Scientific Reports Biology Table of Contents e-alert: 20 September 2016

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20 September 2016      • Visit Scientific Reports • Submit your manuscript to Scientific Reports     Welcome to your Table of Contents e-alert for Scientific Reports       Biological Sciences               Potential pitfalls of mass spectrometry to uncover mutations in childhood soft tissue sarcoma: A report from the Children’s Oncology Group ▶     Lin Xu, Raphael A. Wilson, Theodore W. Laetsch et al.     Mass spectrometry-based methods have been widely applied – often as the sole method – to detect mutations in human cancer specimens. We applied this approach to 52 childhood soft …             Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption ▶     Elsa Mével, Christophe Merceron, Claire Vinatier et al.     Polyphenols exert a large range of beneficial effects in the prevention of age-related diseases. We sought to determine whether an extract of olive and grape seed standardized …             Synchrotron microbeam irradiation induces neutrophil infiltration, thrombocyte attachment and selective vascular damage in vivo ▶     Daniel Brönnimann, Audrey Bouchet, Christoph Schneider et al.     Our goal was the visualizing the vascular damage and acute inflammatory response to micro- and minibeam irradiation in vivo. Microbeam (MRT) and minibeam radiation therapies …             β-Hairpin of Islet Amyloid Polypeptide Bound to an Aggregation Inhibitor ▶     Ewa A. Mirecka, Sophie Feuerstein, Lothar Gremer et al.     In type 2 diabetes, the formation of islet amyloid consisting of islet amyloid polypeptide (IAPP) is associated with reduction in β-cell mass and contributes to the failure of …             Lactobacillus plantarum attenuates anxiety-related behavior and protects against stress-induced dysbiosis in adult zebrafish ▶     Daniel J. Davis, Holly M. Doerr, Agata K. Grzelak et al.     The consumption of probiotics has become increasingly popular as a means to try to improve health and well-being. Not only are probiotics considered beneficial to digestive …             Full-length model of the human galectin-4 and insights into dynamics of inter-domain communication ▶     Joane K. Rustiguel, Ricardo O. S. Soares, Steve P. Meisburger et al.     Galectins are proteins involved in diverse cellular contexts due to their capacity to decipher and respond to the information encoded by β-galactoside sugars. In particular, …             Thermal stress depletes energy reserves in Drosophila ▶     Peter Klepsatel, Martina Gáliková, Yanjun Xu et al.     Understanding how environmental temperature affects metabolic and physiological functions is of crucial importance to assess the impacts of climate change on organisms. Here, we …             A Direct Comparison of Two Densely Sampled HIV Epidemics: The UK and Switzerland ▶     Manon L. Ragonnet-Cronin, Mohaned Shilaih, Huldrych F. Günthard et al.     Phylogenetic clustering approaches can elucidate HIV transmission dynamics. Comparisons across countries are essential for evaluating public health policies. Here, we used a …             Complement component 3 deficiency prolongs MHC-II disparate skin allograft survival by increasing the CD4+ CD25+ regulatory T cells population ▶     Quan-you Zheng, Shen-ju Liang, Gui-qing Li et al.     Recent reports suggest that complement system contributes to allograft rejection. However, its underlying mechanism is poorly understood. Herein, we investigate the role of …             SCOP/PHLPP1β in the basolateral amygdala regulates circadian expression of mouse anxiety-like behavior ▶     Jun J. Nakano, Kimiko Shimizu, Shigeki Shimba et al.     While disruption of the circadian clock triggers a spectrum of affective abnormalities, how the clock regulates mammalian emotionality remains unclear. Here, we characterized the …             Nuclear localization of tricellulin promotes the oncogenic property of pancreatic cancer ▶     Akira Takasawa, Masaki Murata, Kumi Takasawa et al.     Accumulating evidence has shown that dysregulation of tight junctions (TJs) is involved in tumor development and progression. In this study, we investigated the expression and …             Real-time and label-free monitoring of nanoparticle cellular uptake using capacitance-based assays ▶     Rimi Lee, Dong hyun Jo, Sang J. Chung et al.     Nanoparticles have shown great potential as vehicles for the delivery of drugs, nucleic acids, and therapeutic proteins; an efficient, high-throughput screening method to analyze …             Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents ▶     Ana Gámez-Valero, Marta Monguió-Tortajada, Laura Carreras-Planella et al.     Extracellular vesicles (EVs) have become an attractive field among the scientific community. Yet, a major challenge is to define a consensus method for EVs isolation. Ul…             Turning the fate of reprogramming cells from retinal disorder to regeneration by Pax6 in newts ▶     Martin Miguel Casco-Robles, Md Rafiqul Islam, Wataru Inami et al.     The newt, a urodele amphibian, has an outstanding ability– even as an adult –to regenerate a functional retina through reprogramming and proliferation of the retinal pigment …             A simple and cost-saving phenotypic drug susceptibility testing of HIV-1 ▶     Yunceng Weng, Ling Zhang, Jianfeng Huang et al.     It is essential to monitor the occurrence of drug-resistant strains and to provide guidance for clinically adapted antiviral treatment of HIV/AIDS. In this study, an individual …             Ultra-sensitive detection of brain-derived neurotrophic factor (BDNF) in the brain of freely moving mice using an interdigitated microelectrode (IME) biosensor ▶     Yong Kyoung Yoo, Jaekwang Lee, Jinsik Kim et al.     Brain-derived neurotrophic factor (BDNF) plays a critical role in cognitive processes including learning and memory. However, it has been difficult to detect BDNF in the brains …             Macrophages facilitate the excystation and differentiation of Toxoplasma gondii sporozoites into tachyzoites following oocyst internalisation ▶     Wesley Freppel, Pierre-Henri Puech, David J. P. Ferguson et al.     Toxoplasma gondii is a common parasite of humans and animals, which is transmitted via oocysts in cat faeces or tissue cysts in contaminated meat. The robust oocyst and sporocyst …             Suture pattern formation in ammonites and the unknown rear mantle structure ▶     Shinya Inoue, Shigeru Kondo     Ammonite shells have complex patterns of suture lines that vary across species. The lines are formed at the intersection of the outer shell wall and the septa. The wavy septa can …             Improving methane production in cow dung and corn straw co-fermentation systems via enhanced degradation of cellulose by cabbage addition ▶     Wenyang Wu, Yong Chen, Shah Faisal et al.     The effects of cabbage waste (CW) addition on methane production in cow dung and corn straw co-fermentation systems were investigated. Four experimental groups, each containing …             Automatic Bayesian single molecule identification for localization microscopy ▶     Yunqing Tang, Johnny Hendriks, Thomas Gensch et al.     Single molecule localization microscopy (SMLM) is on its way to become a mainstream imaging technique in the life sciences. However, analysis of SMLM data is biased by user …             iRSpot-DACC: a computational predictor for recombination hot/cold spots identification based on dinucleotide-based auto-cross covariance ▶     Bingquan Liu, Yumeng Liu, Xiaopeng Jin et al.     Meiotic recombination presents an uneven distribution across the genome. Genomic regions that exhibit at relatively high frequencies of recombination are called hotspots, whereas …             Reactive oxygen species do not contribute to ObgE*-mediated programmed cell death ▶     Liselot Dewachter, Pauline Herpels, Natalie Verstraeten et al.     Programmed cell death (PCD) in bacteria is considered an important target for developing novel antimicrobials. Development of PCD-specific therapies requires a deeper …             Interoceptive Ability Predicts Survival on a London Trading Floor ▶     Narayanan Kandasamy, Sarah N. Garfinkel, Lionel Page et al.     Interoception is the sensing of physiological signals originating inside the body, such as hunger, pain and heart rate. People with greater sensitivity to interoceptive signals, …             Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects ▶     Laurence Borgs, Elise Peyre, Philippe Alix et al.     Some mutations of the LRRK2 gene underlie autosomal dominant form of Parkinson’s disease (PD). The G2019S is a common mutation that accounts for about 2% of PD cases. To …             BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer’s disease models ▶     Hisae Nishioka, Norie Tooi, Takehisa Isobe et al.     Alzheimer’s disease (AD) is the most common form of dementia. Cellular AD models derived from human pluripotent stem cells are promising tools in AD research. We recently …             In Situ Anabolic Activity of Periodontal Pathogens Porphyromonas gingivalis and Filifactor alocis in Chronic Periodontitis ▶     Ralee Spooner, Kris M. Weigel, Peter L. Harrison et al.     Porphyromonas gingivalis and Filifactor alocis are fastidious anaerobic bacteria strongly associated with chronic forms of periodontitis. Our understanding of the growth …             Mutation analysis of circulating plasma DNA to determine response to EGFR tyrosine kinase inhibitor therapy of lung adenocarcinoma patients ▶     Anja Lisa Riediger, Steffen Dietz, Uwe Schirmer et al.     Long-lasting success in lung cancer therapy using tyrosine kinase inhibitors (TKIs) is rare since the tumors develop resistance due to the occurrence of molecularly altered …             The miR-33 gene is identified in a marine teleost: a potential role in regulation of LC-PUFA biosynthesis in Siganus canaliculatus ▶     Qinghao Zhang, Cuihong You, Shuqi Wang et al.     As the first marine teleost demonstrated to have the ability to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFA) from C18 PUFA precursors, rabbitfish Siganus …             Drought response transcriptomes are altered in poplar with reduced tonoplast sucrose transporter expression ▶     Liang-Jiao Xue, Christopher J. Frost, Chung-Jui Tsai et al.     Transgenic Populus tremula x alba (717-1B4) plants with reduced expression of a tonoplast sucrose efflux transporter, PtaSUT4, exhibit reduced shoot growth compared to wild type …             Assimilation of virtual legs and perception of floor texture by complete paraplegic patients receiving artificial tactile feedback ▶     Solaiman Shokur, Simone Gallo, Renan C. Moioli et al.     