Nature Structural & Molecular Biology Contents: 2016 Volume #23 pp 871-948

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Advertisement Need access to Nature Plants at your workplace or institution? Site license access offers full online content plus added benefits! Recommend site license access to your librarian.  TABLE OF CONTENTS October 2016 Volume 23, Issue 10 Editorial News and Views Articles Advertisement nature.com webcasts Springer Nature presents a custom webcast on Advancements in single-cell RNA-seq: differential expression analysis and immune profiling November 3, 2016; 9AM PDT, 12PM EDT, 4PM GMT, 5PM CET Learn about the latest advancements in single-cell RNA-seq and its application in whole transcriptome analysis and immune profiling. Register for FREE Sponsored by  Takara Bio USA, Inc. Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement nature.com webcasts Springer Nature presents a custom webcast on: Optimizing sensitivity and specificity in the RT-QuIC assay using BMG plate readers Date: Wednesday October 19, 2016 Time: 8AM PDT, 11AM EDT, 4PM BST, 5PM CEST  Register for FREE at   And learn how the RT-QuIC assay is currently being used as a diagnostic in the clinical setting for CJD and has the potential to be a valuable screening tool. Sponsored by: BMG LABTECH     Advertisement Cell Death & Disease is an online-only, open access journal seeking to promote diverse and integrated areas of experimental and internal medicine with its specialities, including cancer, cancer metabolism, immunity and neuroscience. Explore the benefits of submitting your next research article.   Editorial Top Where are the data?   p871 doi:10.1038/nsmb.3307 Here, we announce two policy changes across Nature journals: data-availability statements in all published papers and official Worldwide Protein Data Bank (wwPDB) validation reports for peer review. News and Views Top Structural basis for the poisonous activity of a predator's venom insulin   pp872 - 874 Pierre De Meyts doi:10.1038/nsmb.3304 A potent toxin present in the venom of a fish-hunting cone snail is a minimized insulin (Con-Ins G1) lacking key residues involved in the receptor binding of most insulins. New data show that Con-Ins G1 nevertheless binds potently to the human insulin receptor, owing to a rearrangement that compensates for the lack of a critical binding residue. See also: Article by Menting et al. Replicating methicillin resistance?   pp874 - 875 Joshua P Ramsay doi:10.1038/nsmb.3303 Methicillin resistance in the clinically important bacterium Staphylococcus aureus (MRSA) has evolved in multiple S. aureus lineages through acquisition of chromosomally integrating mobile genetic elements named SCCmec. Now Rice and colleagues show that the conserved SCCmec cch gene encodes an active DNA helicase, thus suggesting that extrachromosomal replication is part of the enigmatic SCCmec horizontal-transfer mechanism. See also: Article by Mir-Sanchis et al. Structural & Molecular Biology JOBS of the week Research Associate King's College London Chair of the Department of Biochemistry and Molecular Biology Oregon Health & Science University (OHSU) School of Medicine Assistant Professor University of Colorado Anschutz Medical Campus Postdoctoral Position in Chemical Biology of Tubulin Modifications Ecole Polytechnique Fédérale de Lausanne (EPFL) More Science jobs from Advertisement Nature Insight: The Protein World This Insight highlights four exciting topics in contemporary protein science: de novo designed proteins; how cells monitor and regulate the proteome; the rise of cryo-electron microscopy; and proteome analysis through high-resolution mass spectrometry.     Articles Top Protecting genome integrity during CRISPR immune adaptation   pp876 - 883 Addison V Wright and Jennifer A Doudna doi:10.1038/nsmb.3289 Cas1-Cas2 integrase achieves full-site integration only for proper targets and protospacers, whereas at non-CRISPR sites, integration stalls at the half-site intermediate, thereby protecting host genome integrity during CRISPR immune adaptation. Membrane insertion of a Tc toxin in near-atomic detail   pp884 - 890 Christos Gatsogiannis, Felipe Merino, Daniel Prumbaum, Daniel Roderer, Franziska Leidreiter et al. doi:10.1038/nsmb.3281 A cryo-EM structure of toxin component TcdA1 embedded in lipid nanodiscs reveals details of the mechanism used by this bacterial toxin to insert into the host cell membrane. Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative   pp891 - 898 Ignacio Mir-Sanchis, Christina A Roman, Agnieszka Misiura, Ying Z Pigli, Susan Boyle-Vavra et al. doi:10.1038/nsmb.3286 One of the conserved proteins of the Staphylococcus aureus mobile genomic island responsible for methicillin resistance is an active MCM-like helicase, thus suggesting replication that would enhance the efficiency of horizontal gene transfer. See also: News and Views by Ramsay Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy   pp899 - 905 Alexandra C Walls, M Alejandra Tortorici, Brandon Frenz, Joost Snijder, Wentao Li et al. doi:10.1038/nsmb.3293 Cryo-EM and mass spectrometry analyses of the spike glycoprotein trimer from coronavirus HcoV-NL63 reveal an extensive glycan shield that covers the protein surface, including an epitope targeted by neutralizing antibodies against several coronaviruses. Natively glycosylated HIV-1 Env structure reveals new mode for antibody recognition of the CD4-binding site   pp906 - 915 Harry B Gristick, Lotta von Boehmer, Anthony P West Jr, Michael Schamber, Anna Gazumyan et al. doi:10.1038/nsmb.3291 Crystal structures of HIV Env trimer with native glycosylation in complex with neutralizing antibodies reveal a glycan shield of high-mannose and complex-type N-glycan and indicate a path for germline-targeting vaccine design. A minimized human insulin-receptor-binding motif revealed in a Conus geographus venom insulin   pp916 - 920 John G Menting, Joanna Gajewiak, Christopher A MacRaild, Danny Hung-Chieh Chou, Maria M Disotuar et al. doi:10.1038/nsmb.3292 Structural elucidation and biochemical analysis of a cone snail insulin venom that binds and activates the human insulin receptor may permit design of ultrafast-acting insulin analogs for diabetes therapy. See also: News and Views by De Meyts A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex   pp921 - 932 Thomas Clairfeuille, Caroline Mas, Audrey S M Chan, Zhe Yang, Maria Tello-Lafoz et al. doi:10.1038/nsmb.3290 A systematic analysis reveals that acidic or phosphorylated residues upstream of the PDZ-binding motif contribute to efficient recognition of cargos by the SNX27 PDZ domain, thus leading to the identification of hundreds of potential new SNX27 ligands. The DDB1-DCAF1-Vpr-UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction   pp933 - 940 Ying Wu, Xiaohong Zhou, Christopher O Barnes, Maria DeLucia, Aina E Cohen et al. doi:10.1038/nsmb.3284 The crystal structure of HIV-1 accessory protein Vpr in complex with human UNG2 and DDB1-DCAF1 provides insight into how the viral protein directs UNG2 for degradation. Methyl transfer by substrate signaling from a knotted protein fold   pp941 - 948 Thomas Christian, Reiko Sakaguchi, Agata P Perlinska, Georges Lahoud, Takuhiro Ito et al. doi:10.1038/nsmb.3282 The structurally constrained knotted configuration of the RNA methyltransferase TrmD captures the free energy of substrate binding to facilitate catalysis. Top Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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