Nature Biotechnology Contents: Volume 35 pp 389 - 484

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To find out more, visit our website at www.canopybiosciences.com TABLE OF CONTENTS May 2017 Volume 35, Issue 5 Editorial News Bioentrepreneur Opinion and Comment Features News and Views Research Careers and Recruitment Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement nature.com webcasts Springer Nature presents a custom webcast on: Perturb-seq: High Throughput CRISPR Screening via Single-Cell RNA Sequencing Date: Wednesday May 10, 2017 Time: 11AM PDT | 2PM EDT  Register FREE   Sponsored by:  10x Genomics   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement Nature Index Japan 2017  Japan's status as a science superstar is vulnerable. Nature Index 2017 Japan reveals that although the country is still among the upper echelons of global research, its output has continued to slide. Read the full supplement free for six months   Advertisement IBS-NATURE CONFERENCE ON FRONTIERS IN GENOME ENGINEERING  September 27-29, 2017 | Seoul, Korea Presented by: Institute for Basic Science | Institute of Genetics and Developmental Biology, CAS | Nature | Nature Biotechnology REGISTER NOW!   Editorial Top CRISPR catch-up   p389 doi:10.1038/nbt.3885 The hegemony of the CRISPR system as a gene-editing therapy is not as assured as its use as a tool in basic research. News Top First eczema biologic debuts but price could restrict use   pp391 - 392 Elie Dolgin doi:10.1038/nbt0517-391 Genentech's Ocrevus heralds new chapter in MS treatment   pp393 - 394 Cormac Sheridan doi:10.1038/nbt0517-393 Astex shapes CDK4/6 inhibitor for approval   pp395 - 396 Mark Peplow doi:10.1038/nbt0517-395 UC granted CRISPR patents in Europe, appeals in US   p396 doi:10.1038/nbt0517-396 Digital farming attracts cash to agtech startups   pp397 - 398 Emily Waltz doi:10.1038/nbt0517-397 FDA approves PARP inhibitor for ovarian cancer   p398 doi:10.1038/nbt0517-398 Genomics pioneer gets its first drug   p399 Mark Ratner doi:10.1038/nbt0517-399 Data Page Drug pipeline: 1Q17   p400 Laura DeFrancesco doi:10.1038/nbt.3874 News Feature Living in a microbial world   pp401 - 403 Charles Schmidt doi:10.1038/nbt.3868 Our personal ecosystem of microbes is shed on everything we touch and everyplace we go. Charles Schmidt reports on efforts to harness this information to improve human health and activity. Advertisement Faster, Simpler Biological LC-MS/MS Analysis  Shimadzu's Clinical Laboratory Automation Module, a fully integrated sample pretreatment module for LC-MS/MS, automatically performs all of the processes necessary for analyzing blood and other biological samples, providing more precise results by reducing human error and improving laboratory safety and efficiency. Learn more. Bioentrepreneur Top Data Page Academic partnerships 2016   pp404 - 405 Brady Huggett doi:10.1038/nbt.3861 Podcast First Rounders: Rachel Haurwitz   p405 doi:10.1038/nbt.3861 Opinion and Comment Top Correspondence Discovering and linking public omics data sets using the Omics Discovery Index   pp406 - 409 Yasset Perez-Riverol, Mingze Bai, Felipe da Veiga Leprevost, Silvano Squizzato, Young Mi Park et al. doi:10.1038/nbt.3790 Firmiana: towards a one-stop proteomic cloud platform for data processing and analysis   pp409 - 412 Jinwen Feng, Chen Ding, Naiqi Qiu, Xiaotian Ni, Dongdong Zhan et al. doi:10.1038/nbt.3825 Features Top Biotech's wellspring—a survey of the health of the private sector in 2016   pp413 - 420 Stacy Lawrence doi:10.1038/nbt.3867 Investment is skewing early as venture capitalists grapple with a volatile political environment, a hot stock market and a flagging IPO outlook. Patents Who reads patents?   pp421 - 424 Lisa Larrimore Ouellette doi:10.1038/nbt.3864 Biotechnology and chemistry researchers look to the patent literature as a source of technical information more than researchers in other fields, and few researchers are deterred from reading patents by concerns about enhanced legal liability. Recent patents in plant organogenesis   p425 doi:10.1038/nbt.3883 Advertisement IMMUNE PROFILING IN HEALTH AND DISEASE  September 17-19, 2017 | Seattle, WA, USA Presented by: Adaptive Biotechnologies | Nature Immunology | Nature Medicine | Nature Biotechnology REGISTER NOW!   