Spinal cord injuries disrupt bidirectional communication between the patient’s brain and body. Here, we demonstrate a new approach for reproducing lower limb somatosensory …             Quantitative and combinatory determination of in situ phosphorylation of tau and its FTDP-17 mutants ▶     Taeko Kimura, Tomohisa Hosokawa, Masato Taoka et al.     Tau is hyperphosphorylated in the brains of patients with tauopathies, such as Alzheimer’s disease and frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17). …             N-Glycosylation of integrin α5 acts as a switch for EGFR-mediated complex formation of integrin α5β1 to α6β4 ▶     Qinglei Hang, Tomoya Isaji, Sicong Hou et al.     N-Glycosylation of integrin α5β1 is involved in multiple cell behaviors. We previously reported that the N-glycosylations of the calf domain on integrin α5 (S3–5,10–14) are …             Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum ▶     Michael E. Pyne, Xuejia Liu, Murray Moo-Young et al.     Clostridium pasteurianum is emerging as a prospective host for the production of biofuels and chemicals, and has recently been shown to directly consume electric current. Despite …             Inhibition promotes long-term potentiation at cerebellar excitatory synapses ▶     F. Binda, K. Dorgans, S. Reibel et al.     The ability of the cerebellar cortex to learn from experience ensures the accuracy of movements and reflex adaptation, processes which require long-term plasticity at granule …             ATP from synaptic terminals and astrocytes regulates NMDA receptors and synaptic plasticity through PSD-95 multi-protein complex ▶     U. Lalo, O. Palygin, A. Verkhratsky et al.     Recent studies highlighted the importance of astrocyte-secreted molecules, such as ATP, for the slow modulation of synaptic transmission in central neurones. Biophysical …             Uncovering the lipidic basis for the preparation of functional nicotinic acetylcholine receptor detergent complexes for structural studies ▶     Orestes Quesada, Carol González -Freire, María Carla Ferrer et al.     This study compares the lipid composition, including individual phospholipid molecular species of solubilized nAChR detergent complexes (nAChR-DCs) with those of the bulk lipids …             DNA barcoding reveal patterns of species diversity among northwestern Pacific molluscs ▶     Shao’e Sun, Qi Li, Lingfeng Kong et al.     This study represents the first comprehensive molecular assessment of northwestern Pacific molluscs. In total, 2801 DNA barcodes belonging to 569 species from China, Japan and …             Papain gel containing methylene blue for simultaneous caries removal and antimicrobial photoinactivation against Streptococcus mutans biofilms ▶     Zenildo Santos Silva Jr., Ying-Ying Huang, Lucas Freitas de Freitas et al.     This study intended to evaluate the effects of a papain-gel with a red-light absorbing pigment (methylene blue – MB) to mediate photodynamic therapy (PDT) against Streptococcus …             Soil surface temperatures reveal moderation of the urban heat island effect by trees and shrubs ▶     J. L. Edmondson, I. Stott, Z. G. Davies et al.     Urban areas are major contributors to air pollution and climate change, causing impacts on human health that are amplified by the microclimatological effects of buildings and …             Characterization of the mechanism of prolonged adaptation to osmotic stress of Jeotgalibacillus malaysiensis via genome and transcriptome sequencing analyses ▶     Amira Suriaty Yaakop, Kok-Gan Chan, Robson Ee et al.     Jeotgalibacillus malaysiensis, a moderate halophilic bacterium isolated from a pelagic area, can endure higher concentrations of sodium chloride (NaCl) than other Jeotgalibacillus…             Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers ▶     Evelyn Ploetz, Eitan Lerner, Florence Husada et al.     Advanced microscopy methods allow obtaining information on (dynamic) conformational changes in biomolecules via measuring a single molecular distance in the structure. It is, …             Flexible Neural Electrode Array Based-on Porous Graphene for Cortical Microstimulation and Sensing ▶     Yichen Lu, Hongming Lyu, Andrew G. Richardson et al.     Neural sensing and stimulation have been the backbone of neuroscience research, brain-machine interfaces and clinical neuromodulation therapies for decades. To-date, most of the …             Structural Analysis of the Hg(II)-Regulatory Protein Tn501 MerR from Pseudomonas aeruginosa ▶     Dan Wang, Shanqing Huang, Pingying Liu et al.     The metalloprotein MerR is a mercury(II)-dependent transcriptional repressor-activator that responds to mercury(II) with extraordinary sensitivity and selectivity. It’s widely …             Dissemination of IMP-4-encoding pIMP-HZ1-related plasmids among Klebsiella pneumoniae and Pseudomonas aeruginosa in a Chinese teaching hospital ▶     Wei Feng, Dongsheng Zhou, Qian Wang et al.     A total of 26 blaIMP-4-carrying strains of Pseudomonas aeruginosa and Klebsiella pneumoniae were isolated from 2009 to 2013 in a Chinese teaching hospital, and these strains can …             Circulation of multiple serotypes of highly divergent enterovirus C in the Xinjiang Uighur Autonomous Region of China ▶     Yong Zhang, Qiang Sun, Hui Cui et al.     Poliomyelitis associated with circulating vaccine-derived polioviruses (cVDPVs) is a serious public health issue in the post-eradication era, and the occurrence of recombinant …             Lack of adrenomedullin in mouse endothelial cells results in defective angiogenesis, enhanced vascular permeability, less metastasis, and more brain damage ▶     Laura Ochoa-Callejero, Andrea Pozo-Rodrigálvarez, Ricardo Martínez-Murillo et al.     Adrenomedullin (AM) is a vasodilating peptide involved in the regulation of circulatory homeostasis and in the pathophysiology of certain cardiovascular diseases. AM plays …             Hybrid bright-field and hologram imaging of cell dynamics ▶     Hyeokjun Byeon, Jaehyun Lee, Junsang Doh et al.     Volumetric observation is essential for understanding the details of complex biological phenomena. In this study, a bright-field microscope, which provides information on a …             Circulating tumor DNA detection in lung cancer patients before and after surgery ▶     Nannan Guo, Feng Lou, Yongfu Ma et al.     Circulating tumor DNA (ctDNA) in peripheral blood is a “liquid biopsy” that contains representative tumor information including gene mutations. Additionally, repeated ctDNA …             Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes ▶     Michele Munk, Luiz O. Ladeira, Bruno C. Carvalho et al.     The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for …             Global analysis of transcription in castration-resistant prostate cancer cells uncovers active enhancers and direct androgen receptor targets ▶     Sari Toropainen, Einari A. Niskanen, Marjo Malinen et al.     Androgen receptor (AR) is a male sex steroid-activated transcription factor (TF) that plays a critical role in prostate cancers, including castration-resistant prostate cancers …             TSPO ligands stimulate ZnPPIX transport and ROS accumulation leading to the inhibition of P. falciparum growth in human blood ▶     I. Marginedas-Freixa, C. Hattab, G. Bouyer et al.     After invading red blood cells (RBCs), Plasmodium falciparum (Pf) can export its own proteins to the host membrane and activate endogenous channels that are present in the …             Exploring the transcriptome of Staphylococcus aureus in its natural niche ▶     Diego Chaves-Moreno, Melissa L. Wos-Oxley, Ruy Jáuregui et al.     Staphylococcus aureus is an important human pathogen and commensal, where the human nose is the predominant reservoir. To better understand its behavior in this environmental …             Single-Molecule Imaging Reveals the Activation Dynamics of Intracellular Protein Smad3 on Cell Membrane ▶     Nan Li, Yong Yang, Kangmin He et al.     Smad3 is an intracellular protein that plays a key role in propagating transforming growth factor β (TGF-β) signals from cell membrane to nucleus. However whether the transient …             IL-6, IL-10 and TNFα do not improve early detection of post-endoscopic retrograde cholangiopancreatography acute pancreatitis: a prospective cohort study ▶     Mar Concepción-Martín, Cristina Gómez-Oliva, Ana Juanes et al.     The most reliable indicators for post-ERCP acute pancreatitis are elevated amylase levels and abdominal pain 24 hours after ERCP. As ERCP is often performed on an outpatient …             Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats ▶     Shukui Yu, Shenglian Yao, Yujun Wen et al.     This study examined sustained co-delivery of vascular endothelial growth factor (VEGF), angiopoietin-1 and basic fibroblast growth factor (bFGF) encapsulated in angiogenic …             Functional Recovery of AQP2 Recessive Mutations Through Hetero-Oligomerization with Wild-Type Counterpart ▶     Abdulah El Tarazi, Yoann Lussier, Sandra Da Cal et al.     Aquaporin-2 (AQP2) is a homotetrameric water channel responsible for the final water reuptake in the kidney. Mutations in the protein induce nephrogenic diabetes insipidus (NDI), …             Linked color imaging application for improving the endoscopic diagnosis accuracy: a pilot study ▶     Xiaotian Sun, Tenghui Dong, Yiliang Bi et al.     Endoscopy has been widely used in diagnosing gastrointestinal mucosal lesions. However, there are still lack of objective endoscopic criteria. Linked color imaging (LCI) is newly …             Evaluation of Glutathione Peroxidase 4 role in Preeclampsia ▶     Xinguo Peng, Yan Lin, Jinling Li et al.     Preeclampsia (PE) is a pregnancy-specific syndrome that may be lifethreatening to pregnancies and fetus. Glutathione Peroxidase 4 (GPx4) is a powerful antioxidant enzyme that can …             Transient receptor potential channel 1/4 reduces subarachnoid hemorrhage-induced early brain injury in rats via calcineurin-mediated NMDAR and NFAT dephosphorylation ▶     Zhong Wang, Yibin Wang, Xiaodi Tian et al.     Transient receptor potential channel 1/4 (TRPC1/4) are considered to be related to subarachnoid hemorrhage (SAH)-induced cerebral vasospasm. In this study, a SAH rat model was …             Combining Exosomes Derived from Immature DCs with Donor Antigen-Specific Treg Cells Induces Tolerance in a Rat Liver Allograft Model ▶     Ben Ma, Jing-Yue Yang, Wen-jie Song et al.     