoming highly utilized for their persistent DNA or histone modifying efficacy. Herein, …             Lumican Peptides: Rational Design Targeting ALK5/TGFBRI ▶     Tarsis Ferreira Gesteira, Vivien J. Coulson-Thomas, Yong Yuan et al.     Lumican, a small leucine rich proteoglycan (SLRP), is a component of extracellular matrix which also functions as a matrikine regulating multiple cell activities. In the cornea, …             Mycorrhizas alter sucrose and proline metabolism in trifoliate orange exposed to drought stress ▶     Hui-Hui Wu, Ying-Ning Zou, Mohammed Mahabubur Rahman et al.     Arbuscular mycorrhizal fungi (AMF) can enhance drought tolerance in plants, whereas little is known regarding AMF contribution to sucrose and proline metabolisms under drought …             Long-term nitrogen fertilization decreased the abundance of inorganic phosphate solubilizing bacteria in an alkaline soil ▶     Bang-Xiao Zheng, Xiu-Li Hao, Kai Ding et al.     Inorganic phosphate solubilizing bacteria (iPSB) are essential to facilitate phosphorus (P) mobilization in alkaline soil, however, the phylogenetic structure of iPSB communities …             Bile acid homeostasis controls CAR signaling pathways in mouse testis through FXRalpha ▶     Emmanuelle Martinot, Marine Baptissart, Aurélie Véga et al.     Bile acids (BAs) are molecules with endocrine activities controlling several physiological functions such as immunity, glucose homeostasis, testicular physiology and male …             Determination of biological activity of gonadotropins hCG and FSH by Förster resonance energy transfer based biosensors ▶     Olga Mazina, Anni Allikalt, Juha S. Tapanainen et al.     Determination of biological activity of gonadotropin hormones is essential in reproductive medicine and pharmaceutical manufacturing of the hormonal preparations. The aim of the …             Mitochondria play an important role in the cell proliferation suppressing activity of berberine ▶     Xiao-Jin Yan, Xuan Yu, Xin-Pei Wang et al.     After being studied for approximately a century, berberine (BBR) has been found to act on various targets and pathways. A great challenge in the pharmacological analysis of BBR …             Genomic insights into the population structure and history of the Irish Travellers ▶     Edmund Gilbert, Shai Carmi, Sean Ennis et al.     The Irish Travellers are a population with a history of nomadism; consanguineous unions are common and they are socially isolated from the surrounding, ‘settled’ Irish people. …             Lack of phylogenetic signals within environmental niches of tropical tree species across life stages ▶     Caicai Zhang, Jie Yang, Liqing Sha et al.     The lasting imprint of phylogenetic history on current day ecological patterns has long intrigued biologists. Over the past decade ecologists have increasingly sought to quantify …             A Floral Fragrance, Methyl Benzoate, is An Efficient Green Pesticide ▶     Yan Feng, Aijun Zhang     Over-reliance on synthetic pesticides in insect pest control has caused widespread public and scientific concerns for human health and the environment, especially since many …             An air-liquid interphase approach for modeling the early embryo-maternal contact zone ▶     S. Chen, S. E. Palma-Vera, M. Langhammer et al.     We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct …             Photoacoustic and Fluorescence Imaging of Cutaneous Squamous Cell Carcinoma in Living Subjects Using a Probe Targeting Integrin αvβ6 ▶     Chao Zhang, Yong Zhang, Kai Hong et al.     Cutaneous squamous cell carcinoma (cSCC) is the second most common non-melanoma skin cancer worldwide. Today, cSCC is diagnosed by visual inspection followed by invasive skin …             Nanoscale diffusion in the synaptic cleft and beyond measured with time-resolved fluorescence anisotropy imaging ▶     Kaiyu Zheng, Thomas P. Jensen, Leonid P. Savtchenko et al.     Neural activity relies on molecular diffusion within nanoscopic spaces outside and inside nerve cells, such as synaptic clefts or dendritic spines. Measuring diffusion on this …             mecA-related structure in methicillin-resistant coagulase-negative staphylococci from street food in Taiwan ▶     Tsung-Ying Yang, Wei-Wen Hung, Lin Lin et al.     Antibiotic-resistant patterns, a mecA homologue complex, and staphylococcal cassette chromosome mec (SCCmec) were analysed in samples of ready-to-eat (RTE) street food in Taiwan. …             Developmental stage-dependent effects of cardiac fibroblasts on function of stem cell-derived engineered cardiac tissues ▶     Brian Liau, Christopher P. Jackman, Yanzhen Li et al.     We investigated whether the developmental stage of mouse cardiac fibroblasts (CFs) influences the formation and function of engineered cardiac tissues made of mouse embryonic …             Ebola virus infection induces autoimmunity against dsDNA and HSP60 ▶     H. Fausther-Bovendo, X. Qiu, S. McCorrister et al.     Ebola virus (EBOV) survivors are affected by a variety of serious illnesses of unknown origin for years after viral clearance from the circulation. Identifying the causes of …             Genome-wide association study for performance traits in chickens using genotype by sequencing approach ▶     Fábio Pértille, Gabriel Costa Monteiro Moreira, Ricardo Zanella et al.     Performance traits are economically important and are targets for selection in breeding programs, especially in the poultry industry. To identify regions on the chicken genome …             Erratum: Biogenic synthesis of Zinc oxide nanostructures from Nigella sativa seed: Prospective role as food packaging material inhibiting broad-spectrum quorum sensing and biofilm ▶     Nasser A. Al-Shabib, Fohad Mabood Husain, Faheem Ahmed et al.                 No effect of mitochondrial genotype on reproductive plasticity following exposure to a non-infectious pathogen challenge in female or male Drosophila ▶     M. Nystrand, E. J. Cassidy, D. K. Dowling     Mitochondrial genetic variation shapes the expression of life-history traits associated with reproduction, development and survival, and has also been associated with the …             MicroRNA-100 is involved in shrimp immune response to white spot syndrome virus (WSSV) and Vibrio alginolyticus infection ▶     Zhi Wang, Fei Zhu     In this study, we discovered that shrimp miR-100 was up-regulated at 24 h after WSSV or Vibrio alginolyticus infection, confirming its participation in the innate immune system …             Using information of relatives in genomic prediction to apply effective stratified medicine ▶     S. Hong Lee, W. M. Shalanee P. Weerasinghe, Naomi R. Wray et al.     Genomic prediction shows promise for personalised medicine in which diagnosis and treatment are tailored to individuals based on their genetic profiles for complex diseases. We …             Optimal asymmetry and other motion parameters that characterise high-quality female dance ▶     Kristofor McCarty, Hannah Darwin, Piers L. Cornelissen et al.     Dance is a universal human behaviour that is observed particularly in courtship contexts, and that provides information that could be useful to potential partners. Here, we use a …             Elongation factor P restricts Salmonella’s growth by controlling translation of a Mg2+ transporter gene during infection ▶     Eunna Choi, Soomin Choi, Daesil Nam et al.     When a ribosome translates mRNA sequences, the ribosome often stalls at certain codons because it is hard to translate. Consecutive proline codons are such examples that induce …             Patterns of pollen and nectar foraging specialization by bumblebees over multiple timescales using RFID ▶     Avery L. Russell, Sarah J. Morrison, Eleni H. Moschonas et al.     The ecological success of social insects is frequently ascribed to improvements in task performance due to division of labour amongst workers. While much research has focused on …             Analysis of drought-responsive signalling network in two contrasting rice cultivars using transcriptome-based approach ▶     Pratikshya Borah, Eshan Sharma, Amarjot Kaur et al.     