EMBO reports Table of Contents for December 2016; Vol. 17, No. 12

December 2016 | Volume 17, Number 12 Submit Table of Contents Opinion News & Views Science & Society Reviews Scientific Reports Articles Corrigendum Volume 17, Number 12 Opinion From HDACi to KDACi: we need to revisit non‐epigenetic pathways affected by inhibiting lysine deacetylases in therapy Lysine deacetylases act on many other proteins apart from histones, with effects on metabolism and gene expression. We need to be cautious that therapeutically used KDACi have a much more widespread impact than anticipated. Axel Imhof and Shahaf Peleg Published online 24.10.2016 News & Views Neurons acetylate their way to migration A study in this issue shows that CAMDI loss leads to cellular and behavioural phenotypes that model autism spectrum disorders and which can be ameliorated by HDAC6 inhibition. Sarah Catherine Borrie and Claudia Bagni Published online 21.10.2016 Plants get on PAR with poly(ADP‐ribosyl)ation A study in this issue identifies the first set of poly(ADP‐ribosyl)ated plant proteins and shows that in vivo poly(ADP‐ribosyl)ation of one of these proteins is important for its role in plant immunity. Frank LH Menke Published online 02.11.2016 Science & Society Una destinatio, viae diversae: Does exposure to the vaginal microbiota confer health benefits to the infant, and does lack of exposure confer disease risk? There are lingering concerns that Cesarean surgery could impede on the child's health due to a lack of exposure to the mother's vaginal microbiota. However, the evidence so far is not sufficient to affirm whether the mode of birth influences disease risk or whether it is other causes. Kjersti Aagaard, Christopher J Stewart, and Derrick Chu Published online 24.11.2016 Reframing the “Cancer Moonshot”: How experts and non‐experts interpret the problem of cancer The reactions to an Op‐Ed that challenged US President Obama's new initiative to find a cure for cancer represent a rich material of how experts and lay people understand and frame the disease. Jarle Breivik Published online 04.11.2016 Euthanizing zebrafish legally in Europe: Are the approved methods of euthanizing zebrafish appropriate to research reality and animal welfare? As zebrafish are becoming an increasingly important model organism in biomedical research, the approved methods of euthanizing fish should be expanded to improve both animal welfare and the quality of research. Ana M Valentim, Fredericus J van Eeden, Uwe Strähle, and I Anna S Olsson Published online 18.10.2016 Piecing together a different picture: A host of new studies on autism have begun decoding the longstanding puzzle of its causes Multi‐disciplinary research into the causes of autism reveals how a complex interplay of genes and diverse environmental factors can affect neuronal development during early childhood. Andrea Rinaldi Published online 17.11.2016 Plant microbiomes and sustainable agriculture: Deciphering the plant microbiome and its role in nutrient supply and plant immunity has great potential to reduce the use of fertilizers and biocides in agriculture Plant microbiota play a crucial role by helping their host plant extract nutrients and defend against pathogens. Understanding these mutually beneficial relationships can greatly improve sustainable agriculture. Philip Hunter Published online 24.10.2016 Reviews Molecular mechanisms of asymmetric divisions in mammary stem cells During development and regeneration, stem cells balance symmetric and asymmetric cell divisions (ACDs) in response to intrinsic cues and extracellular signals. This review focuses on the molecular mechanisms of ACDs in mammary stem cells, and their role in breast cancer development. Angela Santoro, Thalia Vlachou, Manuel Carminati, Pier Giuseppe Pelicci, and Marina Mapelli Published online 