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A pulse of mid-Pleistocene rift volcanism in Ethiopia at the dawn of modern humans OPEN
William Hutchison, Raffaella Fusillo, David M. Pyle, Tamsin A. Mather, Jon D. Blundy, Juliet Biggs, Gezahegn Yirgu, Benjamin E. Cohen, Richard A. Brooker, Dan N. Barfod and Andrew T. Calvert
Past volcanic eruptions along the densely populated Ethiopian Rift valley remain poorly constrained despite the present day hazard. Hutchison et al. show that a large volcanic flare up along a 200 km section of the rift occurred between 320–170 ka dramatically affecting the landscape and hominin population.
18 October 2016 | doi: 10.1038/ncomms13192

Early cave art and ancient DNA record the origin of European bison OPEN
Julien Soubrier, Graham Gower, Kefei Chen, Stephen M. Richards, Bastien Llamas, Kieren J. Mitchell, Simon Y. W. Ho, Pavel Kosintsev, Michael S. Y. Lee, Gennady Baryshnikov, Ruth Bollongino, Pere Bover, Joachim Burger, David Chivall, Evelyne Crégut-Bonnoure, Jared E. Decker, Vladimir B. Doronichev, Katerina Douka, Damien A. Fordham, Federica Fontana et al.
The ancestry of the European bison (wisent) remains a mystery. Here, Cooper and colleagues examine ancient DNA from fossil remains of extinct bison, and reveal the wisent originated through the hybridization of the extinct Steppe bison and ancestors of modern cattle.
18 October 2016 | doi: 10.1038/ncomms13158

Structures and stabilization of kinetoplastid-specific split rRNAs revealed by comparing leishmanial and human ribosomes OPEN
Xing Zhang, Mason Lai, Winston Chang, Iris Yu, Ke Ding, Jan Mrazek, Hwee L. Ng, Otto O. Yang, Dmitri A. Maslov and Z. Hong Zhou
Leishmania donovani is a protozoan parasite that can cause fatal infections in humans. Here the authors present a high resolution cryoEM structure of the L. donovani 80S ribosome and compare it to its human counterpart to provide insight into the basis for drug selectivity towards this eukaryotic parasite.
18 October 2016 | doi: 10.1038/ncomms13223

Engineering prokaryotic channels for control of mammalian tissue excitability OPEN
Hung X. Nguyen, Robert D. Kirkton and Nenad Bursac
Restoring lost excitability of injured tissue is a paramount of regenerative medicine. By using a combined expression of bacterial voltage-gated Na+ channel, Kir2.1, and connexin-43 in non-excitable human fibroblasts, here the authors generate excitable cells that rescue action potential conduction in an in vitro model of cardiac fibrosis.
18 October 2016 | doi: 10.1038/ncomms13132

Ultrasmall and phase-pure W2C nanoparticles for efficient electrocatalytic and photoelectrochemical hydrogen evolution OPEN
Qiufang Gong, Yu Wang, Qi Hu, Jigang Zhou, Renfei Feng, Paul N. Duchesne, Peng Zhang, Fengjiao Chen, Na Han, Yafei Li, Chuanhong Jin, Yanguang Li and Shuit-Tong Lee
Tungsten carbide has yet to live up to its long-believed potential as a replacement for precious metal electrocatalysts. Here, Li and co-workers demonstrate that ditungsten carbide in the form of ultrasmall, phase-pure nanoparticles is a better candidate for the hydrogen evolution reaction.
18 October 2016 | doi: 10.1038/ncomms13216

Nicotinic acetylcholine receptor agonist attenuates ILC2-dependent airway hyperreactivity OPEN
Lauriane Galle-Treger, Yuzo Suzuki, Nisheel Patel, Ishwarya Sankaranarayanan, Jennifer L. Aron, Hadi Maazi, Lin Chen and Omid Akbari
Airway hyperreactivity is driven by type 2 cytokines produced by ILC2 and Th2 cells. Here the authors show that an α7-nicotinic receptor agonist (GTS-21) inhibits ILC2 responses and is therapeutic against Alternaria-induced airway hyperreactivity in a humanized mouse model.
18 October 2016 | doi: 10.1038/ncomms13202

The initiation of segmented buoyancy-driven melting during continental breakup OPEN
Ryan J. Gallacher, Derek Keir, Nicholas Harmon, Graham Stuart, Sylvie Leroy, James O. S. Hammond, J-Michael Kendall, Atalay Ayele, Berhe Goitom, Ghebrebrhan Ogubazghi and Abdulhakim Ahmed
Our understanding of melt production during early continental breakup remains poorly constrained. Using the Afar triple junction as an example, Gallacher et al. generate a 3D velocity model suggesting that melt production is highest during early continental breakup due to localised thinning of the crust.
18 October 2016 | doi: 10.1038/ncomms13110

