Nature Biotechnology Contents: Volume 35 pp 801 - 888

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TABLE OF CONTENTS September 2017 Volume 35, Issue 9 Editorial News Opinion and Comment Features News and Views Research Careers and Recruitment Advertisement   Technical Paper: Streamline Production of Complex Proteins Producing high quality, functional proteins in a rapid and cost-effective manner can be challenging when dealing with complex or difficult-to-express proteins, particularly if cGMP-compliance is needed. Find out how implementing the right expression platform can transform your efforts.  View Now Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement Free Naturejobs Career Expo London - registration is now open  The 11th annual Naturejobs Career Expo will be held in London on October 4, 2017. Join us for your chance to network with leading global employers, receive one-to-one CV checking, attend free talks and workshops, learn how to enhance your employability and lots more.  Register FREE today!    Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement KAUST Discovery: Research into sustainable agriculture   Plant Scientist Mark Tester’s research into growing crops on poor-quality soils or on otherwise barren land is discovering some ground-breaking findings.   Find out more on how Tester’s research could one day help feed the world.   Read more here >>   Advertisement An open access, online-only, multidisciplinary research journal dedicated to publishing the most important scientific advances in the life sciences, physical sciences, and engineering fields that are facilitated by spaceflight and analogue platforms.   Explore the benefits of submitting your next research article.   Editorial Top Buying time for transplants   p801 doi:10.1038/nbt.3971 Funders need to pay more attention to research aimed at increasing the shelf life of human organs. Doing so could pay dividends for both transplantation and basic research. News Top Blood feud erupts over Roche's bispecific antibody for hemophilia   pp803 - 804 Elie Dolgin doi:10.1038/nbt0917-803 Adenosine checkpoint agent blazes a trail, joins immunotherapy roster   pp805 - 807 Ken Garber doi:10.1038/nbt0917-805 Go-ahead for first anti-IL-23 mAb to treat psoriasis   p806 doi:10.1038/nbt0917-806 Vertex CF data wow Wall Street   p807 doi:10.1038/nbt0917-807 Nestle moves into gut microbiome tests   p808 doi:10.1038/nbt0917-808a CRISPR patent estate splinters   pp808 - 809 Cormac Sheridan doi:10.1038/nbt0917-808b Massachusetts injects more cash into jobs   p809 doi:10.1038/nbt0917-809 What's app? Helix wants you to quiz your genome—some of it for fun   p810 Laura DeFrancesco doi:10.1038/nbt0917-810 Podcast Podcast: First rounders: Greg Winter   p811 doi:10.1038/nbt.3950 Around the world in a month   p811 doi:10.1038/nbt0917-811 Data Page 2Q17—Biotech stages a recovery   p812 Laura DeFrancesco and Brady Huggett doi:10.1038/nbt.3949 Opinion and Comment Top Correspondence Challenges in the gene therapy commercial ecosystem   pp813 - 815 Rahul Kapoor, Thomas Klueter and James M Wilson doi:10.1038/nbt.3931 Neoantigen prediction and the need for validation   pp815 - 817 Antonella Vitiello and Maurizio Zanetti doi:10.1038/nbt.3932 Greener revolutions for all require transparency and diversity, not secrecy   pp817 - 818 Morten Hedegaard Larsen doi:10.1038/nbt.3951 Reply: Greener revolutions for all require transparency and diversity, not secrecy   pp818 - 819 Richard B Flavell doi:10.1038/nbt.3952 Fast Tracking Biotherapeutic Development Using advanced cell engineering for large-scale transient expression, streamlined cell line generation and genome modification can significantly accelerate the development and bioproduction of your biotherapeutic candidates.  Learn more. Features Top Patents After Myriad, what makes a gene patent claim 'markedly different' from nature?   pp820 - 825 Mateo Aboy, Johnathon Liddicoat, Kathleen Liddell, Matthew Jordan and Cristina Crespo doi:10.1038/nbt.3953 Examining the types of claim amendments that have transformed isolated gene claims from patent-ineligible into eligible subject matter provides clarity into the threshold of eligibility for gene-related patents. Recent patents related to clinical applications of sequencing   p826 doi:10.1038/nbt.3970 nature.com webcasts Nature Biotechnology webcast on CAR-T cells: Latest advances and outstanding challenges Duration: 60 minutes with an opportunity to ask questions during the live broadcast! Date: Wednesday, September 20th, 2017 Time: 9 AM PDT| 12 PM EDT | 5 PM BST | 6 PM CEST  Register for FREE Sponsored by:  Lonza   News and Views Top Tagging activated neurons with light   pp827 - 828 Dheeraj S Roy, Teruhiro Okuyama and Susumu Tonegawa doi:10.1038/nbt.3954 Two new protein tools translate neuronal activity into gene expression during a light-defined time window. See also: Research by Lee et al. | Research by Wang et al. Interpreting the T-cell receptor repertoire   pp829 - 830 Robert A Holt doi:10.1038/nbt.3957 T cell receptor sequence