Advertisement | | Nikon's new high-speed N-SIM S super-resolution microscope system achieves acquisition speeds up to 15 frames per second, enabling the acquisition of fast biological processes at twice the spatial resolution of conventional light microscopes. Learn more | | | | | | TABLE OF CONTENTS
| August 2018 Volume 15, Issue 8 | | | | | Editorial This Month Correspondence Research Highlights Technology Feature News & Views Brief Communications Articles | | Advertisement | | Hamamatsu's W-View Gemini-2C: Multi-wavelength, multi-camera imaging that's built for super-resolution and ready for anything. Now available with our new Extended Focus Device increasing depth of focus up to 5x by using a simple optical filter. | | | | | | Editorial | | | | | The wisdom of crowds, for a fee p555 doi:10.1038/s41592-018-0101-4 | | This Month | | | | | Ilaria Testa p557 Vivien Marx doi:10.1038/s41592-018-0078-z | | | | Optimal experimental design pp559 - 560 Byran Smucker, Martin Krzywinski & Naomi Altman doi:10.1038/s41592-018-0083-2 | | Advertisement | | Advanced Imaging with Proven Optics Olympus is dedicated to your work, vision, and science. Through innovation and service, we seek to aide in your discoveries, advance your research, and inspire you to explore new possibilities. Our wide range of microscopes are built with the optical excellence and proven application expertise you can depend on. Your Science Matters™. > Learn more about Olympus microscopes | | |
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Correspondence | |
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Water content, not stiffness, dominates Brillouin spectroscopy measurements in hydrated materials pp561 - 562 Pei-Jung Wu, Irina V. Kabakova, Jeffrey W. Ruberti, Joseph M. Sherwood, Iain E. Dunlop et al. doi:10.1038/s41592-018-0076-1 |
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Reply to ‘Water content, not stiffness, dominates Brillouin spectroscopy measurements in hydrated materials’ pp562 - 563 Giuliano Scarcelli & Seok Hyun Yun doi:10.1038/s41592-018-0075-2 |
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BraCeR: B-cell-receptor reconstruction and clonality inference from single-cell RNA-seq pp563 - 565 Ida Lindeman, Guy Emerton, Lira Mamanova, Omri Snir, Krzysztof Polanski et al. doi:10.1038/s41592-018-0082-3 |
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Research Highlights | |
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Technology Feature | |
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Calling cell biologists to try cryo-ET pp575 - 578 Vivien Marx doi:10.1038/s41592-018-0079-y |
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News & Views | |
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The crowd storms the ivory tower pp579 - 580 Martin L. Jones & Helen Spiers doi:10.1038/s41592-018-0077-0 |
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Finally: development of humanized lymph nodes pp580 - 582 Alexandre P. A. Theocharides & Markus G. Manz doi:10.1038/s41592-018-0080-5 |
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Brief Communications | |
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Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections pp583 - 586 Michael J. Mlodzianoski, Paul J. Cheng-Hathaway, Shane M. Bemiller, Tyler J. McCray, Sheng Liu et al. doi:10.1038/s41592-018-0053-8 Active PSF shaping and adaptive optics are combined to enable 3D localization microscopy throughout thick tissues. The method was used to study the nanoscale architecture of amyloid fibrils in a mouse model of Alzheimer’s disease. |
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Quanti.us: a tool for rapid, flexible, crowd-based annotation of images pp587 - 590 Alex J. Hughes, Joseph D. Mornin, Sujoy K. Biswas, Lauren E. Beck, David P. Bauer et al. doi:10.1038/s41592-018-0069-0 Annotated image data are required for image analysis, to test analytical methods, and to train learning algorithms. This paper describes and characterizes a tool that allows researchers to crowdsource image-annotation tasks. |
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Strelka2: fast and accurate calling of germline and somatic variants pp591 - 594 Sangtae Kim, Konrad Scheffler, Aaron L. Halpern, Mitchell A. Bekritsky, Eunho Noh et al. doi:10.1038/s41592-018-0051-x Strelka2 incorporates improvements for fast and accurate calling of somatic and germline single-nucleotide variants and indels. |
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A synthetic-diploid benchmark for accurate variant-calling evaluation pp595 - 597 Heng Li, Jonathan M. Bloom, Yossi Farjoun, Mark Fleharty, Laura Gauthier et al. doi:10.1038/s41592-018-0054-7 The synthetic-diploid (Syndip) benchmark dataset, constructed from two fully homozygous long-read assemblies, provides more accurate assessments of error rates in small-variant-calling algorithms than existing benchmarks. |
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Genome-wide C-SWAT library for high-throughput yeast genome tagging pp598 - 600 Matthias Meurer, Yuanqiang Duan, Ehud Sass, Ilia Kats, Konrad Herbst et al. doi:10.1038/s41592-018-0045-8 A C-SWAT acceptor library allows the user to easily insert any tag of choice after yeast ORFs by swapping it for the acceptor module. |
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Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy pp601 - 604 Francesca Pennacchietti, Ekaterina O. Serebrovskaya, Aline R. Faro, Irina I. Shemyakina, Nina G. Bozhanova et al. doi:10.1038/s41592-018-0052-9 Bright reversibly switching red fluorescent proteins (rsFusionReds) with fast switching kinetics and low fatigue enable RESOLFT and MoNaLISA nanoscopy of live cells with green-orange illumination, which further reduces the risk of phototoxicity. |
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Articles | |
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High-precision automated reconstruction of neurons with flood-filling networks pp605 - 610 Michał Januszewski, Jörgen Kornfeld, Peter H. Li, Art Pope, Tim Blakely et al. doi:10.1038/s41592-018-0049-4 Flood-filling networks are a deep-learning-based pipeline for reconstruction of neurons from electron microscopy datasets. The approach results in exceptionally low error rates, thereby reducing the need for extensive human proofreading. |
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An enhanced CRISPR repressor for targeted mammalian gene regulation pp611 - 616 Nan Cher Yeo, Alejandro Chavez, Alissa Lance-Byrne, Yingleong Chan, David Menn et al. doi:10.1038/s41592-018-0048-5 The fusion of dead Cas9 with KRAB and the transcriptional repressor domain of the chromatin modifier MeCP2 leads to an efficient transcriptional silencer that can be applied to genome-scale screens and genetic circuits. |
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Genome-wide SWAp-Tag yeast libraries for proteome exploration pp617 - 622 Uri Weill, Ido Yofe, Ehud Sass, Bram Stynen, Dan Davidi et al. doi:10.1038/s41592-018-0044-9 A genome-wide collection of N-terminally tagged yeast libraries allows easy swapping of tags and exploration of the yeast proteome. |
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A human immune system mouse model with robust lymph node development pp623 - 630 Yan Li, Guillemette Masse-Ranson, Zacarias Garcia, Timothée Bruel, Ayrin Kök et al. doi:10.1038/s41592-018-0071-6 Humanized mouse models are useful for studies of human hematopoiesis and immunity. Li et al. report an improved model that harbors lymph nodes and therefore permits investigation of local human adaptive immune processes in secondary lymphoid tissue. |
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Genetically engineered cerebral organoids model brain tumor formation pp631 - 639 Shan Bian, Marko Repic, Zhenming Guo, Anoop Kavirayani, Thomas Burkard et al. doi:10.1038/s41592-018-0070-7 Cerebral organoids are developed into in vitro models of human brain cancer by CRISPR–Cas9- and/or transposon-mediated introduction of oncogenic mutations. |
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