Stereotypic wheel running decreases cortical activity in mice OPEN
Simon P. Fisher, Nanyi Cui, Laura E. McKillop, Jessica Gemignani, David M. Bannerman, Peter L. Oliver, Stuart N. Peirson and Vladyslav V. Vyazovskiy
Sleep need is thought to accumulate gradually over waking periods and is associated with changes in neuronal activity. Here the authors show that in mice cortical firing rates increase between the beginning and end of wakefulness periods but this increase is not seen in waking periods with voluntary stereotypic wheel running.
17 October 2016 | doi: 10.1038/ncomms13138

Human genome-wide RNAi screen reveals host factors required for enterovirus 71 replication OPEN
Kan Xing Wu, Patchara Phuektes, Pankaj Kumar, Germaine Yen Lin Goh, Dimitri Moreau, Vincent Tak Kwong Chow, Frederic Bard and Justin Jang Hann Chu
Enterovirus 71 (EV71) infection causes a spectrum of symptoms including neurological disease. To improve our understanding of EV71-host interactions, Wu et al. here perform a genome-wide RNAi screen, which implicates cell cycle regulation and ER-associated degradation as important factors in EV71 replication.
17 October 2016 | doi: 10.1038/ncomms13150

The reaction of methyl peroxy and hydroxyl radicals as a major source of atmospheric methanol OPEN
Jean-François Müller, Zhen Liu, Vinh Son Nguyen, Trissevgeni Stavrakou, Jeremy N. Harvey and Jozef Peeters
The high observed abundance of atmospheric methanol over remote oceans is still not well-explained. Here the authors use quantum calculations and atmospheric modelling to show the reaction of methyl peroxy and hydroxyl radicals is a major methanol source (115 Tg/yr), comparable to global terrestrial emissions.
17 October 2016 | doi: 10.1038/ncomms13213

Ordered fragmentation of oxide thin films at submicron scale OPEN
L. Guo, Y. Ren, L. Y. Kong, W. K. Chim and S. Y. Chiam
Fracture and related processes are typically considered detrimental, but have also attracted interest in more constructive roles. Here authors demonstrate ordered fragmentation at submicron scales of a metal oxide/hydroxide thin film by introducing preferential sites for fracture on the underlying substrate.
17 October 2016 | doi: 10.1038/ncomms13148

In vivo continuous evolution of genes and pathways in yeast OPEN
Nathan Crook, Joseph Abatemarco, Jie Sun, James M. Wagner, Alexander Schmitz and Hal S. Alper
Directed evolution is a powerful technique for generating improved biological systems through repeated rounds of mutagenesis and selection. Here the authors engineer the yeast retrotransposon Ty1 to enable the creation of large mutant libraries in vivo and use this system to generate improved variants of single enzymes and multigene pathways.
17 October 2016 | doi: 10.1038/ncomms13051

Scattering-type scanning near-field optical microscopy with low-repetition-rate pulsed light source through phase-domain sampling OPEN
Haomin Wang, Le Wang and Xiaoji G. Xu
Low repetition rate lasers are suitable for studying nonlinear optical phenomena, while near-field microscopy allows high spatial resolution for nanomaterial characterisation. Here, Wang et al. enable scattering-type near-field microscopy with low repetition rate lasers through phase-domain sampling.
17 October 2016 | doi: 10.1038/ncomms13212

Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1 OPEN
Bumpei Samata, Daisuke Doi, Kaneyasu Nishimura, Tetsuhiro Kikuchi, Akira Watanabe, Yoshimasa Sakamoto, Jungo Kakuta, Yuichi Ono and Jun Takahashi
Midbrain dopaminergic neurons generated from stem cells show promise for the treatment of Parkinson’s disease. Here, the authors use the cell surface marker, LRTM1, to enrich the midbrain dopaminergic progenitors and show improved motor function/cell survival when grafted into rat/monkey brains, respectively.
14 October 2016 | doi: 10.1038/ncomms13097

Boundaries steer the contraction of active gels OPEN
Matthias Schuppler, Felix C. Keber, Martin Kröger and Andreas R. Bausch
The actomyosin cytoskeleton consists of a contractile array but how it becomes organized is not clear. Here the authors reconstitute a controllable contractile system to show that force balances at boundaries determine contraction dynamics, and spatial anisotropy leads to self-organization or aligned contractile fibres.
14 October 2016 | doi: 10.1038/ncomms13120

