Nature Biotechnology Contents: Volume 36 pp 287 - 372

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TABLE OF CONTENTS April 2018 Volume 36, Issue 4 Editorial News Features News and Views Research Errata Careers and Recruitment   Advertisement nature.com webcasts Nature Research Custom presents a webcast on: Accelerating the Discovery of New Antimicrobial Compounds Date: Thursday, April 26, 2018 This webcast has been produced on behalf of the sponsor who retains sole responsibility for content  Register for FREE  Sponsored by: Labcyte      Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement Nature Index 2018 Japan Some of Japan's smallest institutions are among the most efficient in the production of high quality scientific research, though the decline in Japan's high quality scientific research output continues. This supplement examines reform efforts in light of the country's aim to become a "super-smart" society. Read the full supplement   Advertisement Nature Neuroscience: Poster on Cerebral Organoids Emerging three-dimensional culture methods enable differentiated human stem cells to form into brain organoids or assembloids, which can be used to study evolution, development, and disease.  Available to download free online  Produced with support from  STEMCELL Technologies    Editorial   The long view on sequencing   p287 doi:10.1038/nbt.4125 Ultra-long read, single-molecule nanopore sequencers are beginning to find an expanding set of research applications in both human genomics and pathogenomics.   News   Roche pays $1.9 billion for Flatiron's army of electronic health record curators   pp289 - 290 Justin Petrone doi:10.1038/nbt0418-289   FDA approves stroke-detecting AI software   p290 doi:10.1038/nbt0418-290   New drug for multidrug-resistant HIV   p291 doi:10.1038/nbt0418-291b   Rollout of high-priced cell and gene therapies forces payer rethink   pp291 - 292 Melanie Senior doi:10.1038/nbt0418-291a   Low-molecular-weight albumin drug touted for severe osteoarthritis   p293 Cormac Sheridan doi:10.1038/nbt0418-293   Critics say 'right to try' wrong for patients   pp294 - 295 Christopher Morrison doi:10.1038/nbt0418-294   Around the world in a month   p296 doi:10.1038/nbt0418-296   Advertisement The Trianni Mouse™ platform is the only transgenic antibody discovery platform ever developed that offers the entirety of human antibody variable gene diversity in a single organism. The V-gene segments in The Trianni Mouse are chimeric, but the variable domains of antibodies made by the mouse are entirely human. The result is human antibody leads generated from antibody genes optimized for function in the mouse. Or, in the simplest terms, The Trianni Mouse is a more human mouse.  Learn more    Features   Nature Biotechnology's academic spinouts of 2017   pp297 - 306 Malorye Allison Branca, Ken Garber and Laura DeFrancesco doi:10.1038/nbt.4121 Our annual survey highlights how immune-oncology and screens based on the application of cutting-edge omics technologies are providing a launchpad for a succession of startups interrogating biology across biomedicine.   Patents Gender differences in obtaining and maintaining patent rights   pp307 - 309 Kyle Jensen, Balázs Kovács and Olav Sorenson doi:10.1038/nbt.4120 An examination of the prosecution and maintenance histories of approximately 2.7 million US patent applications indicates that women have less favorable outcomes than men.   Recent patents in tumor detection and treatment   p310 doi:10.1038/nbt.4123   News and Views   Patching the retina with stem cells   pp311 - 313 Kapil Bharti doi:10.1038/nbt.4118 An engineered patch of retinal pigment epithelium shows promise in two macular degeneration patients.   Portable gene expression guaranteed   pp313 - 314 Marios Tomazou and Guy-Bart Stan doi:10.1038/nbt.4119 Synthetic biology is made more reliable by borrowing ideas from systems and control theory.   Research Highlights   p314 doi:10.1038/nbt.4117   Epigenome editing to the rescue   p315 Markus Elsner doi:10.1038/nbt.4116   Biotechnology JOBS of the week Postdoctoral Researcher University of Pennsylvania Post-doctoral fellow Washington University School of Medicine, St. Louis Bioinformatics Specialist Massachusetts General Hospital Epidemiology, Data Science, Statistics and Bioinformatics Faculty Boston University School of Medicine, BUMC Senior Project Manager University of Glasgow More Science jobs from Biotechnology EVENT Medical Biotechnology 24.05.18 Gent, Belgium More science events from Research   Perspective Metabolomics activity screening for identifying metabolites that modulate phenotype   pp316 - 320 Carlos Guijas, J Rafael Montenegro-Burke, Benedikt Warth, Mary E Spilker and Gary Siuzdak doi:10.1038/nbt.4101 Metabolomics activity screening can identify small-molecule metabolites that are readily available and can be used to alter phenotypes, including stem cell differentiation, T-cell survival, oligodendrocyte maturation, and insulin signaling.   