Traditional cultivars of rice in India exhibit tolerance to drought stress due to their inherent genetic variations. Here we present comparative physiological and transcriptome …             Multivariate approach to quantitative analysis of Aphis gossypii Glover (Hemiptera: Aphididae) and their natural enemy populations at different cotton spacings ▶     José B. Malaquias, Francisco S. Ramalho, Carlos T. dos S. Dias et al.     The relationship between pests and natural enemies using multivariate analysis on cotton in different spacing has not been documented yet. Using multivariate approaches is …             BIM-23A760 influences key functional endpoints in pituitary adenomas and normal pituitaries: molecular mechanisms underlying the differential response in adenomas ▶     Alejandro Ibáñez-Costa, Laura M. López-Sánchez, Manuel D. Gahete et al.     Chimeric somatostatin/dopamine compounds such as BIM-23A760, an sst2/sst5/D2 receptors-agonist, have emerged as promising new approaches to treat pituitary adenomas. However, …             Anti-leukemia effects of the novel synthetic 1-benzylindole derivative 21-900 in vitro and in vivo ▶     Wei-Chun HuangFu, Min-Wu Chao, Chun-Chun Cheng et al.     Cancers are the major cause of death worldwide. Chemotherapy using cytotoxic drugs and targeted therapy is required when surgery is difficult, ineffective, or impossible. We …             Responses of the marine diatom Thalassiosira pseudonana to changes in CO2 concentration: a proteomic approach ▶     Romain Clement, Sabrina Lignon, Pascal Mansuelle et al.     The concentration of CO2 in many aquatic systems is variable, often lower than the KM of the primary carboxylating enzyme Rubisco, and in order to photosynthesize efficiently, …             Structural and Functional insights into the catalytic mechanism of the Type II NADH:quinone oxidoreductase family ▶     Bruno C. Marreiros, Filipa V. Sena, Filipe M. Sousa et al.     Type II NADH:quinone oxidoreductases (NDH-2s) are membrane proteins involved in respiratory chains. These proteins contribute indirectly to the establishment of the transmembrane …             Natriuretic Peptide Receptor B modulates the proliferation of the cardiac cells expressing the Stem Cell Antigen-1 ▶     Stéphanie Rignault-Clerc, Christelle Bielmann, Lucas Liaudet et al.     Brain Natriuretic Peptide (BNP) injections in adult “healthy” or infarcted mice led to increased number of non-myocyte cells (NMCs) expressing the nuclear transcription factor …             Involvement of β-defensin 130 (DEFB130) in the macrophage microbicidal mechanisms for killing Plasmodium falciparum ▶     Mohamad Alaa Terkawi, Ryo Takano, Atsushi Furukawa et al.     Understanding the molecular defense mechanism of macrophages and identifying their effector molecules against malarial parasites may provide important clues for the discovery of …             The NRF2 transcriptional target, OSGIN1, contributes to monomethyl fumarate-mediated cytoprotection in human astrocytes ▶     Melanie S. Brennan, Maria F. Matos, Karl E. Richter et al.     Dimethyl fumarate (DMF) is indicated for the treatment of relapsing multiple sclerosis and may exert therapeutic effects via activation of the nuclear factor (erythroid-derived …             Dissecting the brown adipogenic regulatory network using integrative genomics ▶     Rachana N. Pradhan, Johannes J. Bues, Vincent Gardeux et al.     Brown adipocytes regulate energy expenditure via mitochondrial uncoupling, which makes them attractive therapeutic targets to tackle obesity. However, the regulatory mechanisms …             Gfi1Cre mice have early onset progressive hearing loss and induce recombination in numerous inner ear non-hair cells ▶     Maggie Matern, Sarath Vijayakumar, Zachary Margulies et al.     Studies of developmental and functional biology largely rely on conditional expression of genes in a cell type-specific manner. Therefore, the importance of specificity and lack …             Peripheral sensory neuron injury contributes to neuropathic pain in experimental autoimmune encephalomyelitis ▶     I-Ching Wang, Chen-Yen Chung, Fang Liao et al.     Multiple sclerosis (MS)-induced neuropathic pain deteriorates quality of life in patients but is often refractory to treatment. In experimental autoimmune encephalomyelitis …             Women prefer men who use metaphorical language when paying compliments in a romantic context ▶     Zhao Gao, Shan Gao, Lei Xu et al.     Language plays an important role in romantic attachment. However, it is unclear whether the structure and topic of language use might influence potential mate choice. We …             Structural variability of E. coli thioredoxin captured in the crystal structures of single-point mutants ▶     Martín E. Noguera, Diego S. Vazquez, Gerardo Ferrer-Sueta et al.     Thioredoxin is a ubiquitous small protein that catalyzes redox reactions of protein thiols. Additionally, thioredoxin from E. coli (EcTRX) is a widely-used model for …             Transcriptome profiling of Galaxea fascicularis and its endosymbiont Symbiodinium reveals chronic eutrophication tolerance pathways and metabolic mutualism between partners ▶     Zhenyue Lin, Mingliang Chen, Xu Dong et al.     In the South China Sea, coastal eutrophication in the Beibu Gulf has seriously threatened reef habitats by subjecting corals to chronic physiological stress. To determine how …             Visual cortex and cerebellum hyperactivation during negative emotion picture stimuli in migraine patients ▶     Mengxing Wang, Jingjing Su, Jilei Zhang et al.     Migraines are a common and undertreated disease and often have psychiatric comorbidities; however, the abnormal mechanism of emotional processing in migraine patients has not …             Genetic susceptibility variants for lung cancer: replication study and assessment as expression quantitative trait loci ▶     Giulia Pintarelli, Chiara Elisabetta Cotroneo, Sara Noci et al.     Many single nucleotide polymorphisms (SNPs) have been associated with lung cancer but lack confirmation and functional characterization. We retested the association of 56 …             PARP-1/PARP-2 double deficiency in mouse T cells results in faulty immune responses and T lymphomas ▶     Judith Navarro, Beatriz Gozalbo-López, Andrea C. Méndez et al.     The maintenance of T-cell homeostasis must be tightly regulated. Here, we have identified a coordinated role of Poly(ADP-ribose) polymerase-1 (PARP-1) and PARP-2 in maintaining …             Age-dependent Changes in the Articular Cartilage and Subchondral Bone of C57BL/6 Mice after Surgical Destabilization of Medial Meniscus ▶     Henry Huang, Jordan D. Skelly, David C. Ayers et al.     Age is the primary risk factor for osteoarthritis (OA), yet surgical OA mouse models such as destabilization of the medial meniscus (DMM) used for evaluating disease-modifying OA …             HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1 ▶     Zhihong Yuan, Xian Fan, Bashar Staitieh et al.     Triggering receptor expressed on myeloid cells-1(TREM-1) is a member of the superimmunoglobulin receptor family. We have previously shown that TREM-1 prolongs survival of …             Genomic Variant in IL-37 Confers A Significant Risk of Coronary Artery Disease ▶     Dan Yin, Duraid Hamied Naji, Yunlong Xia et al.     The interleukin 1 family plays an important role in the immune and inflammatory responses. Coronary artery disease (CAD) is a chronic inflammatory disease. However, the genetic …             Multiplexed transcriptome analysis to detect ALK, ROS1 and RET rearrangements in lung cancer ▶     Toni-Maree Rogers, Gisela Mir Arnau, Georgina L. Ryland et al.     ALK, ROS1 and RET gene fusions are important predictive biomarkers for tyrosine kinase inhibitors in lung cancer. Currently, the gold standard method for gene fusion detection is …             Gene variation in IL-7 receptor (IL-7R)α affects IL-7R response in CD4+ T cells in HIV-infected individuals ▶     Hans Jakob Hartling, Lars P. Ryder, Henrik Ullum et al.     