The EMBO Journal Table of Contents for 15 May 2018; Vol. 37, No. 10

15 May 2018 | Volume 37, Number 10 Submit Table of Contents Commentary News & Views Articles Resource Volume 37, Number 10 Commentary Informational limits of biological organisms Relatively small genomes orchestrate much more information‐rich phenotypes. This suggests biological systems encode limited precision and efficiency, and that phenotypes are not independent of each other, but co‐determined by the same genomic sequences. J. Taipale argues that it is therefore important to map experimental data back to specific genomic sequences. Jussi Taipale Published online 18.04.2018 News & Views Anti‐cancer therapy: senescence is the new black New findings implicate ECM29‐dependent proteasome hyperactivation in senescence induced by the anti‐breast cancer drug palbociclib. Rodrigo Leite de Oliveira and Rene Bernards Published online 18.04.2018 Articles Mitochondrial DNA and TLR9 drive muscle inflammation upon Opa1 deficiency Mitochondrial DNA stress caused by Opa1 deficiency in skeletal muscle leads to inflammation via TLR9 activation, which contributes to enhanced Fgf21 expression and systemic growth impairment. Aida Rodríguez‐Nuevo, Angels Díaz‐Ramos, Eduard Noguera, Francisco Díaz‐Sáez, Xavier Duran, Juan Pablo Muñoz, Montserrat Romero, Natàlia Plana, David Sebastián, Caterina Tezze, Vanina Romanello, Francesc Ribas, Jordi Seco, Evarist Planet, Susan R Doctrow, Javier González, Miquel Borràs, Marc Liesa, Manuel Palacín, Joan Vendrell, Francesc Villarroya, Marco Sandri, Orian Shirihai and Antonio Zorzano Published online 09.04.2018 Thermal proteome profiling of breast cancer cells reveals proteasomal activation by CDK4/6 inhibitor palbociclib The proteasome is a novel downstream target for the anti‐cancer drug palbociclib inhibiting proliferation by reduced proteasomal association with its inhibitor EMC29. Teemu P Miettinen, Julien Peltier, Anetta Härtlova, Marek Gierliński, Valerie M Jansen, Matthias Trost and Mikael Björklund Published online 18.04.2018 The long non‐coding RNA Paupar promotes KAP1‐dependent chromatin changes and regulates olfactory bulb neurogenesis Complex formation between a long non‐coding RNA (lncRNA), a chromatin regulator and transcription factor illustrates how nuclear lncRNAs can regulate transcription in trans. Ioanna Pavlaki, Farah Alammari, Bin Sun, Neil Clark, Tamara Sirey, Sheena Lee, Dan J Woodcock, Chris P Ponting, Francis G Szele and Keith W Vance Published online 16.04.2018 Real‐time observation of flexible domain movements in CRISPR–Cas9 While crystal structures have provided a static view of Cas9 bound to target DNA, this study uses single‐molecule FRET to illustrate how inter‐domain fluctuations help drive Cas9‐catalyzed DNA cleavage. Saki Osuka, Kazushi Isomura, Shohei Kajimoto, Tomotaka Komori, Hiroshi Nishimasu, Tomohiro Shima, Osamu Nureki and Sotaro Uemura Published online 12.04.2018 Cell type‐specific regulation of ciliary transition zone assembly in vertebrates Synergistic roles of paralogous proteins Rpgrip1 and Rpgrip1l suggest a higher complexity of ciliary transition zone assembly in mouse embryos and human embryonic cells as compared to the simpler hierarchy known from invertebrates. Antonia Wiegering, Renate Dildrop, Lisa Kalfhues, André Spychala, Stefanie Kuschel, Johanna Maria Lier, Thomas Zobel, Stefanie Dahmen, Tristan Leu, Andreas Struchtrup, Flora Legendre, Christine Vesque, Sylvie Schneider‐Maunoury, Sophie Saunier, Ulrich Rüther and Christoph Gerhardt Published online 12.04.2018 Intragenic transcriptional interference regulates the human immune ligand MICA A non‐coding upstream promoter regulates expression of a downstream gene in response to external signalling, demonstrating the existence of promoter interference in mammalian cells. Da Lin, Thomas K Hiron and Christopher A O'Callaghan Published online 11.04.2018 Resource Phosphoproteome dynamics during mitotic exit in budding yeast Ordered target dephosphorylation upon successive inactivation of CDK and Polo kinases is unexpectedly paralleled by a similarly large number of new phosphorylation events via late mitotic kinases. Sandra A Touati, Meghna Kataria, Andrew W Jones, Ambrosius P Snijders and Frank Uhlmann Published online 12.04.2018   Unsubscribe from or edit your subscription for this service. Or by mail: Customer Service * 973 University Avenue * Los Gatos, CA 95032 * U.S.A. Copyright © 2017 by the European Molecular Biology Organisation.