Allograft tolerance is the ultimate goal in the field of transplantation immunology. Immature dendritic cells (imDCs) play an important role in establishing tolerance but have …             Nox2 contributes to the arterial endothelial specification of mouse induced pluripotent stem cells by upregulating Notch signaling ▶     Xueling Kang, Xiangxiang Wei, Xinhong Wang et al.     Reactive oxygen species (ROS) have a crucial role in stem-cell differentiation; however, the mechanisms by which ROS regulate the differentiation of stem cells into endothelial …             A combination hepatoma-targeted therapy based on nanotechnology: pHRE-Egr1-HSV-TK/131I-antiAFPMcAb-GCV/MFH ▶     Mei Lin, Junxing Huang, Xingmao Jiang et al.     Combination targeted therapy is a promising cancer therapeutic strategy. Here, using PEI-Mn0.5Zn0.5Fe2O4 nanoparticles (PEI-MZF-NPs) as magnetic media for MFH (magnetic fluid …             Human CB1 Receptor Isoforms, present in Hepatocytes and β-cells, are Involved in Regulating Metabolism ▶     Isabel González-Mariscal, Susan M. Krzysik-Walker, Máire E. Doyle et al.     Therapeutics aimed at blocking the cannabinoid 1 (CB1) receptor for treatment of obesity resulted in significant improvements in liver function, glucose uptake and pancreatic …             The N-terminal domain plays a crucial role in the structure of a full-length human mitochondrial Lon protease ▶     Sami Kereïche, Lubomír Kováčik, Jan Bednár et al.     Lon is an essential, multitasking AAA+ protease regulating many cellular processes in species across all kingdoms of life. Altered expression levels of the human mitochondrial …             Orange protein has a role in phytoene synthase stabilization in sweetpotato ▶     Seyeon Park, Ho Soo Kim, Young Jun Jung et al.     Carotenoids have essential roles in light-harvesting processes and protecting the photosynthetic machinery from photo-oxidative damage. Phytoene synthase (PSY) and Orange (Or) …             Identification of Doxorubicin as an Inhibitor of the IRE1α-XBP1 Axis of the Unfolded Protein Response ▶     Dadi Jiang, Connor Lynch, Bruno C. Medeiros et al.     Activation of the IRE1α-XBP1 branch of the unfolded protein response (UPR) has been implicated in multiple types of human cancers, including multiple myeloma (MM). Through an in …             Modification of Susceptible and Toxic Herbs on Grassland Disease ▶     Xiang Yao, Yubing Fan, Qing Chai et al.     Recent research shows that continuous overgrazing not only causes grassland biodiversity to decline, but also causes light fungal disease. Achnatherum inebrians is susceptible to …             Maternal Genetic Ancestry and Legacy of 10th Century AD Hungarians ▶     Aranka Csősz, Anna Szécsényi-Nagy, Veronika Csákyová et al.     The ancient Hungarians originated from the Ural region in today’s central Russia and migrated across the Eastern European steppe, according to historical sources. The Hungarians …             The Tara Oceans voyage reveals global diversity and distribution patterns of marine planktonic ciliates ▶     Anna Gimmler, Ralf Korn, Colomban de Vargas et al.     Illumina reads of the SSU-rDNA-V9 region obtained from the circumglobal Tara Oceans expedition allow the investigation of protistan plankton diversity patterns on a global scale. …             ZikaVR: An Integrated Zika Virus Resource for Genomics, Proteomics, Phylogenetic and Therapeutic Analysis ▶     Amit Kumar Gupta, Karambir Kaur, Akanksha Rajput et al.     Current Zika virus (ZIKV) outbreaks that spread in several areas of Africa, Southeast Asia, and in pacific islands is declared as a global health emergency by World Health …             Micro-optical coherence tomography of the mammalian cochlea ▶     Janani S. Iyer, Shelley A. Batts, Kengyeh K. Chu et al.     The mammalian cochlea has historically resisted attempts at high-resolution, non-invasive imaging due to its small size, complex three-dimensional structure, and embedded …             Enhanced mechanical performance of biocompatible hemicelluloses-based hydrogel via chain extension ▶     Xian-Ming Qi, Ge-Gu Chen, Xiao-Dong Gong et al.     Hemicelluloses are widely used to prepare gel materials because of their renewability, biodegradability, and biocompatibility. Here, molecular chain extension of hemicelluloses …             USP16 Downregulation by Carboxyl-terminal Truncated HBx Promotes the Growth of Hepatocellular Carcinoma Cells ▶     Yu Qian, Boshi Wang, Aihui Ma et al.     Hepatitis B virus (HBV) infection is a major factor that contributes to the development of hepatocellular carcinoma (HCC). HBV X protein (HBx) has been shown to accelerate HCC …             A highly efficient sorbitol dehydrogenase from Gluconobacter oxydans G624 and improvement of its stability through immobilization ▶     Tae-Su Kim, Sanjay K. S. Patel, Chandrabose Selvaraj et al.     A sorbitol dehydrogenase (GoSLDH) from Gluconobacter oxydans G624 (G. oxydans G624) was expressed in Escherichia coli BL21(DE3)-CodonPlus RIL. The complete 1455-bp …             Discovery and Optimization of N-Substituted 2-(4-pyridinyl)thiazole carboxamides against Tumor Growth through Regulating Angiogenesis Signaling Pathways ▶     Wenbo Zhou, Wenshu Tang, Zhenliang Sun et al.     Inhibition of angiogenesis is considered as one of the desirable pathways for the treatment of tumor growth and metastasis. Herein we demonstrated that a series of …             Horizontal gene transfer drives adaptive colonization of apple trees by the fungal pathogen Valsa mali ▶     Zhiyuan Yin, Baitao Zhu, Hao Feng et al.     Horizontal gene transfer (HGT) often has strong benefits for fungi. In a study of samples from apple canker in Shaanxi Province, China, diverse microbes, along with the …             Improved Transgenic Mouse Model for Studying HLA Class I Antigen Presentation ▶     Man Huang, Wei Zhang, Jie Guo et al.     HLA class I (HLA-I) transgenic mice have proven to be useful models for studying human MHC-related immune responses over the last two decades. However, differences in the …             Determination by near infrared microscopy of the nitrogen and carbon content of tomato (Solanum lycopersicum L.) leaf powder ▶     Gauthier Lequeue, Xavier Draye, Vincent Baeten     Near infrared microscopy (NIRM) has been developed as a rapid technique to predict the chemical composition of foods, reduce analytical costs and time and ease sample …             Corrigendum: Environmental chemicals impact dog semen quality in vitro and may be associated with a temporal decline in sperm motility and increased cryptorchidism ▶     Richard G. Lea, Andrew S. Byers, Rebecca N. Sumner et al.                 Rare variants in BRCA2 and CHEK2 are associated with the risk of urinary tract cancers ▶     Yuqiu Ge, Yunyan Wang, Wei Shao et al.     Previous studies have shown that two rare variants, rs11571833 in BRCA2 and rs17879961 in CHEK2 were associated with lung cancer. However, the associations between these two …             THBS2 is a Potential Prognostic Biomarker in Colorectal Cancer ▶     Xue Wang, Lei Zhang, Hui Li et al.     Colorectal cancer is one of the most common leading causes of death worldwide. Prognostic at an early stage is a useful way that decrease and avoid mortality. Although remarkable …             Extracellular cGMP Modulates Learning Biphasically by Modulating Glycine Receptors, CaMKII and Glutamate-Nitric Oxide-cGMP Pathway ▶     Andrea Cabrera-Pastor, Michele Malaguarnera, Lucas Taoro-Gonzalez et al.     It has been proposed that extracellular cGMP modulates the ability to learn a Y maze task, but the underlying mechanisms remained unknown. Here we show that extracellular cGMP, …             Towards Biocontained Cell Factories: An Evolutionarily Adapted Escherichia coli Strain Produces a New-to-nature Bioactive Lantibiotic Containing Thienopyrrole-Alanine ▶     Anja Kuthning, Patrick Durkin, Stefan Oehm et al.     Genetic code engineering that enables reassignment of genetic codons to non-canonical amino acids (ncAAs) is a powerful strategy for enhancing ribosomally synthesized peptides …             Chromatin remodeling during the in vivo glial differentiation in early Drosophila embryos ▶     Youqiong Ye, Liang Gu, Xiaolong Chen et al.     Chromatin remodeling plays a critical role in gene regulation and impacts many biological processes. However, little is known about the relationship between chromatin remodeling …             Phenotypic, histological and proteomic analyses reveal multiple differences associated with chloroplast development in yellow and variegated variants from Camellia sinensis ▶     Chengying Ma, Junxi Cao, Jianke Li et al.     Leaf colour variation is observed in several plants. We obtained two types of branches with yellow and variegated leaves from Camellia sinensis. To reveal the mechanisms that …             Integrated stress response of vertebrates is regulated by four eIF2α kinases ▶     Shusuke Taniuchi, Masato Miyake, Kazue Tsugawa et al.     The integrated stress response (ISR) is a cytoprotective pathway initiated upon phosphorylation of the eukaryotic translation initiation factor 2 (eIF2α) residue designated …             Characterisation of a flavonoid ligand of the fungal protein Alt a 1 ▶     María Garrido-Arandia, Javier Silva-Navas, Carmen Ramírez-Castillejo et al.     Spores of pathogenic fungi are virtually ubiquitous and cause human disease and severe losses in crops. The endophytic fungi Alternaria species produce host-selective …             An integrative and comparative study of pan-cancer transcriptomes reveals distinct cancer common and specific signatures ▶     Zhen Cao, Shihua Zhang     To investigate the commonalities and specificities across tumor lineages, we perform a systematic pan-cancer transcriptomic study across 6744 specimens. We find six pan-cancer …             Investigation of rare and low-frequency variants using high-throughput sequencing with pooled DNA samples ▶     Jingwen Wang, Tiina Skoog, Elisabet Einarsdottir et al.     High-throughput sequencing using pooled DNA samples can facilitate genome-wide studies on rare and low-frequency variants in a large population. Some major questions concerning …             Regulation of osteoblast development by Bcl-2-associated athanogene-1 (BAG-1) ▶     Joanna Greenhough, Emmanouil S. Papadakis, Ramsey I. Cutress et al.     