Optimal CD4+ T cell recovery after initiating combination antiretroviral treatment (cART) in HIV infection reduces risk of morbidity and mortality. T-allele homozygosity (‘TT’) …             Corrigendum: Structural revisions of small molecules reported to cross-link G-quadruplex DNA in vivo reveal a repetitive assignment error in the literature ▶     Paul E. Reyes-Gutiérrez, Tomáš Kapal, Blanka Klepetářová et al.                 Indispensable role of Mdm2/p53 interaction during the embryonic and postnatal inner ear development ▶     M. Laos, M. Sulg, A. Herranen et al.     p53 is a key component of a signaling network that protects cells against various stresses. As excess p53 is detrimental to cells, its levels are tightly controlled by several …             Fe doped Magnetic Nanodiamonds made by Ion Implantation ▶     ChienHsu Chen, I. C. Cho, Hui-Shan Jian et al.     Here we present a simple physical method to prepare magnetic nanodiamonds (NDs) using high dose Fe ion-implantation. The Fe atoms are embedded into NDs through Fe …             Associations between polymorphisms of the ADIPOQ gene and hypertension risk: a systematic and meta-analysis ▶     Weina Fan, Xiaowei Qu, Jing Li et al.     ADIPOQ gene polymorphisms have been indicated to be associated with hypertension; however, published studies have reported inconsistent results. Eligible studies were retrieved …             Penetration-enhancement underlies synergy of plant essential oil terpenoids as insecticides in the cabbage looper, Trichoplusia ni ▶     Jun-Hyung Tak, Murray B. Isman     Many plant essential oils and their terpenoid constituents possess bioactivities including insecticidal activity, and they sometimes act synergistically when mixed. Although …             The mitotic checkpoint regulator RAE1 induces aggressive breast cancer cell phenotypes by mediating epithelial-mesenchymal transition ▶     Ji Hoon Oh, Ho Hur, Ji-Yeon Lee et al.     The gene RAE1 encodes ribonucleic acid export 1 (RAE1), which is involved in mRNA export and is known to serve as a mitotic checkpoint regulator. In addition, RAE1 …             Sirtuin1 protects endothelial Caveolin-1 expression and preserves endothelial function via suppressing miR-204 and endoplasmic reticulum stress ▶     M. Kassan, A. Vikram, Y. R. Kim et al.     Sirtuin1 (Sirt1) is a class III histone deacetylase that regulates a variety of physiological processes, including endothelial function. Caveolin1 (Cav1) is also an important …             Mediterranean versus Red sea corals facing climate change, a transcriptome analysis ▶     Keren Maor-Landaw, Hiba Waldman Ben-Asher, Sarit Karako-Lampert et al.     The anthropogenic increase in atmospheric CO2 that drives global warming and ocean acidification raises serious concerns regarding the future of corals, the main carbonate …             Analysis of Influenza and RSV dynamics in the community using a ‘Local Transmission Zone’ approach ▶     Gal Almogy, Lewi Stone, B. Andrei Bernevig et al.     Understanding the dynamics of pathogen spread within urban areas is critical for the effective prevention and containment of communicable diseases. At these relatively small …             Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model ▶     Shih-Tien Hsu, Chun-Hsu Yao, Yuan-Man Hsu et al.     Recent studies describe taxol as a candidate treatment for promoting central nerve regeneration. However, taxol has serious side effects including peripheral neurotoxicity, and …             Bigelovin triggered apoptosis in colorectal cancer in vitro and in vivo via upregulating death receptor 5 and reactive oxidative species ▶     Mingyue Li, Li-Hua Song, Grace Gar-Lee Yue et al.     Colorectal cancer (CRC) is the third most prevalent cancer and the third highest cancer-related mortality in the United States. Bigelovin, a sesquiterpene lactone isolated from …             The antimicrobial molecule trappin-2/elafin has anti-parasitic properties and is protective in vivo in a murine model of cerebral malaria ▶     Christian Roussilhon, Gilles Bang, Fabien Bastaert et al.     According to the WHO, and despite reduction in mortality rates, there were an estimated 438 000 malaria deaths in 2015. Therefore new antimalarials capable of limiting organ …             Whole exome sequencing using Ion Proton system enables reliable genetic diagnosis of inherited retinal dystrophies ▶     Marina Riera, Rafael Navarro, Sheila Ruiz-Nogales et al.     Inherited retinal dystrophies (IRD) comprise a wide group of clinically and genetically complex diseases that progressively affect the retina. Over recent years, the development …             Thiazole-valine peptidomimetic (TTT-28) antagonizes multidrug resistance in vitro and in vivo by selectively inhibiting the efflux activity of ABCB1 ▶     Yi-Jun Wang, Bhargav A. Patel, Nagaraju Anreddy et al.     Multidrug resistance (MDR) attenuates the chemotherapy efficacy and increases the probability of cancer recurrence. The accelerated drug efflux mediated by ATP-binding cassette …             A putative MYB35 ortholog is a candidate for the sex-determining genes in Asparagus officinalis ▶     Daisuke Tsugama, Kohei Matsuyama, Mayui Ide et al.     Asparagus officinalis (garden asparagus) is a dioecious perennial crop. For agricultural production of A. officinalis, male plants have advantages over female plants. The …             Cognitive impairment categorized in community-dwelling older adults with and without dementia using in-home sensors that recognise activities of daily living ▶     Prabitha Urwyler, Reto Stucki, Luca Rampa et al.     Cognitive impairment due to dementia decreases functionality in Activities of Daily Living (ADL). Its assessment is useful to identify care needs, risks and monitor disease …             A versatile assay to determine bacterial and host factors contributing to opsonophagocytotic killing in hirudin-anticoagulated whole blood ▶     Erika van der Maten, Marien I. de Jonge, Ronald de Groot et al.     Most bacteria entering the bloodstream will be eliminated through complement activation on the bacterial surface and opsonophagocytosis. However, when these protective innate …             Anhedonia to music and mu-opioids: Evidence from the administration of naltrexone ▶     Adiel Mallik, Mona Lisa Chanda, Daniel J. Levitin     Music’s universality and its ability to deeply affect emotions suggest an evolutionary origin. Previous investigators have found that naltrexone (NTX), a μ-opioid antagonist, may …             Nucleosome Positioning and NDR Structure at RNA Polymerase III Promoters ▶     Alexandra Søgaard Helbo, Fides D. Lay, Peter A. Jones et al.     Chromatin is structurally involved in the transcriptional regulation of all genes. While the nucleosome positioning at RNA polymerase II (pol II) promoters has been extensively …             ISL1 is a major susceptibility gene for classic bladder exstrophy and a regulator of urinary tract development ▶     Rong Zhang, Michael Knapp, Kentaro Suzuki et al.     Previously genome-wide association methods in patients with classic bladder exstrophy (CBE) found association with ISL1, a master control gene expressed in pericloacal …             Comparative study of the gut microbiome potentially related to milk protein in Murrah buffaloes (Bubalus bubalis) and Chinese Holstein cattle ▶     Jiachao Zhang, Chuanbiao Xu, Dongxue Huo et al.     Previous studies suggested a close relationship between ruminant gut microbes and the mammary gland. In this study, shotgun metagenomic sequencing was used to reveal the …             Structure of an octameric form of the minichromosome maintenance protein from the archaeon Pyrococcus abyssi ▶     Giuseppe Cannone, Silvia Visentin, Adeline Palud et al.     Cell division is a complex process that requires precise duplication of genetic material. Duplication is concerted by replisomes. The Minichromosome Maintenance (MCM) replicative …             Motivational, proteostatic and transcriptional deficits precede synapse loss, gliosis and neurodegeneration in the B6.Htt/+Q111/+ model of Huntington’s disease ▶     Robert M. Bragg, Sydney R. Coffey, Rory M. Weston et al.     