The EMBO Journal - Table of Contents alert Volume 31 Issue 4

TABLE OF CONTENTS 15 February 2012 | Volume 31, Issue 4 Have you seen? Article Report Articles Also new AOP Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement Optimal NGS library construction at LIGHTNING speed! Create high-quality genomic libraries for any sequencing platform and achieve successful, cost-efficient next-generation sequencing experiments in 1/5th the time of competing systems. Diagenode's Bioruptor Sonicator is proven as the shearing device of choice providing optimal yields, quality, and fragment size at the industry''s best value. Learn how Diagenode will maximize your sequencing results. Have you seen? Top Lymphatics thrive on stress: mechanical force in lymphatic development Blood vessel development and homeostasis is fine-tuned by tissue demands. M Simons and M Schwartz comment on this issue's article by Dr Lammert and colleagues, in which they demonstrate that interstitial fluid mechanically induces growth of the lymphatic vasculature and, therefore, that a similar principle regulates lymphatic development. Martin A Schwartz and Michael Simons The EMBO Journal (2012), 31, 781 - 782; 10.1038/emboj.2011.484 Abstract | Full text | PDF Published online: 15 February 2012 Directional gating of synaptic plasticity by GPCRs and their distinct downstream signalling pathways The G-protein-coupled receptors PAC1R and D1R selectively affect the induction of long-term potentiation (LTP) and long-term depression (LTD) by differentially regulating NMDA receptor subtypes. Bo Gong and Yu Tian Wang The EMBO Journal (2012), 31, 783 - 785; 10.1038/emboj.2012.3 Abstract | Full text | PDF Published online: 15 February 2012 A microRNA that limits metastatic colonisation and endothelial recruitment Recent work from the Tavazoie-lab published in Nature reveals that miR-126 and its targets control the capability of cancer cells to recruit mature endothelial cells. This establishes miR-126 as non-cell autonomous determinant of metastatic progression. Yeesim Khew-Goodall and Gregory J Goodall The EMBO Journal (2012), 31, 786 - 787; 10.1038/emboj.2011.504 Abstract | Full text | PDF Published online: 10 January 2012 Articles Top Mechanoinduction of lymph vessel expansion Manipulating fluid pressure in the developing lymphatic system demonstrates that lymphatic endothelial cells are responsive to cell stretching, such that increased pressure promotes integrin-dependent VEGFR3 signalling, cell proliferation, and hence vessel growth. Lara Planas-Paz, Eckhard Lammert and colleagues The EMBO Journal (2012), 31, 788 - 804; 10.1038/emboj.2011.456 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 December 2011 Subject Categories: Cell and Tissue Architecture | Development Metaplasticity gated through differential regulation of GluN2A versus GluN2B receptors by Src family kinases Synaptic plasticity, which forms the basis of learning and memory, is controlled by GPCRs. GPCR signalling results in either long-term potentiation (LTP) or depression (LTD) by differentially regulating NMDA receptor subtypes via activation of distinct Src kinase family members. Kai Yang, John F MacDonald and colleagues The EMBO Journal (2012), 31, 805 - 816; 10.1038/emboj.2011.453 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 20 December 2011 Subject Categories: Signal Transduction | Neuroscience Pten coordinates retinal neurogenesis by regulating Notch signalling Mouse retinal progenitor cells lacking the phosphatase Pten complete neurogenesis prematurely due to inactivation of Notch signalling. Loss of Pten leads to enhanced PI3K-Akt signalling that interferes with the formation of Notch transcription activator complex. Hong Seok Jo, Kyung Hwa Kang, Cheol O Joe and Jin Woo Kim The EMBO Journal (2012), 31, 817 - 828; 10.1038/emboj.2011.443 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 06 December 2011 Subject Categories: Signal Transduction | Neuroscience CSPα knockout causes neurodegeneration by impairing SNAP-25 function The synaptic vesicle protein CSPα is a co-chaperone for the presynaptic SNARE protein SNAP-25 and regulates SNARE-complex assembly. Complementation experiments with CSPα knockout mice and SNAP-25 demonstrate that the destabilization of SNAP-25 alone is sufficient to cause neurodegeneration. Manu Sharma, Thomas C Südhof and colleagues The EMBO Journal (2012), 31, 829 - 841; 10.1038/emboj.2011.467 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 20 December 2011 Subject Categories: Membranes and Transport | Neuroscience Identification and characterization of a resident vascular stem/progenitor cell population in preexisting blood vessels The existence of vessel-resident endothelial stem cells remains a matter of