CaMKII induces permeability transition through Drp1 phosphorylation during chronic β-AR stimulation OPEN
Shangcheng Xu, Pei Wang, Huiliang Zhang, Guohua Gong, Nicolas Gutierrez Cortes, Weizhong Zhu, Yisang Yoon, Rong Tian and Wang Wang
β-adrenergic receptor signaling induces mitochondrial permeability transition pore (mPTP) opening. Here, Xu et al. show that this effect is mediated by phosphorylation of mitochondrial fission protein Drp1 by CamKII, which increases the frequency of transient mPTP opening.
14 October 2016 | doi: 10.1038/ncomms13189

Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock OPEN
Alexandre Bagnoud, Karuna Chourey, Robert L. Hettich, Ino de Bruijn, Anders F. Andersson, Olivier X. Leupin, Bernhard Schwyn and Rizlan Bernier-Latmani
Hydrogen build-up in geological nuclear waste repositories poses risks, but it may be alleviated by H2 consumption by deep subsurface microbial communities. Here, the authors inject H2 in a borehole and use metagenomics and metaproteomics to identify a carbon cycle driven by autotrophic H2 oxidizers.
14 October 2016 | doi: 10.1038/ncomms12770

Glucocorticoid regulation of ATP release from spinal astrocytes underlies diurnal exacerbation of neuropathic mechanical allodynia OPEN
Satoru Koyanagi, Naoki Kusunose, Marie Taniguchi, Takahiro Akamine, Yuki Kanado, Yui Ozono, Takahiro Masuda, Yuta Kohro, Naoya Matsunaga, Makoto Tsuda, Michael W. Salter, Kazuhide Inoue and Shigehiro Ohdo
Neuropathic pain hypersensitivity is known to undergo diurnal variations, although the underlying mechanisms are not clear. Using a sciatic nerve-injury mouse model, the authors find such diurnal changes are mediated by glucocorticoid induced enhancement of ATP release from astrocytes via pannexin-1 hemichannels.
14 October 2016 | doi: 10.1038/ncomms13102

Massive and parallel expression profiling using microarrayed single-cell sequencing OPEN
Sanja Vickovic, Patrik L. Ståhl, Fredrik Salmén, Sarantis Giatrellis, Jakub Orzechowski Westholm, Annelie Mollbrink, José Fernández Navarro, Joaquin Custodio, Magda Bienko, Lesley-Ann Sutton, Richard Rosenquist, Jonas Frisén and Joakim Lundeberg
Currently available single-cell transcriptomic analyses are expensive and low throughput. Here, Vickovic et al. describe a new method called MASC-seq that is based on microarray barcoding of expression pattern and of low cost with high robustness.
14 October 2016 | doi: 10.1038/ncomms13182

Spin-polarized surface resonances accompanying topological surface state formation OPEN
Chris Jozwiak, Jonathan A. Sobota, Kenneth Gotlieb, Alexander F. Kemper, Costel R. Rotundu, Robert J. Birgeneau, Zahid Hussain, Dung-Hai Lee, Zhi-Xun Shen and Alessandra Lanzara
The spin-orbit interaction is central to the defining characteristics of topological insulators. Here, Jozwiak et al. report a spin-polarized unoccupied surface resonance coevolving with topological surface states from a pair of Rashba-like states through spin-orbit induced band inversion.
14 October 2016 | doi: 10.1038/ncomms13143

Simple phosphinate ligands access zinc clusters identified in the synthesis of zinc oxide nanoparticles OPEN
Sebastian D. Pike, Edward R. White, Milo S. P. Shaffer and Charlotte K. Williams
Ligands and surfactants play an important part in the synthesis of nanoparticles from molecular precursors although their exact roles are poorly understood. Here, the authors isolate a range of intermediate sized zinc clusters and are able to spectroscopically probe the self-assembly and ligand effects.
13 October 2016 | doi: 10.1038/ncomms13008

Macrophage-derived extracellular vesicle-packaged WNTs rescue intestinal stem cells and enhance survival after radiation injury OPEN
Subhrajit Saha, Evelyn Aranda, Yoku Hayakawa, Payel Bhanja, Safinur Atay, N Patrik Brodin, Jiufeng Li, Samuel Asfaha, Laibin Liu, Yagnesh Tailor, Jinghang Zhang, Andrew K. Godwin, Wolfgang A. Tome, Timothy C. Wang, Chandan Guha and Jeffrey W. Pollard
The intestinal stroma secretes WNT ligands but the role of WNT in intestinal repair is unclear. Here, the authors show that when WNT synthesis is ablated from stromal macrophages, the intestine morphology is normal but hypersensitive to radiation injury, implicating macrophage-derived WNT in intestinal repair.
13 October 2016 | doi: 10.1038/ncomms13096

