The EMBO Journal - Table of Contents alert Volume 31 Issue 11

TABLE OF CONTENTS 30 May 2012 | Volume 31, Issue 11 Have you seen? Review Articles Corrigendum Also new AOP Advertisement Your next best friend in epigenetics Diagenode's IP-Star instrument completely automates your ChIP and methylated DNA immunoprecipitation assays. Eliminate the challenges of optimizing your epigenetics assay with the IP-Star. Achieve higher consistency, increased productivity, and higher quality data. Learn more about affordable epigenetics workflow automation with the IP-Star System. Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement THREE DECADES OF WNT SIGNALLING Read The EMBO Journal special focus issue to mark the 30th anniversary of the discovery of the first mammalian Wnt-gene. Roel Nusse and Harold Varmus survey the past, present and future of Wnt research. Hans Clevers, Paul Polakis, Christof Niehrs, Konrad Basler and Kang Sheng provide authoritative overviews of a burgeoning field that unites developmental biology, signalling and cancer research.   Have you seen? Top SUMO unloads the Kap114 cab Translocation of proteins across the nuclear pore complex depends on transport receptors and the GTPase Ran. SUMO conjugation of an importin β-like transport receptor by the conjugating enzyme Mms21 stimulates the intranuclear release of cargo from the transport protein karyopherin. Andreas Werner and Frauke Melchior The EMBO Journal (2012), 31, 2439 - 2440; 10.1038/emboj.2012.103 Abstract | Full text | PDF Published online: 04 May 2012 Intestinal stem cells: no longer immortal but ever so clever…. Intestinal stem cells in both flies and mice show remarkable plasticity by employing symmetric stem cell divisions in response to environmental demands. Bruce A Edgar The EMBO Journal (2012), 31, 2441 - 2443; 10.1038/emboj.2012.133 Abstract | Full text | PDF Published online: 11 May 2012 A new tumour suppressor enters the network of intestinal progenitor cell homeostasis Nuclear Receptor Binding Protein 1 (NRBP1) enters the stage as a novel tumour suppressor that impinges on intestinal progenitor homeostasis by regulating Sall4 protein level. Ee Hong Tan and Owen James Sansom The EMBO Journal (2012), 31, 2444 - 2445; 10.1038/emboj.2012.115 Abstract | Full text | PDF Published online: 17 April 2012 Not lost in translation: stepwise regulation of microRNA targets Genome-wide ribosome footprinting and RNA sequencing on miR-430 mRNA targets in zebrafish embryos reveal distinct insights into translational repression versus mRNA decay. Maja M Janas and Carl D Novina The EMBO Journal (2012), 31, 2446 - 2447; 10.1038/emboj.2012.119 Abstract | Full text | PDF Published online: 20 April 2012 Review Top The updated biology of hypoxia-inducible factor This review summarizes and structures recent discoveries on biological functions mediated by hypoxia-inducible factor-1. Samantha N Greer, Julie L Metcalf, Yi Wang and Michael Ohh The EMBO Journal (2012), 31, 2448 - 2460; 10.1038/emboj.2012.125 Abstract | Full text | PDF Published online: 04 May 2012 Articles Top Sumoylation regulates Kap114-mediated nuclear transport Nucleocytoplasmic transport is mediated by importin [beta]/karyopherin transport receptors and regulated by the GTPase cycle of Ran/Gsp1. SUMOylation of the yeast karyopherin Kap114 stimulates Ran/Gsp1-dependent cargo release in the nucleus. Ute Rothenbusch, Marc Sawatzki, Yiming Chang, Stefanie Caesar and Gabriel Schlenstedt The EMBO Journal (2012), 31, 2461 - 2472; 10.1038/emboj.2012.102 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 04 May 2012 Drosophila midgut homeostasis involves neutral competition between symmetrically dividing intestinal stem cells Quantitative analyses on clonal fate of Drosophila midgut intestinal stem cells suggest that neutral competition of symmetrically dividing cells contributes to physiological tissue dynamics. The study thus adds to current models on stem-cell behaviour in flies and vertebrates. Joaquín de Navascués, Carolina N Perdigoto, Yu Bian, Markus H Schneider, Allison J Bardin, Alfonso Martínez-Arias and Benjamin D Simons The EMBO Journal (2012), 31, 2473 - 2485; 10.1038/emboj.2012.106 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 20 April 2012 Nuclear receptor binding protein 1 regulates intestinal progenitor cell homeostasis and tumour formation Arising