We investigated the appearance and progression of disease-relevant signs in the B6.HttQ111/+ mouse, a genetically precise model of the mutation that causes Huntington’s disease …             Alleviation of drought stress by mycorrhizas is related to increased root H2O2 efflux in trifoliate orange ▶     Yong-Ming Huang, Ying-Ning Zou, Qiang-Sheng Wu     The Non-invasive Micro-test Technique (NMT) is used to measure dynamic changes of specific ions/molecules non-invasively, but information about hydrogen peroxide (H2O2) fluxes in …             The earliest molecular response to stretch of insect flight muscle as revealed by fast X-ray diffraction recording ▶     Hiroyuki Iwamoto     Small insects drive their flight muscle at frequencies up to 1,000 Hz. This remarkable ability owes to the mechanism of stretch activation. However, it remains unknown as to what …             Targeting the molecular chaperone SlyD to inhibit bacterial growth with a small molecule ▶     Amit Kumar, Jochen Balbach     Molecular chaperones are essential molecules for cell growth, whereby they maintain protein homeostasis. Because of their central cellular function, bacterial chaperones might be …             CXCL12/CXCR4 axis induced miR-125b promotes invasion and confers 5-fluorouracil resistance through enhancing autophagy in colorectal cancer ▶     Xinfeng Yu, Wenna Shi, Yuhang Zhang et al.     The activation of CXCL12/CXCR4 axis is associated with potential progression of cancer, such as invasion, metastasis and chemoresistance. However, the underlying mechanisms of …             Volumetric Spatial Correlations of Neurovascular Coupling Studied using Single Pulse Opto-fMRI ▶     Isabel N. Christie, Jack A. Wells, Sergey Kasparov et al.     Neurovascular coupling describes the link between neuronal activity and cerebral blood flow. This relationship has been the subject of intense scrutiny, with most previous work …             CRISPR/Cas9-mediated gene manipulation to create single-amino-acid-substituted and floxed mice with a cloning-free method ▶     Xiaolong Ma, Chao Chen, Jennifer Veevers et al.     Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is a powerful tool to manipulate the genome with extraordinary …             Integrin α5β1 expression on dopaminergic neurons is involved in dopaminergic neurite outgrowth on striatal neurons ▶     Yasuhiko Izumi, Seiko Wakita, Chisato Kanbara et al.     During development, dopaminergic neurons born in the substantia nigra extend their axons toward the striatum. However, the mechanisms by which the dopaminergic axons extend the …             Decreased expression of STING predicts poor prognosis in patients with gastric cancer ▶     Shushu Song, Peike Peng, Zhaoqing Tang et al.     STING (stimulator of interferon genes) has recently been found to play an important role in host defenses against virus and intracellular bacteria via the regulation of type-I …             Metagenomic survey of the taxonomic and functional microbial communities of seawater and sea ice from the Canadian Arctic ▶     Etienne Yergeau, Christine Michel, Julien Tremblay et al.     Climate change has resulted in an accelerated decline of Arctic sea ice since 2001 resulting in primary production increases and prolongation of the ice-free season within the …             Genotoxic Effects of Culture Media on Human Pluripotent Stem Cells ▶     Megha Prakash Bangalore, Syama Adhikarla, Odity Mukherjee et al.     Culture conditions play an important role in regulating the genomic integrity of Human Pluripotent Stem Cells (HPSCs). We report that HPSCs cultured in Essential 8 (E8) and …             Arbuscular mycorrhiza enhance the rate of litter decomposition while inhibiting soil microbial community development ▶     Heng Gui, Kevin Hyde, Jianchu Xu et al.     Although there is a growing amount of evidence that arbuscular mycorrhizal fungi (AMF) influence the decomposition process, the extent of their involvement remains unclear. …             Effect of domestication on the genetic diversity and structure of Saccharina japonica populations in China ▶     Jie Zhang, Xiuliang Wang, Jianting Yao et al.     Saccharina japonica is a commercially and ecologically important seaweed and is an excellent system for understanding the effects of domestication on marine crops. In this study, …             Application of Machine-Learning Models to Predict Tacrolimus Stable Dose in Renal Transplant Recipients ▶     Jie Tang, Rong Liu, Yue-Li Zhang et al.     Tacrolimus has a narrow therapeutic window and considerable variability in clinical use. Our goal was to compare the performance of multiple linear regression (MLR) and eight …             ePro-ClearSee: a simple immunohistochemical method that does not require sectioning of plant samples ▶     Kiyotaka Nagaki, Naoki Yamaji, Minoru Murata     Investigations into the epigenetic status of individual cells within tissues can produce both epigenetic data for different cell types and positional information of the cells. …             Quantifying tumor associated macrophages in breast cancer: a comparison of iron and fluorine-based MRI cell tracking ▶     Ashley V. Makela, Jeffrey M. Gaudet, Paula J. Foster     Tumor associated macrophages (TAMs) are associated with tumor growth and metastasis. MRI can detect TAMs labeled with iron oxide (USPIO) or perfluorocarbon (PFC) agents. This …             Mechanisms responsible for the synergistic antileukemic interactions between ATR inhibition and cytarabine in acute myeloid leukemia cells ▶     Jun Ma, Xinyu Li, Yongwei Su et al.     Acute myeloid leukemia (AML) continues to be a challenging disease to treat, thus new treatment strategies are needed. In this study, we investigated the antileukemic effects of …             Transmissible endoplasmic reticulum stress from myocardiocytes to macrophages is pivotal for the pathogenesis of CVB3-induced viral myocarditis ▶     Hui Zhang, Yan Yue, Tianle Sun et al.     Infiltrating macrophages have been proven as a pivotal pathological inflammatory cell subset in coxsackievirus B3 (CVB3) induced viral myocarditis. However, the mechanisms …             Structural basis for redox sensitivity in Corynebacterium glutamicum diaminopimelate epimerase: an enzyme involved in l-lysine biosynthesis ▶     Hye-Young Sagong, Kyung-Jin Kim     Diaminopimelate epimerase (DapF) is one of the crucial enzymes involved in l-lysine biosynthesis, where it converts l,l-diaminopimelate (l,l-DAP) into d,l-DAP. DapF is also …             Cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum ▶     Jinhai Huang, Yao Wu, Wenjun Wu et al.     Production of 1,3-propanediol (1,3-PDO) from glycerol is a promising route toward glycerol biorefinery. However, the yield of 1,3-PDO is limited due to the requirement of NADH …             Intestinal cancer stem cells marked by Bmi1 or Lgr5 expression contribute to tumor propagation via clonal expansion ▶     Hirotsugu Yanai, Naho Atsumi, Toshihiro Tanaka et al.     Although the existence of cancer stem cells in intestine tumors has been suggested, direct evidence has not been yet provided. Here, we showed, using the multicolor …             A combinational CRISPR/Cas9 gene-editing approach can halt HIV replication and prevent viral escape ▶     Robert Jan Lebbink, Dorien C. M. de Jong, Femke Wolters et al.     HIV presents one of the highest evolutionary rates ever detected and combination antiretroviral therapy is needed to overcome the plasticity of the virus population and control …             Massively parallel digital high resolution melt for rapid and absolutely quantitative sequence profiling ▶     Daniel Ortiz Velez, Hannah Mack, Julietta Jupe et al.     In clinical diagnostics and pathogen detection, profiling of complex samples for low-level genotypes represents a significant challenge. Advances in speed, sensitivity, and …             Structural basis of the interaction between Topoisomerase IIIβ and the TDRD3 auxiliary factor ▶     Sakurako Goto-Ito, Atsushi Yamagata, Tomio S. Takahashi et al.     Topoisomerase IIIβ (TOP3β) is a DNA/RNA topoisomerase that has been implicated in epigenetic or translational control of gene expression. In cells, TOP3β co-exists with its …             N,N Dimethylacetamide a drug excipient that acts as bromodomain ligand for osteoporosis treatment ▶     Chafik Ghayor, Bebeka Gjoksi, Jing Dong et al.     