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Laboratory Investigation - Table of Contents alert Volume 97 Issue 5

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Laboratory Investigation


Volume 97, Issue 5 (May 2017)

In this issue
Inside the USCAP Journals
Pathobiology in Focus
Mini Review
Research Articles
Technical Report

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Inside the USCAP Journals


Inside the USCAP Journals

2017 97: 492-493; 10.1038/labinvest.2017.38

Full Text

Pathobiology in Focus


Drugs with anti-oxidant properties can interfere with cell viability measurements by assays that rely on the reducing property of viable cells

The authors demonstrate that drugs with anti-oxidant properties can interfere significantly with cell viability measurements when assays that rely on the reducing property of viable cells are used to assess their cytotoxic potential, when performed according to the current manufacturer recommended protocol. This interference can be prevented with the addition of a simple step in the protocol.

Niraj Shenoy, Mary Stenson, Joshua Lawson, Jithma Abeykoon, Mrinal Patnaik, Xiaosheng Wu and Thomas Witzig

2017 97: 494-497; advance online publication, February 27, 2017; 10.1038/labinvest.2017.18

Abstract | Full Text

Mini Review


Immune microenvironment of gliomas

Malignant gliomas are aggressive brain tumors that are heavily infiltrated with myeloid cells deficient in antitumor activity but supportive of tumor invasion. This review summarizes data on immune cell heterogeneity and cell-cell interactions in the tumor microenvironment, as well as tumor-derived signals and mechanisms driving myeloid cell accumulation and reprogramming.

Anna Gieryng, Dominika Pszczolkowska, Kacper A Walentynowicz, Wenson D Rajan and Bozena Kaminska

2017 97: 498-518; advance online publication, March 13, 2017; 10.1038/labinvest.2017.19

Abstract | Full Text

Research Articles



Tauroursodeoxycholic acid protects bile acid homeostasis under inflammatory conditions and dampens Crohn’s disease-like ileitis

The authors show that the bile acid tauroursodeoxycholic acid alleviates inflammation-induced downregulation of ileal bile acid transporters and nuclear receptors, which are also downregulated in Crohn's disease patients, and suppresses murine Crohn's disease-like ileitis. Therefore, tauroursodeoxycholic acid could be a promising therapeutic agent for inflammatory bowel disease.

Lien Van den Bossche, Daniel Borsboom, Sarah Devriese, Sophie Van Welden, Tom Holvoet, Lindsey Devisscher, Pieter Hindryckx, Martine De Vos and Debby Laukens

2017 97: 519-529; advance online publication, February 6, 2017; 10.1038/labinvest.2017.6

Abstract | Full Text

MiRNA expression profiles reveal the involvement of miR-26a, miR-548l and miR-34a in hepatocellular carcinoma progression through regulation of ST3GAL5

This study investigates the miRNA expressional profiles in highly invasive human hepatocellular carcinoma (HCC) cell line MHCC97-H and MHCC97-L with lower metastasis potential. The results show that13 differentially expressed miRNAs are identified, ST3GAL5 is the direct target of miR-26a, miR-548l and miR-34a. Both ST3GAL5 and these three miRNAs are found to be involved in progression of HCC cells.

Hongjiao Cai, Huimin Zhou, Yuan Miao, Nana Li, Lifen Zhao and Li Jia

2017 97: 530-542; advance online publication, February 20, 2017; 10.1038/labinvest.2017.12

Abstract | Full Text


Maresin1 stimulates alveolar fluid clearance through the alveolar epithelial sodium channel Na,K-ATPase via the ALX/PI3K/Nedd4-2 pathway

Edema fluid resorption is critical for gas exchange. In this study, the authors demonstrate that MaR1(MaR1) promotes the resolution of inflammation alleviated pulmonary edema, enhances alveolar fluid clearance, and attenuates lung injury partially through stimulation of the epithelial sodium channel and Na, K-ATPase via activation of the ALX/PI3K/Nedd4-2 pathway in acute lung injury. These findings reveal a novel mechanism for pulmonary edema fluid reabsorption and MaR1 may provide a new therapy for the resolution of acute lung injury and acute respiratory distress syndrome.

Jun-Li Zhang, Xiao-Jun Zhuo, Jing Lin, Ling-Chun Luo, Wei-Yang Ying, Xiang Xie, Hua-Wei Zhang, Jing-Xiang Yang, Dan Li, Fang Gao Smith and Sheng-Wei Jin

2017 97: 543-554; advance online publication, February 20, 2017; 10.1038/labinvest.2016.150

Abstract | Full Text


Iron suppresses erythropoietin expression via oxidative stress-dependent hypoxia-inducible factor-2 alpha inactivation

Iron is widely used for treatment of anemia in chronic kidney disease (CKD). However, excess iron causes organ damage through oxidative stress production. In this study, iron supplementation suppresses erythropoietin through oxidative stress-mediated hypoxia-inducible factor-2a inactivation, suggesting a latent risk of parenteral iron supplementation in endogenous erythropoietin production in CKD.

