Nature Cell Biology contents: December 2011 Volume 13 Number 12, pp 1385 - 1467

Advertisement See the Light. Make the Switch. Stop Cloning! The LightSwitch Luciferase Assay System provides everything you need for reporter assays: pre-cloned human promoters and 3''UTRs in luciferase reporter vectors plus optimized luciferase reagents to measure promoter induction and miRNA target validation. Visit SwitchGear Genomics and explore our genome-wide collections and validated pathway sets. TABLE OF CONTENTS December 2011 Volume 13, Issue 12 Comment Turning Points News and Views Research Highlights Articles Letters Resource Corrigenda Addendum Recommend to your library Web feed Available online only Subscribe Advertisement Duolink-"In-cell Co-IP" Find out why Duolink is a better alternative for your Co-IP assay • Detect weak and transient interactions • Faster, more reliable, higher throughput • Achieve localization - see what's really there • Over 200 publications and growing fast.... More information and special webshop offer www.olink.com/ Nature Cell Biology is on Twitter Advertisement ZEISS Efficient Navigation: ZEN 2011 Now available for all wide field microscopy systems Setting up your complex experiments can be a challenging task. With the Smart Setup feature, you select your fluorophores and imaging requirements and the system is automatically configured for you. You can easily optimize for such features as fast acquisition, live cell imaging or high resolution. Navigation in the world of microscopy has never been easier. Learn more on the ZEN website: www.zeiss.com/zen Download ZEN-Lite free of charge: www.zeiss.com/zen-lite   Advertisement Is your washer limiting your results? Benefit from Tecan's new HydroSpeed™ expert plate washer, offering: • extra gentle Cell Protection™ • wash settings • dual magnets for efficient • magnetic bead processing • Anti-Clogging™ function for • reliable ELISA washing  Learn more   Comment Top Cell biology in India: The future needs an international perspective p1385 Satyajit Mayor doi:10.1038/ncb2391 There is a lack of trained scientists to fill the increasing number of jobs and funding opportunities in the Indian scientific research sector. This is a great opportunity for the international scientific community to help build and nurture a vibrant cell biology research community in India. Full Text | PDF Turning Points Top Heeding a mentor's advice: A lesson in persistence p1386 Mina J. Bissell doi:10.1038/ncb2392 Full Text | PDF News and Views Top Phosphatidylserine promotes polar Cdc42 localization pp1387 - 1388 Tina Freisinger and Roland Wedlich-Soldner doi:10.1038/ncb2382 The establishment and maintenance of cell polarity requires targeted recruitment of polarity regulators to the plasma membrane. Phosphatidylserine is now shown to have a key role in polarization of yeast cells and the localization of the central polarity regulator Cdc42. Full Text | PDF See also: Letter by Fairn et al. A new cap for kinetochore fibre minus ends pp1389 - 1391 Sabine Petry and Ronald D. Vale doi:10.1038/ncb2387 In mitotic spindles, each sister chromatid is directly attached to a spindle pole through microtubule bundles known as kinetochore fibres. Microspherule protein 1 (MCRS1) is now shown to support spindle assembly by localizing to the minus ends of kinetochore fibres and protecting them from depolymerization. Full Text | PDF See also: Article by Meunier & Vernos Pulling together and pulling apart: collective cargo movement in eukaryotic cells pp1391 - 1392 Dyche Mullins doi:10.1038/ncb2393 To establish and maintain their internal organization, living cells must move molecules to their correct locations. Long-range intracellular movements are often driven by motor molecules moving along microtubules, similarly to trucks driving along a highway. Recent work demonstrates that some randomly dispersed cargos can generate actin filaments that form a connected network whose contraction drives collective cargo movement. Full Text | PDF See also: Letter by Schuh Cell Biology JOBS of the week Graduate Student Positions available in Microfluidics & Bioanalysis University of Texas at El Paso Research Fellow University of Birmingham Postdoctoral position - Geographer / GIS specialist / Computer scientist / Biologist University of Helsinki / BCI More Science jobs from Cell Biology EVENT High-Throughput Structural Biology (J5) / Structural Biology of Cellular Processes: From Atoms to Cells (J6) 22.