N,N-Dimethylacetamide (DMA) is a water-miscible solvent, FDA approved as excipient and therefore widely used as drug-delivery vehicle. As such, DMA should be devoid of any …             A critical role of platelet TGF-β release in podoplanin-mediated tumour invasion and metastasis ▶     Ai Takemoto, Mina Okitaka, Satoshi Takagi et al.     The tumour microenvironment is critical for various characteristics of tumour malignancies. Platelets, as part of the tumour microenvironment, are associated with metastasis …             eUnaG: a new ligand-inducible fluorescent reporter to detect drug transporter activity in live cells ▶     Johannes T.-H. Yeh, Kwangho Nam, Joshua T.-H. Yeh et al.     The absorption, distribution, metabolism and excretion (ADME) of metabolites and toxic organic solutes are orchestrated by the ATP-binding cassette (ABC) transporters and the …             Quantification of Detergents Complexed with Membrane Proteins ▶     Vincent Chaptal, Frédéric Delolme, Arnaud Kilburg et al.     Most membrane proteins studies require the use of detergents, but because of the lack of a general, accurate and rapid method to quantify them, many uncertainties remain that …             Impact of Including Peritumoral Edema in Radiotherapy Target Volume on Patterns of Failure in Glioblastoma following Temozolomide-based Chemoradiotherapy ▶     Seo Hee Choi, Jun Won Kim, Jee Suk Chang et al.     We assessed the impact of including peritumoral edema in radiotherapy volumes on recurrence patterns among glioblastoma multiforme (GBM) patients treated with standard …             Structural insights into human angiogenin variants implicated in Parkinson’s disease and Amyotrophic Lateral Sclerosis ▶     William J. Bradshaw, Saima Rehman, Tram T. K. Pham et al.     Mutations in Angiogenin (ANG), a member of the Ribonuclease A superfamily (also known as RNase 5) are known to be associated with Amyotrophic Lateral Sclerosis (ALS, motor …             The dynamic mechanism of noisy signal decoding in gene regulation ▶     Peijiang Liu, Haohua Wang, Lifang Huang et al.     Experimental evidence supports that signaling pathways can induce different dynamics of transcription factor (TF) activation, but how an input signal is encoded by such a …             Mycobacterium tuberculosis exploits the PPM1A signaling pathway to block host macrophage apoptosis ▶     Kaitlyn Schaaf, Samuel R. Smith, Alexandra Duverger et al.     The ability to suppress host macrophage apoptosis is essential for M. tuberculosis (Mtb) to replicate intracellularly while protecting it from antibiotic treatment. We recently …             MFN2 suppresses cancer progression through inhibition of mTORC2/Akt signaling ▶     Ke Xu, Guo Chen, Xiaobo Li et al.     The mitochondrial GTPase mitofusin-2 (MFN2) has previously been reported to play a role in regulating cell proliferation, apoptosis and differentiation in a number of cell types. …             Examining brain structures associated with dispositional envy and the mediation role of emotional intelligence ▶     Yanhui Xiang, Sasa Zhao, Hanlin Wang et al.     Dispositional envy is distinguished by definition and neurally from episodic envy. While the neural correlates of episodic envy have been evaluated by specific tasks in previous …             AMPK regulates lipid accumulation in skeletal muscle cells through FTO-dependent demethylation of N6-methyladenosine ▶     Weiche Wu, Jie Feng, Denghu Jiang et al.     Skeletal muscle plays important roles in whole-body energy homeostasis. Excessive skeletal muscle lipid accumulation is associated with some metabolic diseases such as obesity …             Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis ▶     Nuria Andreu, Jody Phelan, Paola F. de Sessions et al.     Macrophages play an essential role in the early immune response to Mycobacterium tuberculosis and are the cell type preferentially infected in vivo. Primary macrophages and …             DoGMP1 from Dendrobium officinale contributes to mannose content of water-soluble polysaccharides and plays a role in salt stress response ▶     Chunmei He, Zhenming Yu, Jaime A. Teixeira da Silva et al.     GDP-mannose pyrophosphorylase (GMP) catalyzed the formation of GDP-mannose, which serves as a donor for the biosynthesis of mannose-containing polysaccharides. In this study, …             Marine Streptomyces sp. derived antimycin analogues suppress HeLa cells via depletion HPV E6/E7 mediated by ROS-dependent ubiquitin–proteasome system ▶     Weiyi Zhang, Qian Che, Hongsheng Tan et al.     Four new antimycin alkaloids (1–4) and six related known analogs (5–10) were isolated from the culture of a marine derived Streptomyces sp. THS-55, and their structures were …             Transcriptional firing helps to drive NETosis ▶     Meraj A. Khan, Nades Palaniyar     Neutrophils are short-lived innate immune cells. These cells respond quickly to stimuli, and die within minutes to hours; the relevance of DNA transcription in dying neutrophils …             Extinctions, genetic erosion and conservation options for the black rhinoceros (Diceros bicornis) ▶     Yoshan Moodley, Isa-Rita M. Russo, Desiré L. Dalton et al.     The black rhinoceros is again on the verge of extinction due to unsustainable poaching in its native range. Despite a wide historic distribution, the black rhinoceros was …             Reduction of Abeta amyloid pathology in APPPS1 transgenic mice in the absence of gut microbiota ▶     T. Harach, N. Marungruang, N. Duthilleul et al.     Alzheimer’s disease is the most common form of dementia in the western world, however there is no cure available for this devastating neurodegenerative disorder. Despite clinical …             Engineering a pH responsive pore forming protein ▶     Matic Kisovec, Saša Rezelj, Primož Knap et al.     Listeriolysin O (LLO) is a cytolysin capable of forming pores in cholesterol-rich lipid membranes of host cells. It is conveniently suited for engineering a pH-governed …             Synthesis, Fungicidal Activity, and Structure Activity Relationship of β-Acylaminocycloalkylsulfonamides against Botrytis cinerea ▶     Chun-Hui Liu, Xiao-Yuan Chen, Pei-Wen Qin et al.     In order to discover new antifungal agrochemicals that could have highly active and novel motifs, thirty-six new 2-acylaminocycloalkylsulfonamides (IV) were synthesized. Their …             Griseofulvin impairs intraerythrocytic growth of Plasmodium falciparum through ferrochelatase inhibition but lacks activity in an experimental human infection study ▶     Clare M. Smith, Ante Jerkovic, Thy Thuc Truong et al.     Griseofulvin, an orally active antifungal drug used to treat dermatophyte infections, has a secondary effect of inducing cytochrome P450-mediated production of N-methyl …             Prediction of biomarkers of oral squamous cell carcinoma using microarray technology ▶     Guang Li, Xian Li, Meng Yang et al.     Microarray data is used to screen the genes of oral squamous cell carcinoma (OSCC). Microarray data of OSCC and normal tissues were downloaded from GEO database and analyzed with …             Respiratory impedance is correlated with morphological changes in the lungs on three-dimensional CT in patients with COPD ▶     Masato Karayama, Naoki Inui, Kazutaka Mori et al.     The forced oscillation technique provides information concerning respiratory impedance, which comprises resistance and reactance of the respiratory system. However, its …             Historical factors shaped species diversity and composition of Salix in eastern Asia ▶     Qinggang Wang, Xiangyan Su, Nawal Shrestha et al.     Ambient energy, niche conservatism, historical climate stability and habitat heterogeneity hypothesis have been proposed to explain the broad-scale species diversity patterns and …             Annexin-A5 organized in 2D-network at the plasmalemma eases human trophoblast fusion ▶     Severine A. Degrelle, Pascale Gerbaud, Ludovic Leconte et al.     