BCL-2-associated athanogene-1 (BAG-1) is expressed by osteoblast-lineage cells; early embryonic lethality in Bag-1 null mice, however, has limited the investigation of BAG-1 …             Protein Folding Activity of the Ribosome is involved in Yeast Prion Propagation ▶     Marc Blondel, Flavie Soubigou, Justine Evrard et al.     6AP and GA are potent inhibitors of yeast and mammalian prions and also specific inhibitors of PFAR, the protein-folding activity borne by domain V of the large rRNA of the large …             Nonlethal predator effects on the turn-over of wild bird flocks ▶     Bernhard Voelkl, Josh A. Firth, Ben C. Sheldon     Nonlethal predator effects arise when individuals of a prey species adjust their behaviour due to the presence of predators. Non-lethal predator effects have been shown to affect …             Comparison of research methods for functional characterization of insect olfactory receptors ▶     Bing Wang, Yang Liu, Kang He et al.     Insect olfactory receptors (ORs) in the peripheral olfactory system play an important role detecting elements of information from the environment. At present, various approaches …             Corrigendum: Polymorphisms in the Mannose-Binding Lectin Gene are Associated with Defective Mannose-Binding Lectin Functional Activity in Crohn’s Disease Patients ▶     Laura Choteau, Francis Vasseur, Frederic Lepretre et al.                 Protein aggregation activates erratic stress response in dietary restricted yeast cells ▶     Ankan Kumar Bhadra, Eshita Das, Ipsita Roy     Chronic stress and prolonged activation of defence pathways have deleterious consequences for the cell. Dietary restriction is believed to be beneficial as it induces the …             TGF-β1 autocrine signalling and enamel matrix components ▶     Saeko Kobayashi-Kinoshita, Yasuo Yamakoshi, Kazuo Onuma et al.     Transforming growth factor-β1 (TGF-β1) is present in porcine enamel extracts and is critical for proper mineralization of tooth enamel. Here, we show that the mRNA of latent …             Redox-dependent Regulation of Gluconeogenesis by a Novel Mechanism Mediated by a Peroxidatic Cysteine of Peroxiredoxin ▶     Hayato Irokawa, Tsuyoshi Tachibana, Toshihiko Watanabe et al.     Peroxiredoxin is an abundant peroxidase, but its non-peroxidase function is also important. In this study, we discovered that Tsa1, a major peroxiredoxin of budding yeast cells, …             Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility ▶     I. M. Aparicio, J. Espino, I. Bejarano et al.     Macroautophagy (hereafter autophagy) is an evolutionarily highly conserved cellular process that participates in the maintenance of intracellular homeostasis through the …             Dynamics of genome change among Legionella species ▶     Sandeep J. Joseph, Daniel Cox, Bernard Wolff et al.     Legionella species inhabit freshwater and soil ecosystems where they parasitize protozoa. L. pneumonphila (LP) serogroup-1 (Lp1) is the major cause of Legionnaires’ Disease (LD), …             Application of RNA-seq for mitogenome reconstruction, and reconsideration of long-branch artifacts in Hemiptera phylogeny ▶     Nan Song, Shiheng An, Xinming Yin et al.     Hemiptera make up the largest nonholometabolan insect assemblage. Despite previous efforts to elucidate phylogeny within this group, relationships among the major sub-lineages …             Entinostat up-regulates the CAMP gene encoding LL-37 via activation of STAT3 and HIF-1α transcription factors ▶     Erica Miraglia, Frank Nylén, Katarina Johansson et al.     Bacterial resistance against classical antibiotics is a growing problem and the development of new antibiotics is limited. Thus, novel alternatives to antibiotics are warranted. …             Slowly dispersing neotenic beetles can speciate on a penny coin and generate space-limited diversity in the tropical mountains ▶     Timothy C. Bray, Ladislav Bocak     We demonstrate the controversial origin of a biological species within an area of a few kilometres in the absence of physical barriers. We employed nuclear rRNA/mitochondrial and …             Chiral Antioxidant-based Gold Nanoclusters Reprogram DNA Epigenetic Patterns ▶     Yue Ma, Hualin Fu, Chunlei Zhang et al.     Epigenetic modifications sit ‘on top of’ the genome and influence DNA transcription, which can force a significant impact on cellular behavior and phenotype and, consequently …             Biochemical and structural characterization of the interface mediating interaction between the influenza A virus non-structural protein-1 and a monoclonal antibody ▶     Jianping Wu, Chee-Keng Mok, Vincent Tak Kwong Chow et al.     We have previously shown that a non-structural protein 1 (NS1)-binding monoclonal antibody, termed as 2H6, can significantly reduce influenza A virus (IAV) replication when …             Molecular basis of a novel renal amyloidosis due to N184K gelsolin variant ▶     Francesco Bonì, Mario Milani, Riccardo Porcari et al.     Mutations in gelsolin are responsible for a systemic amyloidosis first described in 1969. Until recently, the disease was associated with two substitutions of the same residue, …             Mitigation of soil N2O emission by inoculation with a mixed culture of indigenous Bradyrhizobium diazoefficiens ▶     Hiroko Akiyama, Yuko Takada Hoshino, Manabu Itakura et al.     Agricultural soil is the largest source of nitrous oxide (N2O), a greenhouse gas. Soybean is an important leguminous crop worldwide. Soybean hosts symbiotic nitrogen-fixing soil …             External cavity-quantum cascade laser infrared spectroscopy for secondary structure analysis of proteins at low concentrations ▶     Andreas Schwaighofer, Mirta R. Alcaráz, Can Araman et al.     Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy are analytical techniques employed for the analysis of protein secondary structure. The use of CD …             Reprofiled anthelmintics abate hypervirulent stationary-phase Clostridium difficile ▶     Major Gooyit, Kim D. Janda     Prolonged use of broad-spectrum antibiotics disrupts the indigenous gut microbiota, which consequently enables toxigenic Clostridium difficile species to proliferate and cause …             Long-term exposure to microgravity impairs vestibulo-cardiovascular reflex ▶     Hironobu Morita, Chikara Abe, Kunihiko Tanaka     The vestibular system is known to have an important role in controlling blood pressure upon posture transition (vestibulo-cardiovascular reflex, VCR). However, under a different …             Meta-analysis of associations between DLG5 R30Q and P1371Q polymorphisms and susceptibility to inflammatory bowel disease ▶     Yunhai Li, Ping Chen, Jiazheng Sun et al.     Growing evidence from recent studies has demonstrated an association between inflammatory bowel disease (IBD) susceptibility and two polymorphisms of DLG5 R30Q (rs1248696) and …             Structural modulation of the gut microbiota and the relationship with body weight: compared evaluation of liraglutide and saxagliptin treatment ▶     Lin Wang, Peicheng Li, Zhaosheng Tang et al.     The mechanisms underlying the weight-loss effect of GLP-1 receptor agonists need further elucidation. The present study was performed to explore the effects of liraglutide and …             Topical Simvastatin as Host-Directed Therapy against Severity of Cutaneous Leishmaniasis in Mice ▶     Suraj P. Parihar, Mary-Anne Hartley, Ramona Hurdayal et al.     We recently demonstrated that statins mediate protection against intracellular pathogens, Mycobacterium tuberculosis and Listeria monocytogenes in mice. Here, we investigated the …             Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study ▶     Vitalij Novickij, Audrius Grainys, Eglė Lastauskienė et al.     Electroporation is a phenomenon occurring due to exposure of cells to Pulsed Electric Fields (PEF) which leads to increase of membrane permeability. Electroporation is used in …             A Novel Assay for Screening Inhibitors Targeting HIV Integrase LEDGF/p75 Interaction Based on Ni2+ Coated Magnetic Agarose Beads ▶     Zhang Dawei, He Hongqiu, Liu Mengmeng et al.     HIV-1 integrase (IN) plays an essential role in viral replication and thus serves as an important target for chemotherapeutic intervention against HIV-1 infection. However, the …             Copper(II) ions affect the gating dynamics of the 20S proteasome: a molecular and in cell study ▶     Anna Maria Santoro, Irene Monaco, Francesco Attanasio et al.     Due to their altered metabolism cancer cells are more sensitive to proteasome inhibition or changes of copper levels than normal cells. Thus, the development of copper complexes …             Chronic Kappa opioid receptor activation modulates NR2B: Implication in treatment resistant depression ▶     Shalini Dogra, Ajeet Kumar, Deepmala Umrao et al.     Psychotomimetic and prodepressive effect by kappa opioid receptor (KOR) activation in rodents and human is widely known. Significantly, recent clinical investigations …             Ursolic acid-mediated apoptosis of K562 cells involves Stat5/Akt pathway inhibition through the induction of Gfi-1 ▶     Ze Lin, Jikai Jiang, Xiao-Shan Liu     Ursolic acid (UA) is a promising natural compound for cancer prevention and therapy. We previously reported that UA induced apoptosis in CML-derived K562 cells. Here we show that …             Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGFβ)-dependent smooth muscle cell phenotype modulation ▶     Pei-Yu Chen, Lingfeng Qin, Guangxin Li et al.     Smooth muscle cells (SMCs) in normal blood vessels exist in a highly differentiate state characterized by expression of SMC-specific contractile proteins (“contractile …             Potential of in vivo real-time gastric gas profiling: a pilot evaluation of heat-stress and modulating dietary cinnamon effect in an animal model ▶     Jian Zhen Ou, Jeremy J. Cottrell, Nam Ha et al.     Gastroenterologists are still unable to differentiate between some of the most ordinary disorders of the gut and consequently patients are misdiagnosed. We have developed a …             Silencing of Histone Deacetylase 9 Expression in Podocytes Attenuates Kidney Injury in Diabetic Nephropathy ▶     Feng Liu, Ming Zong, Xiaofei Wen et al.     Podocyte dysfunction is important in the onset and development of diabetic nephropathy (DN). Histone deacetylases (HDACs) have been recently proved to play critical roles in the …             A diverse virome in kidney transplant patients contains multiple viral subtypes with distinct polymorphisms ▶     Asha Rani, Ravi Ranjan, Halvor S. McGee et al.     Recent studies have established that the human urine contains a complex microbiome, including a virome about which little is known. Following immunosuppression in kidney …             Reversible Brain Abnormalities in People Without Signs of Mountain Sickness During High-Altitude Exposure ▶     Cunxiu Fan, Yuhua Zhao, Qian Yu et al.     