-27.01.12 CO, US More science events from Research Highlights Top Yap makes the heart grow | Mapping the mitochondria | Hair follicles run by clockwork | Autophagy clears dad's mitochondria Advertisement Webinar on Demand: Technical Tips for qPCR Assay Design and Setup This webinar focuses on 5' nuclease assay design and experimental setup. Guidelines for using design parameters such as Tm, GC content, amplicon size, and location of primers and probe are provided. Several free, online tools that can aid in these processes are also discussed.   Articles Top The opposing transcriptional functions of Sin3a and c-Myc are required to maintain tissue homeostasis pp1395 - 1405 Elisabete M. Nascimento, Claire L. Cox, Stewart MacArthur, Shobbir Hussain, Matthew Trotter, Sandra Blanco, Menon Suraj, Jennifer Nichols, Bernd Kubler, Salvador Aznar Benitah, Brian Hendrich, Duncan T. Odom and Michaela Frye doi:10.1038/ncb2385 The transcriptional role of c-Myc in maintaining tissue homeostasis is still unclear. Using mice conditionally expressing an activated form of c-Myc in the epidermis, and genome-wide approaches, Frye and colleagues show that c-Myc modulates the expression of the epidermal differentiation complex locus in the skin by displacing or recruiting specific transcriptional regulators. c-Myc activity is negatively regulated in vivo in this context by Sin3a. Abstract | Full Text | PDF K-fibre minus ends are stabilized by a RanGTP-dependent mechanism essential for functional spindle assembly pp1406 - 1414 Sylvain Meunier and Isabelle Vernos doi:10.1038/ncb2372 Chromosomal microtubules participate in formation of kinetochore fibres by attaching their plus ends at kinetochores and focusing their minus ends at the spindle poles. Vernos and colleagues show that the centrosome-localized protein MCRS1 accumulates to chromosomal microtubule minus ends in a RanGTP-dependent manner to prevent microtubule depolymerization and to promote kinetochore-fibre stability and spindle assembly. Abstract | Full Text | PDF See also: News and Views by Petry & Vale ZNRF1 promotes Wallerian degeneration by degrading AKT to induce GSK3B-dependent CRMP2 phosphorylation pp1415 - 1423 Shuji Wakatsuki, Fuminori Saitoh and Toshiyuki Araki doi:10.1038/ncb2373 Wallerian degeneration occurs in axons following cutting or crush injuries; however, the molecular mechanisms that regulate this process remain elusive. Araki and colleagues find that the ubiquitin ligase ZNRF1 promotes Wallerian degeneration by ubiquitylating AKT, which leads to increased GSK3B activity and subsequent inhibition of the tubulin-binding protein CRMP2. Abstract | Full Text | PDF Advertisement Introducing a new dye: BD Horizon™ PE-CF594 The new BD Horizon PE-CF594 reagent maximizes choice and flexibility by providing an additional bright dye that can be used in multicolor panels. Get more choice, more color, and improved brightness over existing dyes offered for this detector. See sample data and take advantage of free online tools here. bdbiosciences.com/go/bright   Letters Top Phosphatidylserine is polarized and required for proper Cdc42 localization and for development of cell polarity pp1424 - 1430 Gregory D. Fairn, Martin Hermansson, Pentti Somerharju and Sergio Grinstein doi:10.1038/ncb2351 In yeast, polarized localization of Cdc42 is essential for budding and mating, but how polarity is attained has been elusive. Grinstein and colleagues show that phosphatidylserine accumulates in a polar fashion in yeast, and is required for the proper localization of Cdc42. First paragraph | Full Text | PDF See also: News and Views by Freisinger & Wedlich-Soldner An actin-dependent mechanism for long-range vesicle