ANGIOGENESIS, CARDIOVASCULAR AND PULMONARY SYSTEMS
The role of endothelial HIF-1 αin the response to sublethal hypoxia in C57BL/6 mouse pups
Using wild-type and endothelial cell (EC)-deficient mice and microvascular ECs, the authors determined that loss of EC HIF-1α affects not only the EC but also the neuronal precursors resident in neurogenic regions of the brain, affecting responsiveness to and recovery from hypoxia and behavior in a gender-specific manner.
Qi Li, Michael Michaud, Chan Park, Yan Huang, Rachael Couture, Frank Girodano, Michael L Schwartz and Joseph A Madri
2017 97: 356-369; advance online publication, January 16, 2017; 10.1038/labinvest.2016.154
Reversal of maladaptive fibrosis and compromised ventricular function in the pressure overloaded heart by a caveolin-1 surrogate peptide
Low caveolin-1 in various cell types results in lung and skin fibrosis. To study caveolin-1′s role in pressure overload-induced heart fibrosis, mice were treated with a caveolin-1 surrogate peptide. The authors found that the peptide suppresses fibrosis and associated signaling and improves ventricular function. Flow cytometry and immunohistochemistry indicate that CD45+/ HSP47+ cells play a key role in heart fibrosis.
Dorea Pleasant-Jenkins, Charles Reese, Panneerselvem Chinnakkannu, Harinath Kasiganesan, Elena Tourkina, Stanley Hoffman and Dhandapani Kuppuswamy
2017 97: 370-382; advance online publication, January 23, 2017; 10.1038/labinvest.2016.153
Pathological changes in basement membranes and dermal connective tissue of skin from patients with hereditary cystatin C amyloid angiopathy
The authors employed skin biopsies from mutation carriers to study intermediate events in hereditary cystatin C amyloid angiopathy (HCCAA) pathogenesis. The results showed an association between increased immunoreactivity of the basement membrane protein COLIV and cystatin C deposition, suggesting that basement membrane changes are early and important events in HCCAA pathogenesis.
Asbjorg Osk Snorradottir, Helgi J Isaksson, Saevar Ingthorsson, Elias Olafsson, Astridur Palsdottir and Birkir Thor Bragason
2017 97: 383-394; advance online publication, January 9, 2017; 10.1038/labinvest.2016.133
Rescue of retinal morphology and function in a humanized mouse at the mouse retinol-binding protein locus
Retinol-binding protein RBP4 is the specific carrier for retinol in the blood. The authors produced a humanized Rbp4hRBP4/ hRBP4 mouse in which the serum hRBP4 level is approximately 30% of that in Rbp4+/+ mice. The electroretinogram and morphological abnormalities observed in Rbp4-/- animals are not detected in the humanized mice.
Li Liu, Tomohiro Suzuki, Jingling Shen, Shigeharu Wakana, Kimi Araki, Ken-ichi Yamamura, Lei Lei and Zhenghua Li
2017 97: 395-408; advance online publication, January 30, 2017; 10.1038/labinvest.2016.156
GDM alters paracrine regulation of feto-placental angiogenesis via the trophoblast
The trophoblast is a rich source of secreted pro-and anti-angiogenic factors. Gestational diabetes (GDM) alters feto-placental vascular architecture. The authors hypothesized that GDM modulates paracrine regulation of feto-placental angiogenesis by the trophoblast and investigated the composition and effect of medium conditioned by trophoblast from normal and GDM pregnancies on feto-placental endothelial cells.
