Wako is has developed an exosome isolation kit using a novel affinity molecule. High yield high purity exosomes can be isolated by PS affinity method. Exosomes can be recovered from culture media containing FBS.
Nikon is offering special pricing on our award winning Eclipse Ts2 inverted tissue culture microscope with phase contrast optics. The Ts2 features intuitive controls, a compact body, LED illumination, and smart accessories such as the Contrast Shield which allows fluorescent samples to be easily observed in brightly lit labs. For more information, go to www.nikoninstruments.com/ts2-promo
Massively multiplex single-cell Hi-C pp263 - 266 Vijay Ramani, Xinxian Deng, Ruolan Qiu, Kevin L Gunderson, Frank J Steemers et al. doi:10.1038/nmeth.4155 Single-cell combinatorial indexed Hi-C (sciHi-C) is a streamlined protocol for generating thousands of high-quality single-cell chromosome conformation data sets that resemble bulk Hi-C data in aggregate.
Optogenetic inhibition of behavior with anion channelrhodopsins pp271 - 274 Farhan Mohammad, James C Stewart, Stanislav Ott, Katarina Chlebikova, Jia Yi Chua et al. doi:10.1038/nmeth.4148 Anion channelrhodopsins are light-sensitive chloride channels that can be used as optogenetic inhibitors. Mohammad et al. report their application in Drosophila, showing that various behaviors can be inhibited in a light-dependent manner.
Characterizing cell subsets using marker enrichment modeling pp275 - 278 Kirsten E Diggins, Allison R Greenplate, Nalin Leelatian, Cara E Wogsland and Jonathan M Irish doi:10.1038/nmeth.4149 Marker enrichment modeling (MEM) provides an objective metric for characterizing cell populations from high-content single-cell analysis. The MEM score outperforms standard metrics and provides a machine-readeable label for cell subsets.
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