motifs can predict T cell antigen specificity. Research Highlights   p830 doi:10.1038/nbt.3961 Mouse phenotyping sheds light on rare disease   p831 Sarah Perry doi:10.1038/nbt.3959 Precision editing in the human embryo   p832 Kathy Aschheim doi:10.1038/nbt.3965 Biotechnology JOBS of the week Head (m / f) of Skill Center Advanced Data Analysis and Predictive Process Sciences Sanofi Aventis GmbH One faculty, five postdoc and graduate student positions in Biofuels and Biorefinery Nanjing Agricultural University (NAU) Biological Safety Officer at The University of Hong Kong The University of Hong Kong PhD student / candidate, project on nanobodies / invadosomes / protein interference / super resolution microscopy. Ghent University Open phd position in the Integrative cell signalling group Luxembourg University More Science jobs from Research Top Review Shotgun metagenomics, from sampling to analysis   pp833 - 844 Christopher Quince, Alan W Walker, Jared T Simpson, Nicholas J Loman and Nicola Segata doi:10.1038/nbt.3935 The promises and potential pitfalls of shotgun metagenomics, from experimental design to computational analyses, are reviewed. Article Control of phosphorothioate stereochemistry substantially increases the efficacy of antisense oligonucleotides   pp845 - 851 Naoki Iwamoto, David C D Butler, Nenad Svrzikapa, Susovan Mohapatra, Ivan Zlatev et al. doi:10.1038/nbt.3948 A scalable chemical method to control phosphorothioate chirality demonstrates its impact on the efficacy of antisense oligonucleotides. Letters Haplotype phasing of whole human genomes using bead-based barcode partitioning in a single tube   pp852 - 857 Fan Zhang, Lena Christiansen, Jerushah Thomas, Dmitry Pokholok, Ros Jackson et al. doi:10.1038/nbt.3897 Haplotype information for whole genomes is rapidly generated with a single-tube method. A calcium- and light-gated switch to induce gene expression in activated neurons   pp858 - 863 Dongmin Lee, Jung Ho Hyun, Kanghoon Jung, Patrick Hannan and Hyung-Bae Kwon doi:10.1038/nbt.3902 Gene expression is controlled in activated neurons in the mouse brain using a two-component optogenetic system. See also: News and Views by Roy et al. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons   pp864 - 871 Wenjing Wang, Craig P Wildes, Tanyaporn Pattarabanjird, Mateo I Sanchez, Gordon F Glober et al. doi:10.1038/nbt.3909 Activity remodels neurons, altering their molecular, structural, and electrical characteristics. To enable the selective characterization and manipulation of these neurons, we present FLARE, an engineered transcription factor that drives expression of fluorescent proteins, opsins, and other genetically encoded tools only in the subset of neurons that experienced activity during a user-defined time window. FLARE senses the coincidence of elevated cytosolic calcium and externally applied blue light, which together produce translocation of a membrane-anchored transcription factor to the nucleus to drive expression of any transgene. In cultured rat neurons, FLARE gives a light-to-dark signal ratio of 120 and a high- to low-calcium signal ratio of 10 after 10 min of stimulation. Opsin expression permitted functional manipulation of FLARE-marked neurons. In adult mice, FLARE also gave light- and motor-activity-dependent transcription in the cortex. Due to its modular design, minute-scale temporal resolution, and minimal dark-state leak, FLARE should be useful for the study of activity-dependent processes in neurons and other cells that signal with calcium. See also: News and Views by Roy et al. Resources An integrated expression atlas of miRNAs and their promoters in human and mouse   pp872 - 878 Derek de Rie, Imad Abugessaisa, Tanvir Alam, Erik Arner, Peter Arner et al. doi:10.1038/nbt.3947 An atlas of microRNA expression patterns and regulators is produced by deep sequencing of short RNAs in human and mouse cells. An atlas of B-cell clonal distribution in the human body   pp879 - 884 Wenzhao Meng, Bochao Zhang, Gregory W Schwartz, Aaron M Rosenfeld, Daqiu Ren et al. doi:10.1038/nbt.3942 B-cell VH region repertoire sequencing of eight anatomical sites in six human donors reveals distinct networks of clone distribution. Careers and Recruitment Top What faculty hiring committees want   pp885 - 887 Charles B Wright and Nathan L Vanderford doi:10.1038/nbt.3962 PhD trainees aspiring to become faculty need to know the credentials search committees value most in an applicant. People People   p888 doi:10.1038/nbt.3969 Advertisement npj Molecular Phenomics is an online-only, open access journal that provides a forum for cutting-edge scientific advances in the emerging field of phenomics, the study of the physical and chemical characteristics of an individual in quantitative terms. Part of the Nature Partner Journals series, npj Molecular Phenomics is published in partnership with Fudan University. The journal is now open for submissions.   Find out more >>>   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. 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