News and Views Top Reprogramming the diseased brain   pp426 - 428 Stephen B Dunnett and Anne E Rosser doi:10.1038/nbt.3869 Direct conversion of astrocytes to dopamine neurons in vivo offers fresh optimism for the development of improved Parkinson's disease therapies. See also: Research by Rivetti di Val Cervo et al. Base editing on the rise   pp428 - 429 Andrew May doi:10.1038/nbt.3871 Research on base editing leads to new enzymes, genome-wide specificity measurements, and edited plants and animals. See also: Research by Kim et al. | Research by Zong et al. | Research by Shimatani et al. | Research by Kim et al. Research Highlights   p429 doi:10.1038/nbt.3872 Genome organization by the slice   p430 Markus Elsner doi:10.1038/nbt.3866 Biotechnology JOBS of the week Faculty and Postdoctoral Positions in Molecular Pathogenesis, The TEDA Institute of Biological Sciences and Biotechnology The TEDA Institute of Biological Sciences and Biotechnology, Nankai University Postdoctoral and PhD positions in Bio- Nano Science University of South Australia (UniSA) Requires Scientific Staff National Agri-Food Biotechnology Institute (NABI) Principal Scientist-Cell Line Development Glenmark Pharmaceuticals S.A. Postdoctoral position Genethon More Science jobs from Biotechnology EVENT International Biotechnology and pharmaceutical Industry Forum December 13, 2017 New Delhi, India More science events from Research Top Brief Communications Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes   pp431 - 434 Brett T Staahl, Madhurima Benekareddy, Claire Coulon-Bainier, Ashwin A Banfal, Stephen N Floor et al. doi:10.1038/nbt.3806 Gene editing in the mouse brain is achieved by injection of Cas9 ribonucleoprotein complexes. Highly efficient RNA-guided base editing in mouse embryos   pp435 - 437 Kyoungmi Kim, Seuk-Min Ryu, Sang-Tae Kim, Gayoung Baek, Daesik Kim et al. doi:10.1038/nbt.3816 Mice with targeted point mutations are generated efficiently using Cas9-cytidine deaminase fusions. See also: News and Views by May Precise base editing in rice, wheat and maize with a Cas9- cytidine deaminase fusion   pp438 - 440 Yuan Zong, Yanpeng Wang, Chao Li, Rui Zhang, Kunling Chen et al. doi:10.1038/nbt.3811 Single DNA base pairs are edited in wheat, rice and maize using a Cas9 nickase fusion protein. See also: News and Views by May Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion   pp441 - 443 Zenpei Shimatani, Sachiko Kashojiya, Mariko Takayama, Rie Terada, Takayuki Arazoe et al. doi:10.1038/nbt.3833 Targeted editing of single base pairs is achieved in monocot rice and dicot tomato using Target-AID (Cas9 activation-induced cytidine deaminase fusion). See also: News and Views by May Articles Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model   pp444 - 452 Pia Rivetti di Val Cervo, Roman A Romanov, Giada Spigolon, Debora Masini, Elisa Martin-Montanez et al. doi:10.1038/nbt.3835 In vivo reprogramming of astrocytes to dopamine neurons improves motor behavior in a mouse model of Parkinson's disease. See also: News and Views by Dunnett & Rosser Large-scale design of robust genetic circuits with multiple inputs and outputs for mammalian cells   pp453 - 462 Benjamin H Weinberg, N T Hang Pham, Leidy D Caraballo, Thomas Lozanoski, Adrien Engel et al. doi:10.1038/nbt.3805 Genetic circuits that are reliable, robust, and scalable are built without the need for optimization using a recombinase-based system. Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions   pp463 - 474 Kyuho Han, Edwin E Jeng, Gaelen T Hess, David W Morgens, Amy Li et al. doi:10.1038/nbt.3834 An improved strategy for large-scale combinatorial CRISPR screening enables the identification of synergistic drug targets for cancer. Letter Genome-wide target specificities of CRISPR RNA-guided programmable deaminases   pp475 - 480 Daesik Kim, Kayeong Lim, Sang-Tae Kim, Sun-heui Yoon, Kyoungmi Kim et al. doi:10.1038/nbt.3852 Base editors composed of Cas9 fused to a deaminase show high specificity in genome-wide analyses. See also: News and Views by May Addendum Addendum: Editorial Expression of Concern: DNA-guided genome editing using the Natronobacterium gregoryi Argonaute   p481 Feng Gao, Xiao Z Shen, Feng Jiang, Yongqiang Wu and Chunyu Han doi:10.1038/nbt0517-481a Errata Erratum: Drugging the gut microbiome   p481 Ken Garber doi:10.1038/nbt0517-481b Erratum: An optical probe of synaptic plasticity   p481 Tal Laviv and Ryohei Yasuda doi:10.1038/nbt0517-481c Corrigenda Corrigendum: Response to [ldquo]Railroading at the FDA[rdquo]   p481 Francesco Muntoni, Sue Fletcher and Steve Wilton doi:10.1038/nbt0517-481d Corrigendum: A FRET sensor enables quantitative measurements of membrane charges in live cells   p481 Yuanqing Ma, Yui Yamamoto, Philip R Nicovich, Jesse Goyette, Jeremie Rossy et al. doi:10.1038/nbt0517-481e Careers and Recruitment Top First-quarter biotech job picture   p482 Michael Francisco doi:10.1038/nbt.3875 People People   p484 doi:10.1038/nbt.3881 Advertisement An open access, online-only, multidisciplinary research journal dedicated to publishing the most important scientific advances in the life sciences, physical sciences, and engineering fields that are facilitated by spaceflight and analogue platforms.   Explore the benefits of submitting your next research article.   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. 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