Laboratory Investigation - Table of Contents alert Volume 97 Issue 1

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TABLE OF CONTENTS Volume 97, Issue 1 (January 2017) In this issue Inside the USCAP Journals Pathobiology In Focus Research Articles Technical Reports Also new AOP Sign up for e-alerts Recommend to your library Web feed Subscribe Inside the USCAP Journals Top Inside the USCAP Journals 2017 97: 2-3; 10.1038/labinvest.2016.136 Full Text Pathobiology In Focus Top CD68/macrosialin: not just a histochemical marker CD68 is not restricted to serving as a cytochemical marker of monocyte/macrophages. CD68 is involved in binding several ligands, such as oxidized low density lipoproteins, phosphatidylserine, apoptotic cells and malaria sporozoite. However, CD68 it is not involved in binding bacterial/viral pathogens, innate, inflammatory or humoral immune responses. Dimitry A Chistiakov, Murry C Killingsworth, Veronika A Myasoedova, Alexander N Orekhov and Yuri V Bobryshev 2017 97: 4-13; advance online publication, November 21, 2016; 10.1038/labinvest.2016.116 Abstract | Full Text Research Articles Top ORAL AND GASTROINTESTINAL SYSTEMS Gluten-induced symptoms in diarrhea-predominant irritable bowel syndrome are associated with increased myosin light chain kinase activity and claudin-15 expression The mechanisms underlying diarrhea-predominant irritable bowel syndrome (IBS-D) are poorly understood, though a gluten-free diet has demonstrated symptomatic improvement. This study shows that alterations in myosin II regulatory light chain phosphorylation and claudin-15 and claudin-2 expression are associated with gluten-induced symptomatology and intestinal permeability changes in IBS-D. Richard L Wu, Maria I Vazquez-Roque, Paula Carlson, Duane Burton, Madhusudan Grover, Michael Camilleri and Jerrold R Turner 2017 97: 14-23; advance online publication, November 21, 2016; 10.1038/labinvest.2016.118 Abstract | Full Text ANGIOGENESIS, CARDIOVASCULAR AND PULMONARY SYSTEMS Longitudinal microcomputed tomography-derived biomarkers for lung metastasis detection in a syngeneic mouse model: added value to bioluminescence imaging Micro-computed tomography efficiently, non-invasively and repeatedly monitors metastasis to the lung in free-breathing syngeneic mice. This method thereby allows visualization of tumor morphology and various biomarkers that quantify not only tumor load, but also aerated space in the lung, reflecting vital lung capacity and potential compensatory mechanisms in mouse models of lung metastasis. Eyra Marien, Amy Hillen, Frank Vanderhoydonc, Johannes V Swinnen and Greetje Vande Velde 2017 97: 24-33; advance online publication, November 21, 2016; 10.1038/labinvest.2016.114 Abstract | Full Text The receptor for advanced glycation end products impairs collateral formation in both diabetic and non-diabetic mice Diabetics often have poor perfusion in their limbs as a result of peripheral artery disease and impaired ability to generate collateral vessels. This study reveals a role of the receptor for advanced glycation end products (RAGE) in collateral formation using a mouse model. Mechanistically, activation of RAGE by ligands, including AGE and the chromatin protein high mobility group box 1, decreases the ability to form compensatory collaterals. Laura M Hansen, Divya Gupta, Giji Joseph, Daiana Weiss and W Robert Taylor 2017 97: 34-42; advance online publication, November 21, 2016; 10.1038/labinvest.2016.113 Abstract | Full Text ENDOCRINE, VISUAL AND AUDITORY SYSTEMS Protective effects of an HTRA1 insertion–deletion variant against age-related macular degeneration in the Chinese populations This study describes the genetic and molecular association of a protective HTRA1 insertion-deletion variant (c.34delCinsTCCT) with age-related macular degeneration in Chinese populations. It also reveals that elevated levels of the serine protease HTRA1 induces retinal pigment epithelial cell death, suggesting a potential mechanism for the pathogenesis of age-related macular degeneration. Tsz Kin Ng, Xiao Ying Liang, Fang Lu, David TL Liu, Gary HF