Only a limited number of human cells can fuse to form a multinucleated syncytium. Cell fusion occurs as part of the differentiation of some cell types, including myotubes in …             Ocular Reflex Phase during Off-Vertical Axis Rotation in Humans is Modified by Head-Turn-On-Trunk Position ▶     Samantha B. Douglas, Gilles Clément, Pierre Denise et al.     Constant velocity Off-Vertical Axis Rotation (OVAR) imposes a continuously varying orientation of the head and body relative to gravity, which generates a modulation of …             Modified forelimb grip strength test detects aging-associated physiological decline in skeletal muscle function in male mice ▶     Hikari Takeshita, Koichi Yamamoto, Satoko Nozato et al.     The conventional forelimb grip strength test is a widely used method to assess skeletal muscle function in rodents; in this study, we modified this method to improve its …             Distribution bias analysis of germline and somatic single-nucleotide variations that impact protein functional site and neighboring amino acids ▶     Yang Pan, Cheng Yan, Yu Hu et al.     Single nucleotide variations (SNVs) can result in loss or gain of protein functional sites. We analyzed the effects of SNVs on enzyme active sites, ligand binding sites, and …             Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells ▶     Jun-Seok Oh, Shinya Kojima, Minoru Sasaki et al.     We have developed a plasma cell treatment device called Plasma-on-Chip that enables the real-time monitoring of a single cell culture during plasma treatment. The device consists …             Spatial variation and inconsistency between estimates of onset of muscle activation from EMG and ultrasound ▶     Angela V. Dieterich, Alberto Botter, Taian Martins Vieira et al.     Delayed onset of muscle activation can be a descriptor of impaired motor control. Activation onset can be estimated from electromyography (EMG)-registered muscle excitation and …             PARP inhibitor increases chemosensitivity by upregulating miR-664b-5p in BRCA1-mutated triple-negative breast cancer ▶     Wei Song, Lin Tang, Yumei Xu et al.     Emerging evidence has shown that adding poly(ADP-ribose) polymerase (PARP) inhibitors to chemotherapy regimens is superior to the control regimens alone in BRCA1-mutated …             Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease ▶     Dirk Hubmacher, Michael Schneider, Steven J. Berardinelli et al.     Secreted metalloproteases have diverse roles in the formation, remodeling, and the destruction of extracellular matrix. Recessive mutations in the secreted metalloprotease …             Functional Coupling of Human Microphysiology Systems: Intestine, Liver, Kidney Proximal Tubule, Blood-Brain Barrier and Skeletal Muscle ▶     Lawrence Vernetti, Albert Gough, Nicholas Baetz et al.     Organ interactions resulting from drug, metabolite or xenobiotic transport between organs are key components of human metabolism that impact therapeutic action and toxic side …             Nitrate leaching in a winter wheat-summer maize rotation on a calcareous soil as affected by nitrogen and straw management ▶     Tao Huang, Xiaotang Ju, Hao Yang     Nitrate leaching is one of the most important pathways of nitrogen (N) loss which leads to groundwater contamination or surface water eutrophication. Clarifying the rates, …             Comparative Study of the Collective Dynamics of Proteins and Inorganic Nanoparticles ▶     Esmael J. Haddadian, Hao Zhang, Karl F. Freed et al.     Molecular dynamics simulations of ubiquitin in water/glycerol solutions are used to test the suggestion by Karplus and coworkers that proteins in their biologically active state …             Characterization of Adipogenic Chemicals in Three Different Cell Culture Systems: Implications for Reproducibility Based on Cell Source and Handling ▶     Christopher D. Kassotis, Lauren Masse, Stephanie Kim et al.     The potential for chemical exposures to exacerbate the development and/or prevalence of metabolic disorders, such as obesity, is currently of great societal concern. Various in …             Three-dimensional aligned nanofibers-hydrogel scaffold for controlled non-viral drug/gene delivery to direct axon regeneration in spinal cord injury treatment ▶     Lan Huong Nguyen, Mingyong Gao, Junquan Lin et al.     Spinal cord injuries (SCI) often lead to persistent neurological dysfunction due to failure in axon regeneration. Unfortunately, currently established treatments, such as direct …             Novel peptides for deciphering structural and signalling functions of E-cadherin in mouse embryonic stem cells ▶     Joe M. Segal, Christopher M. Ward     We have previously shown that E-cadherin regulates the naive pluripotent state of mouse embryonic stem cells (mESCs) by enabling LIF-dependent STAT3 phosphorylation, with …             Ultrasound-Mediated Mesenchymal Stem Cells Transfection as a Targeted Cancer Therapy Platform ▶     Tom Haber, Limor Baruch, Marcelle Machluf     Mesenchymal stem cells (MSCs) hold tremendous potential as a targeted cell-based delivery platform for inflammatory and cancer therapy. Genetic manipulation of MSCs, however, is …             The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase Function ▶     Shan He, Yu Cao, Ping Xie et al.     Nedd8 is a ubiquitin-like protein that controls vital biological events through conjugation to target proteins. We previously identified the HECT-type ubiquitin ligase Smurf1 …             Searching for new loci and candidate genes for economically important traits through gene-based association analysis of Simmental cattle ▶     Jiangwei Xia, Huizhong Fan, Tianpeng Chang et al.     Single-marker genome-wide association study (GWAS) is a convenient strategy of genetic analysis that has been successful in detecting the association of a number of …             Collagen scaffold microenvironments modulate cell lineage commitment for differentiation of bone marrow cells into regulatory dendritic cells ▶     Yongxiang Fang, Bin Wang, Yannan Zhao et al.     The microenvironment plays a pivotal role for cell survival and functional regulation, and directs the cell fate determination. The biological functions of DCs have been …             The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression ▶     I. Gonzalez-Vasconcellos, R. Schneider, N. Anastasov et al.     The tumour suppressor gene (Rb1) is necessary for the maintenance of telomere integrity in osteoblastic cells. We now show that the compaction of telomeric chromatin and the …             Loss of DLG5 promotes breast cancer malignancy by inhibiting the Hippo signaling pathway ▶     Jie Liu, Juan Li, Pingping Li et al.     Discs Large Homolog 5 (DLG5) plays an important role in the maintenance of epithelial cell polarity. Recent research showed that DLG5 is decreased in Yes-associated protein …             A sugarcane R2R3-MYB transcription factor gene is alternatively spliced during drought stress ▶     Jinlong Guo, Hui Ling, Jingjing Ma et al.     MYB transcription factors of the R2R3-MYB family have been shown to play important roles in many plant processes. A sugarcane R2R3-MYB gene (ScMYB2) and its two alternative forms …             DNA methylation governs the dynamic regulation of inflammation by apoptotic cells during efferocytosis ▶     Clare A. Notley, Christine K. Jordan, Jenny L. McGovern et al.     Efficient clearance of apoptotic cells (AC) is pivotal in preventing autoimmunity and is a potent immunosuppressive stimulus. However, activation of cells prior to apoptosis …             Attenuation of lymphocyte immune responses during Mycobacterium avium complex-induced lung disease due to increasing expression of programmed death-1 on lymphocytes ▶     Chin-Chung Shu, Jann-Yuan Wang, Ming-Fang Wu et al.     Mycobacterium avium complex-induced lung disease (MAC-LD) becomes important due to its increasing prevalence. Attenuated cellular immunity associated with programmed cell death …             Anti-apoptotic effects of Sonic hedgehog signalling through oxidative stress reduction in astrocytes co-cultured with excretory-secretory products of larval Angiostrongylus cantonensis ▶     Kuang-Yao Chen, Cheng-Hsun Chiu, Lian-Chen Wang     Angiostrongylus cantonensis, the rat lungworm, is an important aetiologic agent of eosinophilic meningitis and meningoencephalitis in humans. Co-culturing astrocytes with soluble …             Human CD64-targeted non-viral siRNA delivery system for blood monocyte gene modulation ▶     Seok-Beom Yong, Hyung Jin Kim, Jang Kyoung Kim et al.     