 | FEATURED APPLICATION NOTE |  | | Increasing data collection and fidelity by maximizing confocal field of view
https://www.nikon.com/ >
For years, the field of view (FOV) of confocal systems has been limited by the FOV of the microscope they are attached to. With the release of the Nikon Ti2 inverted microscope, the world's first 25-mm FOV became available. Now, Nikon has taken advantage of this improvement by building the largest FOV point scanner in the world, the A1 HD25®. This Application Note focuses on the impact of this technology on simple, everyday experiments. | |
 | | Airyscan detection in multiphoton microscopy: super-resolution and improved signal-to-noise ratio beyond the confocal depth limit
http://www.zeiss.com/microscopy >
The penetration depth of traditional confocal laser-scanning microscopy (LSM) systems is limited by light scattering. To avoid these limitations, multiphoton LSM uses a nonlinear fluorophore excitation process in combination with a non-descanned detection concept to greatly increase the penetration depth. However, in traditional multiphoton LSM, this increased depth necessitates a compromise on the achievable spatial resolution and signal-to-noise compared with that of confocal LSM. The novel Airsycan detection concept from ZEISS, used in combination with multiphoton excitation, overcomes these limitations and provides increased resolution and signal-to-noise with a 2–3× increase in penetration depth compared with that of traditional confocal LSM. | | | |  | | Making difficult things easier and simpler with a manipulation system
http://www.nsk.com/ >
Establishment of physiologically-relevant in vitro models is crucial to further understanding of the mechanisms of neurological diseases as well as targeted drug development. While iPSC-derived neurons show great promise for compound screening and disease modeling, use of three-dimensional (3D) cultures is emerging as a valid approach for neuronal cell based assay development. 3D cultures are recognized as more closely recapitulating aspects of the human tissues including the architecture, cell organization, as well as cell-cell and cell-matrix interactions1,2. | | | |  | | High-content assay for morphological characterization of 3D neuronal networks in a microfluidic platform
https://www.moleculardevices.com/ >
Establishment of physiologically-relevant in vitro models is crucial to further understanding of the mechanisms of neurological diseases as well as targeted drug development. While iPSC-derived neurons show great promise for compound screening and disease modeling, use of three-dimensional (3D) cultures is emerging as a valid approach for neuronal cell based assay development. 3D cultures are recognized as more closely recapitulating aspects of the human tissues including the architecture, cell organization, as well as cell-cell and cell-matrix interactions1,2. | | | |  | | Reducing sample preparation time from Sf9 insect cultures by using Sartoclear Dynamics® Lab
https://www.sartorius.com/sartoclear-dynamics-lab >
In this study, I tested the Sartoclear Dynamics® Lab V500 Kit as a novel method for the clarification of cell culture media prior to purification of a recombinant protein expressed in Sf9 cells. The vector from which this protein is expressed encodes a signal sequence to ensure secretion of the protein from the cells following expression. It is therefore necessary to remove cells from the media before purifying the protein but using traditional methods to do this (centrifugation followed by filtration) can be time consuming. Here, I show that the Sartoclear Dynamics® Lab filter offers significant time savings over conventional methods with no noticeable effect on protein yield, making this product an ideal addition to any laboratory looking to increase productivity and throughput from their insect cell expression systems. | | | |  | | A study on the efficacy of Prime&Bond active, the new active universal adhesive from Dentsply Sirona
https://corporate.dentsplysirona.com/en.html >
Prime&Bond active™ is a one-component universal dental adhesive which brings new advantages to the universal adhesive arena. For eff ective bonding, conventional Universal Dental adhesives require ideal moisture conditions to form a homogeneous adhesive layer, and in turn a strong bond between tooth and restoration. However, when too much or too little moisture is present this may not happen and bonding can fail. Prime&Bond active contains "Active-Guard" technology, which gives this adhesive smart properties that compensate for variations in moisture levels and ensure complete adhesive coverage of the tooth. | | | | | Application Notes are supplied by commercial companies. The Nature Publishing Group does not endorse any of the products or services advertised here. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/myaccount
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