Laboratory Investigation - Table of Contents alert Volume 97 Issue 5

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TABLE OF CONTENTS Volume 97, Issue 5 (May 2017) In this issue Inside the USCAP Journals Pathobiology in Focus Mini Review Research Articles Technical Report Also new AOP Like us on Facebook Twitter Sign up for e-alerts Recommend to your library Web feed Subscribe Inside the USCAP Journals Top Inside the USCAP Journals 2017 97: 492-493; 10.1038/labinvest.2017.38 Full Text Pathobiology in Focus Top Drugs with anti-oxidant properties can interfere with cell viability measurements by assays that rely on the reducing property of viable cells The authors demonstrate that drugs with anti-oxidant properties can interfere significantly with cell viability measurements when assays that rely on the reducing property of viable cells are used to assess their cytotoxic potential, when performed according to the current manufacturer recommended protocol. This interference can be prevented with the addition of a simple step in the protocol. Niraj Shenoy, Mary Stenson, Joshua Lawson, Jithma Abeykoon, Mrinal Patnaik, Xiaosheng Wu and Thomas Witzig 2017 97: 494-497; advance online publication, February 27, 2017; 10.1038/labinvest.2017.18 Abstract | Full Text Mini Review Top Immune microenvironment of gliomas Malignant gliomas are aggressive brain tumors that are heavily infiltrated with myeloid cells deficient in antitumor activity but supportive of tumor invasion. This review summarizes data on immune cell heterogeneity and cell-cell interactions in the tumor microenvironment, as well as tumor-derived signals and mechanisms driving myeloid cell accumulation and reprogramming. Anna Gieryng, Dominika Pszczolkowska, Kacper A Walentynowicz, Wenson D Rajan and Bozena Kaminska 2017 97: 498-518; advance online publication, March 13, 2017; 10.1038/labinvest.2017.19 Abstract | Full Text Research Articles Top HEPATIC AND GASTROINTESTINAL SYSTEMS Tauroursodeoxycholic acid protects bile acid homeostasis under inflammatory conditions and dampens Crohn’s disease-like ileitis The authors show that the bile acid tauroursodeoxycholic acid alleviates inflammation-induced downregulation of ileal bile acid transporters and nuclear receptors, which are also downregulated in Crohn's disease patients, and suppresses murine Crohn's disease-like ileitis. Therefore, tauroursodeoxycholic acid could be a promising therapeutic agent for inflammatory bowel disease. Lien Van den Bossche, Daniel Borsboom, Sarah Devriese, Sophie Van Welden, Tom Holvoet, Lindsey Devisscher, Pieter Hindryckx, Martine De Vos and Debby Laukens 2017 97: 519-529; advance online publication, February 6, 2017; 10.1038/labinvest.2017.6 Abstract | Full Text MiRNA expression profiles reveal the involvement of miR-26a, miR-548l and miR-34a in hepatocellular carcinoma progression through regulation of ST3GAL5 This study investigates the miRNA expressional profiles in highly invasive human hepatocellular carcinoma (HCC) cell line MHCC97-H and MHCC97-L with lower metastasis potential. The results show that13 differentially expressed miRNAs are identified, ST3GAL5 is the direct target of miR-26a, miR-548l and miR-34a. Both ST3GAL5 and these three miRNAs are found to be involved in progression of HCC cells. Hongjiao Cai, Huimin Zhou, Yuan Miao, Nana Li, Lifen Zhao and Li Jia 2017 97: 530-542; advance online publication, February 20, 2017; 10.1038/labinvest.2017.12 Abstract | Full Text ANGIOGENESIS, CARDIOVASCULAR AND PULMONARY SYSTEMS Maresin1 stimulates alveolar fluid clearance through the alveolar epithelial sodium channel Na,K-ATPase via the ALX/PI3K/Nedd4-2 pathway Edema fluid resorption is critical for gas exchange. In this study, the authors demonstrate that MaR1(MaR1) promotes the resolution of inflammation alleviated pulmonary edema, enhances alveolar fluid clearance, and attenuates lung injury partially through stimulation of the epithelial sodium channel and Na, K-ATPase via activation of the ALX/PI3K/Nedd4-2 pathway in acute lung injury. These findings reveal a novel mechanism for pulmonary edema fluid reabsorption and MaR1 may provide a new therapy for the resolution of acute lung injury and acute respiratory distress syndrome. Jun-Li Zhang, Xiao-Jun Zhuo, Jing Lin, Ling-Chun Luo, Wei-Yang Ying, Xiang Xie, Hua-Wei Zhang, Jing-Xiang Yang, Dan Li, Fang Gao Smith and Sheng-Wei Jin 2017 97: 543-554; advance online publication, February 20, 2017; 10.1038/labinvest.2016.150 Abstract | Full Text GENITOURINARY AND REPRODUCTIVE SYSTEMS Iron suppresses erythropoietin expression via oxidative stress-dependent hypoxia-inducible factor-2 alpha inactivation Iron is widely used for treatment of anemia in chronic kidney