Nature Cell Biology contents: February 2013 Volume 15 Number 2, pp 125 - 229

Advertisement Save on best-in-class personal flow cytometry  With a combined value package, you can save 10% on the BD Accuri™ C6 personal flow cytometer and 40% on high quality BD Pharmingen™ reagents for 2 full years. There's never been a better time to buy and get the best-in-class power, flexibility, and insight personal flow cytometry can offer. Flow cytometry within reach™. bdbiosciences.com/go/accuri TABLE OF CONTENTS February 2013 Volume 15, Issue 2 Turning Points Review News and Views Research Highlights Articles Letters Erratum Corrigendum Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement The NYSCF – Robertson Innovator Awards for Early Career Investigators in Neuroscience The New York Stem Cell Foundation (NYSCF) supports bold, innovative science with the potential to transform the field of neuroscience through grants to early career scientists. NYSCF requests applications for Innovator Awards for early career investigators in neuroscience in the fundamental areas of developmental, cellular, cognitive and behavioral neuroscience, broadly interpreted. Proposals do not need to be related to stem cells. The awards provide up to $1.5 million USD over 5 years. Application details available online at www.nyscf.org/neuro. Due: March 22, 2013   Advertisement Fluorescent proteins and sensors: A practical discussion Fluorescent proteins have become invaluable tools for fluorescent microscopy in the life sciences but researchers still have many practical questions about using them. Three experts in the development and use of fluorescent proteins and sensors discuss these challenges and provide practical advice to users. Register for FREE   Turning Points Top Virus entry: What has pH got to do with it?   p125 Ari Helenius doi:10.1038/ncb2678 Review Top Tracing the cellular origin of cancer   pp126 - 134 Cédric Blanpain doi:10.1038/ncb2657 Cédric Blanpain discusses the progress achieved in identifying and characterizing the cellular origins of different solid tumours in mouse models of skin, brain, breast, gut and lung cancer, using genetic lineage tracing approaches. News and Views Top Rab10 joins the ER social network   pp135 - 136 Jaerak Chang and Craig Blackstone doi:10.1038/ncb2682 The endoplasmic reticulum (ER) is a heterogeneous organelle with distinct morphologies of sheets and an interconnected network of tubules sharing a common lumen. An ER domain marked by the Rab10 GTPase and several lipid-synthesizing enzymes is implicated in dynamic ER tubule formation and fusion events in cells. See also: Article by English & Voeltz Osmotic regulation of seamless tube growth   pp137 - 139 Jodi Schottenfeld-Roames and Amin S. Ghabrial doi:10.1038/ncb2683 Most organs are composed of tubes of differing cellular architectures, including intracellular 'seamless' tubes. Two studies examining the morphogenesis of the seamless tubes formed by the excretory canal cell in Caenorhabditis elegans reveal a previously unappreciated role for osmoregulation of tubulogenesis: hyperosmotic shock recruits canalicular vesicles to the lumenal membrane to promote seamless tube growth. See also: Article by Khan et al. | Article by Kolotuev et al. miR-29b moulds the tumour microenvironment to repress metastasis   pp139 - 140 Sonia A. Melo and Raghu Kalluri doi:10.1038/ncb2684 Tumour cells are influenced by their microenvironment, which can promote uncontrolled growth, invasion and metastasis. The GATA3 transcription factor is now shown to regulate the tumour microenvironment by inducing the expression of miR-29b in cancer cells. This microRNA in turn inhibits the expression of genes involved in angiogenesis and extracellular matrix signalling and remodelling to suppress metastasis. See also: Article by Chou et al. Cell Biology JOBS of the week Postdoctoral Fellow University of Wisconsin - Madison Professor / Reader University of Bristol International PhD Program Molecular Neurobiology Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch - Helmholtz Association Postdoctoral researcher Columbia University Medical Center BioImage Informatics Team Leader Scionics Computer Innovation GmbH More Science jobs from Cell Biology EVENT Computational Cell Biology: The Interplay Between Models and Experimentation 19-22 March 2013 Cold Spring Harbor, US More science events from Research Highlights Top Focal adhesions tug at matrix for rigidity sensing | Sorting Vangl2 at the Golgi | Scaling somitogenesis | Interrogating Lkb1 function Advertisement Small Reactor - Big Results With CELLSTAR® CELLreactor™ • Innovative: 50 ml filter tube for suspension cultures • Safe: USP VI-certified 0.2 µm PTFE-coated capillary • membrane • Fast: Facilitates parallel experiments • Simple: Cultivation and harvest in one tube • Easy: Mixing by standard equipment More: www.gbo.com/bioscience   Articles Top Intracellular lumen extension requires ERM-1-dependent apical membrane expansion and AQP-8-mediated flux   pp143 - 156 Liakot A. Khan, Hongjie Zhang, Nessy Abraham, Lei Sun, John T. Fleming, Matthew