Wednesday, January 4, 2017

Laboratory Investigation - Table of Contents alert Volume 97 Issue 1

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Laboratory Investigation


Volume 97, Issue 1 (January 2017)

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Inside the USCAP Journals
Pathobiology In Focus
Research Articles
Technical Reports

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Inside the USCAP Journals


Inside the USCAP Journals

2017 97: 2-3; 10.1038/labinvest.2016.136

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Pathobiology In Focus


CD68/macrosialin: not just a histochemical marker

CD68 is not restricted to serving as a cytochemical marker of monocyte/macrophages. CD68 is involved in binding several ligands, such as oxidized low density lipoproteins, phosphatidylserine, apoptotic cells and malaria sporozoite. However, CD68 it is not involved in binding bacterial/viral pathogens, innate, inflammatory or humoral immune responses.

Dimitry A Chistiakov, Murry C Killingsworth, Veronika A Myasoedova, Alexander N Orekhov and Yuri V Bobryshev

2017 97: 4-13; advance online publication, November 21, 2016; 10.1038/labinvest.2016.116

Abstract | Full Text

Research Articles



Gluten-induced symptoms in diarrhea-predominant irritable bowel syndrome are associated with increased myosin light chain kinase activity and claudin-15 expression

The mechanisms underlying diarrhea-predominant irritable bowel syndrome (IBS-D) are poorly understood, though a gluten-free diet has demonstrated symptomatic improvement. This study shows that alterations in myosin II regulatory light chain phosphorylation and claudin-15 and claudin-2 expression are associated with gluten-induced symptomatology and intestinal permeability changes in IBS-D.

Richard L Wu, Maria I Vazquez-Roque, Paula Carlson, Duane Burton, Madhusudan Grover, Michael Camilleri and Jerrold R Turner

2017 97: 14-23; advance online publication, November 21, 2016; 10.1038/labinvest.2016.118

Abstract | Full Text


Longitudinal microcomputed tomography-derived biomarkers for lung metastasis detection in a syngeneic mouse model: added value to bioluminescence imaging

Micro-computed tomography efficiently, non-invasively and repeatedly monitors metastasis to the lung in free-breathing syngeneic mice. This method thereby allows visualization of tumor morphology and various biomarkers that quantify not only tumor load, but also aerated space in the lung, reflecting vital lung capacity and potential compensatory mechanisms in mouse models of lung metastasis.

Eyra Marien, Amy Hillen, Frank Vanderhoydonc, Johannes V Swinnen and Greetje Vande Velde

2017 97: 24-33; advance online publication, November 21, 2016; 10.1038/labinvest.2016.114

Abstract | Full Text

The receptor for advanced glycation end products impairs collateral formation in both diabetic and non-diabetic mice

Diabetics often have poor perfusion in their limbs as a result of peripheral artery disease and impaired ability to generate collateral vessels. This study reveals a role of the receptor for advanced glycation end products (RAGE) in collateral formation using a mouse model. Mechanistically, activation of RAGE by ligands, including AGE and the chromatin protein high mobility group box 1, decreases the ability to form compensatory collaterals.

Laura M Hansen, Divya Gupta, Giji Joseph, Daiana Weiss and W Robert Taylor

2017 97: 34-42; advance online publication, November 21, 2016; 10.1038/labinvest.2016.113

Abstract | Full Text


Protective effects of an HTRA1 insertion–deletion variant against age-related macular degeneration in the Chinese populations

This study describes the genetic and molecular association of a protective HTRA1 insertion-deletion variant (c.34delCinsTCCT) with age-related macular degeneration in Chinese populations. It also reveals that elevated levels of the serine protease HTRA1 induces retinal pigment epithelial cell death, suggesting a potential mechanism for the pathogenesis of age-related macular degeneration.