debate. The results presented in this study support the existence of a CD31+/CD45− side population in mouse vascular endothelia that carry features of endothelial stem cells/progenitors. Hisamichi Naito, Nobuyuki Takakura and colleagues The EMBO Journal (2012), 31, 842 - 855; 10.1038/emboj.2011.465 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 16 December 2011 Subject Categories: Development | Molecular Biology of Disease A novel GRK2/HDAC6 interaction modulates cell spreading and motility The G protein-coupled receptor kinase (GRK2) regulates cell motility via MAPK-dependent modulation of focal adhesion turnover. Here, it is shown to regulate microtubule dynamics as well, phosphorylating HDAC6 and promoting tubulin deacetylation. Vanesa Lafarga, Ivette Aymerich, Olga Tapia, Federico Mayor Jr and Petronila Penela The EMBO Journal (2012), 31, 856 - 869; 10.1038/emboj.2011.466 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 23 December 2011 Subject Categories: Cell and Tissue Architecture | Signal Transduction dNTP pools determine fork progression and origin usage under replication stress Hydroxyurea or DNA damaging agents cause replication fork stalling and inhibition of further origin firing. The DNA damage response does not directly control replication origins, but rather regulates nucleotide levels, which determine fork speed as well as how long replication continues under stress. Jérôme Poli, Philippe Pasero and colleagues The EMBO Journal (2012), 31, 883 - 894; 10.1038/emboj.2011.470 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 10 January 2012 Subject Categories: Genome Stability and Dynamics Endogenous DNA replication stress results in expansion of dNTP pools and a mutator phenotype Mild DNA damage checkpoint activation in yeast is sufficient to cause increased nucleotide pools and subsequent mutagenesis, revealing basal levels of replication stress as potential source of genomic instability. Marta B Davidson, Grant W Brown and colleagues The EMBO Journal (2012), 31, 895 - 907; 10.1038/emboj.2011.485 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 10 January 2012 Subject Categories: Genome Stability and Dynamics Dysregulation of DNA polymerase κ recruitment to replication forks results in genomic instability The deubiquitinase USP1, which also targets Fanconi Anaemia pathway proteins, makes a major contribution to genome stability via the replication factor PCNA. PCNA deubiquitination prevents unscheduled recruitment of an error-prone translesion synthesis DNA polymerase and ensures replication fidelity. Mathew JK Jones, Luca Colnaghi and Tony T Huang The EMBO Journal (2012), 31, 908 - 918; 10.1038/emboj.2011.457 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 December 2011 Subject Categories: Genome Stability and Dynamics DNA binding to proteolytically activated TLR9 is sequence-independent and enhanced by DNA curvature Proteolytically processed Toll-like receptor 9 (TLR9) ectodomains recognize microbial DNA in endolysosomes, inducing an innate immune response. TLR9 ectodomains preferentially recognize curved DNA backbones, and ligand binding induces TLR9 dimerization in a sequence-independent manner. Yue Li, Ian C Berke and Yorgo Modis The EMBO Journal (2012), 31, 919 - 931; 10.1038/emboj.2011.441 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 06 December 2011 Subject Categories: Signal Transduction | Immunology Phagosomal proteolysis in dendritic cells is modulated by NADPH oxidase in a pH-independent manner The proteolytic activity of phagosomes is tightly regulated in dendritic cells, as this affects antigen processing. Here, the NADPH oxidase NOX2 is shown to control phagosomal cathepsin proteases in a ROS-dependent manner, but independently of phagosomal acidification. Joanna M Rybicka, Robin M Yates and colleagues The EMBO Journal (2012), 31, 932 - 944; 10.1038/emboj.2011.440 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 December 2011 Subject Categories: Immunology VprBP binds full-length RAG1 and is required for B-cell development and V(D)J recombination fidelity Here, the ubiquitin ligase Cul4A/DDB1/VprBP is implicated in RAG1-dependent DNA rearrangements in B cells. RAG1 recombinase binds directly to the VprBP subunit and stimulates ubiquitin ligase activity of the complex. Mice lacking VprBP show recombination defects and stalled B-cell development. Michele D Kassmeier, Patrick C Swanson and colleagues The EMBO Journal (2012), 31, 945 - 958; 10.1038/emboj.2011.455 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 December 2011 Subject Categories: Immunology CPEB2–eEF2 interaction impedes HIF-1α RNA translation The transcription factor HIF-1α plays an important role in the adaptation to hypoxic stress. Under normoxic