Chiral optical response of planar and symmetric nanotrimers enabled by heteromaterial selection OPEN
Peter Banzer, Paweł Woźniak, Uwe Mick, Israel De Leon and Robert W. Boyd
Alternative ways to fabricate chiral media which give rise to interesting optical phenomena are sought. Here, Banzer et al. demonstrate a two-dimensional geometrically achiral nanoparticle assembly, which exhibits a chiral optical response due to its heterogeneous composition.
13 October 2016 | doi: 10.1038/ncomms13117

Flexible ferroelectric organic crystals OPEN
Magdalena Owczarek, Karl A. Hujsak, Daniel P. Ferris, Aleksandrs Prokofjevs, Irena Majerz, Przemysław Szklarz, Huacheng Zhang, Amy A. Sarjeant, Charlotte L. Stern, Ryszard Jakubas, Seungbum Hong, Vinayak P. Dravid and J. Fraser Stoddart
Mechanical flexibility gives organic ferroelectric materials a potential advantage over their inorganic counterparts, yet it is challenging to produce them. Owczarek et al. report flexible crystals based on trisubstituted haloimidazoles that show both ferroelectric and piezoelectric properties.
13 October 2016 | doi: 10.1038/ncomms13108

Structure-mechanism-based engineering of chemical regulators targeting distinct pathological factors in Alzheimer’s disease OPEN
Michael W. Beck, Jeffrey S. Derrick, Richard A. Kerr, Shin Bi Oh, Woo Jong Cho, Shin Jung C. Lee, Yonghwan Ji, Jiyeon Han, Zahra Aliakbar Tehrani, Nayoung Suh, Sujeong Kim, Scott D. Larsen, Kwang S. Kim, Joo-Yong Lee, Brandon T. Ruotolo and Mi Hee Lim
To advance our understanding of pathological features associated with Alzheimer’s disease (AD), chemical tools with distinct specificity towards AD targets would be valuable. Here the authors used a structure-mechanism-based design strategy to obtain small molecules as chemical regulators for distinct pathological factors linked to AD pathology.
13 October 2016 | doi: 10.1038/ncomms13115

LWD–TCP complex activates the morning gene CCA1 in Arabidopsis OPEN
Jing-Fen Wu, Huang-Lung Tsai, Ignasius Joanito, Yi-Chen Wu, Chin-Wen Chang, Yi-Hang Li, Ying Wang, Jong Chan Hong, Jhih-Wei Chu, Chao-Ping Hsu and Shu-Hsing Wu
The Arabidopsis CCA1 transcription factor is a core regulator of the circadian clock. Here, the authors show that the LWD1 protein, in complex with the TCP20 or TCP22 transcription factors, acts as a co-activator of CCA1 expression contributing to elevated CCA1 expression at dawn.
13 October 2016 | doi: 10.1038/ncomms13181

NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis OPEN
Dawei Yan, Vanathy Easwaran, Vivian Chau, Masanori Okamoto, Matthew Ierullo, Mitsuhiro Kimura, Akira Endo, Ryoichi Yano, Asher Pasha, Yunchen Gong, Yong-Mei Bi, Nicolas Provart, David Guttman, Anne Krapp, Steven J. Rothstein and Eiji Nambara
Nitrate stimulates seed germination in many plant species. Here, Yan et al. show that the Arabidopsis transcription factor NIN-like protein 8 is required to stimulate germination in response to nitrate and induces expression of an enzyme involved in ABA catabolism.
12 October 2016 | doi: 10.1038/ncomms13179

KCNE1 induces fenestration in the Kv7.1/KCNE1 channel complex that allows for highly specific pharmacological targeting OPEN
Eva Wrobel, Ina Rothenberg, Christoph Krisp, Franziska Hundt, Benjamin Fraenzel, Karina Eckey, Joannes T. M. Linders, David J. Gallacher, Rob Towart, Lutz Pott, Michael Pusch, Tao Yang, Dan M. Roden, Harley T. Kurata, Eric Schulze-Bahr, Nathalie Strutz-Seebohm, Dirk Wolters and Guiscard Seebohm
Specificity of inhibitors of voltage-gated ion channels is crucial for their use as therapeutics. Here, the authors show that adamantane derivatives interact with a specific binding site on fenestrations that only become available when accessory subunits are bound to the channel.
12 October 2016 | doi: 10.1038/ncomms12795

