Nature Methods Application Notes e-UPDATE: 12 September 2017

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12 September 2017  Application Notes bring you the latest information about innovative tools and technologies and their applications in the lab. We hope that you will find this service useful and informative and encourage you to sign up for future Updates to ensure that you never miss one!

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FEATURED APPLICATION NOTE Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening www.thermofisher.com/hcs > Synapse formation during nervous system development and degeneration in the pathogenesis of human neurological diseases are highly regulated processes. Subtle changes in the environment of the complex neuronal network may cause either breakdown or creation of synaptic connections. Drug discovery screening for neurological diseases and compound neurotoxicity evaluation would benefit from robust, automated, quantitative in vitro assays that monitor neuronal function. PDF

		A study on the efficacy of Prime&Bond active, the new active universal adhesive from Dentsply Sirona www.dentsplysirona.com/en-gb/products/restorative/prime-&-bond-active.html > Prime&Bond active™ is a one-component universal dental adhesive which brings new advantages to the universal adhesive arena. For effective bonding, conventional Universal Dental adhesives require ideal moisture conditions to form a homogeneous adhesive layer, and in turn a strong bond between tooth and restoration. However, when too much or too little moisture is present this may not happen and bonding can fail. Prime&Bond active contains "Active-Guard" technology, which gives this adhesive smart properties that compensate for variations in moisture levels and ensure complete adhesive coverage of the tooth. PDF

		Quantitative live-cell analysis for optimization of culture conditions and evaluation of cell health in human induced pluripotent stem cell-derived neurons www.essenbioscience.com/en/ > In this application note, we describe methods and present validation data highlighting optimal culture conditions for evaluation of cell viability and neurite outgrowth in hiPSCderived neurons from Cellular Dynamic International (CDI, iCell Neurons). We also monitor neurite outgrowth and cellular viability in iCell Gluta Neurons from CDI using a quantitative, live-cell imaging and analysis approach with the IncuCyte® S3 over days/weeks in 96-well microplate culture. To exemplify a real-time imaging and analysis approach using hiPSCderived neurons, we assess neuronal excitotoxicity using the IncuCyte® S3 Phase/Fluorescent NeuroTrack applications multiplexed with Annexin V reagents. These assays outline optimal culture conditions for an example iPSC-derived neuronal system and demonstrate the ability of the IncuCyte approach for real-time, long-term quantitative analysis ofiPSC-derived neuronal cell health. PDF

		A humanized phenotypic screening platform for chronic pain www.censobio.com > www.Cellectricon.com > Censo Biotechnologies and Cellectricon have joined forces to develop a first-in-kind humanized drug discovery platform to target chronic pain. The platform is based on human induced pluripotent stem cell–derived neurons in combination with Cellectricon's Cellaxess® Elektra screening system. The combination of highly functional screening with a human cell type of relevance for chronic pain has the potential to enable the generation of better drug candidates for this condition and other diseases. PDF

		Gibson Assembly® Primer-Bridge End Joining (PBnJ™) Cloning www.syntheticgenomics.com > Gibson Assembly® cloning has proven to be useful as a molecular biology technique for the seamless assembly of synthetic and natural genes and large-scale genetic pathways. Notably, Gibson Assembly cloning has enabled the synthesis of the first bacterial genome1, the first synthetic cell2, and the first minimal cell3. Additionally, the Gibson Assembly method has been utilized for genetic recoding (genome-wide codon removal)4, the engineering of Cre recombinase for improved site-specificity5, and has been incorporated into a one-step method for cloning gRNA for the CRISPR-Cas9 system6. PDF

		Turning neuroscience data into insight in the 21st century www.metacell.us > The modern research and industrial approach to neuroscience involves an increasingly vast assortment of data collection methods and data handling approaches. While data collection and accumulation are expanding, approaches to data management and insight gathering are still lacking. PDF

		Match & Scratch Barcodes: Tools for the Demultiplexing and Extraction of Target Sequences from PacBio Amplicon Data www.jax.org > One of the major challenges for the downstream analysis of amplicon data is to first demultiplex Fastq files based on the ligation of different oligonucleotides combinations. Match & Scratch Barcodes are a set of bioinformatics tools that support the analysis of PacBio sequenced long read amplicon data by detecting forward and reverse end adapter sequences, generic adapters attachedto the region specific oligos, multiple number of region specific oligos of variable length for the extraction of sequences of interest. PDF

 

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