Tuesday, February 14, 2017

Nature Methods Application Notes e-UPDATE: 14 February 2017

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14 February 2017 
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Robotic Microscopy with the Nikon Ti2 for high-content analysis applications
www.nikon.com >
Robotic Microscopy—a combination of high-content screening methods—enables multivariate experimental approaches with large cell populations and member-level sensitivity. Here we explore how the new Nikon Ti2 line of inverted research microscopes is uniquely suited to Robotic Microscopy applications, focusing on work utilizing induced pluripotent stem cells (iPSCs) as disease models in drug screening.

The Fast mode for ZEISS LSM 880 with Airyscan: highspeed confocal imaging
www.zeiss.com >
In May 2016, ZEISS announced the next innovation step for laser-scanning microscopy imaging with the introduction of the Fast mode for ZEISS LSM 880 with Airyscan. The new concept combines the Airyscan pinhole-plane detection technology with a new illumination shaping approach, enabling a fourfold increase in image acquisition rates. With the new Fast mode, Airyscan now affords researchers simultaneous access to super-resolution, increased signal-to-noise ratio and increased acquisition speeds without compromise.

DNA fragmentation and quality control analysis using Diagenode shearing systems and Fragment Analyzer
www.diagenode.com >
www.aati-us.com >
Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, namely, precise DNA fragmentation, size-range analysis, and smear quantification. The Diagenode One, Bioruptor®, Megaruptor®, and Fragment Analyzer™ by Advanced Analytical ensure that these first critical steps generate quality results.

Advances in Flavivirus research applications: new techniques using the FastPrep-24 5G sample prep system
www.mpbio.com >
The FastPrep-24 5G is the most innovative bead-beating sample-preparation instrument and produces quantitative, thorough and rapid grinding, lysis and homogenization of routine and resistant samples.

Automated NGS library preparation on CyBi-FeliX
www.cybio-ag.com >
Automation of library preparation is very important to reduce opportunities for error, increase reproducibility, and reduce the amount ofhands-on time allowing researchers to generate sequence data from DNA more efficiently. By utilizing the CyBi®-FeliX, a benchtop instrument for a variety of automated liquid handling tasks, the HaloPlex Target EnrichmentSystem Kit for NGS library preparation for Ion Torrent™ Sequencing was automated to achieve reliable final prepared libraries with high accuracy for downstream sequencing. This application note describes the successful proof of principle to generate high-quality DNA libraries on CyBi®-FeliX for personal genome analysis with the Ion Torrent™ Sequencing System.

MAV-seq: Management, Analysis and Visualization of Sequence Data
www.jax.org >
The increasing amount of heterogeneous Next Generation Sequencing (NGS) data generated today necessitates a robust platform for the efficient data storage, management, pre-processing, quality checking, analysis and visualization. Addressing each of these categories is a tremendous undertaking requiring high-level expertise from various disciplines. The typical genomic research laboratories do not always posses these resources, thus, we have developed a scientific solution to assist researchers dealing with the practical issues associated to the high throughput genomic data. We present MAV-seq as a robust platform, which facilitates the management of samples metadata and NGS data pre-processing, quality assessment and visualization. MAV-seq provides interactive modular data flow, which transparently integrates interoperability, structure, standardization, security, controlled accessibility, management of genomic studies and their sample metadata associated with datasets of enormous size and diversity.

A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources
www.bioind.com >
Human mesenchymal stem cells (hMSCs) are multipotent adult stem cells that can be isolated from various tissues as well as generated in vitro from hESCs and iPS cells. hMSCs have at least trilineage differentiation potential in vitro (into fat, bone, and cartilage cells), and it is part of the Minimal Experimental Criteria for MSC proposed by the International Society for Cellular Therapy (ISCT). In addition, differentiation of hMSCs into specific lineage provides the basis for the use of human MSC in cell therapy applications. Either undifferentiated or differentiated, hMSCs can be used for in vivo implantation into damaged tissue sites. The differentiation potential may differ in relation to the culture condition and the source of hMSCs, and it is still unknown which source should be used for each specific disease.


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