The EMBO Journal Table of Contents for 15 April 2015; Vol. 34, No. 8

15 April 2015 | Volume 34, Number 8 Submit Table of Contents Have you seen? Review Articles Volume 34, Number 8 Have you seen? Human germline differentiation charts a new course Sperm and egg derive from germline cells. Two studies report efficient generation of human germline cells in vitro, implicating PRDM1 and SOX17 in the process. This is a step towards in vitro gametogenesis and may allow screening of agents affecting fertility. Di Chen and Amander T Clark Published online 18.03.2015 Unsaturated fatty acid‐induced non‐canonical autophagy: unusual? or unappreciated? Saturated and unsaturated fatty acids induce canonical autophagy and a novel non‐canonical autophagic pathway, respectively; this might represent an ancient mechanism for the recycling of lipophiles from membranes via distinct degradative pathways. Vytas A Bankaitis Published online 11.03.2015 Interrupting synoviolin play at the ER: a plausible action to elevate mitochondrial energetics and silence obesity Inhibiting the E3 ligase synoviolin with the specific inhibitor LS‐102 can be potentially exploited to fight obesity, as this ligase is now shown to control body weight and energy expenditure in mice. Meghan S Soustek and Pere Puigserver Published online 03.03.2015 Presynapses go nuclear! Synaptic signaling altering nuclear gene expression and long‐term changes in synapse function occurs not only on postsynaptic, but also on presynaptic sites. Dana O Kravchick and Bryen A Jordan Published online 11.03.2015 Review Focus: Induced Pluripotency & Cellular Reprogramming: Application of biomaterials to advance induced pluripotent stem cell research and therapy Karp and colleagues critically review state‐of‐the‐art biomaterials essential to fully exploit the translational potential of the iPS technologies. Zhixiang Tong, Aniruddh Solanki, Allison Hamilos, Oren Levy, Kendall Wen, Xiaolei Yin, and Jeffrey M Karp Published online 12.03.2015 Articles Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile Primordial germ cells (PGCs) generate sperm and oocytes. A defined differentiation system induces human pre‐migratory PGC‐like cells (PGCLCs) from pluripotent stem cells (PSCs) in vitro. This protocol allows disease modeling and may lead to the generation of gametes for reproductive medicine. Fumihiro Sugawa, Marcos J Araúzo‐Bravo, Juyong Yoon, Kee‐Pyo Kim, Shinya Aramaki, Guangming Wu, Martin Stehling, Olympia E Psathaki, Karin Hübner, and Hans R Schöler Published online 06.03.2015 Unsaturated fatty acids induce non‐canonical autophagy A systematic screen in cancer cells reveals that unsaturated and saturated fatty acids induce autophagy via distinct pathways, with unsaturated fatty acids acting in a Golgi‐dependent but Beclin‐1‐independent manner. Mireia Niso‐Santano, Shoaib Ahmad Malik, Federico Pietrocola, José Manuel Bravo‐San Pedro, Guillermo Mariño, Valentina Cianfanelli, Amena Ben‐Younès, Rodrigo Troncoso, Maria Markaki, Valentina Sica, Valentina Izzo, Kariman Chaba, Chantal Bauvy, Nicolas Dupont, Oliver Kepp, Patrick Rockenfeller, Heimo Wolinski, Frank Madeo, Sergio Lavandero, Patrice Codogno, Francis Harper, Gérard Pierron, Nektarios Tavernarakis, Francesco Cecconi, Maria Chiara Maiuri, Lorenzo Galluzzi, and Guido Kroemer Published online 13.01.2015 The E3 ligase synoviolin controls body weight and mitochondrial biogenesis through negative regulation of PGC‐1β Selective