A large proportion of lowlanders ascending to high-altitude (HA) show no signs of mountain sickness. Whether their brains have indeed suffered from HA environment and the …             Eukaryotic pathways targeted by the type III secretion system effector protein, BipC, involved in the intracellular lifecycle of Burkholderia pseudomallei ▶     Wen-Tyng Kang, Kumutha Malar Vellasamy, Jamuna Vadivelu     Burkholderia pseudomallei, the etiological agent for melioidosis, is known to secrete a type III secretion system (TTSS) protein into the host’s internal milieu. One of the TTSS …             Methylcrotonoyl-CoA carboxylase 1 potentiates RLR-induced NF-κB signaling by targeting MAVS complex ▶     Zhongying Cao, Zhangchuan Xia, Yaqin Zhou et al.     RNA virus infections are detected by the RIG-I family of receptors, which signal through the adaptor molecule mitochondrial antiviral signaling (MAVS). MAVS then recruits the …             Determinants of effective lentivirus-driven microRNA expression in vivo ▶     Takuya Mishima, Elena Sadovsky, Margaret E. Gegick et al.     Manipulation of microRNA (miRNA) levels, including overexpression of mature species, has become an important biological tool, even motivating miRNA-based therapeutics. To assess …             Alterations in flowering strategies and sexual allocation of Caragana stenophylla along a climatic aridity gradient ▶     Lina Xie, Hongyu Guo, Chengcang Ma     Plant can alter reproductive strategies for adaptation to different environments. However, alterations in flowering strategies and sexual allocation for the same species growing …             Effects of Propofol General Anesthesia on Olfactory Relearning ▶     Li-Jie Jia, Pei Tang, Nicole R. Brandon et al.     How general anesthesia interferes with sensory processing to cause amnesia remains unclear. Here, we show that activation of a learning-associated immediate early gene in rat …             Genome-wide analysis of long intergenic non-coding RNAs in chickpea and their potential role in flower development ▶     Niraj Khemka, Vikash Kumar Singh, Rohini Garg et al.     Non-coding RNAs constitute a major portion of the transcriptome in most of eukaryotes. Long non-coding transcripts originating from the DNA segment present between the protein …             The famous cultivated mushroom Bailinggu is a separate species of the Pleurotus eryngii species complex ▶     Mengran Zhao, Jinxia Zhang, Qiang Chen et al.     The mushroom of the genus Pleurotus in western China, called Bailinggu, is a precious edible fungus with high economic value. However, its taxonomical position is unclear. Some …             CenH3 evolution reflects meiotic symmetry as predicted by the centromere drive model ▶     František Zedek, Petr Bureš     The centromere drive model explaining rapid evolution of eukaryotic centromeres predicts higher frequency of positive selection acting on centromeric histone H3 (CenH3) in clades …             Extension of the classical classification of β-turns ▶     Alexandre G. de Brevern     The functional properties of a protein primarily depend on its three-dimensional (3D) structure. These properties have classically been assigned, visualized and analysed on the …             Comparative DNA Methylation Profiling Reveals an Immunoepigenetic Signature of HIV-related Cognitive Impairment ▶     Michael J. Corley, Christian Dye, Michelle L. D’Antoni et al.     Monocytes/macrophages contribute to the neuropathogenesis of HIV-related cognitive impairment (CI); however, considerable gaps in our understanding of the precise mechanisms …             In-hive Pesticide Exposome: Assessing risks to migratory honey bees from in-hive pesticide contamination in the Eastern United States ▶     Kirsten S. Traynor, Jeffery S. Pettis, David R. Tarpy et al.     This study measured part of the in-hive pesticide exposome by analyzing residues from live in-hive bees, stored pollen, and wax in migratory colonies over time and compared …             Divergent Effects of Nitrogen Addition on Soil Respiration in a Semiarid Grassland ▶     Cheng Zhu, Yiping Ma, Honghui Wu et al.     Nitrogen (N) deposition has been steadily increasing for decades, with consequences for soil respiration. However, we have a limited understanding of how soil respiration …             STYK1 promotes epithelial-mesenchymal transition and tumor metastasis in human hepatocellular carcinoma through MEK/ERK and PI3K/AKT signaling ▶     Zhaowen Wang, Lei Qu, Biao Deng et al.     Serine/threonine/tyrosine kinase 1 (STYK1) is known to be involved in tumor progression. However, its molecular role and mechanism in hepatocellular carcinoma (HCC) remains …             Difference in leaf water use efficiency/photosynthetic nitrogen use efficiency of Bt-cotton and its conventional peer ▶     Ruqing Guo, Shucun Sun, Biao Liu     This study is to test the effects of Bt gene introduction on the foliar water/nitrogen use efficiency in cotton. We measured leaf stomatal conductance, photosynthetic rate, and …             Tectonic and climatic impacts on the biota within the Red River Fault, evidence from phylogeography of Cycas dolichophylla (Cycadaceae) ▶     Ying Zheng, Jian Liu, Xun Gong     Dramatic crustal deformation and river incision in Southwest China induced by the Indo-Asian collision have long been argued to contribute to the complicated landscapes, …             Synchronous vitellogenin expression and sexual maturation during migration are negatively correlated with juvenile hormone levels in Mythimna separata ▶     Hai-Jun Xiao, Xiao-Wei Fu, Yong-Qiang Liu et al.     Annual migration of pests between different seasonal habitats can lead to serious crop damage. Reproductive immaturity is generally associated with the migratory process …             Double-Network Hydrogel with Tunable Mechanical Performance and Biocompatibility for the Fabrication of Stem Cells-Encapsulated Fibers and 3D Assemble ▶     Zhe Liang, Chenguang Liu, Lili Li et al.     Fabrication of cell-encapsulated fibers could greatly contribute to tissue engineering and regenerative medicine. However, existing methods suffered from not only unavoidability …             Crystal structures of the bifunctional tRNA methyltransferase Trm5a ▶     Caiyan Wang, Qian Jia, Ran Chen et al.     tRNA methyltransferase Trm5 catalyses the transfer of a methyl group from S-adenosyl-L-methionine to G37 in eukaryotes and archaea. The N1-methylated guanosine is the product of …             Effects of captivity and artificial breeding on microbiota in feces of the red-crowned crane (Grus japonensis) ▶     Yuwei Xie, Pu Xia, Hui Wang et al.     Reintroduction of the threatened red-crowned crane has been unsuccessful. Although gut microbiota correlates with host health, there is little information on gut microbiota of …             Patterns of cooperation during collective emergencies in the help-or-escape social dilemma ▶     Mehdi Moussaïd, Mareike Trauernicht     Although cooperation is central to the organisation of many social systems, relatively little is known about cooperation in situations of collective emergency. When groups of …             Identification of a novel sesquiterpene biosynthetic machinery involved in astellolide biosynthesis ▶     Yasutomo Shinohara, Shunji Takahashi, Hiroyuki Osada et al.     Esterified drimane-type sesquiterpene lactones such as astellolides display various biological activities and are widely produced by plants and fungi. Given their low homology to …             Drug-loadable Mesoporous Bioactive Glass Nanospheres: Biodistribution, Clearance, BRL Cellular Location and Systemic Risk Assessment via 45Ca Labelling and Histological Analysis ▶     Baiyan Sui, Gaoren Zhong, Jiao Sun     Mesoporous bioactive glass (MBG) nanospheres with excellent drug loading property have attracted significant attention in the field of nano-medicine. However, systemic metabolism …             Long-term adaptation of the influenza A virus by escaping cytotoxic T-cell recognition ▶     Rutger G. Woolthuis, Christiaan H. van Dorp, Can Keşmir et al.     The evolutionary adaptation of the influenza A virus (IAV) to human antibodies is well characterised. Much less is known about the long-term evolution of cytotoxic T lymphocyte …             Identification of differentially expressed genes in Monochamus alternatus digested with azadirachtin ▶     Tong Lin, Qisi Liu, Jingxiang Chen     The pine sawyer beetle Monochamus alternatus Hope, a major forest insect pest, is the primary vector of the destructive forest pest pine wood nematode, Bursaphelenchus xylophilus…             Acoustic indices provide information on the status of coral reefs: an example from Moorea Island in the South Pacific ▶     Frédéric Bertucci, Eric Parmentier, Gaël Lecellier et al.     Different marine habitats are characterised by different soundscapes. How or which differences may be representative of the habitat characteristics and/or community structure …             Catalytic Thr or Ser Residue Modulates Structural Switches in 2-Cys Peroxiredoxin by Distinct Mechanisms ▶     Carlos A. Tairum, Melina Cardoso Santos, Carlos A. Breyer et al.     Typical 2-Cys Peroxiredoxins (2-Cys Prxs) reduce hydroperoxides with extraordinary rates due to an active site composed of a catalytic triad, containing a peroxidatic cysteine (CP…             Morphological abnormalities and cell death in the Asian citrus psyllid (Diaphorina citri) midgut associated with Candidatus Liberibacter asiaticus ▶     Murad Ghanim, Somayeh Fattah-Hosseini, Amit Levy et al.     Candidatus Liberibacter asiaticus (CLas) is a phloem-limited, gram-negative, fastidious bacterium that is associated with the development of citrus greening disease, also known …             Focused Ultrasound-Induced Blood-Brain Barrier Opening: Association with Mechanical Index and Cavitation Index Analyzed by Dynamic Contrast-Enhanced Magnetic-Resonance Imaging ▶     Po-Chun Chu, Wen-Yen Chai, Chih-Hung Tsai et al.     Focused ultrasound (FUS) with microbubbles can temporally open the blood-brain barrier (BBB), and the cavitation activities of microbubbles play a key role in the BBB-opening …             Molecular Dynamics Simulations of the Permeation of Bisphenol A and Pore Formation in a Lipid Membrane ▶     Liang Chen, Junlang Chen, Guoquan Zhou et al.     Bisphenol A (BPA) is particularly considered as one of the most suspicious endocrine disruptors. Exposure to BPA may bring about possible human toxicities, such as cancerous …             α-Synuclein is a Novel Microtubule Dynamase ▶     Daniele Cartelli, Alessandro Aliverti, Alberto Barbiroli et al.     