Nature Cell Biology contents: June 2016 Volume 18 Number 6, pp 579 - 709

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Advertisement Once Upon a Time...... there was a cell and seen nevermore again? CELLviewTM Slide stops meniscus and bleaching effects!   For the Cultivation, Staining and Microscopy of Cells - Detachable black compartmentalisation  - Round 96 well microtiter plate design - Reduced meniscus effect - 10 wells with alphanumeric well coding - Positioning notch for automated microscopy - Glass thickness: 0.17 mm Read more: www.gbo.com TABLE OF CONTENTS June 2016 Volume 18, Issue 6 Review News and Views Articles Letters Technical Report Corrigenda Advertisement   HYBRIBODY enables you to get the cDNA clone of your ELISA & IF validated antibody in a couple of months without animal immunization.   Take advantage of our selection & validation service of highly affine single-chain Ab from a humanized synthetic llama VHH library. Watch our video animation to learn more about Hybribody.  Contact our team for more info  Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement ATCC Cell Authentication Service  ATCC offers human cell authentication utilizing STR profiling to meet your funding, publication, and QC/QA/ISO requirements. Identify misidentified or cross-contaminated cells which invalidate research results. Cells are spotted onto barcoded FTA paper in a rapid turnaround process. Test your cell bank, volume pricing is available.   Advertisement Launching in January 2017 - Nature Biomedical Engineering Discovery and technology for human health A new online journal for bench scientists, clinicians and medical engineers. Register for the journal's e-alert to receive content and news from the beginning.   Advertisement Subscribe to Nature with our best current personal subscription offer to access a wealth of scientific articles, features and news every week. Personal subscriptions include app, online, digital and print access so you can enjoy Nature anytime, anywhere.    Review Top The increasing complexity of the ubiquitin code   pp579 - 586 Richard Yau and Michael Rape doi:10.1038/ncb3358 Yau and Rape discuss recent advances in our understanding of the many variations in ubiquitin chain topology and how these mediate ubiquitin-dependent signalling in the cell. News and Views Top Stem cell heterogeneity revealed   pp587 - 589 Marianne S. Andersen and Kim B. Jensen doi:10.1038/ncb3368 The skin forms a protective, water-impermeable barrier consisting of heavily crosslinked epithelial cells. However, the specific role of stem cells in sustaining this barrier remains a contentious issue. A detailed analysis of the interfollicular epidermis now proposes a model for how a composite of cells with different properties are involved in its maintenance. See also: Article by Sada et al. PGC1α drives a metabolic block on prostate cancer progression   pp589 - 590 Martina Wallace and Christian M. Metallo doi:10.1038/ncb3365 Metabolic rewiring is essential for cancer cell survival. PGC1α, a transcriptional co-activator that is downregulated in prostate cancer, is now shown to control prostate cancer metabolism by activating an oxidative metabolic program that prevents tumour growth and metastatic dissemination. See also: Article by Torrano et al. Primary cilia mechanosensing triggers autophagy-regulated cell volume control   pp591 - 592 Zsuzsanna Takacs and Tassula Proikas-Cezanne doi:10.1038/ncb3366 The primary cilium and the process of autophagy are thought to be in a functionally reciprocal relationship. In further support of this link, fluid flow sensing by the primary cilium is now shown to induce autophagy, which in turn regulates the volume of kidney epithelial cells. See also: Article by Orhon et al. Replicating repetitive DNA   pp593 - 594 Silvia Tognetti and Christian Speck doi:10.1038/ncb3367 The function and regulation of repetitive DNA, the 'dark matter' of the genome, is still only rudimentarily understood. Now a study investigating DNA replication of repetitive centromeric chromosome segments has started to expose a fascinating replication program that involves suppression of ATR signalling, in particular during replication stress. See also: Letter by Aze et al. Cell Biology JOBS of the week Postdoctoral positions in stem cell biology and regenerative medicine University of Southern California Postdoctoral Training Fellow in Molecular Proteomics and Molecular Cell Biology Francis Crick Institute Postdoctoral position medical Biochemistry / cell biology / cancer biology University of Gothenburg (GU) Postdoctoral Fellow in Hematopoietic Stem Cell Biology and Blood Disorders Brigham & Women's Hospital - Harvard Medical School Postdoctoral researcher in somatic stem cell biology of the liver and / or pancreas ETH Zurich - The Swiss Federal Institute of Technology in Zurich More Science jobs from Cell Biology EVENT The Brain Mosaic: cellular heterogeneity in the CNS 22.09.16 Leuven, Belgium More science events from Advertisement Nature Index 2016 Saudi Arabia Saudi Arabia had the largest growth of its region in the production of high-quality research in 2015 tracked by the Nature Index, propelling the country into a leading position. Click here to see how strong and fruitful collaborations with international powerhouses have been integral to Saudi Arabia’s rapid rise. The country has now firmly set its sights on becoming a global player in science. Produced with support from: KACST   Articles Top Medial HOXA genes demarcate haematopoietic stem cell fate during human development   pp595 - 606 Diana R. Dou, Vincenzo