inhibition or knockout of ER‐resident E3 ubiquitin ligase SYVN1 increases PGC‐1β activity and basal energy expenditure, preventing weight gain in a mouse model for obesity. Hidetoshi Fujita, Naoko Yagishita, Satoko Aratani, Tomoko Saito‐Fujita, Saori Morota, Yoshihisa Yamano, Magnus J Hansson, Masato Inazu, Hiroko Kokuba, Katsuko Sudo, Eiichi Sato, Ko‐ichi Kawahara, Fukami Nakajima, Daisuke Hasegawa, Itsuro Higuchi, Tomoo Sato, Natsumi Araya, Chie Usui, Kenya Nishioka, Yu Nakatani, Ikuro Maruyama, Masahiko Usui, Naomi Hara, Hiroyuki Uchino, Eskil Elmer, Kusuki Nishioka, and Toshihiro Nakajima Published online 19.02.2015 Synaptic activity controls localization and function of CtBP1 via binding to Bassoon and Piccolo Synapto‐nuclear shuttling of the transcriptional co‐repressor CtBP1 is regulated by neuronal activity‐modulated NAD/NADH balance, and serves to couple synaptic activity with gene expression. Daniela Ivanova, Anika Dirks, Carolina Montenegro‐Venegas, Cornelia Schöne, Wilko D Altrock, Claudia Marini, Renato Frischknecht, Denny Schanze, Martin Zenker, Eckart D Gundelfinger, and Anna Fejtova Published online 04.02.2015 Cyclin O (Ccno) functions during deuterosome‐mediated centriole amplification of multiciliated cells Cyclin O mutations cause congenital mucociliary clearance disorder linked to a reduced number of motile cilia in the airway epithelium. This is caused by malfunctioning deuterosomes that lead to fewer and partially defective centrioles. Maja C Funk, Agata N Bera, Tabea Menchen, Georg Kuales, Kerstin Thriene, Soeren S Lienkamp, Jörn Dengjel, Heymut Omran, Marcus Frank, and Sebastian J Arnold Published online 23.02.2015 Lysyl oxidase‐like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression In vivo genetic analysis of LOXL2 evidences fundamental roles during mouse development. Importantly, mice challenged by a chemical skin carcinogenesis protocol uncover a role for LOXL2 in NOTCH1 inhibition for carcinoma progression. Alberto Martin, Fernando Salvador, Gema Moreno‐Bueno, Alfredo Floristán, Cristina Ruiz‐Herguido, Eva P Cuevas, Saleta Morales, Vanesa Santos, Katalin Csiszar, Pierre Dubus, Jody J Haigh, Anna Bigas, Francisco Portillo, and Amparo Cano Published online 10.03.2015 SIRT3‐dependent GOT2 acetylation status affects the malate–aspartate NADH shuttle activity and pancreatic tumor growth Acetylation of oxaloacetate transaminase (GOT2) promotes its binding with malate dehydrogenase (MDH2), thereby regulating the malate–aspartate shuttle activity, cytosol‐to‐mitochondrion transfer of NADH, oxidative protection and tumor growth. Hui Yang, Lisha Zhou, Qian Shi, Yuzheng Zhao, Huaipeng Lin, Mengli Zhang, Shimin Zhao, Yi Yang, Zhi‐Qiang Ling, Kun‐Liang Guan, Yue Xiong, and Dan Ye Published online 09.03.2015 Structural basis for a novel mechanism of DNA bridging and alignment in eukaryotic DSB DNA repair Eukaryotic PolX family DNA polymerases mediate DNA double‐stranded break repair and V(D)J recombination (TdT). The X‐ray structure of TdT bound to an annealed DNA break reveals the mechanism of the unique ability of PolX enzymes to bridge DNA ends. Jérôme Gouge, Sandrine Rosario, Félix Romain, Frédéric Poitevin, Pierre Béguin, and Marc Delarue Published online 11.03.2015   Unsubscribe from or edit your subscription for this service. Or by mail: Customer Service * 425 Broadway St * Redwood City, CA 94063 * U.S.A. Copyright © 2015 by the European Molecular Biology Organisation.