The EMBO Journal - Table of Contents alert Volume 31 Issue 9

TABLE OF CONTENTS 02 May 2012 | Volume 31, Issue 9 Have you seen? Review Articles Also new AOP Advertisement Reliable quality control for any RNA sample The exceptionally versatile 2100 Bioanalyzer system, employing the RNA Integrity Number (RIN) algorithm, is the industry standard for RNA quality assessment. It lets you reliably assess the integrity and concentration of any RNA sample you encounter –including small RNA and plant RNA– helping you make the most of your valuable time and resources. View latest webinar to learn more. Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement EMBO publications survey How do you search the scientific literature? How should we ‘embed’ data and supplementary information in papers? Please, take a few minutes to complete our anonymous ONLINE SURVEY: www.surveymonkey.com/s/embo-data-search EMBO is striving to improve the quality and depth of the scientific information included in published papers and your input will guide important developments at our journals.   Have you seen? Top The many functions of cohesin—different rings to rule them all? Two studies generating and characterizing mice that lack the core cohesin subunit SA1 reveal its essential roles in telomere replication and gene expression, which cannot be taken over by cohesin complexes containing the paralog SA2. Jan-Michael Peters The EMBO Journal (2012), 31, 2061 - 2063; 10.1038/emboj.2012.90 Abstract | Full text | PDF Published online: 10 April 2012 Lrig1: a new master regulator of epithelial stem cells Work from Kim Jensen's lab recently published in NCB establishes the crucial role of Lrig1 in the control of intestinal homeostasis. Paloma Ordóñez-Morán and Joerg Huelsken The EMBO Journal (2012), 31, 2064 - 2066; 10.1038/emboj.2012.73 Abstract | Full text | PDF Published online: 20 March 2012 Review Top Mechanisms regulating epidermal stem cells This review synthesizes recently published novel insights into the biological function and homeostatic control of epidermal stem cells. Benjamin Beck and Cédric Blanpain The EMBO Journal (2012), 31, 2067 - 2075; 10.1038/emboj.2012.67 Abstract | Full text | PDF Published online: 20 March 2012 Subject Categories: Development Articles Top Cohesin-SA1 deficiency drives aneuploidy and tumourigenesis in mice due to impaired replication of telomeres Mice lacking the cohesin subunit SA1 reveal a non-redundant chromosome segregation role independent of centromeric cohesion maintenance. In contrast to the paralog SA2, whose inactivation in cancers promotes aneuploidy via bona fide cohesion defects, SA1-mediated cohesion is required for accurate telomere replication and stabilization. Silvia Remeseiro, Ana Cuadrado, María Carretero, Paula Martínez, William C Drosopoulos, Marta Cañamero, Carl L Schildkraut, María A Blasco and Ana Losada The EMBO Journal (2012), 31, 2076 - 2089; 10.1038/emboj.2012.11 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 March 2012 Subject Categories: Cell Cycle | Genome Stability and Dynamics A unique role of cohesin-SA1 in gene regulation and development Loss of the cohesin subunit SA1 causes developmental defects and transcriptional deregulation resembling familial cohesin dysfunction disorders such as CdLS. SA1-containing complexes are enriched at insulator and promoter regions, suggesting gene expression control specifically by SA1-, not SA2-cohesin. Silvia Remeseiro, Ana Cuadrado, Gonzalo Gómez-López, David G Pisano and Ana Losada The EMBO Journal (2012), 31, 2090 - 2102; 10.1038/emboj.2012.60 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 March 2012 Subject Categories: Chromatin and Transcription | Cell Cycle HIF1α induced switch from bivalent to exclusively glycolytic metabolism during ESC-to-EpiSC/hESC transition Metabolic analyses on naive ESCs versus primed EpiSCs reveal a functional switch from oxidative phosphorylation to glycolysis. Differential gene expression defines HIF1α and reduced complex IV activity as crucial metabolic regulators during stem-cell differentiation. Wenyu Zhou, Michael Choi, Daciana Margineantu, Lilyana Margaretha, Jennifer Hesson, Christopher Cavanaugh, C Anthony Blau, Marshall S Horwitz, David Hockenbery, Carol Ware and Hannele Ruohola-Baker The EMBO Journal (2012), 31, 2103 - 2116; 10.1038/emboj.2012.71 Abstract | Full text | PDF | Supp. info. Published online: 23 March 2012 Subject Categories: Development | Cellular Metabolism Loss of mitochondrial protease OMA1 alters processing of the GTPase OPA1 and causes obesity and defective thermogenesis in mice The metalloprotease OMA1 is involved in the proteolytic inactivation of the dynaminrelated GTPase OPA1, a key regulator of mitochondrial dynamics. OMA1 knockout mice indicate that mitochondrial quality control and dynamics impact energy metabolism, in particular under metabolic stress. Pedro M Quirós, Andrew J Ramsay, David Sala, Erika Fernández-Vizarra, Francisco Rodríguez, Juan R Peinado, Maria Soledad Fernández-García, José A Vega, José A Enríquez, Antonio Zorzano and Carlos López-Otín The EMBO Journal (2012), 31, 2117 - 2133; 10.1038/emboj.2012.70 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 20 March 2012 Subject Categories: Proteins | Cellular Metabolism Tightening of the ATP-binding sites induces the opening of P2X receptor channels P2X receptors are ATP-gated ion channels involved in many