Nature Cell Biology contents: August 2012 Volume 14 Number 8, pp 775 - 890

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Flow cytometry within reach™.  bdbiosciences.com/go/accuri Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement Feel like your paper is lost in a sea of submissions? Scientific Reports publishes your research quickly and efficiently, and makes it freely available to the global scientific community. Learn more with our one minute illustrative video.   Editorial Top The data deluge   p775 doi:10.1038/ncb2558 The Royal Society calls for a shift in the attitude of scientists and others, including funders, research institutions and publishers, towards data accessibility, curation and dissemination. Turning Points Top Finding CDK: Linking yeast with humans   p776 Paul Nurse doi:10.1038/ncb2547 Review Top Classifying collective cancer cell invasion   pp777 - 783 Peter Friedl, Joseph Locker, Erik Sahai and Jeffrey E. Segall doi:10.1038/ncb2548 Most invasive solid tumours display predominantly collective invasion, in which groups of cells invade the peritumoral stroma while maintaining cell-cell contacts. As the concepts and experimental models for functional analysis of collective cancer cell invasion are rapidly developing, we propose a framework for addressing potential mechanisms, experimental strategies and technical challenges to study this process. News and Views Top Nuanced junctional RhoA activity   pp784 - 786 Swapnil S. Kher and Rebecca A. Worthylake doi:10.1038/ncb2553 RhoA signalling controls many diverse cellular processes, and thus discovering the mechanisms that determine its specific outcomes is a tantalizing challenge. A previously uncharacterized regulatory module operates selectively at the zonula adherens of epithelial cell junctions, in which positive and negative RhoA regulators are coordinated to fine-tune RhoA activity. See also: Article by Ratheesh et al. FOXOphagy path to inducing stress resistance and cell survival   pp786 - 788 Marco Sandri doi:10.1038/ncb2550 Nutrient deprivation and other stress stimuli elicit metabolic changes (such as the induction of autophagy and activation of FOXO transcription factors) that help an organism adapt to stressful conditions. A link between these stress response pathways is revealed by the finding that FOXO3 upregulates the expression of glutamine synthetase to promote glutamine accumulation, inhibit mTOR signalling and promote autophagy. See also: Article by van der Vos et al. Brassinosteroids, gibberellins and light-mediated signalling are the three-way controls of plant sprouting   pp788 - 790 Yvon Jaillais and Grégory Vert doi:10.1038/ncb2551 The steroid hormones found in plants, the brassinosteroids, were originally genetically identified about 15 years ago as critical regulators of seedling photomorphogenesis. Two studies now shed light on the molecular mechanisms behind this observation. Brassinosteroids control seedling morphogenesis through direct interaction with master transcriptional regulators downstream of growth-promoting hormones and light signalling. See also: Article by Oh et al. | Article by Bai et al. Seeing the Warburg effect in the developing retina   pp790 - 791 Brian P. Fiske and Matthew G. Vander Heiden doi:10.1038/ncb2554 Proliferating cells of the Xenopus laevis retina facultatively use aerobic glycolysis instead of oxidative phosphorylation. This demonstrates that the metabolic rewiring usually associated with the Warburg effect in tumorigenesis may be a more widespread feature of proliferative metabolism than generally appreciated. See also: Letter by Agathocleous et al. Cell Biology JOBS of the week Senior Researcher and Head of Section for Bacteriology, Pathology and Parasitology Technical University of Denmark (DTU) Bioinformatician, Department of Oncology-Pathology Karolinska Instituet Post-doctoral position: Metabolic defects in cancer Inserm U970 Post-doctoral / Engineering position at the interface between cell biology and physics CNRS (Centre National de la Recherche Scientifique, France) International PhD Program Molecular Cell Biology, Berlin Germany MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE (MDC) BERLIN-BUCH More Science jobs from Cell Biology EVENT Successful cell analysis � improve the basic steps of every experiment 29.