from a ras-interaction screen in C. elegans, nuclear receptor binding protein 1 (NRBP1) is shown to impose a crypt progenitor phenotype in mice and is proposed as a novel tumour suppressor in human cancer. Catherine H Wilson, Catriona Crombie, Louise van der Weyden, George Poulogiannis, Alistair G Rust, Mercedes Pardo, Tannia Gracia, Lu Yu, Jyoti Choudhary, Gino B Poulin, Rebecca E McIntyre, Douglas J Winton, H Nikki March, Mark J Arends, Andrew G Fraser and David J Adams The EMBO Journal (2012), 31, 2486 - 2497; 10.1038/emboj.2012.91 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 17 April 2012 Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion Employing a conditionally inactive gene trap allele, Cdk7's function in regulating cellular proliferation by Cdk1/2-phosphorylation is convincingly dissected from alternative notions on CTD-phosphorylation of RNA Pol II. Premature aging phenotypes caused by stem cell depletion lend the necessary functional support. Miguel Ganuza, Cristina Sáiz-Ladera, Marta Cañamero, Gonzalo Gómez, Ralph Schneider, María A Blasco, David Pisano, Jesús M Paramio, David Santamaría and Mariano Barbacid The EMBO Journal (2012), 31, 2498 - 2510; 10.1038/emboj.2012.94 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 April 2012 A new non-catalytic role for ubiquitin ligase RNF8 in unfolding higher-order chromatin structure RNF8 and RNF168 cooperate during DNA repair to ubiquitylate histone H2A. Strikingly, RNF8 also exhibits a chromatin decondensation activity, which is independent of its ubiquitin-ligase activity and RNF168, but involves a NuRD subunit and which allows assembly of repair factors at DNA lesions. Martijn S Luijsterburg, Klara Acs, Leena Ackermann, Wouter W Wiegant, Simon Bekker-Jensen, Dorthe H Larsen, Kum Kum Khanna, Haico van Attikum, Niels Mailand and Nico P Dantuma The EMBO Journal (2012), 31, 2511 - 2527; 10.1038/emboj.2012.104 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 24 April 2012 Large conformational changes in MutS during DNA scanning, mismatch recognition and repair signalling New intermediates in the nucleotide-dependent transition from mismatch recognition to repair-initiating sliding clamp are revealed via single-molecule FRET of the key mismatch repair protein MutS. Ruoyi Qiu, Vanessa C DeRocco, Credle Harris, Anushi Sharma, Manju M Hingorani, Dorothy A Erie and Keith R Weninger The EMBO Journal (2012), 31, 2528 - 2540; 10.1038/emboj.2012.95 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 April 2012 Structure of a dominant-negative helix-loop-helix transcriptional regulator suggests mechanisms of autoinhibition The first structural views of an Id-like negative transcription factor, HMM, provide insights into HMM control of, and specificity for, its cognate bHLH transcription factor Olig1. Ryohei Ishii, Kazunobu Isogaya, Azusa Seto, Daizo Koinuma, Yuji Watanabe, Fumio Arisaka, So-ichi Yaguchi, Hiroaki Ikushima, Naoshi Dohmae, Kohei Miyazono, Keiji Miyazawa, Ryuichiro Ishitani and Osamu Nureki The EMBO Journal (2012), 31, 2541 - 2552; 10.1038/emboj.2012.77 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 27 March 2012 Differential effects of viral silencing suppressors on siRNA and miRNA loading support the existence of two distinct cellular pools of ARGONAUTE1 Plant viruses encode suppressors (VSRs) that target the host antiviral RNA silencing pathway. Here, VSRs are shown to differentially impair siRNA and miRNA binding to Argonaute1, AGO1, revealing distinct AGO1 pools that are preferentially loaded with either siRNAs or miRNAs. Gregory Schott, Arturo Mari-Ordonez, Christophe Himber, Abdelmalek Alioua, Olivier Voinnet and Patrice Dunoyer The EMBO Journal (2012), 31, 2553 - 2565; 10.1038/emboj.2012.92 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 24 April 2012 Co-transcriptional degradation of aberrant pre-mRNA by Xrn2 The nuclear pre-mRNA surveillance and degradation pathways in human cells are not well understood. The 5–3′ exonuclease Xrn2 plays a role in co-transcriptional degradation of mRNAs that have defects in splicing or 3′ processing. Lee Davidson, Alastair Kerr and Steven West The EMBO Journal (2012), 31, 2566 - 2578; 10.1038/emboj.2012.101 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 20 April 2012 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay Nonfunctional ribosomes and rRNAs elicit diverse quality control mechanisms. 