Nature Cell Biology contents: November 2015 Volume 17 Number 11, pp 1371 - 15120

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Advertisement New! 3D cell culture for in vivo epigenetic experiments Diagenode offers a revolutionary solution to enhance your epigenetics research using 3D micro pattern cell culture plates Physiologically and morphologically relevant: Adherent cells form natural spheroids to measure of cell-to-cell interactions, not cell-to plastic Easy and ready-to-use: No specific expertise required--just use right out of the box! Diverse applications: ChIP-seq, drug/inhibitor screening, toxicity assays, cell differentiation, and more Learn more TABLE OF CONTENTS November 2015 Volume 17, Issue 11 News and Views Articles Letters Corrigendum Advertisement Live cell imaging? Kill the antibodies and Save your Cells! Intracellular epitopes typically require fixation and permeabilization. Find out how you can detect targets of interest without destroying your cells.  Learn about SmartFlare™ live cell biomarker detection technology today! Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement Nature Plants: Call for Papers Nature Plants launched in January and covers all aspects of plants be it their evolution, genetics, development or metabolism, their interactions with the environment, or their societal significance. The journal welcomes high quality submissions and encourages you and your colleagues to consider submitting your next research paper to the journal.  Submit your next research paper to the journal.     Advertisement Nature Reviews Drug Discovery Special on targeting the IL-17 pathway in inflammatory disease POSTER - Targeting the IL-17 pathway in inflammatory disease   ANIMATION - Tumour immunology and immunotherapy Animation and Poster produced with support from  Eli Lilly and Company   News and Views Top A mammalian pexophagy target   pp1371 - 1373 Suresh Subramani doi:10.1038/ncb3253 Protein ubiquitylation in mammals is known to trigger selective autophagy of peroxisomes through a process termed pexophagy. The physiological peroxisomal target for pexophagy-related ubiquitylation has been controversial, but two studies have now identified the protein PEX5 as the real candidate. Endosomal integrin signals for survival   pp1373 - 1375 Elena Rainero and Jim C. Norman doi:10.1038/ncb3261 The mechanisms underlying integrin-dependent signalling are a topic of continued study. Endocytosed integrins are now shown to drive assembly of signalling complexes on the cytoplasmic face of endocytic membranes to promote cancer cell survival and increase metastatic capacity following cell detachment. See also: Article by Alanko et al. RNF138 joins the HR team   pp1375 - 1377 Simon Bekker-Jensen and Niels Mailand doi:10.1038/ncb3262 Two studies show that the E3 ubiquitin ligase RNF138 is recruited to DNA double-strand break sites, where it ubiquitylates key repair factors to promote DNA-end resection and homologous recombination. These findings add insights into the multilayered regulatory mechanisms underlying DNA double-strand break repair pathway choice in mammalian cells. See also: Article by Ismail et al. | Article by Schmidt et al. Cell Biology JOBS of the week Research Group Leader – Cell Biology Medical Research Council Assistant Professor of Cell Biology and Neuroscience Rutgers University Immunology and Cell Biology Beth Israel Deaconess Med Ctr / Harvard Medical School Tenured / Tenure-Track Faculty Positions in Stem Cell Biology University of Notre Dame Post-doctoral Position Stem Cell Biology Odense University Hospital More Science jobs from Cell Biology EVENT Islet Biology: from Cell Birth to Death 13.03.16 Keystone, USA More science events from Advertisement Unravel the hidden mysteries of histones, nucleosomes, and chromatin!  New additions to Diagenode's epigenetics portfolio: Chromatin Assembly Kit – assemble a chromatin model in less than 6 hours Native recombinant nucleosomes – ultra-pure and ideal for structural changes from histones variants studies Recombinant human histones -- ideal for histone post-translational modifications studies Learn more and uncover chromatin mysteries   Articles Top Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming   pp1379 - 1387 Tianhua Ma, Jun Li, Yue Xu, Chen Yu, Tao Xu et al. doi:10.1038/ncb3256 Ding and colleagues show that somatic cell reprogramming does not depend on Atg5-dependent canonical autophagy, but requires mitochondrial clearance in an Atg5-independent manner downstream of AMPK. Microtubule-driven nuclear rotations promote meiotic chromosome dynamics   pp1388 - 1400 Nicolas Christophorou, Thomas Rubin, Isabelle Bonnet, Tristan Piolot, Marion Arnaud et al. doi:10.1038/ncb3249 Huynh and colleagues discover nuclear rotations driven by centrosomes, microtubules and Dynein in Drosophila germ cells, and find that these movements facilitate the pairing of homologous chromosomes. Heterochromatic breaks move to the nuclear periphery to continue recombinational repair   pp1401 - 1411 Taehyun Ryu, Brett