-30.08.12 Germany More science events from Research Highlights Top Stem and cancer cells Wnt long telomeres | Watching hair grow | BRCA2 in abscission | Synchronizing actin and microtubules for axonal branching Articles Top Rap2A links intestinal cell polarity to brush border formation   pp793 - 801 Martijn Gloerich, Jean Paul ten Klooster, Marjolein J. Vliem, Thijs Koorman, Fried J. Zwartkruis, Hans Clevers and Johannes L. Bos doi:10.1038/ncb2537 Microvilli are essential for the function of intestinal cells. Bos and colleagues have found that the polarity kinase LKB-1 induces PtdIns(4,5)P2 enrichment at the apical membrane. This leads to the successive accumulation of phosphatidic acid and the small GTPase Rap2A with its GEF and its effectors. These, in turn, trigger the changes in the actin cytoskeleton responsible for microvilli formation. Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses   pp802 - 809 Eunkyoo Oh, Jia-Ying Zhu and Zhi-Yong Wang doi:10.1038/ncb2545 Wang and colleagues have uncovered a direct functional relationship between the brassinosteroid-activated transcription factor BZR1 and the light- and heat-sensitive transcription factor PIF4. This interplay integrates hormonal and environmental signals to modulate cell elongation during plant growth. See also: News and Views by Jaillais & Vert Brassinosteroid, gibberellin and phytochrome impinge on a common transcription module in Arabidopsis    pp810 - 817 Ming-Yi Bai, Jian-Xiu Shang, Eunkyoo Oh, Min Fan, Yang Bai, Rodolfo Zentella, Tai-ping Sun and Zhi-Yong Wang doi:10.1038/ncb2546 Wang and colleagues show that, in Arabidopsis thaliana, brassinosteroid and light-dependent transcription factors are required for giberellin effects on hypocotyl elongation, by modulating transcription of giberellin-induced genes involved in cell wall synthesis and photosynthesis. Conversely, giberellin relieves the brassinosteroid component BZR1 from inhibition by DELLA proteins. See also: News and Views by Jaillais & Vert Centralspindlin and α-catenin regulate Rho signalling at the epithelial zonula adherens   pp818 - 828 Aparna Ratheesh, Guillermo A. Gomez, Rashmi Priya, Suzie Verma, Eva M. Kovacs, Kai Jiang, Nicholas H. Brown, Anna Akhmanova, Samantha J. Stehbens and Alpha S. Yap doi:10.1038/ncb2532 Yap and colleagues identify an extramitotic role for the centralspindlin complex, a Rho signalling regulator during cytokinesis. They show that centralspindlin interacts with α-catenin, and promotes epithelial junctional integrity by recruiting the ECT2 RhoGEF to the zonula adherens, as well as by inhibiting the junctional localization of the p190 B RhoGAP. See also: News and Views by Kher & Worthylake Modulation of glutamine metabolism by the PI(3)K–PKB–FOXO network regulates autophagy   pp829 - 837 Kristan E. van der Vos, Pernilla Eliasson, Tassula Proikas-Cezanne, Stephin J. Vervoort, Ruben van Boxtel, Marrit Putker, Iris J. van Zutphen, Mario Mauthe, Sebastian Zellmer, Cornelieke Pals, Liesbeth P. Verhagen, Marian J. A. Groot Koerkamp, A. Koen Braat, Tobias B. Dansen, Frank C. Holstege, Rolf Gebhardt, Boudewijn M. Burgering and Paul J. Coffer doi:10.1038/ncb2536 Coffer and colleagues report that the transcription factor FOXO3 regulates the induction of autophagy. In response to PI(3)K–Akt signalling, FOXO3 directly induces expression of glutamine synthase, which upregulates glutamine levels and triggers autophagy. See also: News and Views by Sandri Synaptotagmin-like proteins control the formation of a single apical membrane domain in epithelial cells   pp838 - 849 Manuel Gálvez-Santisteban, Alejo E. Rodriguez-Fraticelli, David M. Bryant, Silvia Vergarajauregui, Takao Yasuda, Inmaculada Bañón-Rodríguez, Ilenia Bernascone, Anirban Datta, Natalie Spivak, Kitty Young, Christiaan L. Slim, Paul R. Brakeman, Mitsunori Fukuda, Keith E. Mostov and Fernando Martín-Belmonte doi:10.1038/ncb2541 By performing a