α-Synuclein is a presynaptic protein associated to Parkinson’s disease, which is unstructured when free in the cytoplasm and adopts α helical conformation when bound to vesicles. …             Low genetic diversity and recent demographic expansion in the red starfish Echinaster sepositus (Retzius 1816) ▶     Alex Garcia-Cisneros, Creu Palacín, Yousra Ben Khadra et al.     Understanding the phylogeography and genetic structure of populations and the processes responsible of patterns therein is crucial for evaluating the vulnerability of marine …             Primates’ behavioural responses to tourists: evidence for a trade-off between potential risks and benefits ▶     Laëtitia Maréchal, Ann MacLarnon, Bonaventura Majolo et al.     The presence of, and interactions with tourists can be both risky and beneficial for wild animals. In wildlife tourism settings, animals often experience elevated rates of …             Investigating the Responses of Human Epithelial Cells to Predatory Bacteria ▶     Ajay K. Monnappa, Wasimul Bari, Seong Yeol Choi et al.     One beguiling alternative to antibiotics for treating multi-drug resistant infections are Bdellovibrio-and-like-organisms (BALOs), predatory bacteria known to attack human …             DNA damage and Repair Modify DNA methylation and Chromatin Domain of the Targeted Locus: Mechanism of allele methylation polymorphism ▶     Giusi Russo, Rosaria Landi, Antonio Pezone et al.     We characterize the changes in chromatin structure, DNA methylation and transcription during and after homologous DNA repair (HR). We find that HR modifies the DNA methylation …             siRNA capsulated brain-targeted nanoparticles specifically knock down OATP2B1 in mice: a mechanism for acute morphine tolerance suppression ▶     Zi-Zhao Yang, Li Li, Lu Wang et al.     Regulating main brain-uptake transporter of morphine may restrict its tolerance generation, then modify its antinociception. In this study, more than 2 fold higher intracellular …             Adipokinetic hormones and their G protein-coupled receptors emerged in Lophotrochozoa ▶     Shizhong Li, Frank Hauser, Signe K. Skadborg et al.     Most multicellular animals belong to two evolutionary lineages, the Proto– and Deuterostomia, which diverged 640–760 million years (MYR) ago. Neuropeptide signaling is abundant …             Unique roles of the unfolded protein response pathway in fungal development and differentiation ▶     Kwang-Woo Jung, Yee-Seul So, Yong-Sun Bahn     Cryptococcus neoformans, a global fungal meningitis pathogen, employs the unfolded protein response pathway. This pathway, which consists of an evolutionarily conserved Ire1 …             Postsynaptic SDC2 induces transsynaptic signaling via FGF22 for bidirectional synaptic formation ▶     Hsiao-Tang Hu, Hisashi Umemori, Yi-Ping Hsueh     Functional synapse formation requires tight coordination between pre- and post-synaptic termini. Previous studies have shown that postsynaptic expression of heparan sulfate …             The Tetraodon nigroviridis reference transcriptome: developmental transition, length retention and microsynteny of long non-coding RNAs in a compact vertebrate genome ▶     Swaraj Basu, Yavor Hadzhiev, Giuseppe Petrosino et al.     Pufferfish such as fugu and tetraodon carry the smallest genomes among all vertebrates and are ideal for studying genome evolution. However, comparative genomics using these …             Fine-tuning of amino sugar homeostasis by EIIANtr in Salmonella Typhimurium ▶     Woongjae Yoo, Hyunjin Yoon, Yeong-Jae Seok et al.     The nitrogen-metabolic phosphotransferase system, PTSNtr, consists of the enzymes INtr, NPr and IIANtr that are encoded by ptsP, ptsO, and ptsN, respectively. Due to the …             Amyloid structure exhibits polymorphism on multiple length scales in human brain tissue ▶     Jiliang Liu, Isabel Costantino, Nagarajan Venugopalan et al.     Aggregation of Aβ amyloid fibrils into plaques in the brain is a universal hallmark of Alzheimer’s Disease (AD), but whether plaques in different individuals are equivalent is …             Global analysis of differential gene expression related to long-term sperm storage in oviduct of Chinese Soft-Shelled Turtle Pelodiscus sinensis ▶     Tengfei Liu, Ping Yang, Hong Chen et al.     Important evolutionary and ecological consequences arise from the ability of female turtles to store viable spermatozoa for an extended period. Although previous morphological …             Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation ▶     Shunsuke Kimura, Masami Yamashita, Megumi Yamakami-Kimura et al.     The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and …             SELMAP - SELEX affinity landscape MAPping of transcription factor binding sites using integrated microfluidics ▶     Dana Chen, Yaron Orenstein, Rada Golodnitsky et al.     Transcription factors (TFs) alter gene expression in response to changes in the environment through sequence-specific interactions with the DNA. These interactions are best …             Prolonged California aridity linked to climate warming and Pacific sea surface temperature ▶     Glen M. MacDonald, Katrina A. Moser, Amy M. Bloom et al.     California has experienced a dry 21st century capped by severe drought from 2012 through 2015 prompting questions about hydroclimatic sensitivity to anthropogenic climate change …             Y chromosome haplogroups based genome-wide association study pinpoints revelation for interactions on non-obstructive azoospermia ▶     Chuncheng Lu, Yang Wen, Weiyue Hu et al.     The Y chromosome has high genetic variability with low rates of parallel and back mutations, which make up the most informative haplotyping system. To examine whether Y …             Anion channelrhodopsins for inhibitory cardiac optogenetics ▶     Elena G. Govorunova, Shane R. Cunha, Oleg A. Sineshchekov et al.     Optical control of the heart muscle is a promising strategy for cardiology because it is more specific than traditional electrical stimulation, and allows a higher temporal …             Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models ▶     Felipe A. Navarrete, Antonio Alvau, Hoi Chang Lee et al.     Mammalian sperm acquire fertilizing capacity in the female tract in a process called capacitation. At the molecular level, capacitation requires protein kinase A activation, …             Tlr4-mutant mice are resistant to acute alcohol-induced sterol-regulatory element binding protein activation and hepatic lipid accumulation ▶     Zhi-Hui Zhang, Xiao-Qian Liu, Cheng Zhang et al.     Previous studies demonstrated that acute alcohol intoxication caused hepatic lipid accumulation. The present study showed that acute alcohol intoxication caused hepatic lipid …             BG-4, a novel anticancer peptide from bitter gourd (Momordica charantia), promotes apoptosis in human colon cancer cells ▶     Vermont P. Dia, Hari B. Krishnan     Momordica charantia is a perennial plant with reported health benefits. BG-4, a novel peptide from Momordica charantia, was isolated, purified and characterized. The trypsin …             The fungicide triadimefon affects beer flavor and composition by influencing Saccharomyces cerevisiae metabolism ▶     Zhiqiang Kong, Minmin Li, Jingjing An et al.     Despite the fact that beer is produced on a large scale, the effects of pesticide residues on beer have been rarely investigated. In this study, we used micro-brewing settings to …             Telocytes in gastric lamina propria of the Chinese giant salamander, Andrias davidianus ▶     Hui Zhang, Shengwei Zhong, Pengcheng Yu et al.     In this study, we attempt to identify gastric telocytes (TCs) of the Chinese giant salamander Andrias davidianus, by light microscopy, immunohistochemistry and transmission …             The coiled-coil domain of zebrafish TRPM7 regulates Mg·nucleotide sensitivity ▶     Chad Jansen, Jaya Sahni, Sayuri Suzuki et al.     TRPM7 is a member of the Transient-Receptor-Potential Melastatin ion channel family. TRPM7 is a unique fusion protein of an ion channel and an α-kinase. Although mammalian TRPM7 …             The Impact of Cognitive Training on Cerebral White Matter in Community-Dwelling Elderly: One-Year Prospective Longitudinal Diffusion Tensor Imaging Study ▶     Xinyi Cao, Ye Yao, Ting Li et al.     It has been shown that cognitive training (CogTr) is effective and recuperative for older adults, and can be used to fight against cognitive decline. In this study, we …             Analysis of long non-coding RNA expression profiles in pancreatic ductal adenocarcinoma ▶     Xue-Liang Fu, De-Jun Liu, Ting-Ting Yan et al.     Pancreatic ductal adenocarcinoma (PDAC) remains one of the most aggressive and lethal malignancies. Long non-coding RNAs (lncRNAs) are a novel class of non-protein-coding …             Diagnostic copper imaging of Menkes disease by synchrotron radiation-generated X-ray fluorescence analysis ▶     Miyuki Kinebuchi, Akihiro Matsuura, Tohru Kiyono et al.     Copper (Cu) is an indispensable metal for normal development and function of humans, especially in central nervous system (CNS). However, its redox activity requires accurate Cu …             Oncogenic Mutations Differentially Affect Bax Monomer, Dimer, and Oligomeric Pore Formation in the Membrane ▶     Mingzhen Zhang, Jie Zheng, Ruth Nussinov et al.     Dysfunction of Bax, a pro-apoptotic regulator of cellular metabolism is implicated in neurodegenerative diseases and cancer. We have constructed the first atomistic models of the …             MDM2 promoter SNP55 (rs2870820) affects risk of colon cancer but not breast-, lung-, or prostate cancer ▶     Reham Helwa, Liv B. Gansmo, Pål Romundstad et al.     Two functional SNPs (SNP285G > C; rs117039649 and SNP309T > G; rs2279744) have previously been reported to modulate Sp1 transcription factor binding to the promoter of the …             Corrigendum: Genome-wide analysis of tomato long non-coding RNAs and identification as endogenous target mimic for microRNA in response to TYLCV infection ▶     Jinyan Wang, Wengui Yu, Yuwen Yang et al.                 Corrigendum: Identification of cancer-driver genes in focal genomic alterations from whole genome sequencing data ▶     Ho Jang, Youngmi Hur, Hyunju Lee                 Chemokine CXCL1 mediated neutrophil recruitment: Role of glycosaminoglycan interactions ▶     Kirti V. Sawant, Krishna Mohan Poluri, Amit K. Dutta et al.     The chemokine CXCL1/MGSA plays a pivotal role in the host immune response by recruiting and activating neutrophils for microbial killing at the tissue site. CXCL1 exists …             Complement C5a induces PD-L1 expression and acts in synergy with LPS through Erk1/2 and JNK signaling pathways ▶     Ling-Ling An, Jacob V. Gorman, Geoffrey Stephens et al.     