Nature Biotechnology Contents: Volume 36 pp 477 - 554

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Advertisement Do you have a career question? The Naturejobs podcast features one-on-one Q&As, panel discussions and other exclusive content to help scientists with their careers. Hosted on the Naturejobs blog, the podcast is also available on iTunes and Soundcloud.  Listen today! TABLE OF CONTENTS June 2018 Volume 36, Issue 6 Editorials News News Feature Bioentrepreneur Features News and Views Research Letter Careers and Recruitment   Advertisement   Get your all access pass to your next breakthrough The SpectraMax® iD5 five-mode microplate reader measures absorbance, fluorescence, luminescence, TRF and FP. In addition, it can be expanded to include bottom-read luminescence, TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.  Learn more    Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement First Rounders Podcast   Nature Biotechnology's First Rounders podcast is a series of conversations with founders, financers and developers from biotech's past, present and future. Register now Sponsored by: Johns Hopkins University   Editorials   DIYbio gets a poxy rap   p477 doi:10.1038/nbt.4170 Contrary to alarmist headlines, the DIYbio movement is an unlikely biosecurity threat.   Human embryo research policy update   p477 doi:10.1038/nbt.4171 Ethics standards for studies that report human embryo and stem cell research.   News   Mammoth, Arbor and Beam launch new wave of CRISPR startups   pp479 - 480 Cormac Sheridan doi:10.1038/nbt0618-479   Pear approval signals FDA readiness for digital treatments   pp481 - 482 Emily Waltz doi:10.1038/nbt0618-481   Hong Kong stock exchange opens to biotechs   p482 Shannon Ellis doi:10.1038/nbt0618-482   Everything but amyloid: new thinking prompts FDA revamp   pp483 - 484 Eva von Schaper doi:10.1038/nbt0618-483   Next-generation sequencing tests to become routine   p484 Mark Ratner doi:10.1038/nbt0618-484   As CRISPR-Cas adoption soars, summit calls for genome editing oversight   p485 Eric Smalley doi:10.1038/nbt0618-485   Around the world in a month   p486 doi:10.1038/nbt0618-486   Data Page 1Q18—the risk-capital roll continues   p487 Laura DeFrancesco doi:10.1038/nbt.4160   News Feature   Beating the organ clock   pp488 - 492 Melanie Senior doi:10.1038/nbt.4157 Donor organ monitoring and ex vivo perfusion technology are transforming transplantation and offering new therapeutic opportunities. Melanie Senior reports.   Advertisement   Bringing Edman Back to Life Shimadzu's protein sequencers provide reliable, sensitive N-terminal sequencing to researchers through automated Edman degradation. Software enables compliance with FDA 21 CFR Part 11 guidelines, while easy-to-use data analysis functions simplify operation, data processing and reporting. Learn more.   Bioentrepreneur   Data Page Research biotech patenting 2017   pp493 - 494 Brady Huggett and Kathryn Paisner doi:10.1038/nbt.4156   Advertisement   Innovations In: The Biggest Questions in Science Read our new special report on the nature of spacetime, the origin of life, the source of consciousness, and other fundamental questions in science.  Access free online  Produced with support from: The Kavli Prize
Features
Patents
The ethics of access to patented biotech research tools from universities and other research institutions   pp495 - 499 Knut J Egelie, Sabina P Strand, Berit Johansen and Bjørn K Myskja doi:10.1038/nbt.4165 As universities and public research organizations increasingly partner with industry to fulfill their 'third mission' of innovation activities for economic and societal benefit, they have ethical obligations to ensure access to patented research tools, especially CRISPR-Cas9 technology.
