Nature Methods Application Notes e-UPDATE: 10 November 2015

If you are unable to see the message below, click here to view.

Search/browse Application Notes

10 November 2015  Application Notes bring you the latest information about innovative tools and technologies and their applications in the lab. We hope that you will find this service useful and informative and encourage you to sign up for future Updates to ensure that you never miss one!

Register to continue receiving application notes updates

FEATURED APPLICATION NOTE Quantitative analysis tools and correlative imaging applications for N-STORM www.nikon.com > Super-resolution techniques have had a significant impact on our understanding of biological processes at the molecular level. However, one of the challenges to their broad utilization has been our limited ability to quantitatively analyze super-resolution images of complex biological tissues. In this Application Note, we highlight recent work by Dudok et al. utilizing Nikon's N-STORM system to develop new correlative imaging methods and quantitative analysis tools to study the mechanism of cannabinoid signaling in the brain. PDF

		DNA library construction using Gibson Assembly® www.syntheticgenomics.com > Since its introduction 6 years ago, the Gibson Assembly method has been widely adopted as a preferred cloning method. Here we describe an application of Gibson Assembly beyond routine cloning: assembly in DNA library construction. Advantages of using Gibson Assembly, specifically the SGI-DNA Gibson Assembly HiFi 1 Step kit, in library construction include speed, efficiency, scarless assembly with vector and versatility. PDF

		The Role of Multiplexed Assays in Vaccine Development www.luminexcorp.com > Vaccine development is a complex and lengthy process, involving multiple stages. The goal of the first stage is to understand the disease burden and pathogenicity of a disease, including all of the variants of the disease-causing organism, and identify the antigens. Next, the preclinical stage of development begins with antigen characterization and purification, in order to develop vaccine candidates. PDF

		NGS library preparation for balanced, comprehensive methylome coverage from low-input quantities www.swiftbiosci.com > Next-generation sequencing (NGS) of bisulfite-converted DNA to detect methylation status with per-base resolution is currently restricted by the requirement of large amounts of input material. Here we show how the Accel-NGS® Methyl-Seq DNA library kit, with its unique chemistry, enables the construction of high-complexity libraries from 1 ng of input. With this kit, scientists will no longer be bforced to sacrifice library quality when using low quantities of starting material. PDF

		Automated live cell imaging of cell migration across a microfluidic-controlled chemoattractant gradient www.millipore.com > The quantification of cell-migration behavior in response to gradients is critical for understanding cellular movement and development. The CellASIC® ONIX microfluidic platform offers a microfluidic gradient plate to enable precision-controlled chemoattractant diffusion across perfusion barriers, enabling a quantitatively defined diffusion gradient stable enough for long-term, live cell analysis. Using the system, we studied the effect of a serum gradient on metastatic breast cancer cell migration distance, velocity and degree of chemotaxis. PDF

		MAGNETOFECTION™,the tool of choice to reach high transfection andtransduction efficiency with low toxicity on Primary cells www.ozbiosciences.com > Magnetofection is a powerful method for introducing nucleic acids into cells, that successful overcomes the limitations encountered by traditional nucleic acid delivery systems such as low efficiency, toxicity, difficulty to handle. Magnetofection™ has been successfully tested on a broad range of cell lines, hard-to-transfect and primary cells. It is perfect for non-dividing or slowly dividing cells, meaning that the genetic materials can go to the nucleus without cell division. PDF

		Optimized lysis system: ideal for extraction of genomic DNA from different kind of FFPE tissue samples www.analytik-jena.com > Analytik Jena's blackPREP FFPE DNA Kit is optimized for DNA extraction from a variety of FFPE (formalin-fixed, paraffin-embedded) tissue sample, e.g. adipose tissue, pancreatic tissue or placenta as starting materials. PDF

 

Application Notes are supplied by commercial companies. The Nature Publishing Group does not endorse any of the products or services advertised here. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department For print subscription enquiries, please contact our subscription department For other enquiries, please contact our feedback department Nature Publishing Group | One New York Plaza, Suite 4500 | New York | NY 10004-1562 | USA Nature Publishing Group's worldwide offices: London - Paris - Munich - New Delhi - Tokyo - Melbourne San Diego - San Francisco - Washington - New York - Boston