Nature Cell Biology contents: October 2014 Volume 16 Number 10, pp 919 - 1027

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Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement Sign up for the quarterly EMBL Events Newsletter Register to receive the latest information on courses and conferences taking place at EMBL Heidelberg and its outstations in Hinxton (United Kingdom), Grenoble (France), Hamburg (Germany) and Monterotondo (Italy). Sneak Preview of the 2015 events from the latest issue of our E-Newsletter.   Advertisement Nature Communications soon to be fully open access   From 20th October Nature Communications, will become fully open access for all new submissions. If an author has a manuscript they wish to submit to the journal via the subscription route, they must submit before 20th October. After this date all new submissions, if accepted, will be published open access and an article processing charge (APC) will apply. For any questions on the switch, open access or advice on policies and funding, visit our website, read the press release or contact our dedicated support team at openaccess@nature.com.   Review Top Single-cell technologies sharpen up mammalian stem cell research   pp919 - 927 Philipp S. Hoppe, Daniel L. Coutu and Timm Schroeder doi:10.1038/ncb3042 Schroeder and colleagues review the development and application of single-cell technologies — from gene and protein expression to clonal labelling, lineage tracing and time-lapse imaging — in stem cell research. News and Views Top Podosome rosettes precede vascular sprouts in tumour angiogenesis   pp928 - 930 Carmen M. Warren and M. Luisa Iruela-Arispe doi:10.1038/ncb3044 Expansion of a vascular network requires breaking through the basement membrane, a highly crosslinked barrier that tightly adheres to mature vessels. Angiogenic endothelial cells are now shown to form podosome rosettes that are able to focally degrade the extracellular matrix, prior to vascular sprouting in tumour angiogenesis. See also: Article by Seano et al. Cell Biology JOBS of the week Laboratory Research Assistant in Stem Cell Biology Icahn School of Medicine at Mount Sinai Postdoctoral Fellow, Cancer and Stem Cell Biology University of Pennsylvania Faculty Position in Developmental and Cell Biology The Stowers Institute for Medical Research Post Doctoral Fellow Istanbul University Institute of Experimental Medicine Postdoctoral Fellow Stony Brook University More Science jobs from Cell Biology EVENT Cell Biology of the Heart: Beyond the Myocyte-Centric View 01.03.15 Copper Mountain, USA More science events from Advertisement Fluorescence is at your fingertips with ZOE™, a benchtop cell imager you control by touch screen. Features brightfield illumination and three fluorescent channels. Step out of the darkroom, see the light!   Articles Top Endothelial podosome rosettes regulate vascular branching in tumour angiogenesis   pp931 - 941 Giorgio Seano, Giulia Chiaverina, Paolo Armando Gagliardi, Laura di Blasio, Alberto Puliafito et al. doi:10.1038/ncb3036 Seano, Primo and colleagues report that blood vessel branching during tumour angiogenesis is mediated by the formation of podosome rosettes that depends on VEGF-A and integrin α6β1. See also: News and Views by Warren & Iruela-Arispe Mammary stem cells have myoepithelial cell properties   pp942 - 950 Michael D. Prater, Valérie Petit, I. Alasdair Russell, Rajshekhar R. Giraddi, Mona Shehata et al. doi:10.1038/ncb3025 Myoepithelial cells considered terminally differentiated are now shown by Stingl and colleagues to have stem cell properties in vitro, to repopulate a mammary gland on transplantation and to behave as stem cells by lineage tracing. Single luminal epithelial progenitors can generate prostate organoids in culture   pp951 - 961 Chee Wai Chua, Maho Shibata, Ming Lei, Roxanne Toivanen, LaMont J. Barlow et al. doi:10.1038/ncb3047 Shen and colleagues report the in vitro generation of organoids from mouse luminal epithelial progenitor cells and normal or transformed prostate tissue, and extend this approach to the formation of normal and tumour organoids of human origin RASSF1A–LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2   pp962 - 971 Dafni-Eleftheria Pefani, Robert Latusek, Isabel Pires, Anna M. Grawenda, Karen S. Yee et al. doi:10.1038/ncb3035 O’Neill and colleagues find that the Hippo kinase LATS1 is part of an ATR-dependent response to stalled replication forks and protects RAD51 nucleofilaments on single strand DNA. EGFR has a tumour-promoting role in liver macrophages during hepatocellular carcinoma formation   pp972 - 981 Hanane Lanaya, Anuradha Natarajan, Karin Komposch, Liang Li, Nicole Amberg et al. doi:10.1038/ncb3031 Sibilia and colleagues report that IL-1-dependent EGFR induction in liver macrophages is needed to stimulate IL-6 production, which in turn promotes hepatocyte proliferation and hepatocellular carcinoma formation. Bladder cancers arise from distinct urothelial sub-populations   pp982 - 991 Jason Van Batavia, Tammer Yamany, Andrei Molotkov, Hanbin Dan, Mahesh Mansukhani et al. doi:10.1038/ncb3038 Mendelsohn and colleagues use lineage tracing in a mouse model of bladder cancer to show that different progenitor cell populations give rise to distinct types of urothelial and squamous cell carcinomas. PGC-1α mediates mitochondrial biogenesis and oxidative phosphorylation in cancer cells to promote metastasis   pp992 - 1003 Valerie S. LeBleu, Joyce T. O’Connell, Karina N. Gonzalez Herrera, Harriet Wikman, Klaus Pantel et al. doi:10.1038/ncb3039 Kalluri and colleagues find that mitochondrial biogenesis and respiration induced by transcriptional coactivator PGC-1α in cancer cells promote cancer metastasis and that PGC-1α expression is associated with invasive breast cancer. ΔNp63 promotes stem cell activity in mammary gland development and basal-like breast cancer by enhancing Fzd7 expression and Wnt signalling   pp1004 - 1015 Rumela Chakrabarti, Yong Wei, Julie Hwang, Xiang Hang, Mario Andres Blanco et al. doi:10.1038/ncb3040 Kang and colleagues demonstrate that the ΔNp63 isoform of p63 upregulates the expression of the Fzd7 Wnt receptor to promote normal and cancer stem cell activity in the mammary gland. Resource Top Systematic characterization of deubiquitylating enzymes for roles in maintaining genome integrity   pp1016 - 1026 Ryotaro Nishi, Paul Wijnhoven, Carlos le Sage, Jorrit Tjeertes, Yaron Galanty et al. doi:10.1038/ncb3028 Systematic characterization of deubiquitylating enzymes in the DNA-damage response identifies UCHL5 as promoting DNA-end resection. Corrigendum Top Corrigendum: Cyclin B2 and p53 control proper timing of centrosome separation   p1027 Hyun-Ja Nam and Jan M. van Deursen doi:10.1038/ncb3049 Top Advertisement Call for Papers Nature Plants: From Bench to Biosphere Nature Plants is now open for submissions and welcomes manuscripts from all areas of plant research. Submit your research online today!  View the complete Guide to Authors.     Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. 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