21.11.2016 PKM2, cancer metabolism, and the road ahead Isoforms of the glycolytic enzyme pyruvate kinase support tumor metabolism and growth. This review discusses PKM2 studies that have elucidated both normal and cancer cell metabolism. Talya L Dayton, Tyler Jacks, and Matthew G Vander Heiden Published online 17.11.2016 Scientific Reports Alternative lengthening of human telomeres is a conservative DNA replication process with features of break‐induced replication Human cells that rely on ALT to maintain telomere length use break‐induced replication, a DNA repair pathway associated with conservative rather than semiconservative DNA replication. Fani‐Marlen Roumelioti, Sotirios K Sotiriou, Vasiliki Katsini, Maria Chiourea, Thanos D Halazonetis, and Sarantis Gagos Published online 19.10.2016 Central CCL2 signaling onto MCH neurons mediates metabolic and behavioral adaptation to inflammation CCL2/CCR2 signaling is a central relay for the behavioral and metabolic changes associated with sickness behavior in response to inflammation. Enhanced CCL2/CCR2 signaling inhibits the activity of MCH neurons in the hypothalamus and induces a decrease in body weight and food intake. Ophélia Le Thuc, Céline Cansell, Miled Bourourou, Raphaël GP Denis, Katharina Stobbe, Nadège Devaux, Alice Guyon, Julie Cazareth, Catherine Heurteaux, William Rostène, Serge Luquet, Nicolas Blondeau, Jean‐Louis Nahon, and Carole Rovère Published online 12.10.2016 Rab22a controls MHC‐I intracellular trafficking and antigen cross‐presentation by dendritic cells Cross‐presentation by MHC class I molecules allows the detection of exogenous antigens and is crucial to initiate cytotoxic immune responses against many pathogens. This report identifies the small GTPase Rab22a as a key regulator of MHC‐I trafficking and antigen cross‐presentation in dendritic cells. Ignacio Cebrian, Cristina Croce, Néstor A Guerrero, Nicolas Blanchard, and Luis S Mayorga Published online 10.10.2016 Zika virus inhibits type‐I interferon production and downstream signaling Zika virus (ZIKV) is a mosquito‐borne pathogen that causes Guillain–Barré syndrome in adults and microcephaly in newborns. This report shows that ZIKV infection inhibits the induction of type‐I interferons by downregulating IRF3 and antiviral NF‐κB‐mediated signaling and targets STAT2 for proteasomal degradation. Anil Kumar, Shangmei Hou, Adriana M Airo, Daniel Limonta, Valeria Mancinelli, William Branton, Christopher Power, and Tom C Hobman Published online 24.10.2016 Molecular insights into protein synthesis with proline residues Proline impedes the rate of peptide bond formation or induces ribosome stalling. Structures of the eukaryotic ribosome bound to prolyl‐ and diprolyl‐tRNA analogs reveal a unique binding mode of proline and how this impacts peptide synthesis. Sergey Melnikov, Justine Mailliot, Lukas Rigger, Sandro Neuner, Byung‐Sik Shin, Gulnara Yusupova, Thomas E Dever, Ronald Micura, and Marat Yusupov Published online 08.11.2016 Articles Rescue of CAMDI deletion‐induced delayed radial migration and psychiatric behaviors by HDAC6 inhibitor The HDAC6‐specific inhibitor Tubastatin A rescues delayed neuronal migration and psychiatric behaviors of CAMDI‐deficient mice. The study provides new insight into the pathogenesis of psychiatric disorders with potential therapeutic implications. Toshifumi Fukuda, Shun Nagashima, Takaya Abe, Hiroshi Kiyonari, Ryoko Inatome, and Shigeru Yanagi Published online 13.10.2016 PARylation of the forkhead‐associated domain protein DAWDLE regulates plant immunity Protein poly(ADP‐ribosyl)ation plays crucial roles in various cellular processes, but few PARylation targets have been identified so far in plants. This study reports on a proteome‐wide screen for PARP targets in Arabidopsis and identifies PARylation of DAWDLE as essential for its