screen for genes that regulate epithelial architecture, Martín–Belmonte and colleagues identify key roles for the synaptotagmin-like proteins Slp2-a and Slp4-a in restricting lumen generation. They find that Slp2-a targets Rab27a/b-positive vesicles to PtdIns(4,5)P2-enriched apical membranes, whereas Slp4-a controls subsequent vesicle tethering and fusion. Their coordinated activities ensure the creation of a single lumen per cell. Direct inhibition of the cold-activated TRPM8 ion channel by Gαq    pp851 - 858 Xuming Zhang, Stephanie Mak, Lin Li, Andres Parra, Bristol Denlinger, Carlos Belmonte and Peter A. McNaughton doi:10.1038/ncb2529 Zhang, McNaughton and colleagues show that inflammatory stimuli promote a direct association between the heterotrimeric G-protein subunit Gαq and the temperature-sensitive ion channel TRPM8. This interaction inhibits TRPM8 channel activity, providing a mechanism by which inflammation produces aberrant sensations of temperature changes. Letters Top Metabolic differentiation in the embryonic retina   pp859 - 864 Michalis Agathocleous, Nicola K. Love, Owen Randlett, Julia J. Harris, Jinyue Liu, Andrew J. Murray and William A. Harris doi:10.1038/ncb2531 It is unclear whether proliferating and differentiating cells produce energy through different metabolic pathways. Harris and colleagues show, in the embryonic Xenopus retina, that dividing progenitors use glycogen for glycolysis, and that a transition to oxidative phosphorylation occurs as cells differentiate. See also: News and Views by Fiske & Vander Heiden Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment   pp865 - 873 Jayachandran Gopalakrishnan, Yiu-Cheung Frederick Chim, Andrew Ha, Marcus L. Basiri, Dorothy A. Lerit, Nasser M. Rusan and Tomer Avidor-Reiss doi:10.1038/ncb2527 Avidor-Reiss and colleagues show that the nucleotide status of tubulin regulates recruitment of pericentriolar material. Binding of GTP-bound tubulin to the Sas-4 centrosomal protein prevents the Sas-4-dependent formation of centrosomal protein complexes, whereas the Sas-4-stimulated hydrolysis of tubulin-GTP into tubulin-GDP has the opposite effect. External push and internal pull forces recruit curvature-sensing N-BAR domain proteins to the plasma membrane   pp874 - 881 Milos Galic, Sangmoo Jeong, Feng-Chiao Tsai, Lydia-Marie Joubert, Yi I. Wu, Klaus M. Hahn, Yi Cui and Tobias Meyer doi:10.1038/ncb2533 N-BAR-domain-containing proteins regulate membrane dynamics, as they stabilize curved membrane topologies, but whether they primarily sense or generate curvature has remained unclear. Galic, Meyer and colleagues now report that N-BAR proteins accumulate at highly curved membrane areas. Sox10 promotes the formation and maintenance of giant congenital naevi and melanoma   pp882 - 890 Olga Shakhova, Daniel Zingg, Simon M. Schaefer, Lisette Hari, Gianluca Civenni, Jacqueline Blunschi, Stéphanie Claudinot, Michal Okoniewski, Friedrich Beermann, Daniela Mihic-Probst, Holger Moch, Michael Wegner, Reinhard Dummer, Yann Barrandon, Paolo Cinelli and Lukas Sommer doi:10.1038/ncb2535 Shakhova, Sommer and colleagues use mouse models to demonstrate that the Sox10 transcription factor is crucial for the formation and maintenance of giant congenital naevi and melanoma. They show, in human melanoma cells, that Sox10 promotes neural crest stem cell properties, cell proliferation and cell survival. Addendum Top Patchwork organization of the yeast plasma membrane into numerous coexisting domains   p890 Felix Spira, Nikola S. Mueller, Gisela Beck, Philipp von Olshausen, Joachim Beig and Roland Wedlich-Söldner doi:10.1038/ncb2552 Top Advertisement Nurture your career with the new-look Naturejobs As the world's largest dedicated jobs boards for the scientific community, Naturejobs is the ideal resource to build your career. Our new and improved functionality enables you to search, save and apply for jobs quickly and easily. Search over 10,000 vacancies to find exactly the right job for you at: www.naturejobs.com.   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. 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