Nature Cell Biology contents: April 2017 Volume 19 Number 4, pp 259 - 407

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Advertisement Complete Cell Solutions for Cancer Research from ATCC ATCC provides scientists with a complete set of solutions for tumor biology, metastasis, and cancer immunology studies. Our new drug screening tools include a high-throughput angiogenesis assay, gene-edited isogenic cells, and PBMCs. Let ATCC supply the cell lines, primary cells, stem cells, and cell-based services you need for your experiments. Learn more >> TABLE OF CONTENTS April 2017 Volume 19, Issue 4 Editorial News and Views Articles Letters Erratum Advertisement CALL FOR ENTRIES!    Now is the time to enter Nikon's Small World Photo and Video Competitions. April 30th is the deadline to submit your images and movies taken through the light microscope. In celebration of Nikon's 100th anniversary, the grand prizewinners will receive $3000 and a trip to Japan. The lucky winners will also receive a private tour of Nikon's glass and lens manufacturing facilities.   For more info, visit enter.nikonsw.com Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement The National Eye Institute (NEI) is sponsoring a challenge to spur development of a robust 3-D retina organoid system for modeling retina diseases and testing new therapies. Do you have what it takes to accelerate the development of new retinal therapies? All expertise welcome! Learn more   Advertisement Nature Outlook: Regenerative Medicine  Sometimes, a drug can remedy a chemical imbalance or surgery can repair a structural failure, but there are times when there is no substitute for replacing a part with human tissue or an entire organ. Rapid advances in regenerative medicine are bringing that possibility closer to reality.  Access the Outlook   Produced with support from  Translational Research Informatics Center(TRI)  Clio, Inc.  Sapporo Medical University  CYBERDYNE,INC.     Advertisement Looking to recruit? Limited time offer from Naturejobs:  Get a 20% discount on our Premium job posting package when you buy before March 31, 2017. Reach a potential audience of 10.8 million users* via nature.com Be seen first by candidates — your position will appear at the top of related searches for 60 days and be displayed prominently on the Naturejobs home page as Job of the Week. Plus appear as 'Featured' in search results Post a job now. * Publisher data 2016     Editorial Top On data availability, reproducibility and reuse   p259 doi:10.1038/ncb3506 Data sharing is an inherent principle of research publication, and information on how data may be accessed is key for the replication and furthering of scientific findings. Here we revisit the policies of Nature Cell Biology on data availability. News and Views Top Demystifying blood stem cell fates   pp261 - 263 Fiona K. Hamey and Berthold Göttgens doi:10.1038/ncb3494 Determining the differentiation potential of stem and progenitor cells is essential for understanding their function, yet our ability to do so is limited by the restrictions of experimental assays. Based on single-cell functional and molecular profiling experiments, a new computational approach shows how lineage commitment may occur in human haematopoiesis. See also: Article by Velten et al. PARL paves the way to apoptosis   pp263 - 265 Naotada Ishihara and Katsuyoshi Mihara doi:10.1038/ncb3504 Although the mitochondrial inner membrane rhomboid peptidase PARL is known to participate in critical signalling cascades, its role in apoptosis has remained unclear. PARL is now shown to process the mitochondrial pro-apoptotic protein Smac (also known as DIABLO) for its subsequent release into the cytosol where it antagonizes XIAP-mediated caspase inhibition to promote apoptosis. See also: Article by Saita et al. Talin gets SHANKed in the fight for integrin activation   pp265 - 267 Paul Atherton and Christoph Ballestrem doi:10.1038/ncb3501 Genetic mutations in the SHANK family of proteins are linked to multiple neuropsychiatric disorders including autism spectrum disorders. A study now elucidates critical roles for SHANK in regulating integrin-mediated cell-extracellular matrix adhesion, by sequestering integrin activators. See also: Article by Lilja et al. Hippo interferes with antiviral defences   pp267 - 269 Natalia Muñoz-Wolf and Ed C. Lavelle doi:10.1038/ncb3502 The Hippo pathway responds to environmental factors including nutrient availability, cell density and substrate stiffness to regulate organ size. This pathway is now shown to also regulate antiviral defence by modulating the TBK1-mediated control of interferon production. See also: Article by Zhang et al. Cell Biology JOBS of the week Cell Biology Scientists interested in Infectious Diseases Immunocore Research Technician II, Cell Biology Fred Hutchinson Cancer Research Center Postdoctoral Scholar in Stem Cell Biology and Endocrinology Stanford University Postdoctoral Fellow in Beta Cell Biology CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Postdoctoral Fellow - Bone Regeneration and Stem Cell Biology New York University (NYU) Medical Center More Science jobs from Cell Biology EVENT Advances in Stem Cell Biology Summer Course 26.06.17 Paris, France More science events from Advertisement The 5th Helmholtz-Nature Medicine DIABETES CONFERENCE  September 17-19, 2017 | Munich, Germany Presented by: Helmholtz Zentrum Munchen | Nature Medicine REGISTER NOW!   