Nature Cell Biology contents: June 2012 Volume 14 Number 6, pp 557 - 649

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See it online only at Duolink-vs-CoIP.com Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement The EMBO Meeting 2012 22-25 September in Nice, France Keynotes: Paul Nurse & Linda Partridge 20 concurrent sessions covering the life sciences Speakers include: Kari Alitalo, Karl Deisseroth, Steven Henikoff, Ruth Lehmann, Dirk Schübeler & Rob Singer Abstract submission & early registration: 12 June 2012 www.the-embo-meeting.org   Advertisement READ and INCUBATE CELLS in parallel Tecan's NEW CO2/O2 Gas Control Module for Infinite 200 PRO! • Consistent physiological conditions • Simultaneous CO2 and O2 • No transport of cell cultures from incubator to reader • Complete data sets overnight Learn more   Editorial Top Stem cell research: Regulating translational application p557 doi:10.1038/ncb2517 As the pace of translational stem cell research accelerates, researchers and governing bodies must work together to develop and implement rigorous ethical standards to guide the transition into the clinical sphere. Full Text | PDF Turning Points Top Redefining differentiation: Reshaping our ends p558 Helen M. Blau doi:10.1038/ncb2506 Full Text | PDF News and Views Top T time for point centromeres pp559 - 561 Karen E. Gascoigne and Iain M. Cheeseman doi:10.1038/ncb2509 The diverse nature of eukaryotic centromere structure has led to a prevailing view that the kinetochore-chromatin interface is fundamentally different in distinct species. Two studies now challenge this dogma with the identification of budding yeast homologues of the vertebrate centromere DNA-binding proteins CENP-T and CENP-W. Full Text | PDF See also: Article by Schleiffer et al. | Article by Bock et al. Cdt1 throws kinetochore-microtubule attachments for a loop pp561 - 563 Daniel R. Matson and P. Todd Stukenberg doi:10.1038/ncb2513 The Ndc80 complex links spindle microtubules to the kinetochore to ensure the proper segregation of chromosomes during mitosis. Analysis of the replication licensing factor Cdt1 during mitosis now reveals a cooperative role with the Ndc80 complex in establishing stable microtubule attachments to the spindle. Full Text | PDF See also: Article by Varma et al. Mcl-1 rescues a glitch in the matrix pp563 - 565 Joshua L. Andersen and Sally Kornbluth doi:10.1038/ncb2511 Bcl-2 family proteins are known to control cell death and influence mitochondrial function. The function of Mcl-1, an anti-apoptotic Bcl-2 protein, is now shown to depend on its subcellular localization. Mcl-1 at the mitochondrial outer membrane inhibits mitochondrial permeabilization to block apoptosis. However, a cleaved form of Mcl-1 localizes to the mitochondrial matrix and controls inner mitochondrial morphology and oxidative phosphorylation, without directly modulating apoptosis. Full Text | PDF See also: Article by Perciavalle et al. Research Highlights Visualizing clathrin-mediated endocytosis | Pushing a way to axis determination | Trim39 ligase keeps apoptosis going | How to EB-ind microtubule ends Cell Biology JOBS of the week Postdoc position in Network and Stochasic Dynamics in Biology Department of Physics, University of Tennessee, Knoxville Ph.D. student in Bioinformatics (Eco-Systems Biology) University of Luxembourg / LCSB Post-doctoral position in chromosome biology CEA Lecturers in Biochemistry, Plant Biology, Bioinformatics or Marine Biology (Education and Research) University of Exeter Open Post doctoral position to work on Cell nanomechanics Bionanomechanics Lab (Spanish National Research Council) More Science jobs from Cell Biology EVENT 10th International Congress on Cell Biology 25.-28.07.12 Brazil More science events from Articles Top MYC suppresses cancer metastasis by direct transcriptional silencing of αv and β3 integrin subunits pp567 - 574 Hong Liu, Derek C. Radisky, Dun Yang, Ren Xu, Evette S. Radisky, Mina J. Bissell and J. Michael Bishop doi:10.1038/ncb2491 Radisky and colleagues show that, in contrast to its pro-tumorigenic properties, the MYC oncogene is also able to inhibit metastasis by suppressing cell migration and invasiveness. Mechanistically, they show that MYC transcriptionally represses the integrin αv and β3 subunits, which are needed for efficient cell motility and invasion. Abstract | Full Text | PDF Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration pp575 - 583 Rhonda M. Perciavalle, Daniel P. Stewart, Brian Koss, John Lynch, Sandra Milasta, Madhavi Bathina, Jamshid Temirov, Megan M. Cleland, Stéphane Pelletier, John D. Schuetz, Richard J. Youle, Douglas R. Green and Joseph T. Opferman doi:10.1038/ncb2488 MCL-1 is an anti-apoptotic BCL-2 family member and is frequently upregulated in cancer, but the mechanism by which it promotes cell survival has been elusive. Opferman and colleagues provide insight into this process by showing that MCL-1 exists in different forms with discrete localizations and functions. MCL-1 variants targeted to the outer mitochondrial membrane antagonize BAX and BAK activation, whereas an N-terminally truncated isoform localizes to the mitochondrial matrix and regulates mitochondrial metabolism. Abstract | Full Text | PDF See also: News and Views by Andersen & Kornbluth Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail pp584 - 592 Ralph Thomas Böttcher, Christopher Stremmel, Alexander Meves, Hannelore Meyer, Moritz Widmaier, Hui-Yuan Tseng and Reinhard Fässler doi:10.1038/ncb2501 Integrin internalization through the endosomal pathway can lead either to recycling back to the surface or to lysosomal degradation. Faessler and colleagues now show that, following internalization, β1 integrins are bound by sorting nexin 17 in early endosomes to prevent integrin degradation in lysosomes and to promote surface recycling. Abstract | Full Text | PDF Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment pp593 - 603 Dileep Varma, Srikripa Chandrasekaran, Lynsie J. R. Sundin, Karen T. Reidy, Xiaohu Wan, Dawn A. D. Chasse, Kathleen R. Nevis, Jennifer G. DeLuca, E. D. Salmon and Jeanette Gowen Cook doi:10.1038/ncb2489 The replication origin licensing factor Cdt1 is now demonstrated to function at the kinetochore in mitosis. Cook, Salmon and colleagues show that Cdt1 binds to the loop domain of Hec1 in the Ndc80 kinetochore complex and stabilizes kinetochore-microtubule attachment. Abstract | Full Text | PDF See also: News and Views by Matson & Stukenberg CENP-T proteins are conserved centromere receptors of the Ndc80 complex pp604 - 613 Alexander Schleiffer, Michael Maier, Gabriele Litos, Fabienne Lampert, Peter Hornung, Karl Mechtler and Stefan Westermann doi:10.1038/ncb2493 Multi-protein kinetochore complexes bind to the centromeric region of chromosomes to ensure accurate spindle attachment and chromosome segregation, although centromere organization differs widely between species. Westermann and colleagues now identify the budding yeast protein Cnn1 as the orthologue of mammalian CENP-T. They show that it binds to the Ndc80 kinetochore complex and functions in chromosome segregation, illustrating a conserved role for this protein. Abstract | Full Text | PDF See also: News and Views by Gascoigne & Cheeseman Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore pp614 - 624 Lucy J. Bock, Cinzia Pagliuca, Norihiko Kobayashi, Ryan A. Grove, Yusuke Oku, Kriti Shrestha, Claudio Alfieri, Cristina Golfieri, Amanda Oldani, Marianna Dal Maschio, Rodrigo Bermejo, Tony R. Hazbun, Tomoyuki U. Tanaka and Peter De Wulf doi:10.1038/ncb2495 The kinetochore is a multiprotein complex that tethers chromosomes to the mitotic spindle for accurate chromosome segregation. De Wulf and colleagues now show in budding yeast that the protein Cnn1 functions at the kinetochore and is recruited to the inner kinetochore, in a manner dependent on its phosphorylation mediated by the Cdc28, Mps1 and Ipl1 kinases. Abstract | Full Text | PDF See also: News and Views by Gascoigne & Cheeseman TRADD contributes to tumour suppression by regulating ULF-dependent p19Arf ubiquitylation pp625 - 633 Iok In Christine Chio, Masato Sasaki, Danny Ghazarian, Juan Moreno, Susan Done, Takeshi Ueda, Satoshi Inoue, Yu-Ling Chang, Nien Jung Chen and Tak Wah Mak doi:10.1038/ncb2496 Mak and colleagues report that TRADD, an adaptor protein important in tumour necrosis factor receptor (TNFR) signalling, contributes to tumour suppression independently of TNFR. They show that nuclear TRADD binds to the tumour suppressor p19Arf and inhibits its interaction with the E3 ubiquitin ligase ULF, leading to p19Arf stability and promoting cell senescence. Abstract | Full Text | PDF Letter Top Reconstitution of clathrin-coated bud and vesicle formation with minimal components pp634 - 639 Philip N. Dannhauser and Ernst J. Ungewickell doi:10.1038/ncb2478 Clathrin-mediated endocytosis requires the generation of membrane curvature and subsequent formation of clathrin-coated vesicles. Using a cell-free system, Dannhauser and Ungewickell show that clathrin polymerization is capable of inducing membrane deformation and generating spherical vesicles. First paragraph | Full Text | PDF Resource Top Patchwork organization of the yeast plasma membrane into numerous coexisting domains pp640 - 648 Felix Spira, Nikola S. Mueller, Gisela Beck, Philipp von Olshausen, Joachim Beig and Roland Wedlich-Söldner doi:10.1038/ncb2487 Wedlich-Soldner and colleagues characterize the localization of plasma membrane proteins in Saccharomyces cerevisiae by total internal reflection microscopy and deconvolution. Their data reveal a self-organization of proteins into patterns and demonstrate that association of the arginine transporter Can1 with its membrane domain is important for its function. Abstract | Full Text | PDF Corrigendum Top Opposing roles for p16Ink4a and p19Arf in senescence and ageing caused by BubR1 insufficiency p649 Darren J. Baker, Carmen Perez-Terzic, Fang Jin, Kevin S. Pitel, Nicolas J. Niederländer, Karthik Jeganathan, Satsuki Yamada, Santiago Reyes, Lois Rowe, H. Jay Hiddinga, Norman L. Eberhardt, Andre Terzic and Jan M. van Deursen doi:10.1038/ncb2519 Full Text | PDF Erratum Top Kdm2b promotes induced pluripotent stem cell generation by facilitating gene activation early in reprogramming p649 Gaoyang Liang, Jin He and Yi Zhang doi:10.1038/ncb2518 Full Text | PDF Top Advertisement Nature Outlook: Diabetes 5% of the world's population are afflicted by either type 1 diabetes, an autoimmune disorder, or type 2 diabetes, largely linked to lifestyle. 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