Polarization of M2 macrophages requires Lamtor1 that integrates cytokine and amino-acid signals OPEN
Tetsuya Kimura, Shigeyuki Nada, Noriko Takegahara, Tatsusada Okuno, Satoshi Nojima, Sujin Kang, Daisuke Ito, Keiko Morimoto, Takashi Hosokawa, Yoshitomo Hayama, Yuichi Mitsui, Natsuki Sakurai, Hana Sarashina-Kida, Masayuki Nishide, Yohei Maeda, Hyota Takamatsu, Daisuke Okuzaki, Masaki Yamada, Masato Okada and Atsushi Kumanogoh et al.
The role of nutrient-sensing pathways in regulation of innate immune response is unexplored. Here the authors show that IL-4 activates the amino-acid sensing pathway in macrophages and leads to polarization of anti-inflammatory M2 macrophages via the transcription factor liver X receptor.
12 October 2016 | doi: 10.1038/ncomms13130

Photo-excited charge carriers suppress sub-terahertz phonon mode in silicon at room temperature OPEN
Bolin Liao, A. A. Maznev, Keith A. Nelson and Gang Chen
Direct measurement of electron-phonon interactions at the single-mode level has been a challenge. Here, Liao et al. use a three-pulse photoacoustic spectroscopy technique to investigate the damping of a single sub-terahertz coherent phonon mode by photo-excited free charge carriers in silicon at room temperature.
12 October 2016 | doi: 10.1038/ncomms13174

The transcription factor Prox1 is essential for satellite cell differentiation and muscle fibre-type regulation OPEN
Riikka Kivelä, Ida Salmela, Yen Hoang Nguyen, Tatiana V. Petrova, Heikki A. Koistinen, Zoltan Wiener and Kari Alitalo
Skeletal muscle has remarkable adaptive and regenerative capacity. Here the authors show that the transcription factor Prox1 is necessary for maintenance of slow muscle fibre types via activation of NFAT signalling, and for myoblast differentiation via cross-talk with the Notch signalling pathway.
12 October 2016 | doi: 10.1038/ncomms13124

Nicotinamide is an endogenous agonist for a C. elegans TRPV OSM-9 and OCR-4 channel OPEN
Awani Upadhyay, Aditya Pisupati, Timothy Jegla, Matt Crook, Keith J. Mickolajczyk, Matthew Shorey, Laura E. Rohan, Katherine A. Billings, Melissa M. Rolls, William O. Hancock and Wendy Hanna-Rose
TRPV are cation channels activated by physical and chemical stimuli. Here the authors show that nicotinamide is a soluble, endogenous agonist for orthologous TRPV channels from C. elegans and Drosophila, unveiling a metabolic-based regulation for TRPV channel activity.
12 October 2016 | doi: 10.1038/ncomms13135

Self-assembly of acetate adsorbates drives atomic rearrangement on the Au(110) surface OPEN
Fanny Hiebel, Bonggeun Shong, Wei Chen, Robert J. Madix, Efthimios Kaxiras and Cynthia M. Friend
The efficiency of a catalyst relies on the stability of intermediates on its surface. Here, the authors find that van der Waals interactions between acetate adsorbates on Au(110) provide a small but necessary energy contribution to stabilize the acetate and drive restructuring of the Au surface.
12 October 2016 | doi: 10.1038/ncomms13139

Specification of haematopoietic stem cell fate via modulation of mitochondrial activity OPEN
Nicola Vannini, Mukul Girotra, Olaia Naveiras, Gennady Nikitin, Vasco Campos, Sonja Giger, Aline Roch, Johan Auwerx and Matthias P. Lutolf
Haematopoietic stem cells rely on glycolysis for their energy demands but whether this affects their fate is unknown. Here, the authors show that forcing the cells to rely on glycolysis is important for self-renewal and that this involves a reduction in mitochondrial mass.
12 October 2016 | doi: 10.1038/ncomms13125
 
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Editorial Expression of Concern: Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats OPEN
Sebastian Sjöqvist, Philipp Jungebluth, Mei Ling Lim, Johannes C. Haag, Ylva Gustafsson, Greg Lemon, Silvia Baiguera, Miguel Angel Burguillos, Costantino Del Gaudio, Antonio Beltran Rodriguez, Alexander Sotnichenko, Karolina Kublickiene, Henrik Ullman, Heike Kielstein, Peter Damberg, Alessandra Bianco, Rainer Heuchel, Ying Zhao, Domenico Ribatti, Cristián Ibarra et al.
14 October 2016 | doi: 10.1038/ncomms13310
 
 

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