A subset of phagocytes including inflammatory monocytes in blood migrate and give rise to macrophages in inflammatory tissues which generated the idea that blood monocytes are …             Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer ▶     Elena Andretta, Fernando Cartón-García, Águeda Martínez-Barriocanal et al.     EPH signaling deregulation has been shown to be important for colorectal carcinogenesis and genome-wide sequencing efforts have identified EPHA3 as one of the most frequently …             Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli ▶     Shuangtao Li, Lei Zhang, Ying Wang et al.     Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse …             Correlative Imaging of the Murine Hind Limb Vasculature and Muscle Tissue by MicroCT and Light Microscopy ▶     Laura Schaad, Ruslan Hlushchuk, Sébastien Barré et al.     A detailed vascular visualization and adequate quantification is essential for the proper assessment of novel angiomodulating strategies. Here, we introduce an ex vivo …             Accelerating Corrosion of Pure Magnesium Co-implanted with Titanium in Vivo ▶     Peng Hou, Pei Han, Changli Zhao et al.     Magnesium is a type of reactive metal, and is susceptible to galvanic corrosion. In the present study, the impact of coexistence of Ti on the corrosion behavior of high purity Mg …             Magnetoresistive sensors for measurements of DNA hybridization kinetics – effect of TINA modifications ▶     G. Rizzi, M. Dufva, M. F. Hansen     We present the use of magnetoresistive sensors integrated in a microfluidic system for real-time studies of the hybridization kinetics of DNA labeled with magnetic nanoparticles …             Effect of exogenous selenium supply on photosynthesis, Na+ accumulation and antioxidative capacity of maize (Zea mays L.) under salinity stress ▶     Chaoqiang Jiang, Chaolong Zu, Dianjun Lu et al.     The mechanism of selenium-mediated salt tolerance has not been fully clarified. This study investigated the possible role of selenium (Se) in regulating maize salt tolerance. A …             Assessing of the contributions of pod photosynthesis to carbon acquisition of seed in alfalfa (Medicago sativa L.) ▶     Wenxu Zhang, Peisheng Mao, Yuan Li et al.     The distribution of carbon from a branch setting pod in alfalfa was investigated during the seed development of seeds to determine the relative contribution of pod and leaf …             A new perspective on studying burial environment before archaeological excavation: analyzing bacterial community distribution by high-throughput sequencing ▶     Jinjin Xu, Yanfei Wei, Hanqing Jia et al.     Burial conditions play a crucial role in archaeological heritage preservation. Especially, the microorganisms were considered as the leading causes which incurred degradation and …             The internal anatomy of titanosaur osteoderms from the Upper Cretaceous of Spain is compatible with a role in oogenesis ▶     Daniel Vidal, Francisco Ortega, Francisco Gascó et al.     Dermal armor is one of the most intriguing features of some titanosaurs, the only sauropod dinosaurs that bore osteoderms. Some studies have revealed cavities of varying sizes …             Secreted frizzled-related protein 1 overexpression in gastric cancer: Relationship with radiological findings of dual-energy spectral CT and PET-CT ▶     Huimin Lin, Guoyuan Yang, Bei Ding et al.     We explored the role of secreted frizzled-related protein 1 (sFRP1) overexpression in gastric cancer and its relationship with radiological findings from dual-energy spectral …             Tai Chi Chuan and Baduanjin practice modulates functional connectivity of the cognitive control network in older adults ▶     Jing Tao, Xiangli Chen, Natalia Egorova et al.     Cognitive impairment is one of the most common problem saffecting older adults. In this study, we investigated whether Tai Chi Chuan and Baduanjin practice can modulate mental …             Enhancement of 5-aminolevulinic acid-based fluorescence detection of side population-defined glioma stem cells by iron chelation ▶     Wenqian Wang, Kouichi Tabu, Yuichiro Hagiya et al.     Cancer stem cells (CSCs) are dominantly responsible for tumor progression and chemo/radio-resistance, resulting in tumor recurrence. 5-aminolevulinic acid (ALA) is metabolized to …             Visualization of tumor-related blood vessels in human breast by photoacoustic imaging system with a hemispherical detector array ▶     M. Toi, Y. Asao, Y. Matsumoto et al.     Noninvasive measurement of the distribution and oxygenation state of hemoglobin (Hb) inside the tissue is strongly required to analyze the tumor-associated vasculatures. We …             Cross-modal noise compensation in audiovisual words ▶     Martijn Baart, Blair C. Armstrong, Clara D. Martin et al.     Perceiving linguistic input is vital for human functioning, but the process is complicated by the fact that the incoming signal is often degraded. However, humans can compensate …             Chloroquine inhibits human CD4+ T-cell activation by AP-1 signaling modulation ▶     Ralf L. J. Schmidt, Sabrina Jutz, Katrin Goldhahn et al.     Chloroquine (CQ) is widely used as an anti-inflammatory therapeutic for rheumatic diseases. Although its modes of action on the innate immune system are well described, there is …             Social pain and social gain in the adolescent brain: A common neural circuitry underlying both positive and negative social evaluation ▶     Tim Dalgleish, Nicholas D. Walsh, Dean Mobbs et al.     Social interaction inherently involves the subjective evaluation of cues salient to social inclusion and exclusion. Testifying to the importance of such social cues, parts of the …             Shrimp AHPND-causing plasmids encoding the PirAB toxins as mediated by pirAB-Tn903 are prevalent in various Vibrio species ▶     Jinzhou Xiao, Liyuan Liu, Yiyun Ke et al.     Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease caused by pirAB toxins encoded by a plasmid found in Vibrio parahaemolyticus. The pirAB toxins …             Novel benzoxazole derivatives DCPAB and HPAB attenuate Th1 cell-mediated inflammation through T-bet suppression ▶     Yeon Ji Oh, Darong Kim, Sera Oh et al.     Interferon-γ (IFN-γ), a critical inflammatory cytokine, is primarily produced by T helper 1 (Th1) cells and accelerates the pathogenesis of inflammatory colitis. Pharmacological …             Stoichiometry and structure of a lantibiotic maturation complex ▶     Jens Reiners, André Abts, Rebecca Clemens et al.     Lantibiotics are ribosomally synthesized antimicrobial peptides secreted by mainly Gram-positive bacteria. Class 1 lantibiotics mature via two modification steps introduced by a …             Copulatory courtship by internal genitalia in bushcrickets ▶     Nadja C. Wulff , Thomas van de Kamp, Tomy dos Santos Rolo et al.     Male genital organs are among the fastest evolving morphological structures. However, large parts of the male’s genitalia are often hidden inside the female during mating. In …             RNA-Seq analysis of nodule development at five different developmental stages of soybean (Glycine max) inoculated with Bradyrhizobium japonicum strain 113-2 ▶     Song L. Yuan, Rong Li, Hai F. Chen et al.     Nodule development directly affects nitrogen fixation efficiency during soybean growth. Although abundant genome-based information related to nodule development has been released …             Physiological and transcriptomic analyses reveal a response mechanism to cold stress in Santalum album L. leaves ▶     Xinhua Zhang, Jaime A. Teixeira da Silva, Meiyun Niu et al.     Santalum album L. (Indian sandalwood) is an economically important plant species because of its ability to produce highly valued perfume oils. Little is known about the …             Gating of memory encoding of time-delayed cross-frequency MEG networks revealed by graph filtration based on persistent homology ▶     Jarang Hahm, Hyekyoung Lee, Hyojin Park et al.     