conditions, the RNA-binding protein CPEB2 inhibits the ribosome-triggered GTPase activity of the translation elongation factor eEF2 and attenuates HIF-1α translation. Po-Jen Chen and Yi-Shuian Huang The EMBO Journal (2012), 31, 959 - 971; 10.1038/emboj.2011.448 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 09 December 2011 Subject Categories: RNA | Proteins The SNF2-like helicase HELLS mediates E2F3-dependent transcription and cellular transformation HELLS/Lsh, previously linked to heterochromatin formation and transcriptional repression, is identified as an E2F3 cofactor that aids expression of cell-cycle genes and oncogenic transformation. HELLS may act as chromatin remodeller, whose promoter binding position and activatory/repressive functions change depending on E2F3. Björn von Eyss, Ulrike Ziebold and colleagues The EMBO Journal (2012), 31, 972 - 985; 10.1038/emboj.2011.451 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 December 2011 Subject Categories: Chromatin and Transcription | Cell Cycle Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development The transcription factor c-Myb plays an important role in the differentiation of haematopoietic stem cells. This study describes the changes in the organization of the Myb gene locus during erythroid differentiation and uncovers a role of the intronic binding site for CTCF in the transition from transcriptional initiation to elongation. Ralph Stadhouders, Eric Soler and colleagues The EMBO Journal (2012), 31, 986 - 999; 10.1038/emboj.2011.450 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 December 2011 Subject Categories: Chromatin and Transcription | Development How a single residue in individual β-thymosin/WH2 domains controls their functions in actin assembly β-Thymosin/WH2 domain-containing proteins can either sequester actin monomers or promote filament assembly. These opposing functions are defined a single residue on their actin interaction interface, which determines the dynamics of actin binding. Dominique Didry, Louis Renault and colleagues The EMBO Journal (2012), 31, 1000 - 1013; 10.1038/emboj.2011.461 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 23 December 2011 Subject Categories: Cell and Tissue Architecture | Structural Biology Sec24p and Sec16p cooperate to regulate the GTP cycle of the COPII coat The GTP cycle driven by Sar1p and the COPII coat regulates vesicle budding at the endoplasmic reticulum. A novel regulatory complex consisting of Sec24p and Sec16p slows down GTP hydrolysis to prevent premature vesicle scission. Leslie F Kung, Elizabeth A Miller and colleagues The EMBO Journal (2012), 31, 1014 - 1027; 10.1038/emboj.2011.444 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 09 December 2011 Subject Categories: Membranes and Transport Rescue of tropomyosin deficiency in Drosophila and human cancer cells by synaptopodin reveals a role of tropomyosin α in RhoA stabilization Although structurally unrelated, the actin-binding proteins synaptopodin and tropomyosin are here found to have a common function in regulating stress fibre formation via blocking ubiquitination and degradation of RhoA. Jenny S Wong, Peter Mundel and colleagues The EMBO Journal (2012), 31, 1028 - 1040; 10.1038/emboj.2011.464 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 13 December 2011 Subject Categories: Cell and Tissue Architecture Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation The initiation of filament assembly in the type II secretion system remains poorly understood. During pseudopilus biogenesis in Klebsiella oxytoca, the pseudopilins PulI and PulJ self-assemble at the inner membrane; binding of PulK induces a conformational change that promotes pseudopilus polymerization and thus secretion of the pseudopilin PulG. David A Cisneros, Olivera Francetic and colleagues The EMBO Journal (2012), 31, 1041 - 1053; 10.1038/emboj.2011.454 Abstract | Full text | PDF | Supp. info. | Review Process File Published online: 09 December 2011 Subject Categories: Membranes and Transport | Microbiology and Pathogens Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to The EMBO Journal. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department. For print subscription enquiries, please contact our subscription department. For other enquiries, please contact our customer feedback department. Nature Publishing Group | 75 Varick Street, 9th Floor | New York | NY 10013-1917 | USA Nature Publishing Group's worldwide offices: London - Paris - Munich - New Delhi - Tokyo - Melbourne San Diego - San Francisco - Washington - New York - Boston Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at Brunel Road, Houndmills, Basingstoke, Hampshire RG21 6XS. © 2011 European Molecular Biology Organization