disease (CKD). However, excess iron causes organ damage through oxidative stress production. In this study, iron supplementation suppresses erythropoietin through oxidative stress-mediated hypoxia-inducible factor-2a inactivation, suggesting a latent risk of parenteral iron supplementation in endogenous erythropoietin production in CKD. Keisuke Oshima, Yasumasa Ikeda, Yuya Horinouchi, Hiroaki Watanabe, Hirofumi Hamano, Yoshitaka Kihira, Seiji Kishi, Yuki Izawa-Ishizawa, Licht Miyamoto, Tasuku Hirayama, Hideko Nagasawa, Keisuke Ishizawa, Koichiro Tsuchiya and Toshiaki Tamaki 2017 97: 555-566; advance online publication, March 6, 2017; 10.1038/labinvest.2017.11 Abstract | Full Text Epithelial–mesenchymal transition promotes SOX2 and NANOG expression in bladder cancer Epithelial-mesenchymal transition (EMT) has been suggested as a potential cancer stemness trigger. The authors found that TGF-β induced-EMT increased SOX2 and Nanog protein levels in bladder cancer cells. Immunohistochemistry showed a mutually exclusive expression of E-cadherin and SOX2/Nanog. These findings suggest that EMT promotes stemness of bladder cancer, contributing to tumor aggressiveness. Toshiro Migita, Ayano Ueda, Tomokazu Ohishi, Megumi Hatano, Hiroyuki Seimiya, Shin-ichiro Horiguchi, Fumitaka Koga and Futoshi Shibasaki 2017 97: 567-576; advance online publication, February 27, 2017; 10.1038/labinvest.2017.17 Abstract | Full Text ENDOCRINE, VISUAL AND AUDITORY SYSTEMS Suppression of islet homeostasis protein thwarts diabetes mellitus progression Islet homeostasis protein (IHoP) co-localizes within pancreatic a-cells and is over-expressed in the islets of post-onset non-obese diabetic (NOD) mice and Type-1 diabetic patients. Suppression of IHoP during pre-diabetes altered the immune response producing normoglycemia in NOD mice for 35 weeks. IHoP levels increase in diabetic serum, indicating possible application as a biomarker. Seh-Hoon Oh, Marda L Jorgensen, Clive H Wasserfall, Altin Gjymishka and Bryon E Petersen 2017 97: 577-590; advance online publication, February 20, 2017; 10.1038/labinvest.2017.15 Abstract | Full Text DEVELOPMENT, MATRIX BIOLOGY AND AGING Increased expression of latent TGF-β-binding protein 4 affects the fibrotic process in scleroderma by TGF-β/SMAD signaling Scleroderma is a fibrosis-related disorder in which TGF-β plays a central role and latent TGF-β binding protein 4 (LTBP-4) affects the secretion of latent TGF-β to the extracellular matrix. Increased expression of LTBP-4 is found in serum and skin tissues of scleroderma patients, and its knockdown downregulates the secretion of TGF-β and activation of TGF-β/SMAD signaling. LTBP-4 plays roles in scleroderma by regulating TGF-β secretion. Jiaying Lu, Qingmei Liu, Lei Wang, Wenzhen Tu, Haiyan Chu, Weifeng Ding, Shuai Jiang, Yanyun Ma, Xiangguang Shi, Weilin Pu, Xiaodong Zhou, Li Jin, Jiucun Wang and Wenyu Wu 2017 97: 591-601; advance online publication, March 6, 2017; 10.1038/labinvest.2017.20 Abstract | Full Text BLOOD, LYMPHATICS, IMMUNE SYSTEM AND STEM CELLS Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation Dengue virus (DENV) enhances degranulation of mast cells and production of chemokines (MCP-1, RANTES, and IP-10) in a mouse model. Anti-modified DENV NS1 (DJ NS1) antibodies reduce mast cell degranulation and production of DENV-induced chemokines. Therefore, antibodies against NS1 protein provide therapeutic benefits, which involve modulating DENV-induced mast cell activation. Ya-Ting Chu, Shu-Wen Wan, Yu-Chang Chang, Chien-Kuo Lee, Betty A Wu-Hsieh, Robert Anderson and Yee-Shin Lin 2017 97: 602-614; advance online publication, February 27, 2017; 10.1038/labinvest.2017.10 Abstract | Full Text Technical Report Top MODELS AND TECHNIQUES Blue intensity matters for cell cycle profiling in fluorescence DAPI-stained images Cell cycle deregulation is associated to several human pathologies, including cancer. The authors developed a method of image analysis based on DAPI-staining which allows the identification of the cell cycle momentum of individual and population of cells. Overall, the imaging framework exhibited a sensitivity of 94.0% and a specificity of 93.9% in discretizing the cell cycle phases of unperturbed, digitized cells. Anabela Ferro, Tânia Mestre, Patrícia Carneiro, Ivan Sahumbaiev, Raquel Seruca and João M Sanches 2017 97: 615-625; advance online publication, March 6, 2017; 10.1038/labinvest.2017.13 Abstract | Full Text Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department. For print subscription enquiries, please contact our subscription department. For other enquiries, please contact our customer feedback department. 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