Tsz Kin Ng, Xiao Ying Liang, Fang Lu, David TL Liu, Gary HF Yam, Li Ma, Pancy OS Tam, Haoyu Chen, Ling Ping Cen, Li Jia Chen, Zhenglin Yang and Chi Pui Pang

2017 97: 43-52; advance online publication, November 14, 2016; 10.1038/labinvest.2016.117

Abstract | Full Text


ASIC1a mediates the drug resistance of human hepatocellular carcinoma via the Ca2+/PI3-kinase/AKT signaling pathway OPEN

This paper shows that acid-sensing ion channel 1 (ASIC1a) mediates the drug resistance of human hepatocellular carcinoma (HCC) through the Ca2+/PI3K/AKT signaling pathway in the extracellular acidic microenvironment. These data suggest a novel pathway that regulates drug resistance, thus offering a potential target for treatment of HCC.

Yihao Zhang, Ting Zhang, Chao Wu, Quan Xia and Dujuan Xu

2017 97: 53-69; advance online publication, December 5, 2016; 10.1038/labinvest.2016.127

Abstract | Full Text

Technical Reports



An optimized protocol for purification of functional islets of Langerhans

This study presents a procedure for reproducible isolation and evaluation of normal and diseased pancreatic islets. The authors present islet quality tests and functional assessments as standard checkpoints for studies relating to beta cells. As proof of concept, this approach was used to uncover a new ion channel candidate implicated in insulin secretion, transient receptor potential canonical channels.

Youakim Saliba, Jules-Joel Bakhos, Tarek Itani and Nassim Farès

2017 97: 70-83; advance online publication, November 28, 2016; 10.1038/labinvest.2016.123

Abstract | Full Text

Automated evaluation of liver fibrosis in thioacetamide, carbon tetrachloride, and bile duct ligation rodent models using second-harmonic generation/two-photon excited fluorescence microscopy

Precise staging of liver fibrosis is important for assessment of treatment and determining the efficacy of potential new drugs. An automated evaluation system using second harmonic generation/two photon excited fluorescence microscopy was developed by combing eleven shared and model-specific parameters. This new protocol can specifically, accurately, and quantitatively stage liver fibrosis in animal models.

Feng Liu, Long Chen, Hui-Ying Rao, Xiao Teng, Ya-Yun Ren, Yan-Qiang Lu, Wei Zhang, Nan Wu, Fang-Fang Liu and Lai Wei

2017 97: 84-92; advance online publication, December 5, 2016; 10.1038/labinvest.2016.128

Abstract | Full Text

Microfluidics-assisted fluorescence in situ hybridization for advantageous human epidermal growth factor receptor 2 assessment in breast cancer

A novel technique, microfluidics-assisted fluorescence in situ hybridization (MA-FISH), based on oscillatory microfluidic recirculation of DNA probes for HER2 classification in fixed breast cancer tissue, is described. MA-FISH offers similar diagnostic capability as the standard technique, but dramatically reduces the volumes of reagents and analysis times, hence facilitating the dissemination of the technique.

Huu Tuan Nguyen, Raphaël Trouillon, Seiya Matsuoka, Maryse Fiche, Laurence de Leval, Bettina Bisig and Martin AM Gijs

2017 97: 93-103; advance online publication, November 28, 2016; 10.1038/labinvest.2016.121

Abstract | Full Text

Covalently deposited dyes: a new chromogen paradigm that facilitates analysis of multiple biomarkers in situ

A new paradigm in chromogen chemistry is demonstrated using tyramine as a common activable linker unit for rapid creation of new dyes, compatible with routine bright field microscopy. These unique compounds have selectable spectral properties useful for multiplexed biomarker analysis of protein and nucleic acid targets.

William A Day, Mark R Lefever, Robert L Ochs, Anne Pedata, Lauren J Behman, Julia Ashworth-Sharpe, Donald D Johnson, Eric J May, James G Grille, Esteban A Roberts, Jerry W Kosmeder and Larry E Morrison

2017 97: 104-113; advance online publication, November 21, 2016; 10.1038/labinvest.2016.115

Abstract | Full Text

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