processes, including synaptic transmission and sensory signalling, but their gating mechanism is still ill understood. This study reveals that extracellular ATP opens the channel by tightening nucleotide-binding sites that are shaped like open ‘jaws’ Ruotian Jiang, Antoine Taly, Damien Lemoine, Adeline Martz, Olivier Cunrath and Thomas Grutter The EMBO Journal (2012), 31, 2134 - 2143; 10.1038/emboj.2012.75 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 30 March 2012 Subject Categories: Membranes and Transport | Structural Biology Solution single-vesicle assay reveals PIP2-mediated sequential actions of synaptotagmin-1 on SNAREs Synaptotagmin-1 (Syt1) is a major calcium sensor required for SNARE-mediated synaptic vesicle fusion. ALEX, a single-molecule technique capable of characterizing subpopulations of fusion intermediates, reveals distinct roles of Syt1 in vesicle docking and fusion with the plasma membrane. Jae-Yeol Kim, Bong-Kyu Choi, Mal-Gi Choi, Sun-Ae Kim, Ying Lai, Yeon-Kyun Shin and Nam Ki Lee The EMBO Journal (2012), 31, 2144 - 2155; 10.1038/emboj.2012.57 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 09 March 2012 Subject Categories: Membranes and Transport | Neuroscience Munc18-1 mutations that strongly impair SNARE-complex binding support normal synaptic transmission Synaptic vesicle exocytosis depends on the SM protein Munc18-1, but its precise role is controversial. Munc18-1 mutants reveal that the protein acts primarily before/during SNARE complex assembly rather than at the level of assembled fusion complexes. Marieke Meijer, Pawel Burkhardt, Heidi de Wit, Ruud F Toonen, Dirk Fasshauer and Matthijs Verhage The EMBO Journal (2012), 31, 2156 - 2168; 10.1038/emboj.2012.72 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 23 March 2012 Subject Categories: Membranes and Transport | Neuroscience Bat3 facilitates H3K79 dimethylation by DOT1L and promotes DNA damage-induced 53BP1 foci at G1/G2 cell-cycle phases The human DNA repair protein 53BP1 is targeted to sites of DNA damage by several histone methylation marks. Bat3 aids generation of H3K79 methyl marks outside of S-phase by recruiting the methyltransferase DOT1L through a ubiquitin-like domain. Timothy P Wakeman, Qinhong Wang, Junjie Feng and Xiao-Fan Wang The EMBO Journal (2012), 31, 2169 - 2181; 10.1038/emboj.2012.50 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 28 February 2012 Subject Categories: Chromatin and Transcription | Genome Stability and Dynamics Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components Acute removal of the fission yeast Mcm10 replication protein via an auxin-inducible degron shows that it activates DNA unwinding and initiation independent of assembling the Cdc45–Mcm2-7–GINS helicase complex. Mai Kanke, Yukako Kodama, Tatsuro S Takahashi, Takuro Nakagawa and Hisao Masukata The EMBO Journal (2012), 31, 2182 - 2194; 10.1038/emboj.2012.68 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 20 March 2012 Subject Categories: Genome Stability and Dynamics Mcm10 associates with the loaded DNA helicase at replication origins and defines a novel step in its activation The replication factor Mcm10 is required for activation of the Mcm2-7 replicative helicase loaded at replication origins, while its acute depletion does not affect helicase assembly or interaction with DNA polymerase alpha in budding yeast. Frederick van Deursen, Sugopa Sengupta, Giacomo De Piccoli, Alberto Sanchez-Diaz and Karim Labib The EMBO Journal (2012), 31, 2195 - 2206; 10.1038/emboj.2012.69 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 20 March 2012 Subject Categories: Genome Stability and Dynamics EBV and human microRNAs co-target oncogenic and apoptotic viral and human genes during latency The global analysis of miRNA targets in EBV transformed B-cells shows that viral miRNAs mostly target cellular and not viral genes, many of which are also targeted by the oncogenic human miR-17~92 cluster. Kasandra J Riley, Gabrielle S Rabinowitz, Therese A Yario, Joseph M Luna, Robert B Darnell and Joan A Steitz The EMBO Journal (2012), 31, 2207 - 2221; 10.1038/emboj.2012.63 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 30 March 2012 Subject Categories: RNA | Microbiology and Pathogens Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein Splicing factor Cwc2 positions pre-mRNA and U6 RNA during the activation of the spliceosome. The structure of Cwc2 and mapping of the Cwc2–U6 snRNA interaction provide insight into the spliceosome catalytic centre. Jana Schmitzová, Nicolas Rasche, Olexander Dybkov, Katharina Kramer, Patrizia Fabrizio, Henning Urlaub, Reinhard Lührmann and Vladimir Pena The EMBO Journal (2012), 31, 2222 - 2234; 10.1038/emboj.2012.58 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 09 March 2012 Subject Categories: RNA | Structural Biology Advertisement BEATSON INTERNATIONAL CANCER CONFERENCE Membrane Dynamics in Cancer 1 - 4 July 2012 Glasgow University The aim of the conference is to discuss the role of membrane transport in cellular processes such as autophagy, cell migration, receptor signalling, lysosome exocytosis and exosome release in the context of their effects on tumour growth, survival and metastasis www.beatson.gla.ac.uk/conf   Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to The EMBO Journal. In order to do so, please purchase a subscription. 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