Severe bacterial infection results in both uncontrolled inflammation and immune suppression in septic patients. Although there is ample evidence that complement activation …             Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon ▶     Tianda Li, Ling Shuai, Junjie Mao et al.     Rats with fluorescent markers are of great value for studies that trace lineage-specific development, particularly those assessing the differentiation potential of embryonic stem …             Panel-Based Population Next-Generation Sequencing for Inherited Retinal Degenerations ▶     Matthew Carrigan, Emma Duignan, Conor P. G. Malone et al.     Inherited retinopathies affect approximately two and a half million people globally, yet the majority of affected patients lack clear genetic diagnoses given the diverse range of …             Integrin α6/Akt/Erk signaling is essential for human breast cancer resistance to radiotherapy ▶     Ting Hu, Rui Zhou, Yanxia Zhao et al.     Integrin α6 (ITGA6), a transmembrane glycoprotein adhesion receptor protein, is widely upregulated in many types of tumors and promotes migration and invasion in cancer cells. …             Corrigendum: Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells ▶     Yu-Che Cheng, Chi-Jung Huang, Yih-Jing Lee et al.                 Refinement of the androgen response element based on ChIP-Seq in androgen-insensitive and androgen-responsive prostate cancer cell lines ▶     Stephen Wilson, Jianfei Qi, Fabian V. Filipp     Sequence motifs are short, recurring patterns in DNA that can mediate sequence-specific binding for proteins such as transcription factors or DNA modifying enzymes. The androgen …             The Crystal Structure of Peroxiredoxin Asp f3 Provides Mechanistic Insight into Oxidative Stress Resistance and Virulence of Aspergillus fumigatus ▶     Falk Hillmann, Karine Bagramyan, Maria Straßburger et al.     Invasive aspergillosis and other fungal infections occur in immunocompromised individuals, including patients who received blood-building stem cell transplants, patients with …             Mechanistic Modelling of DNA Repair and Cellular Survival Following Radiation-Induced DNA Damage ▶     Stephen J. McMahon, Jan Schuemann, Harald Paganetti et al.     Characterising and predicting the effects of ionising radiation on cells remains challenging, with the lack of robust models of the underlying mechanism of radiation responses …             Thiol dependent intramolecular locking of Orai1 channels ▶     Dalia Alansary, Barbara Schmidt, Kathrin Dörr et al.     Store-operated Ca2+ entry mediated by STIM1-gated Orai1 channels is essential to activate immune cells and its inhibition or gain-of-function can lead to immune dysfunction and …             Future climate stimulates population out-breaks by relaxing constraints on reproduction ▶     Katherine A. Heldt, Sean D. Connell, Kathryn Anderson et al.     When conditions are stressful, reproduction and population growth are reduced, but when favourable, reproduction and population size can boom. Theory suggests climate change is …             Comparative analysis of linker histone H1, MeCP2, and HMGD1 on nucleosome stability and target site accessibility ▶     Caitlyn Riedmann, Yvonne N. Fondufe-Mittendorf     Chromatin architectural proteins (CAPs) bind the entry/exit DNA of nucleosomes and linker DNA to form higher order chromatin structures with distinct transcriptional outcomes. …             An APOC3 3′UTR variant associated with plasma triglycerides levels and coronary heart disease by creating a functional miR-4271 binding site ▶     Sen-Lin Hu, Guang-Lin Cui, Jin Huang et al.     Apolipoprotein C-III (APOC3) is a key regulator of plasma triglycerides levels. Increasing evidence has shown that loss-of-function mutations in APOC3 is associated with …             The enhanced atorvastatin hepatotoxicity in diabetic rats was partly attributed to the upregulated hepatic Cyp3a and SLCO1B1 ▶     Nan Shu, Mengyue Hu, Zhaoli Ling et al.     Liver injury is a common adverse effect of atorvastatin. This study aimed to investigate atorvastatin-induced hepatotoxicity in diabetic rats induced by high-fat diet combined …             Splenic CD11clowCD45RBhigh dendritic cells derived from endotoxin-tolerant mice attenuate experimental acute liver failure ▶     Sai-Nan Zhang, Nai-Bin Yang, Shun-Lan Ni et al.     Endotoxin tolerance (ET) is suggested to attenuate the severity of acute liver failure (ALF) in mice, possibly through both innate and adaptive immunity. However, the involvement …             Subpathway-CorSP: Identification of metabolic subpathways via integrating expression correlations and topological features between metabolites and genes of interest within pathways ▶     Chenchen Feng, Jian Zhang, Xuecang Li et al.     Metabolic pathway analysis is a popular strategy for comprehensively researching metabolites and genes of interest associated with specific diseases. However, the traditional …             Gene deleted live attenuated Leishmania vaccine candidates against visceral leishmaniasis elicit pro-inflammatory cytokines response in human PBMCs ▶     Kumar Avishek, Himanshu Kaushal, Sreenivas Gannavaram et al.     Currently no effective vaccine is available for human visceral leishmaniasis(VL) caused by Leishmania donovani. Previously, we showed that centrin1 and p27gene deleted live …             Imaging the antimicrobial mechanism(s) of cathelicidin-2 ▶     Viktoria A. F. Schneider, Maarten Coorens, Soledad R. Ordonez et al.     Host defence peptides (HDPs) have the potential to become alternatives to conventional antibiotics in human and veterinary medicine. The HDP chicken cathelicidin-2 (CATH-2) has …             The Use of a Novel NanoLuc -Based Reporter Phage for the Detection of Escherichia coli O157:H7 ▶     Dandan Zhang, Claudia P. Coronel-Aguilera, Patricia L. Romero et al.     Rapid detection of the foodborne pathogen Escherichia coli O157:H7 is of vital importance for public health worldwide. Among detection methods, reporter phages represent unique …             Hook2, a microtubule-binding protein, interacts with Par6α and controls centrosome orientation during polarized cell migration ▶     Emilie Pallesi-Pocachard, Elsa Bazellieres, Annelise Viallat-Lieutaud et al.     Polarity protein complexes function during polarized cell migration and a subset of these proteins localizes to the reoriented centrosome during this process. Despite these …             Allergy-specific Phenome-Wide Association Study for Immunogenes in Turkish Children ▶     Sefayet Karaca, Ersoy Civelek, Mehmet Karaca et al.     To dissect the role of immunogenetics in allergy and asthma, we performed a phenome-wide association study in 974 Turkish children selected from a cross-sectional study conducted …             Deep brain stimulation of the subthalamic nucleus modulates sensitivity to decision outcome value in Parkinson’s disease ▶     Ben Seymour, Michael Barbe, Peter Dayan et al.     Deep brain stimulation (DBS) of the subthalamic nucleus in Parkinson’s disease is known to cause a subtle but important adverse impact on behaviour, with impulsivity its most …             Virulence Differences among Melissococcus plutonius Strains with Different Genetic Backgrounds in Apis mellifera Larvae under an Improved Experimental Condition ▶     Keiko Nakamura, Yuko Yamazaki, Akiyo Shiraishi et al.     European foulbrood (EFB) caused by Melissococcus plutonius is an important bacterial disease of honeybee larvae. M. plutonius strains can be grouped into three genetically …             Double-layered cell transfer technology for bone regeneration ▶     Keiko Akazawa, Kengo Iwasaki, Mizuki Nagata et al.     For cell-based medicine, to mimic in vivo cellular localization, various tissue engineering approaches have been studied to obtain a desirable arrangement of cells on scaffold …             Characterization of human mitochondrial ferritin promoter: identification of transcription factors and evidences of epigenetic control ▶     Michela Guaraldo, Paolo Santambrogio, Elisabetta Rovelli et al.     Mitochondrial ferritin (FtMt) is an iron storage protein belonging to the ferritin family but, unlike the cytosolic ferritin, it has an iron-unrelated restricted tissue …             Redox/methylation mediated abnormal DNA methylation as regulators of ambient fine particulate matter-induced neurodevelopment related impairment in human neuronal cells ▶     Hongying Wei, Fan Liang, Ge Meng et al.     Fine particulate matter (PM2.5) has been implicated as a risk factor for neurodevelopmental disorders including autism in children. However, the underlying biological mechanism …             The PCP pathway regulates Baz planar distribution in epithelial cells ▶     Benoit Aigouy, André Le Bivic     The localisation of apico-basal polarity proteins along the Z-axis of epithelial cells is well understood while their distribution in the plane of the epithelium is poorly …             Erratum: Advances in mapping malaria for elimination: fine resolution modelling of Plasmodium falciparum incidence ▶     Victor A. Alegana, Peter M. Atkinson, Christopher Lourenço et al.                 Fecal metabolome of the Hadza hunter-gatherers: a host-microbiome integrative view ▶     Silvia Turroni, Jessica Fiori, Simone Rampelli et al.     The recent characterization of the gut microbiome of traditional rural and foraging societies allowed us to appreciate the essential co-adaptive role of the microbiome in …             Amyloidogenic amyloid-β-peptide variants induce microbial agglutination and exert antimicrobial activity ▶     Philipp Spitzer, Mateja Condic, Martin Herrmann et al.     Amyloid-β (Aβ) peptides are the main components of the plaques found in the brains of patients with Alzheimer’s disease. However, Aβ peptides are also detectable in secretory …             Attention induced neural response trade-off in retinotopic cortex under load ▶     Ana Torralbo, Todd A. Kelley, Geraint Rees et al.     The effects of perceptual load on visual cortex response to distractors are well established and various phenomena of ‘inattentional blindness’ associated with elimination of …             Simple and rapid detection of Tilletia horrida causing rice kernel smut in rice seeds ▶     Yu Chen, Xue Yang, Jian Yao et al.     A simple and rapid method for the detection of Tilletia horrida, the causal agent of rice kernel smut, in rice seeds is developed based on specific polymerase chain reaction …             Visible-absorption spectroscopy as a biomarker to predict treatment response and prognosis of surgically resected esophageal cancer ▶     Pei-Wen Yang, I-Jen Hsu, Chun-Wei Chang et al.     