Calvanese, Maria I. Sierra, Andrew T. Nguyen, Arazin Minasian et al. doi:10.1038/ncb3354 Mikkola and colleagues show that medial HOXA gene expression, induced by retinoic acid signalling, marks the establishment of the definitive HSC fate and controls HSC identity and function. Chronic interleukin-1 exposure drives haematopoietic stem cells towards precocious myeloid differentiation at the expense of self-renewal   pp607 - 618 Eric M. Pietras, Cristina Mirantes-Barbeito, Sarah Fong, Dirk Loeffler, Larisa V. Kovtonyuk et al. doi:10.1038/ncb3346 Passegue and colleagues reveal that pro-inflammatory IL-1 accelerates cell division and induces PU.1-mediated differentiation of HSCs into myeloid cells, whereas chronic IL-1 exposure compromises HSC function. Defining the cellular lineage hierarchy in the interfollicular epidermis of adult skin   pp619 - 631 Aiko Sada, Fadi Jacob, Eva Leung, Sherry Wang, Brian S. White et al. doi:10.1038/ncb3359 Using long-term lineage tracing and mathematical modelling, Tumbar and colleagues define two separate stem cell populations in the epidermis that are regionally clustered, molecularly distinct and show different proliferation dynamics. See also: News and Views by Andersen & Jensen Oncogenic mTOR signalling recruits myeloid-derived suppressor cells to promote tumour initiation   pp632 - 644 Thomas Welte, Ik Sun Kim, Lin Tian, Xia Gao, Hai Wang et al. doi:10.1038/ncb3355 Welte et al. report that mTOR signalling regulates G-CSF production and accumulation of myeloid-derived suppressor cells (MDSCs) to the tumour site, which promotes the tumour-initiating capacity of cancer cells by activating Notch signalling. The metabolic co-regulator PGC1α suppresses prostate cancer metastasis   pp645 - 656 Veronica Torrano, Lorea Valcarcel-Jimenez, Ana Rosa Cortazar, Xiaojing Liu, Jelena Urosevic et al. doi:10.1038/ncb3357 Torrano et al. use bioinformatics analyses to identify PGC1α as a transcriptional regulator of a metabolic program downstream of ERRα that opposes metastatic dissemination in prostate cancer. See also: News and Views by Wallace & Metallo Primary-cilium-dependent autophagy controls epithelial cell volume in response to fluid flow   pp657 - 667 Idil Orhon, Nicolas Dupont, Mohamad Zaidan, Valerie Boitez, Martine Burtin et al. doi:10.1038/ncb3360 Orhon et al. report that primary-cilium-mediated fluid flow sensing triggers autophagy through LKB1-AMPK-mTOR signalling, and thereby controls the volume of kidney epithelial cells. See also: News and Views by Takacs & Proikas-Cezanne Advertisement nature.com webcasts Nature Publishing Group presents a custom webcast on: Overcoming current challenges with 3D cell culturing Wednesday June 15, 2016 8AM PDT, 11AM EDT, 4PM BST, 5PM CEST Register for the webcast and live Q&A session Sponsored by: Lonza Bioscience Solutions Custom webcast: sponsor retains sole responsibility for content     Letters Top Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation   pp668 - 675 Edward H. Hinchcliffe, Charles A. Day, Kul B. Karanjeet, Sela Fadness, Alyssa Langfald et al. doi:10.1038/ncb3348 Chromosome missegregation can lead to p53 activation to block proliferation of aneuploid cells. Hinchcliffe, Dong and colleagues find that generation and spreading of a histone H3.3 Ser31 phosphorylation mark mediates this response. Avoiding artefacts when counting polymerized actin in live cells with LifeAct fused to fluorescent proteins   pp676 - 683 Naomi Courtemanche, Thomas D. Pollard and Qian Chen doi:10.1038/ncb3351 Pollard and colleagues demonstrate in vitro and in fission yeast that the LifeAct actin probe can affect actin filament nucleation and dynamics and perturb actin-dependent cellular processes unless low concentrations are used. Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression   pp684 - 691 Antoine Aze, Vincenzo Sannino, Paolo Soffientini, Angela Bachi and Vincenzo Costanzo doi:10.1038/ncb3344 Costanzo and colleagues find, by reconstitution of repetitive centromeric DNA replication in Xenopus egg extracts, that replication is facilitated by suppression of ATR-mediated checkpoint signalling and formation of DNA loop structures. See also: News and Views by Tognetti & Speck Sister chromatid resolution is an intrinsic part of chromosome organization in prophase   pp692 - 699 Kota Nagasaka, M. Julius Hossain, M. Julia Roberti, Jan Ellenberg and Toru Hirota doi:10.1038/ncb3353 The imaging of individually labelled sister chromatids allows Nagasaka et al. to conclude that mitotic sister chromatin resolution begins in prophase and depends on the activity of topoisomerase II and condensin II, but not on cohesin dissociation. Technical Report Top Self-organization of the human embryo in the absence of maternal tissues   pp700 - 708 Marta N. Shahbazi, Agnieszka Jedrusik, Sanna Vuoristo, Gaelle Recher, Anna Hupalowska et al. doi:10.1038/ncb3347 Zernicka-Goetz and colleagues report an in vitro culture system that recapitulates hallmarks of human embryo morphogenesis before gastrulation, including formation of the pro-amniotic cavity and appearance of the prospective yolk sac. Corrigenda Top Corrigendum: A splicing switch from ketohexokinase-C to ketohexokinase-A drives hepatocellular carcinoma formation   p709 Xinjian Li, Xu Qian, Li-Xia Peng, Yuhui Jiang, David H. Hawke et al. doi:10.1038/ncb3361 Corrigendum: SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture   p709 Manuel Hilbert, Akira Noga, Daniel Frey, Virginie Hamel, Paul Guichard et al. doi:10.1038/ncb3362 Top Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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