Tuesday, April 11, 2017

Nature Methods Application Notes e-UPDATE: 11 April 2017

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11 April 2017 
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Robotic Microscopy with the Nikon Ti2 for high-content analysis applications
www.nikon.com >
Robotic Microscopy—a combination of high-content screening methods—enables multivariate experimental approaches with large cell populations and member-level sensitivity. Here we explore how the new Nikon Ti2 line of inverted research microscopes is uniquely suited to Robotic Microscopy applications, focusing on work utilizing induced pluripotent stem cells (iPSCs) as disease models in drug screening.

Real-time visualization of DNA structural transitions under mechanical stress using high-resolution optical tweezers and multi-color fluorescence microscopy
lumicks.com >
Single-molecule force spectroscopy (SMFS) tools are widely used to study structural transitions of DNA during overstretching. However, as many of these tools only provide global information, the exact mechanisms that occur during these transitions remain unclear. Combining SMFS with visualization of local information could resolve this problem. In this application note we will discuss how LUMICKS' C-Trap™ technology combines high-resolution optical tweezers as a SMFS tool with confocal fluorescence microscopy to monitor the transition of double-stranded (ds) DNA to single-stranded (ss) DNA upon applying mechanical stress.

INCELLIS: Perfect tool to qualify the cell labelling before using a high end microscope
www.bertin-instruments.com >
Centrosome is an organelle implicated in cell cycle regulation. During cell division, the two centrosomes serve as anchors to guide the chromosomes and divide them into two equitable batches to form the two daughter cells. An aberrant number of centrosomes in a cell is often associated with cancer. Expression of GFP-centrin recombinant protein into in vitro cell culture allows direct visualization of centriole behavior in living cells in different experimental conditions. Nevertheless, as it is a small organelle, imaging of GFP-centrin protein into centrosome requires high sensitivity in fluorescence.

APD SpectBT: Arduino-based mobile vis-Spectrophotometer
www.a-star.edu.sg >
Quantification of samples is an integral step in numerous biological and chemical experiments. Spectrophotometers are often used together with colorimetric assays that react with the compound of interest in the samples. However, spectrophotometers are often costly, bulky and immobile. To increase the convenience and ease the process of spectrophotometry, the APD SpectBT was developed using the Arduino-based platform to create a smartphone dependent mobile spectrophotometer. Through a Bluetooth® connection, users can use a micro-USB portable spectrophotometer and send the readings to the smartphone app to get visible light absorbance readings conveniently. With the portable SpectBT device and app, users can easily perform spectrophotometry on the go in the field and immediate environments conveniently and rapidly.

The Fast mode for ZEISS LSM 880 with Airyscan: highspeed confocal imaging
www.zeiss.com >
In May 2016, ZEISS announced the next innovation step for laser-scanning microscopy imaging with the introduction of the Fast mode for ZEISS LSM 880 with Airyscan. The new concept combines the Airyscan pinhole-plane detection technology with a new illumination shaping approach, enabling a fourfold increase in image acquisition rates. With the new Fast mode, Airyscan now affords researchers simultaneous access to super-resolution, increased signal-to-noise ratio and increased acquisition speeds without compromise.

MAV-seq: Management, Analysis and Visualization of Sequence Data
www.jax.org >
The increasing amount of heterogeneous Next Generation Sequencing (NGS) data generated today necessitates a robust platform for the efficient data storage, management, pre-processing, quality checking, analysis and visualization. Addressing each of these categories is a tremendous undertaking requiring high-level expertise from various disciplines. The typical genomic research laboratories do not always posses these resources, thus, we have developed a scientific solution to assist researchers dealing with the practical issues associated to the high throughput genomic data. We present MAV-seq as a robust platform, which facilitates the management of samples metadata and NGS data pre-processing, quality assessment and visualization. MAV-seq provides interactive modular data flow, which transparently integrates interoperability, structure, standardization, security, controlled accessibility, management of genomic studies and their sample metadata associated with datasets of enormous size and diversity.

A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources
www.bioind.com >
Human mesenchymal stem cells (hMSCs) are multipotent adult stem cells that can be isolated from various tissues as well as generated in vitro from hESCs and iPS cells. hMSCs have at least trilineage differentiation potential in vitro (into fat, bone, and cartilage cells), and it is part of the Minimal Experimental Criteria for MSC proposed by the International Society for Cellular Therapy (ISCT). In addition, differentiation of hMSCs into specific lineage provides the basis for the use of human MSC in cell therapy applications. Either undifferentiated or differentiated, hMSCs can be used for in vivo implantation into damaged tissue sites. The differentiation potential may differ in relation to the culture condition and the source of hMSCs, and it is still unknown which source should be used for each specific disease.


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