transport pp1431 - 1436 Melina Schuh doi:10.1038/ncb2353 In mammalian cells, long-range vesicular transport is thought to occur via microtubule tracks. However, Schuh reports the existence of an actin-based pathway for long-range trafficking in mouse oocytes by showing that Rab11a-positive vesicles are decorated with actin-nucleating formin proteins. She finds that these proteins assemble actin networks that guide vesicles to the cell surface. First paragraph | Full Text | PDF See also: News and Views by Mullins Caspase 8 inhibits programmed necrosis by processing CYLD pp1437 - 1442 Marie Anne O/'Donnell, Eva Perez-Jimenez, Andrew Oberst, Aylwin Ng, Ramin Massoumi, Ramnik Xavier, Douglas R. Green and Adrian T. Ting doi:10.1038/ncb2362 Caspase 8 is known to suppress necroptosis, but its relevant target protein was unknown. Ting and colleagues show that caspase 8 cleaves the deubiquitylase CYLD to inhibit necroptosis and promote cell survival. First paragraph | Full Text | PDF E-box-independent regulation of transcription and differentiation by MYC pp1443 - 1449 Iris Uribesalgo, Marcus Buschbeck, Arantxa Gutierrez, Sophia Teichmann, Santiago Demajo, Bernd Kuebler, Josep F. Nomdedeu, Juan Martin-Caballero, Guglielmo Roma, Salvador Aznar Benitah and Luciano Di Croce doi:10.1038/ncb2355 The MYC proto-oncogene modulates transcription through binding to E-boxes. Di Croce and colleagues find that PAK-2-mediated phosphorylation confers a tumour-suppressive function to MYC, in which MYC cooperates with differentiation signals to positively modulate the transcription of genes targeted by retinoic acid, independently of E-boxes. First paragraph | Full Text | PDF Cdc14 phosphatase promotes segregation of telomeres through repression of RNA polymerase II transcription pp1450 - 1456 Andres Clemente-Blanco, Nicholas Sen, Maria Mayan-Santos, Maria P. Sacristan, Bryony Graham, Adam Jarmuz, Adam Giess, Elizabeth Webb, Laurence Game, Dirk Eick, Avelino Bueno, Matthias Merkenschlager and Luis Aragon doi:10.1038/ncb2365 Aragon and colleagues show that the phosphatase Cdc14 acts on the CDT subunit of RNA polymerase II to silence transcription of repetitive regions of the yeast genome. At telomeres this event promotes condensin loading and mitotic segregation. First paragraph | Full Text | PDF Resource Top Fibroblast polarization is a matrix-rigidity-dependent process controlled by focal adhesion mechanosensing pp1457 - 1465 Masha Prager-Khoutorsky, Alexandra Lichtenstein, Ramaswamy Krishnan, Kavitha Rajendran, Avi Mayo, Zvi Kam, Benjamin Geiger and Alexander D. Bershadsky doi:10.1038/ncb2370 Bershadsky and colleagues show that fibroblast polarization depends on matrix rigidity and focal adhesion mechanosensing. They target protein tyrosine kinases through RNAi to identify signalling molecules that regulate traction force generation, focal adhesion assembly and mechanosensitivity. Abstract | Full Text | PDF Corrigenda Top p53 regulates epithelial-mesenchymal transition and stem cell properties through modulating miRNAs p1466 Chun-Ju Chang, Chi-Hong Chao, Weiya Xia, Jer-Yen Yang, Yan Xiong, Chia-Wei Li, Wen-Hsuan Yu, Sumaiyah K. Rehman, Jennifer L. Hsu, Heng-Huan Lee, Mo Liu, Chun-Te Chen, Dihua Yu and Mien-Chie Hung doi:10.1038/ncb2401 Full Text | PDF Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity p1467 Tse-Chun Kuo, Chun-Ting Chen, Desiree Baron, Tamer T. Onder, Sabine Loewer, Sandra Almeida, Cara M. Weismann, Ping Xu, Jean-Marie Houghton, Fen-Biao Gao, George Q. Daley and Stephen Doxsey doi:10.1038/ncb2405 Full Text | PDF Addendum Top p53 regulates epithelial-mesenchymal transition and stem cell properties through modulating miRNAs p1467 Chun-Ju Chang, Chi-Hong Chao, Weiya Xia, Jer-Yen Yang, Yan Xiong, Chia-Wei Li, Wen-Hsuan Yu, Sumaiyah K. Rehman, Jennifer L. Hsu, Heng-Huan Lee, Mo Liu, Chun-Te Chen, Dihua Yu and Mien-Chie Hung doi:10.1038/ncb2402 Full Text | PDF Advertisement Visit Nature Publishing Group at booth 300 during the ASCB annual meeting for prizes, discounts and more! 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