Yam, Li Ma, Pancy OS Tam, Haoyu Chen, Ling Ping Cen, Li Jia Chen, Zhenglin Yang and Chi Pui Pang 2017 97: 43-52; advance online publication, November 14, 2016; 10.1038/labinvest.2016.117 Abstract | Full Text HEPATIC AND PANCREATIC SYSTEMS ASIC1a mediates the drug resistance of human hepatocellular carcinoma via the Ca2+/PI3-kinase/AKT signaling pathway OPEN This paper shows that acid-sensing ion channel 1 (ASIC1a) mediates the drug resistance of human hepatocellular carcinoma (HCC) through the Ca2+/PI3K/AKT signaling pathway in the extracellular acidic microenvironment. These data suggest a novel pathway that regulates drug resistance, thus offering a potential target for treatment of HCC. Yihao Zhang, Ting Zhang, Chao Wu, Quan Xia and Dujuan Xu 2017 97: 53-69; advance online publication, December 5, 2016; 10.1038/labinvest.2016.127 Abstract | Full Text Technical Reports Top MODELS AND TECHNIQUES An optimized protocol for purification of functional islets of Langerhans This study presents a procedure for reproducible isolation and evaluation of normal and diseased pancreatic islets. The authors present islet quality tests and functional assessments as standard checkpoints for studies relating to beta cells. As proof of concept, this approach was used to uncover a new ion channel candidate implicated in insulin secretion, transient receptor potential canonical channels. Youakim Saliba, Jules-Joel Bakhos, Tarek Itani and Nassim Farès 2017 97: 70-83; advance online publication, November 28, 2016; 10.1038/labinvest.2016.123 Abstract | Full Text Automated evaluation of liver fibrosis in thioacetamide, carbon tetrachloride, and bile duct ligation rodent models using second-harmonic generation/two-photon excited fluorescence microscopy Precise staging of liver fibrosis is important for assessment of treatment and determining the efficacy of potential new drugs. An automated evaluation system using second harmonic generation/two photon excited fluorescence microscopy was developed by combing eleven shared and model-specific parameters. This new protocol can specifically, accurately, and quantitatively stage liver fibrosis in animal models. Feng Liu, Long Chen, Hui-Ying Rao, Xiao Teng, Ya-Yun Ren, Yan-Qiang Lu, Wei Zhang, Nan Wu, Fang-Fang Liu and Lai Wei 2017 97: 84-92; advance online publication, December 5, 2016; 10.1038/labinvest.2016.128 Abstract | Full Text Microfluidics-assisted fluorescence in situ hybridization for advantageous human epidermal growth factor receptor 2 assessment in breast cancer A novel technique, microfluidics-assisted fluorescence in situ hybridization (MA-FISH), based on oscillatory microfluidic recirculation of DNA probes for HER2 classification in fixed breast cancer tissue, is described. MA-FISH offers similar diagnostic capability as the standard technique, but dramatically reduces the volumes of reagents and analysis times, hence facilitating the dissemination of the technique. Huu Tuan Nguyen, Raphaël Trouillon, Seiya Matsuoka, Maryse Fiche, Laurence de Leval, Bettina Bisig and Martin AM Gijs 2017 97: 93-103; advance online publication, November 28, 2016; 10.1038/labinvest.2016.121 Abstract | Full Text Covalently deposited dyes: a new chromogen paradigm that facilitates analysis of multiple biomarkers in situ A new paradigm in chromogen chemistry is demonstrated using tyramine as a common activable linker unit for rapid creation of new dyes, compatible with routine bright field microscopy. These unique compounds have selectable spectral properties useful for multiplexed biomarker analysis of protein and nucleic acid targets. William A Day, Mark R Lefever, Robert L Ochs, Anne Pedata, Lauren J Behman, Julia Ashworth-Sharpe, Donald D Johnson, Eric J May, James G Grille, Esteban A Roberts, Jerry W Kosmeder and Larry E Morrison 2017 97: 104-113; advance online publication, November 21, 2016; 10.1038/labinvest.2016.115 Abstract | Full Text Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department. For print subscription enquiries, please contact our subscription department. 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