Buechner, David H. Hall and Verena Gobel doi:10.1038/ncb2656 Gobel and colleagues show that the cortical protein ERM-1 drives expansion of the unicellular tube that constitutes the Caenorhabditis elegans excretory canal by recruiting membrane and cytoskeletal components, and the water channel aquaporin, to the apical side of the tube. See also: News and Views by Schottenfeld-Roames & Ghabrial A pathway for unicellular tube extension depending on the lymphatic vessel determinant Prox1 and on osmoregulation   pp157 - 168 Irina Kolotuev, Vincent Hyenne, Yannick Schwab, David Rodriguez and Michel Labouesse doi:10.1038/ncb2662 Labouesse and colleagues examine the steps of excretory canal growth in nematodes. They delineate the importance of osmoregulated vesicle fusion with the lumen, and of a subapical cytoskeletal web to ensure straight lumen growth. They identify PROS-1 as a transcription factor essential for lumen growth through modulation of the osmosensitive kinase GCK-3 and intermediate filament protein IFB-1. See also: News and Views by Schottenfeld-Roames & Ghabrial Rab10 GTPase regulates ER dynamics and morphology   pp169 - 178 Amber R. English and Gia K. Voeltz doi:10.1038/ncb2647 ER tubules grow and fuse to give the ER its characteristic shape. English and Voeltz show that the small GTPase Rab10 is crucial for ER tubule growth and fusion. Rab10 localizes to the leading edge of new ER tubules with enzymes that promote phospholipid synthesis, suggesting that ER tubule growth, fusion and phospholipid synthesis might be coupled. See also: News and Views by Chang & Blackstone SCFFbxw5 mediates transient degradation of actin remodeller Eps8 to allow proper mitotic progression   pp179 - 188 Achim Werner, Andrea Disanza, Nina Reifenberger, Gregor Habeck, Janina Becker, Matthew Calabrese, Henning Urlaub, Holger Lorenz, Brenda Schulman, Giorgio Scita and Frauke Melchior doi:10.1038/ncb2661 Cortical actin is implicated in cell shape regulation during mitosis. Melchior and colleagues reveal that SCFFbxw5-mediated ubiquitylation and degradation of the actin remodeller Eps8 is required for timely cell rounding and progression into metaphase, whereas the capping activity of Eps8 is needed for mitotic exit. Loss of Par3 promotes breast cancer metastasis by compromising cell–cell cohesion   pp189 - 200 Bin Xue, Kannan Krishnamurthy, D. Craig Allred and Senthil K. Muthuswamy doi:10.1038/ncb2663 Muthuswamy and colleagues demonstrated that loss of the Par3 polarity protein cooperates with ErbB2 activity to promote cell invasion and metastasis by destabilizing E-cadherin-dependent cell–cell junctions. GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression   pp201 - 213 Jonathan Chou, Jeffrey H. Lin, Audrey Brenot, Jung-whan Kim, Sylvain Provot and Zena Werb doi:10.1038/ncb2672 Werb and colleagues demonstrate that GATA3, a transcription factor that promotes luminal differentiation in the mammary gland, suppresses breast cancer metastasis to the lung by upregulating miR-29b. This microRNA suppresses pro-metastatic characteristics, including mesenchymal traits and the expression of microenvironmental factors involved in angiogenesis and extracellular matrix remodelling. See also: News and Views by Melo & Kalluri Advertisement The 3rd Stem Cells for Drug Developers meeting (19 - 21 March 2013, Boston) will bring leading drug developers and world class academics to discuss and debate the scientific issues to drive forward the commercialization of stem cell-derived models. Overcome the scientific hurdles to harness the full potential of stem cells in your R&D activities.   Letters Top Direct lineage reprogramming of post-mitotic callosal neurons into corticofugal neurons in vivo    pp214 - 221 Caroline Rouaux and Paola Arlotta doi:10.1038/ncb2660 Arlotta and Rouaux show that expression of the transcription factor Fezf2 in vivo is sufficient to redirect post-mitotic callosal projection neurons from one particular layer of the brain to corticofugal projection neurons that pertain to a different layer, including a redirection of their axonal connectivity. Amputation-induced reactive oxygen species are required for successful Xenopus tadpole tail regeneration   pp222 - 228 Nick R. Love, Yaoyao Chen, Shoko Ishibashi, Paraskevi Kritsiligkou, Robert Lea, Yvette Koh, Jennifer L. Gallop, Karel Dorey and Enrique Amaya doi:10.1038/ncb2659 Xenopus laevis and tropicalis tadpoles display incredible regenerative capacity of their tail. Amaya and colleagues find that tadpole tail amputation induces the production of reactive oxygen species (ROS) to induce cell proliferation and regeneration, through activation of the Wnt/β-catenin and Fgf20 signalling pathways. Erratum Top Phosphorylation-enabled binding of SGO1-PP2A to cohesin protects sororin and centromeric cohesion during mitosis   p229 Hong Liu, Susannah Rankin and Hongtao Yu doi:10.1038/ncb2688 Corrigendum Top Humans put their eggs in more than one basket   p229 Diana J. Laird doi:10.1038/ncb2694 Top Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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