Brief Communications Linear assembly of a human centromere on the Y chromosome OPEN   pp321 - 323 Miten Jain, Hugh E Olsen, Daniel J Turner, David Stoddart, Kira V Bulazel et al. doi:10.1038/nbt.4109 Repeat regions in the human Y chromosome centromere are assembled using long-read nanopore sequencing.   Base editing with a Cpf1-cytidine deaminase fusion   pp324 - 327 Xiaosa Li, Ying Wang, Yajing Liu, Bei Yang, Xiao Wang et al. doi:10.1038/nbt.4102 A new fusion protein enables precise editing of single bases in A/T-rich regions of the human genome.   Articles Phase 1 clinical study of an embryonic stem cell-derived retinal pigment epithelium patch in age-related macular degeneration   pp328 - 337 Lyndon da Cruz, Kate Fynes, Odysseas Georgiadis, Julie Kerby, Yvonne H Luo et al. doi:10.1038/nbt.4114 An engineered patch of retinal pigment epithelium generated from human embryonic stem cells is transplanted into the eyes of two patients.   Nanopore sequencing and assembly of a human genome with ultra-long reads OPEN   pp338 - 345 Miten Jain, Sergey Koren, Karen H Miga, Josh Quick, Arthur C Rand et al. doi:10.1038/nbt.4060 A human genome is sequenced and assembled de novo using a pocket-sized nanopore device.   Letters IL-7 and CCL19 expression in CAR-T cells improves immune cell infiltration and CAR-T cell survival in the tumor   pp346 - 351 Keishi Adachi, Yosuke Kano, Tomohiko Nagai, Namiko Okuyama, Yukimi Sakoda et al. doi:10.1038/nbt.4086 Chimeric antigen receptor (CAR)-T cells expressing the cytokines IL-7 and CCL19 show improved clearance of established solid tumors in mice.   Engineered promoters enable constant gene expression at any copy number in bacteria   pp352 - 358 Thomas H Segall-Shapiro, Eduardo D Sontag and Christopher A Voigt doi:10.1038/nbt.4111 A series of feedforward-stabilized bacterial gene promoters produce near-identical expression levels regardless of copy number changes due to altered genetic context, strain or growth conditions.   Resources Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection OPEN   pp359 - 367 Rekha Seshadri, Sinead C Leahy, Graeme T Attwood, Koon Hoong Teh, Suzanne C Lambie et al. doi:10.1038/nbt.4110 Rumen microbiome biology gets a boost with the release of 410 high-quality reference genomes from the Hungate1000 project.   Advertisement Animation on CRISPR: Gene editing and beyond  The CRISPR-Cas9 system has revolutionised gene editing, but cutting DNA isn't all it can do. This Animation, from Nature Methods, explores where CRISPR might be headed next.  Watch the Animation >>  Produced with support from:  Dharmacon    Errata   Erratum: Open-source, community-driven microfluidics with Metafluidics   p368 David S Kong, Todd A Thorsen, Jonathan Babb, Scott T Wick, Jeremy J Gam et al. doi:10.1038/nbt0418-368a   Erratum: The illusion of control in germline engineering policy   p368 Harald Konig doi:10.1038/nbt0418-368b   Erratum: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life   p368 Supratim Mukherjee, Rekha Seshadri, Neha J Varghese, Emiley A Eloe-Fadrosh, Jan P Meier-Kolthoff et al. doi:10.1038/nbt0418-368c   Erratum: Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments   p368 Rajeev K Varshney, Chengcheng Shi, Mahendar Thudi, Cedric Mariac, Jason Wallace et al. doi:10.1038/nbt0418-368d   Erratum: Visualizing detailed postdoctoral employment trends using a new career outcome taxonomy   p368 Hong Xu, Richard S T Gilliam, Shyamal D Peddada, Gregory M Buchold and Tammy R L Collins doi:10.1038/nbt0418-368e   Careers and Recruitment   Involving undergraduates in genomics research to narrow the education-research gap   pp369 - 371 Serghei Mangul, Lana S Martin and Eleazar Eskin doi:10.1038/nbt.4113 Engaging undergraduates in computational tasks can improve genomic research laboratory productivity, benefiting both students and senior laboratory members.   People People   p372 doi:10.1038/nbt.4122     Advertisement Ionis Pharmaceuticals, Nature Structural and Molecular Biology & Nature Biotechnology present: RNA at the Bench & Bedside October 8-10, La Jolla, USA RNA therapeutics is poised to deliver major advances. This conference will discuss the many roles of RNAs and the translation of basic science into new therapies. It will address the opportunities, current status and future of this exciting field.   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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