function in plant immunity. Baomin Feng, Shisong Ma, Sixue Chen, Ning Zhu, Shuxin Zhang, Bin Yu, Yu Yu, Brandon Le, Xuemei Chen, Savithramma P Dinesh‐Kumar, Libo Shan, and Ping He Published online 18.10.2016 Identification of novel genes and networks governing hematopoietic stem cell development In this study, the authors use a genome‐wide RNAi silencing approach to find stage‐specific gene networks required for HSPC development. A novel conserved group of genes is identified to be required for HSPC specification in zebrafish and the maintenance of human HSCs. Tianxu Han, Chao‐Shun Yang, Kung‐Yen Chang, Danhua Zhang, Farhad B Imam, and Tariq M Rana Published online 26.10.2016 Loss of Nat4 and its associated histone H4 N‐terminal acetylation mediates calorie restriction‐induced longevity Histone H4 N‐terminal acetylation (N‐acH4) catalyzed by Nat4 links calorie restriction and a specific transcriptional program that impacts on cellular lifespan. Diego Molina‐Serrano, Vassia Schiza, Christis Demosthenous, Emmanouil Stavrou, Jan Oppelt, Dimitris Kyriakou, Wei Liu, Gertrude Zisser, Helmut Bergler, Weiwei Dang, and Antonis Kirmizis Published online 31.10.2016 The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i‐AAA protease YME1L The membrane scaffold SLP2 anchors a large protease complex containing the rhomboid protease PARL and the i‐AAA protease YME1L in the inner membrane of mitochondria, termed the SPY complex. Assembly into the SPY complex modulates PARL activity toward its substrate proteins PINK1 and PGAM5. Timothy Wai, Shotaro Saita, Hendrik Nolte, Sebastian Müller, Tim König, Ricarda Richter‐Dennerlein, Hans‐Georg Sprenger, Joaquin Madrenas, Mareike Mühlmeister, Ulrich Brandt, Marcus Krüger, and Thomas Langer Published online 13.10.2016 Crystal structure of Mdm12 reveals the architecture and dynamic organization of the ERMES complex The crystal structure of Mdm12, a cytosolic component of the ER–mitochondria encounter structure (ERMES) complex, reveals the structural basis for phospholipid binding and selectivity of Mdm12, and the organization of SMP domains in the ERMES complex. Hanbin Jeong, Jumi Park, and Changwook Lee Published online 07.11.2016 Resetting cancer stem cell regulatory nodes upon MYC inhibition Cancer stemlike cells are key to cancer development and therapy. The MYC‐interfering polypeptide, Omomyc, impairs the carcinogenic potential of human glioblastoma stemlike cells (GSCs) and affects proper MYC genomic localization. This indicates that the gene regulatory nodes determining GSC identity are MYC dependent. Silvia Galardi, Mauro Savino, Fiorella Scagnoli, Serena Pellegatta, Federica Pisati, Federico Zambelli, Barbara Illi, Daniela Annibali, Sara Beji, Elisa Orecchini, Maria Adele Alberelli, Clara Apicella, Rosaria Anna Fontanella, Alessandro Michienzi, Gaetano Finocchiaro, Maria Giulia Farace, Giulio Pavesi, Silvia Anna Ciafrè, and Sergio Nasi Published online 16.11.2016 Phosphocysteine in the PRL‐CNNM pathway mediates magnesium homeostasis The PRL‐CNNM pathway regulates Mg2+ homeostasis and tumor progression. This study shows that the formation of a catalytic phosphocysteine PRL intermediate regulates binding to CNNM magnesium transporters and Mg2+ efflux. Irina Gulerez, Yosuke Funato, Howie Wu, Meng Yang, Guennadi Kozlov, Hiroaki Miki, and Kalle Gehring Published online 17.11.2016 Corrigendum Sulfatase activities are regulated by the interaction of the sulfatase‐modifying factor 1 with SUMF2 Ester Zito, Alessandro Fraldi, Stefano Pepe, Ida Annunziata, Gary Kobinger, Paola Di Natale, Andrea Ballabio, and Maria Pia Cosma Published online 01.12.2016   Unsubscribe from or edit your subscription for this service. Or by mail: Customer Service * 425 Broadway St * Redwood City, CA 94063 * U.S.A. Copyright © 2016 by the European Molecular Biology Organisation.