Nature Methods Application Notes e-UPDATE: 8 November 2016

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8 November 2016  Application Notes bring you the latest information about innovative tools and technologies and their applications in the lab. We hope that you will find this service useful and informative and encourage you to sign up for future Updates to ensure that you never miss one!

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FEATURED APPLICATION NOTE The Fast mode for ZEISS LSM 880 with Airyscan: highspeed confocal imaging www.zeiss.com > In May 2016, ZEISS announced the next innovation step for laser-scanning microscopy imaging with the introduction of the Fast mode for ZEISS LSM 880 with Airyscan. The new concept combines the Airyscan pinhole-plane detection technology with a new illumination shaping approach, enabling a fourfold increase in image acquisition rates. With the new Fast mode, Airyscan now affords researchers simultaneous access to super-resolution, increased signal-to-noise ratio and increased acquisition speeds without compromise. PDF

		Non-invasive impedance monitoring of contractility in Axol Human iPSC-Derived Cardiomyocytes www.axolbio.com/ > The ability to monitor cardiomyocyte beat rate in real time is a powerful tool for drug discovery research, particularly when used in conjunction with human induced pluripotent stem cell (iPSC)-derived cells. This offers a physiologically relevant model in which to reliably assess cardiac liability, enabling lead compounds to be identified sooner, thereby reducing the rate of drug attrition and adverse reactions such as proarrythmias. To do this, human iPSC-derived cardiomyocytes (iPSC-CMs) (Axol Bioscience Ltd.) were cultured in a non-invasive impedance monitoring system (xCELLigence®) to assess cardiotoxicity and cell contractility in a 96-well plate format. PDF

		Recent developments in FEI's in situ cryo-electron www.fei.com/ > Studying the molecular machinery of cells from atomic details to the cellular context and beyond is a great challenge for cell biology. It requires the integration of dynamic and structural information obtained using different ranges of spatial resolution. In order for this to be accomplished, a comprehensive workflow is needed covering vitreous freezing, cryo-fluorescence microscopy, sample thinning by focused ion beam microscopy, and high-resolution cryo-electron tomography. This Application Note provides an overview of FEI's integrated product suite, providing a complete cryo-transmission electron microscopy (cryo-TEM) tomography workflow. PDF

		DNA fragmentation and quality control analysis using Diagenode shearing systems and Fragment Analyzer www.diagenode.com/ > www.aati-us.com/ > Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, namely, precise DNA fragmentation, size-range analysis, and smear quantification. The Diagenode One, Bioruptor®, Megaruptor®, and Fragment Analyzer™ by Advanced Analytical ensure that these first critical steps generate quality results. PDF

		Automated NGS library preparation on CyBi-FeliX www.cybio-ag.com/ > Automation of library preparation is very important to reduce opportunities for error, increase reproducibility, and reduce the amount ofhands-on time allowing researchers to generate sequence data from DNA more efficiently. By utilizing the CyBi®-FeliX, a benchtop instrument for a variety of automated liquid handling tasks, the HaloPlex Target EnrichmentSystem Kit for NGS library preparation for Ion Torrent™ Sequencing was automated to achieve reliable final prepared libraries with high accuracy for downstream sequencing. This application note describes the successful proof of principle to generate high-quality DNA libraries on CyBi®-FeliX for personal genome analysis with the Ion Torrent™ Sequencing System. PDF

		Advances in Flavivirus research applications: new techniques using the FastPrep-24 5G sample prep system www.mpbio.com/ > The FastPrep-24 5G is the most innovative bead-beating sample-preparation instrument and produces quantitative, thorough and rapid grinding, lysis and homogenization of routine and resistant samples. PDF

		A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources www.bioind.com/ > Human mesenchymal stem cells (hMSCs) are multipotent adult stem cells that can be isolated from various tissues as well as generated in vitro from hESCs and iPS cells. hMSCs have at least trilineage differentiation potential in vitro (into fat, bone, and cartilage cells), and it is part of the Minimal Experimental Criteria for MSC proposed by the International Society for Cellular Therapy (ISCT). In addition, differentiation of hMSCs into specific lineage provides the basis for the use of human MSC in cell therapy applications. Either undifferentiated or differentiated, hMSCs can be used for in vivo implantation into damaged tissue sites. The differentiation potential may differ in relation to the culture condition and the source of hMSCs, and it is still unknown which source should be used for each specific disease. PDF

 

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