Spatola, Laetitia Delabaere, Katherine Bowlin, Hannah Hopp et al. doi:10.1038/ncb3258 Chiolo and colleagues find that, in a SUMOylation-dependent manner, heterochromatic double-strand breaks move to the nuclear periphery where Rad51 is recruited to continue repair. Integrin endosomal signalling suppresses anoikis   pp1412 - 1421 Jonna Alanko, Anja Mai, Guillaume Jacquemet, Kristine Schauer, Riina Kaukonen et al. doi:10.1038/ncb3250 Ivaska and colleagues report that endocytosed integrins are able to signal from endosomes in an FAK-dependent manner. They further show that endosomal integrin signalling can promote anoikis resistance and lung colonization in cancer cells. See also: News and Views by Rainero & Norman Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module   pp1422 - 1434 Johanna Roostalu, Nicholas I. Cade and Thomas Surrey doi:10.1038/ncb3241 Using TIRF-based in vitro reconstitution assays Surrey and colleagues characterize how chTOG and TPX2 cooperate in microtubule nucleation and find that importins regulate the process. Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization   pp1435 - 1445 Alexis J. Lomakin, Kun-Chun Lee, Sangyoon J. Han, Duyen A. Bui, Michael Davidson et al. doi:10.1038/ncb3246 Lomakin et al. report that the competition for actin between two distinct F-actin networks determines whether epithelial cells remain stationary or migrate, with myosin II inhibiting the migratory polarized phenotype by confining actin in contractile bundles. The RNF138 E3 ligase displaces Ku to promote DNA end resection and regulate DNA repair pathway choice   pp1446 - 1457 Ismail Hassan Ismail, Jean-Philippe Gagné, Marie-Michelle Genois, Hilmar Strickfaden, Darin McDonald et al. doi:10.1038/ncb3259 Jackson and colleagues and Hendzel and colleagues reveal that the E3 ligase RNF138 functions in the repair of double-strand breaks by promoting CtIP accumulation and displacement of DNA-PK subunit Ku. See also: News and Views by Bekker-Jensen & Mailand Systematic E2 screening reveals a UBE2D–RNF138–CtIP axis promoting DNA repair   pp1458 - 1470 Christine K. Schmidt, Yaron Galanty, Matylda Sczaniecka-Clift, Julia Coates, Satpal Jhujh et al. doi:10.1038/ncb3260 Jackson and colleagues and Hendzel and colleagues reveal that the E3 ligase RNF138 functions in the repair of double-strand breaks by promoting CtIP accumulation and displacement of DNA-PK subunit Ku. See also: News and Views by Bekker-Jensen & Mailand Activator–inhibitor coupling between Rho signalling and actin assembly makes the cell cortex an excitable medium   pp1471 - 1483 William M. Bement, Marcin Leda, Alison M. Moe, Angela M. Kita, Matthew E. Larson et al. doi:10.1038/ncb3251 Using live imaging of Xenopus and starfish oocytes and embryos undergoing cytokinesis, Bement and colleagues show that anaphase onset promotes cortical waves of Rho and F-actin, which can be modelled by reaction–diffusion dynamics. 6-Phosphogluconate dehydrogenase links oxidative PPP, lipogenesis and tumour growth by inhibiting LKB1–AMPK signalling   pp1484 - 1496 Ruiting Lin, Shannon Elf, Changliang Shan, Hee-Bum Kang, Quanjiang Ji et al. doi:10.1038/ncb3255 Chen and colleagues report that the third enzyme in the oxidative pentose phosphate pathway (PPP), 6PGD, controls cancer cell proliferation by regulating LKB1–AMPK signalling. Inhibitors of 6PGD decrease tumorigenesis in mouse xenografts. Advertisement Save days by automating multistep purifications. Now that's a bright idea.   Having to think twice before automating tandem or multidimensional (Multi-D) protocols is now a thing of the past - preprogrammed method templates from the NGC™ Chromatography System's new Multi-D template library make automation so simple that anyone can automate their protocol. See how easy it is to convert your methods to Multi-D. Watch the video.   Letters Top Lateral adhesion drives reintegration of misplaced cells into epithelial monolayers   pp1497 - 1503 Dan T. Bergstralh, Holly E. Lovegrove and Daniel St Johnston doi:10.1038/ncb3248 Using live imaging, St Johnston and colleagues show in three Drosophila epithelia that cells born outside the epithelium do not die, but reintegrate, and that lateral adhesion is required for reintegration to occur. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP   pp1504 - 1511 Adam B. Johnston, Agnieszka Collins and Bruce L. Goode doi:10.1038/ncb3252 Goode and colleagues report that Twinfilin and Srv2 cooperate to accelerate depolymerization of actin filaments. Corrigendum Top Corrigendum: Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module   p1512 Johanna Roostalu, Nicholas I. Cade and Thomas Surrey doi:10.1038/ncb3265 Top Advertisement An open access journal dedicated to highlighting the most important scientific advances in Parkinson's disease research, spanning the motor and non-motor disorders of Parkinson's disease. Part of the Nature Partner Journals series, npj Parkinson's Disease is published in partnership with the Parkinson's Disease Foundation. 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