25S rRNA decay is initiated by the ubiquitination of the nonfunctional 60S subunit and its Cdc48-dependent dissociation from the intact 40S subunit, followed by proteasomal protein degradation and finally 25S rRNA degradation. Kotaro Fujii, Makoto Kitabatake, Tomoko Sakata and Mutsuhito Ohno The EMBO Journal (2012), 31, 2579 - 2589; 10.1038/emboj.2012.85 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 April 2012 Structural basis for Arf6–MKLP1 complex formation on the Flemming body responsible for cytokinesis The GTPase ARF6, found to localize to the cleavage furrow during mammalian cell division, functions in cytokinesis by recruiting the kinesin MKLP1, a key subunit of the central spindlin complex. Hisayoshi Makyio, Minako Ohgi, Tomomi Takei, Senye Takahashi, Hiroyuki Takatsu, Yohei Katoh, Ayako Hanai, Tomoko Ueda, Yasunori Kanaho, Yong Xie, Hye-Won Shin, Hironari Kamikubo, Mikio Kataoka, Masato Kawasaki, Ryuichi Kato, Soichi Wakatsuki and Kazuhisa Nakayama The EMBO Journal (2012), 31, 2590 - 2603; 10.1038/emboj.2012.89 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 20 April 2012 ARF6 GTPase protects the post-mitotic midbody from 14-3-3-mediated disintegration The endosomal GTPase ARF6 competes with the negative regulator 14-3-3 for binding to the centralspindlin component MKLP1, representing a new regulator of this complex required for abscission during cytokinesis. Nimesh Joseph, Andrea Hutterer, Ina Poser and Masanori Mishima The EMBO Journal (2012), 31, 2604 - 2614; 10.1038/emboj.2012.139 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 11 May 2012 Caspase-2 is an initiator caspase responsible for pore-forming toxin-mediated apoptosis Caspase-2 is one of the first discovered, most conserved mammalian caspases with a so far enigmatic biological function. The results presented here establish caspase-2 as critical effector of PFT-mediated apoptosis in epithelial cells. Gergely Imre, Jan Heering, Armelle-Natsuo Takeda, Matthias Husmann, Bernd Thiede, Dagmar Meyer zu Heringdorf, Douglas R Green, F Gisou van der Goot, Bhanu Sinha, Volker Dötsch and Krishnaraj Rajalingam The EMBO Journal (2012), 31, 2615 - 2628; 10.1038/emboj.2012.93 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 24 April 2012 Distinct requirement for an intact dimer interface in wild-type, V600E and kinase-dead B-Raf signalling An intact dimer interface (DIF) is required for both signalling and dimerisation in wild-type Raf, but surprisingly for neither in certain oncogenic Raf versions. Paradoxical Raf activation reveals an additional layer of complexity, with the DIF now dispensable for dimerisation but required for signalling. Michael Röring, Ricarda Herr, Gina J Fiala, Katharina Heilmann, Sandra Braun, Anja E Eisenhardt, Sebastian Halbach, David Capper, Andreas von Deimling, Wolfgang W Schamel, Darren N Saunders and Tilman Brummer The EMBO Journal (2012), 31, 2629 - 2647; 10.1038/emboj.2012.100 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 17 April 2012 The inner membrane histidine kinase EnvZ senses osmolality via helix-coil transitions in the cytoplasm The bacterial two-component system EnvZ/OmpR governs the osmotic stress response. Increasing osmolality enhances intrahelical H-bonding in the transmembrane kinase EnvZ, leading to helix stabilization, autophosphorylation and activation of downstream signalling. Loo Chien Wang, Leslie K Morgan, Pahan Godakumbura, Linda J Kenney and Ganesh S Anand The EMBO Journal (2012), 31, 2648 - 2659; 10.1038/emboj.2012.99 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 27 April 2012 Corrigendum Top IKK and NF-κB-mediated regulation of Claspin impacts on ATR checkpoint function Niall Steven Kenneth, Sharon Mudie and Sonia Rocha The EMBO Journal (2012), 31, 2660 - 2661; 10.1038/emboj.2012.135 Abstract | Full text | PDF Published online: 30 May 2012 Advertisement Nature Genetics and the Wellcome Trust present: The Genomics of Common Diseases 2012 September 19-22, 2012 - Potomac, MD, USA For more information and to register, visit: www.nature.com/natureconferences/gcd2012 Abstract submission deadline- June 28, 2012   Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to The EMBO Journal. In order to do so, please purchase a subscription. 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