Keisuke Oshima, Yasumasa Ikeda, Yuya Horinouchi, Hiroaki Watanabe, Hirofumi Hamano, Yoshitaka Kihira, Seiji Kishi, Yuki Izawa-Ishizawa, Licht Miyamoto, Tasuku Hirayama, Hideko Nagasawa, Keisuke Ishizawa, Koichiro Tsuchiya and Toshiaki Tamaki

2017 97: 555-566; advance online publication, March 6, 2017; 10.1038/labinvest.2017.11

Abstract | Full Text

Epithelial–mesenchymal transition promotes SOX2 and NANOG expression in bladder cancer

Epithelial-mesenchymal transition (EMT) has been suggested as a potential cancer stemness trigger. The authors found that TGF-β induced-EMT increased SOX2 and Nanog protein levels in bladder cancer cells. Immunohistochemistry showed a mutually exclusive expression of E-cadherin and SOX2/Nanog. These findings suggest that EMT promotes stemness of bladder cancer, contributing to tumor aggressiveness.

Toshiro Migita, Ayano Ueda, Tomokazu Ohishi, Megumi Hatano, Hiroyuki Seimiya, Shin-ichiro Horiguchi, Fumitaka Koga and Futoshi Shibasaki

2017 97: 567-576; advance online publication, February 27, 2017; 10.1038/labinvest.2017.17

Abstract | Full Text


Suppression of islet homeostasis protein thwarts diabetes mellitus progression

Islet homeostasis protein (IHoP) co-localizes within pancreatic a-cells and is over-expressed in the islets of post-onset non-obese diabetic (NOD) mice and Type-1 diabetic patients. Suppression of IHoP during pre-diabetes altered the immune response producing normoglycemia in NOD mice for 35 weeks. IHoP levels increase in diabetic serum, indicating possible application as a biomarker.

Seh-Hoon Oh, Marda L Jorgensen, Clive H Wasserfall, Altin Gjymishka and Bryon E Petersen

2017 97: 577-590; advance online publication, February 20, 2017; 10.1038/labinvest.2017.15

Abstract | Full Text


Increased expression of latent TGF-β-binding protein 4 affects the fibrotic process in scleroderma by TGF-β/SMAD signaling

Scleroderma is a fibrosis-related disorder in which TGF-β plays a central role and latent TGF-β binding protein 4 (LTBP-4) affects the secretion of latent TGF-β to the extracellular matrix. Increased expression of LTBP-4 is found in serum and skin tissues of scleroderma patients, and its knockdown downregulates the secretion of TGF-β and activation of TGF-β/SMAD signaling. LTBP-4 plays roles in scleroderma by regulating TGF-β secretion.

Jiaying Lu, Qingmei Liu, Lei Wang, Wenzhen Tu, Haiyan Chu, Weifeng Ding, Shuai Jiang, Yanyun Ma, Xiangguang Shi, Weilin Pu, Xiaodong Zhou, Li Jin, Jiucun Wang and Wenyu Wu

2017 97: 591-601; advance online publication, March 6, 2017; 10.1038/labinvest.2017.20

Abstract | Full Text


Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation

Dengue virus (DENV) enhances degranulation of mast cells and production of chemokines (MCP-1, RANTES, and IP-10) in a mouse model. Anti-modified DENV NS1 (DJ NS1) antibodies reduce mast cell degranulation and production of DENV-induced chemokines. Therefore, antibodies against NS1 protein provide therapeutic benefits, which involve modulating DENV-induced mast cell activation.

Ya-Ting Chu, Shu-Wen Wan, Yu-Chang Chang, Chien-Kuo Lee, Betty A Wu-Hsieh, Robert Anderson and Yee-Shin Lin

2017 97: 602-614; advance online publication, February 27, 2017; 10.1038/labinvest.2017.10

Abstract | Full Text

Technical Report



Blue intensity matters for cell cycle profiling in fluorescence DAPI-stained images

Cell cycle deregulation is associated to several human pathologies, including cancer. The authors developed a method of image analysis based on DAPI-staining which allows the identification of the cell cycle momentum of individual and population of cells. Overall, the imaging framework exhibited a sensitivity of 94.0% and a specificity of 93.9% in discretizing the cell cycle phases of unperturbed, digitized cells.

Anabela Ferro, Tânia Mestre, Patrícia Carneiro, Ivan Sahumbaiev, Raquel Seruca and João M Sanches

2017 97: 615-625; advance online publication, March 6, 2017; 10.1038/labinvest.2017.13

Abstract | Full Text

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