Jelena Loegl, Erika Nussbaumer, Silvija Cvitic, Berthold Huppertz, Gernot Desoye and Ursula Hiden
2017 97: 409-418; advance online publication, January 23, 2017; 10.1038/labinvest.2016.149
APX-115, a first-in-class pan-NADPH oxidase (Nox) inhibitor, protects db/db mice from renal injury
In this study, the authors aimed to ameliorate renal damage from reactive oxygen species in diabetic nephropathy. They found that a recently developed pan-Nox-inhibitor, APX-115, may provide better renoprotection than the dual Nox1/Nox4 inhibitor. Pan-Nox inhibition with APX-115 might be a promising therapy for diabetic nephropathy and targeting all Nox components may be a new therapeutic strategy
Jin Joo Cha, Hye Sook Min, Ki Tae Kim, Jung Eun Kim, Jung Yeon Ghee, Hyun Wook Kim, Ji Eun Lee, Jee Young Han, Gayoung Lee, Hun Joo Ha, Yun Soo Bae, Sae Rom Lee, Sung Hwan Moon, Sung Chan Lee, Ganghyun Kim, Young Sun Kang and Dae Ryong Cha
2017 97: 419-431; advance online publication, February 6, 2017; 10.1038/labinvest.2017.2
Sodium chloride promotes tissue inflammation via osmotic stimuli in subtotal-nephrectomized mice
Chronic inflammation, which is often associated with high cardiovascular mortality, is prevalent in patients with renal failure; however, the precise mechanisms remain unclear. This study indicates that high NaCl intake induces monocyte chemotactic protein-1 expression leading to macrophage infiltration, and that tonicity-responsive enhancer binding protein plays a central role in inflammation in patients with renal failure who consume a high-salt diet.
Long noncoding RNA FTX is upregulated in gliomas and promotes proliferation and invasion of glioma cells by negatively regulating miR-342-3p
In this study, the authors found that the long non-coding RNA FTX, in conjunction with the microRNA miR-342-3p, promotes proliferation and invasion of glioma cells. FTX inhibits expression of miR-342-3p, which targets the 3′UTR of astrocyte gene-1 mRNA. The subsequent decrease in astrocyte gene-1 protein levels may promote glioma cell growth and epithelial-mesenchymal transition. FTX and miR-342-3p may therefore serve as biomarkers for glioma diagnosis, and are potential therapeutic targets.
Weiguang Zhang, Yunke Bi, Jianhua Li, Fei Peng, Hui Li, Chenguang Li, Laizang Wang, Fubin Ren, Chen Xie, Pengwei Wang, Weiwei Liang, Zhi Wang and Dan Zhu
2017 97: 447-457; advance online publication, January 23, 2017; 10.1038/labinvest.2016.152
Pathogenic role of endogenous TNF-α in the development of Sjögren’s-like sialadenitis and secretory dysfunction in non-obese diabetic mice
This study shows that inhibition of endogenous TNF-αa activity with a neutralizing antibody impedes the development of Sjögren's-like salivary gland inflammation and secretory dysfunction in female non-obese diabetic mice. The authors conclude that endogenous TNF-a may play a critical role in the development and onset of Sjögren's disease.
Jing Zhou, Toshihisa Kawai and Qing Yu
2017 97: 458-467; advance online publication, January 9, 2017; 10.1038/labinvest.2016.141
High-pressure carbon monoxide preserves rat kidney grafts from apoptosis and inflammation
The authors investigated a new strategy for organ preservation using ex vivo application of high-pressure carbon monoxide (CO) and oxygen in rat kidney transplantation model. High-pressure CO significantly improves graft function compared with simple cold storage using University of Wisconsin solution by protecting tubular epithelial cells from inflammation and apoptosis.
Regeneration of thyroid follicles from primordial cells in a murine thyroidectomized model
To gain insight into follicle regeneration in the adult thyroid gland, the authors observed the regeneration processes of murine thyroid follicles after partial resection. They found that the follicle regeneration process associated with the appearance of primitive follicular cells may be a platform for the budding of differentiated follicles in mice.
Junguee Lee, Shinae Yi, Joon Young Chang, Yea Eun Kang, Hyun Jung Kim, Ki Cheol Park, Keum-Jin Yang, Hae Joung Sul, Jong Ok Kim, Hyon-Seung Yi, Xuguang Zhu, Sheue-yann Cheng and Minho Shong
2017 97: 478-489; advance online publication, January 23, 2017; 10.1038/labinvest.2016.158
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