Recent patents in metabolomics and metabolic engineering   p500 doi:10.1038/nbt.4168
News and Views
Profiling DNA-transcription factor interactions   pp501 - 502 Cheen Euong Ang and Marius Wernig doi:10.1038/nbt.4161 Global transcription factor activity is measured by screening cell extracts with an oligonucleotide library.   Research Highlights   p502 doi:10.1038/nbt.4159   SCRaMbLE does the yeast genome shuffle   p503 Susan Jones doi:10.1038/nbt.4164   Getting warmer with liver transplants   p504 Irene Jarchum doi:10.1038/nbt.4158   Biotechnology JOBS of the week Postdoctor position in diatom / microalgae biotechnology Norwegian University of Science and Technology Associate Professor Monash University Research Fellow (Bioinformatics) Agency for Science, Technology and Research (A*STAR) Faculty Positions in School of Biotechnology, Jiangnan University Jiangnan University Assistant Professor in Biology / Biotechnology at the Hong Kong Polytechnic University (Ref. 18041901) The Hong Kong Polytechnic University More Science jobs from Biotechnology EVENT Proteins and Peptides: Structure, Function and Biotechnology 23.07.18 Genève, Switzerland More science events from
Research
Brief Communications
Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision   pp505 - 508 Zehua Bao, Mohammad HamediRad, Pu Xue, Han Xiao, Ipek Tasan et al. doi:10.1038/nbt.4132 Efficient genome-scale precision editing in yeast is enabled using CRISPR-Cas9 and homology-directed-repair.
Reversal of siRNA-mediated gene silencing in vivo    pp509 - 511 Ivan Zlatev, Adam Castoreno, Christopher R Brown, June Qin, Scott Waldron et al. doi:10.1038/nbt.4136 Gene silencing by siRNAs in vivo is reversed using sequence-specific high-affinity oligonucleotides.
Articles
Multiplexed precision genome editing with trackable genomic barcodes in yeast   pp512 - 520 Kevin R Roy, Justin D Smith, Sibylle C Vonesch, Gen Lin, Chelsea Szu Tu et al. doi:10.1038/nbt.4137 A method to introduce defined mutations into the yeast genome enables saturation mutagenesis of a gene and genome-scale introduction of genetic variants.
A protein activity assay to measure global transcription factor activity reveals determinants of chromatin accessibility   pp521 - 529 Bei Wei, Arttu Jolma, Biswajyoti Sahu, Lukas M Orre, Fan Zhong et al. doi:10.1038/nbt.4138 A method to analyze global transcription factor activity in cells reveals that a small number of factors determines the landscape of accessible chromatin.
Letter
Photosynthetic artificial organelles sustain and control ATP-dependent reactions in a protocellular system   pp530 - 535 Keel Yong Lee, Sung-Jin Park, Keon Ah Lee, Se-Hwan Kim, Heeyeon Kim et al. doi:10.1038/nbt.4140 Vesicles that convert light energy to ATP are used to power a protocellular system.
Letter
Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy   pp536 - 539 Seuk-Min Ryu, Taeyoung Koo, Kyoungmi Kim, Kayeong Lim, Gayoung Baek et al. doi:10.1038/nbt.4148 Adenine base editing is used to treat a mouse model of Duchenne muscular dystrophy and to create defined mutations in mouse embryos.
Research
Letters
High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR-Cas9 in yeast   pp540 - 546 Xiaoge Guo, Alejandro Chavez, Angela Tung, Yingleong Chan, Christian Kaas et al. doi:10.1038/nbt.4147 An optimized method to introduce precise modifications into the yeast genome enables high-throughput characterization of the phenotypic effect of mutations.
Secure genome-wide association analysis using multiparty computation   pp547 - 551 Hyunghoon Cho, David J Wu and Bonnie Berger doi:10.1038/nbt.4108 A computational protocol built upon modern cryptographic techniques enables secure analysis of large-scale genetic data.
Careers and Recruitment
Optimizing the utility of the individual development plan for trainees in the biosciences   pp552 - 553 Jessica W Tsai, Nathan L Vanderford and Fanuel Muindi doi:10.1038/nbt.4155 Obtaining more data on how institutions, faculty and trainees utilize the individual development plan can only increase its usefulness for exploring both academic and non-academic careers.
People
People   p554 doi:10.1038/nbt.4169
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