Articles Top Human haematopoietic stem cell lineage commitment is a continuous process   pp271 - 281 Lars Velten, Simon F. Haas, Simon Raffel, Sandra Blaszkiewicz, Saiful Islam et al. doi:10.1038/ncb3493 Velten et al. use single-cell transcriptomics and functional data to map the early lineage commitment of human haematopoietic stem cells as a continuous process of cells passing through transitory states rather than demarcating discrete progenitors. See also: News and Views by Hamey & Göttgens Genomic instability during reprogramming by nuclear transfer is DNA replication dependent   pp282 - 291 Gloryn Chia, Judith Agudo, Nathan Treff, Mark V. Sauer, David Billing et al. doi:10.1038/ncb3485 Chia et al. show that developmental arrest after human nuclear transfer is associated with genetic instability, including DNA damage, which occurs during DNA replication and is influenced by the developmental origin of the transferred nucleus. SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras   pp292 - 305 Johanna Lilja, Thomas Zacharchenko, Maria Georgiadou, Guillaume Jacquemet, Nicola De Franceschi et al. doi:10.1038/ncb3487 Lilja et al. find that SHANK proteins inhibit the signalling of Ras and Rap G-proteins by restricting their availability at the plasma membrane. This leads to restricted integrin activation, affecting cell spreading, migration and invasion. See also: News and Views by Atherton & Ballestrem Friction forces position the neural anlage   pp306 - 317 Michael Smutny, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena Ruprecht et al. doi:10.1038/ncb3492 Zebrafish neuroectoderm morphogenesis is influenced by the mesoderm germ layer. Smutny et al. now show that friction forces between cells moving in opposite directions, mediated by E-cadherin adhesion, determine the position of the neural anlage. PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis   pp318 - 328 Shotaro Saita, Hendrik Nolte, Kai Uwe Fiedler, Hamid Kashkar, A. Saskia Venne et al. doi:10.1038/ncb3488 Saita et al. show that PARL cleaves Smac (also known as DIABLO) to generate an IAP-binding motif required for its apoptotic activity, identifying PARL-mediated Smac processing as a pro-apoptotic mitochondrial pathway. See also: News and Views by Ishihara & Mihara Altering the threshold of an excitable signal transduction network changes cell migratory modes   pp329 - 340 Yuchuan Miao, Sayak Bhattacharya, Marc Edwards, Huaqing Cai, Takanari Inoue et al. doi:10.1038/ncb3495 Miao et al. show through imaging and mathematical simulations that changing the activation thresholds in an excitable PIP2-Ras-Rap signal transduction network can alter the types of protrusions formed in migrating Dictyostelium cells. The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells   pp341 - 351 Valerio Donato, Massimo Bonora, Daniele Simoneschi, Davide Sartini, Yasusei Kudo et al. doi:10.1038/ncb3491 Donato et al. show that Fbxo15 targets acetylated KBP for degradation to limit mitochondrial expansion, whereas KBP accumulation promotes mitochondrial biogenesis in a Kif1Bα-dependent manner. Endocytic proteins are partitioned at the edge of the clathrin lattice in mammalian cells   pp352 - 361 Kem A. Sochacki, Andrea M. Dickey, Marie-Paule Strub and Justin W. Taraska doi:10.1038/ncb3498 Using large-scale, super-resolution microscopy, Sochacki et al. define the spatial organization of 19 proteins within clathrin-coated pits during distinct stages of clathrin-mediated endocytosis. Hippo signalling governs cytosolic nucleic acid sensing through YAP/TAZ-mediated TBK1 blockade   pp362 - 374 Qian Zhang, Fansen Meng, Shasha Chen, Steven W. Plouffe, Shiying Wu et al. doi:10.1038/ncb3496 Zhang et al. show that YAP and TAZ suppress nucleic acid sensing and antiviral responses by inhibiting the TBK1 kinase. Conversely, Hippo pathway activity inactivates YAP/TAZ to relieve TBK1 suppression and enhance antiviral defence in zebrafish. See also: News and Views by Munoz-Wolf & Lavelle Letters Top Basolateral protrusion and apical contraction cooperatively drive Drosophila germ-band extension   pp375 - 383 Zijun Sun, Christopher Amourda, Murat Shagirov, Yusuke Hara, Timothy E. Saunders et al. doi:10.1038/ncb3497 Sun et al. show that during Drosophila germ-band extension basolateral rosette formation does not depend on apical contractility, but is driven by Rac1-mediated protrusion and active cell migration and requires Src42A as a regulator. The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation   pp384 - 390 Justin Decarreau, Michael Wagenbach, Eric Lynch, Aaron R. Halpern, Joshua C. Vaughan et al. doi:10.1038/ncb3486 Decarreau and colleagues demonstrate that the minus-directed kinesin Kif25 acts to prevent both premature centrosome separation and mis-positioning of the nucleus and mitotic spindle. Tubulin acetylation protects long-lived microtubules against mechanical ageing   pp391 - 398 Didier Portran, Laura Schaedel, Zhenjie Xu, Manuel Théry and Maxence V. Nachury doi:10.1038/ncb3481 Acetylation of α-tubulin on lysine 40 is associated with microtubule stability. In vitro experiments by Portran et al. show that tubulin acetylation reduces lateral interactions, increasing microtubule flexibility and resistance to mechanical stress. Endoplasmic-reticulum-mediated microtubule alignment governs cytoplasmic streaming   pp399 - 406 Kenji Kimura, Alexandre Mamane, Tohru Sasaki, Kohta Sato, Jun Takagi et al. doi:10.1038/ncb3490 Through imaging and theoretical modelling, Kimura et al. discover that endoplasmic reticulum flow determines microtubule alignment to promote cytoplasmic streaming of yolk granules in Caenorhabditis elegans zygotes. Erratum Top Erratum: Tissue-scale coordination of cellular behaviour promotes epidermal wound repair in live mice   p407 Sangbum Park, David G. Gonzalez, Boris Guirao, Jonathan D. Boucher, Katie Cockburn et al. doi:10.1038/ncb3503 Top Advertisement nature.com webcasts Nature Cell Biology, Nature Reviews Clinical Oncology and the cancer editorial community of the Nature journals present a webcast on: Cancer Evolution Date: Friday, April 28th, 2017 Time: 12:00pm EDT Register for FREE Sponsored by: EMD Serono     Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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