To explain gating of memory encoding, magnetoencephalography (MEG) was analyzed over multi-regional network of negative correlations between alpha band power during cue …             Early-life stress impairs recognition memory and perturbs the functional maturation of prefrontal-hippocampal-perirhinal networks ▶     Samuel A. J. Reincke, Ileana L. Hanganu-Opatz     Early life exposure to stressful situations impairs cognitive performance of adults and contributes to the etiology of several psychiatric disorders. Most of affected cognitive …             Sequestration of Ribosome during Protein Aggregate Formation: Contribution of ribosomal RNA ▶     Bani K. Pathak, Surojit Mondal, Senjuti Banerjee et al.     An understanding of the mechanisms underlying protein aggregation and cytotoxicity of the protein aggregates is crucial in the prevention of several diseases in humans. Ribosome, …             SpotLight Proteomics: uncovering the hidden blood proteome improves diagnostic power of proteomics ▶     Susanna L. Lundström, Bo Zhang, Dorothea Rutishauser et al.     The human blood proteome is frequently assessed by protein abundance profiling using a combination of liquid chromatography and tandem mass spectrometry (LC-MS/MS). In …             Loss of HSulf-1: The Missing Link between Autophagy and Lipid Droplets in Ovarian Cancer ▶     Debarshi Roy, Susmita Mondal, Ashwani Khurana et al.     Defective autophagy and deranged metabolic pathways are common in cancer; pharmacologic targeting of these two pathways could provide a viable therapeutic option. However, how …             Enhanced delignification of steam-pretreated poplar by a bacterial laccase ▶     Rahul Singh, Jinguang Hu, Matthew R. Regner et al.     The recalcitrance of woody biomass, particularly its lignin component, hinders its sustainable transformation to fuels and biomaterials. Although the recent discovery of several …             Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca2+-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation ▶     Ying Zhang, Min Zhang, Bochao Lyu et al.     We previously reported that eicosapentaenoic acid (EPA), an omega-3 polyunsaturated fatty acid (n-3 PUFA), effectively inhibits sphingosylphosphorylcholine (SPC)-induced Ca2+…             Genome-wide analysis of auxin transport genes identifies the hormone responsive patterns associated with leafy head formation in Chinese cabbage ▶     Li-wei Gao, Shan-wu Lyu, Jun Tang et al.     Auxin resistant 1/like aux1 (AUX/LAX), pin-formed (PIN) and ATP binding cassette subfamily B (ABCB/MDR/PGP) are three families of auxin transport genes. The development-related …             Bronchoalveolar Lavage Proteomics in Patients with Suspected Lung Cancer ▶     Ana Sofia Carvalho, Célia Marina Cuco, Carla Lavareda et al.     Lung cancer configures as one of the deadliest types of cancer. The future implementation of early screening methods such as exhaled breath condensate analysis and low dose …             Improving the therapeutic efficacy of mesenchymal stromal cells to restore perfusion in critical limb ischemia through pulsed focused ultrasound ▶     Pamela A. Tebebi, Saejeong J. Kim, Rashida A. Williams et al.     Mesenchymal stem cells (MSC) are promising therapeutics for critical limb ischemia (CLI). Mechanotransduction from pulsed focused ultrasound (pFUS) upregulates local …             Multiple tropical Andean glaciations during a period of late Pliocene warmth ▶     Nicholas J. Roberts, René W. Barendregt, John J. Clague     The extent and behaviour of glaciers during the mid-Piacenzian warm period illustrate the sensitivity of the cryosphere to atmospheric CO2 concentrations above pre-industrial …             Quality Evaluation Focusing on Tissue Fractal Dimension and Chemical Changes for Frozen Tilapia with Treatment by Tangerine Peel Extract ▶     Qi He, Zhao Yang, Bin Gong et al.     This work aimed to establish an effective approach to evaluate the quality of frozen fish, focusing on changes in fish tissue structure and chemical composition during storage. …             Metabolomic profiling of reactive persulfides and polysulfides in the aqueous and vitreous humors ▶     Hiroshi Kunikata, Tomoaki Ida, Kota Sato et al.     We investigate the metabolomic profile of reactive persulfides and polysulfides in the aqueous and vitreous humors. Eighteen eyes of 18 consecutive patients with diabetes …             Variation among conventional cultivars could be used as a criterion for environmental safety assessment of Bt rice on nontarget arthropods ▶     Fang Wang, Cong Dang, Xuefei Chang et al.     The current difficulty facing risk evaluations of Bacillus thuringiensis (Bt) crops on nontarget arthropods (NTAs) is the lack of criteria for determining what represents …             Cytoprotective effects of mild plasma-activated medium against oxidative stress in human skin fibroblasts ▶     Minori Horiba, Tetsuro Kamiya, Hirokazu Hara et al.     Non-thermal atmospheric pressure plasma (NTAPP) has recently been applied to living cells and tissues and has emerged as a novel technology for medical applications. NTAPP …             Phytoalexin Phenalenone Derivatives Inactivate Mosquito Larvae and Root-knot Nematode as Type-II Photosensitizer ▶     Runjiang Song, Yian Feng, Donghui Wang et al.     Phytoalexins phenalenones (PNs) are phytochemicals biosynthesized inside the plant in responsive to exterior threat. PNs are excellent type-II photosensitizers, which efficiently …             Integrative analysis of copy number and transcriptional expression profiles in esophageal cancer to identify a novel driver gene for therapy ▶     Gaochao Dong, Qixing Mao, Decai Yu et al.     An increasing amount of evidence has highlighted the critical roles that copy number variants play in cancer progression. Here, we systematically analyzed the copy number …             Allometric scaling in-vitro ▶     Arti Ahluwalia     About two decades ago, West and coworkers established a model which predicts that metabolic rate follows a three quarter power relationship with the mass of an organism, based on …             Pigment epithelium-derived factor attenuates myocardial fibrosis via inhibiting Endothelial-to-Mesenchymal Transition in rats with acute myocardial infarction ▶     Hao Zhang, Hongliang Hui, Zhimin Li et al.     Endothelial mesenchymal transition (EndMT) plays a critical role in the pathogenesis and progression of interstitial and perivascular fibrosis after acute myocardial infarction …             Growth-inducing effects of argon plasma on soybean sprouts via the regulation of demethylation levels of energy metabolism-related genes ▶     Jiao Jiao Zhang, Jin Oh Jo, Do Luong Huynh et al.     This study was conducted to determine the effects of argon plasma on the growth of soybean [Glycine max (L.) Merr.] sprouts and investigate the regulation mechanism of energy …             Noninvasive and quantitative intracranial pressure estimation using ultrasonographic measurement of optic nerve sheath diameter ▶     Li-juan Wang, Yan Yao, Liang-shu Feng et al.     We aimed to quantitatively assess intracranial pressure (ICP) using optic nerve sheath diameter (ONSD) measurements. We recruited 316 neurology patients in whom ultrasonographic …             New murine Niemann-Pick type C models bearing a pseudoexon-generating mutation recapitulate the main neurobehavioural and molecular features of the disease ▶     Marta Gómez-Grau, Júlia Albaigès, Josefina Casas et al.     Niemann-Pick disease type C (NPC) is a rare neurovisceral disease caused mainly by mutations in the NPC1 gene. This autosomal recessive lysosomal disorder is characterised by the …             Bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells ▶     P. S. Saneesh Babu, Prasad M. Manu, T. Jayaram Dhanya et al.     Photodynamic therapy (PDT) is a clinically established and highly evolving treatment modality for cancer. 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