The application of optical absorption spectra in prognostic prediction has hardly been investigated. We developed and evaluated a novel two dimensional absorption spectrum …             Wild capuchin monkeys adjust stone tools according to changing nut properties ▶     Lydia V. Luncz, Tiago Falótico, Alejandra Pascual-Garrido et al.     Animals foraging in their natural environments need to be proficient at recognizing and responding to changes in food targets that affect accessibility or pose a risk. Wild …             High-throughput sequencing-based analysis of endogenetic fungal communities inhabiting the Chinese Cordyceps reveals unexpectedly high fungal diversity ▶     Fei Xia, Xin Chen, Meng-Yuan Guo et al.     Chinese Cordyceps, known in Chinese as “DongChong XiaCao”, is a parasitic complex of a fungus (Ophiocordyceps sinensis) and a caterpillar. The current study explored the …             Synthesis and mechanisms of action of novel harmine derivatives as potential antitumor agents ▶     Xiao-Fei Zhang, Rong-qin Sun, Yi-fan Jia et al.     A series of novel harmine derivatives bearing a benzylindine substituent in position-1 of β-carboline ring were synthesized and evaluated as antitumor agents. The N2-benzylated …             A Rapid and Efficient Luminescence-based Method for Assaying Phosphoglycosyltransferase Enzymes ▶     Debasis Das, Marthe. T. C. Walvoort, Vinita Lukose et al.     Phosphoglycosyltransferases (PGTs) are families of integral membrane proteins with intriguingly diverse architectures. These enzymes function to initiate many important …             Bexarotene targets autophagy and is protective against thromboembolic stroke in aged mice with tauopathy ▶     Mikko T. Huuskonen, Sanna Loppi, Hiramani Dhungana et al.     Stroke is a highly debilitating, often fatal disorder for which current therapies are suitable for only a minor fraction of patients. Discovery of novel, effective therapies is …             An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea ▶     Yanling Liu, Zhongjuan Zhao, Zheyong Xue et al.     Paralogous members of the oxidosqualene cyclase (OSC) family encode a diversity of enzymes that are important in triterpenoid biosynthesis. This report describes the isolation of …             Rational engineering of a virulence gene from Mycobacterium tuberculosis facilitates proteomic analysis of a natural protein N-terminus ▶     Cristal Reyna, Felix Mba Medie, Matthew M. Champion et al.     Mass spectrometry (MS) for the detection of proteins is an indispensable tool for evaluating the biological processes of the proteome. Proteomics frequently requires proteolysis …             Regulatory T cells control strain specific resistance to Experimental Autoimmune Prostatitis ▶     Maria L. Breser, Andreia C. Lino, Ruben D. Motrich et al.     Susceptibility to autoimmune diseases results from the encounter of a complex and long evolved genetic context with a no less complex and changing environment. Major actors in …             Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function ▶     Robert J. Lee, Alan D. Workman, Ryan M. Carey et al.     Aflatoxins are mycotoxins secreted by Aspergillus flavus, which can colonize the respiratory tract and cause fungal rhinosinusitis or bronchopulmonary aspergillosis. A. flavus is …             P2X-mediated AMPA receptor internalization and synaptic depression is controlled by two CaMKII phosphorylation sites on GluA1 in hippocampal neurons ▶     Johan-Till Pougnet, Benjamin Compans, Audrey Martinez et al.     Plasticity at excitatory synapses can be induced either by synaptic release of glutamate or the release of gliotransmitters such as ATP. Recently, we showed that postsynaptic …             QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems ▶     Ying Zhang, Catherine L. Thomas, Jinxia Xiang et al.     A high-density SNP-based genetic linkage map was constructed and integrated with a previous map in the Tapidor x Ningyou7 (TNDH) Brassica napus population, giving a new map with …             TRPM4-mediated control of FcεRI-evoked Ca2+ elevation comprises enhanced plasmalemmal trafficking of TRPM4 channels in connective tissue type mast cells ▶     Torben Rixecker, Ilka Mathar, Rebekka Medert et al.     TRPM4 proteins form Ca2+-activated non selective cation (CAN) channels that affect transmembrane Ca2+-influx by determining the membrane potential. Tight control of the …             Two-Dimensional Fluorescence Difference Spectroscopy to Characterize Nanoparticles and their Interactions ▶     Miranda N. Hurst, Robert K. DeLong     Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a …             Erratum: Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function ▶     Andre Kleensang, Marguerite M. Vantangoli, Shelly Odwin-DaCosta et al.                 Identification of Viruses in Cases of Pediatric Acute Encephalitis and Encephalopathy Using Next-Generation Sequencing ▶     Jun-ichi Kawada, Yusuke Okuno, Yuka Torii et al.     Acute encephalitis/encephalopathy is a severe neurological syndrome that is occasionally associated with viral infection. Comprehensive virus detection assays are desirable …             Role of anterior piriform cortex in the acquisition of conditioned flavour preference ▶     Cristina Mediavilla, Mar Martin-Signes, Severiano Risco     Flavour aversion learning (FAL) and conditioned flavour preference (CFP) facilitate animal survival and play a major role in food selection, but the neurobiological mechanisms …             A super gene expression system enhances the anti-glioma effects of adenovirus-mediated REIC/Dkk-3 gene therapy ▶     Tetsuo Oka, Kazuhiko Kurozumi, Yosuke Shimazu et al.     Reduced expression in immortalized cells/Dickkopf-3 (REIC/Dkk-3) is a tumor suppressor and therapeutic gene in many human cancers. Recently, an adenovirus REIC vector with the …             Mitochondrial dysfunction is an important cause of neurological deficits in an inflammatory model of multiple sclerosis ▶     Mona Sadeghian, Vincenzo Mastrolia, Ali Rezaei Haddad et al.     Neuroinflammation can cause major neurological dysfunction, without demyelination, in both multiple sclerosis (MS) and a mouse model of the disease (experimental autoimmune …             Contamination-controlled high-throughput whole genome sequencing for influenza A viruses using the MiSeq sequencer ▶     Hong Kai Lee, Chun Kiat Lee, Julian Wei-Tze Tang et al.     Accurate full-length genomic sequences are important for viral phylogenetic studies. We developed a targeted high-throughput whole genome sequencing (HT-WGS) method for influenza …             Genetically Low Vitamin D Levels, Bone Mineral Density, and Bone Metabolism Markers: a Mendelian Randomisation Study ▶     Shan-Shan Li, Li-Hong Gao, Xiao-Ya Zhang et al.     Low serum 25-hydroxyvitamin D (25OHD) is associated with osteoporosis and osteoporotic fracture, but it remains uncertain whether these associations are causal. We conducted a …             An integrative study identifies KCNC2 as a novel predisposing factor for childhood obesity and the risk of diabetes in the Korean population ▶     Joo-Yeon Hwang, Hyo Jung Lee, Min Jin Go et al.     Obesity is a major risk factor for type 2 diabetes. To unravel the genetic determinants of obesity-associated diabetes, we performed a genome-wide study using the 1,000 …             Enteric neurons from Parkinson’s disease patients display ex vivo aberrations in mitochondrial structure ▶     A. S. Baumuratov, P. M. A. Antony, M. Ostaszewski et al.     Based on autopsy material mitochondrial dysfunction has been proposed being part of the pathophysiological cascade of Parkinson’s disease (PD). However, in living patients, …             ASPP2 involvement in p53-mediated HIV-1 envelope glycoprotein gp120 neurotoxicity in mice cerebrocortical neurons ▶     Zhiying Liu, Yunjin Zang, Luxin Qiao et al.     The mechanisms behind HIV-1-associated neurocognitive disorders are still unclear. Apoptosis-stimulating protein 2 of p53 (ASPP2) is a damage-inducible p53-binding protein that …             Acetazolamide Mitigates Astrocyte Cellular Edema Following Mild Traumatic Brain Injury ▶     Nasya M. Sturdivant, Sean G. Smith, Syed F. Ali et al.     Non-penetrating or mild traumatic brain injury (mTBI) is commonly experienced in accidents, the battlefield and in full-contact sports. Astrocyte cellular edema is one of the …             GLITTER: a web-based application for gene link inspection through tissue-specific coexpression ▶     Xiangtao Liu, Pengfei Yu, Chao Cheng et al.     Accumulating evidence supports the polygenic nature of most complex diseases, suggesting the involvement of many susceptibility genes with small effect sizes. Although hundreds …             Curcumin protects ANIT-induced cholestasis through signaling pathway of FXR-regulated bile acid and inflammation ▶     Fan Yang, Xiaowen Tang, Lili Ding et al.     Cholestasis is a clinically significant symptom and widely associated with liver diseases, however, there are very few effective therapies for cholestasis. Danning tablet (DNT, a …             Skin scarification with Plasmodium falciparum peptide vaccine using synthetic TLR agonists as adjuvants elicits malaria sporozoite neutralizing immunity ▶     Robert A. Mitchell, Rita Altszuler, Ute Frevert et al.     Malaria eradication will require a combination of vector control, chemotherapy and an easily administered vaccine. Sterile immunity can be elicited in humans by immunization with …             NOX2 amplifies acetaldehyde-mediated cardiomyocyte mitochondrial dysfunction in alcoholic cardiomyopathy ▶     Moritz Brandt, Venkata Garlapati, Matthias Oelze et al.     Alcoholic cardiomyopathy (ACM) resulting from excess alcohol consumption is an important cause of heart failure (HF). Although it is assumed that the cardiotoxicity of the …             Persistent inflammatory state after photoreceptor loss in an animal model of retinal degeneration ▶     Agustina Noailles, Victoria Maneu, Laura Campello et al.     Microglia act as the resident immune cells of the central nervous system, including the retina. In response to damaging stimuli microglia adopt an activated state, which can …             Residue Geometry Networks: A Rigidity-Based Approach to the Amino Acid Network and Evolutionary Rate Analysis ▶     Alexander S. Fokas, Daniel J. Cole, Sebastian E. Ahnert et al.     Amino acid networks (AANs) abstract the protein structure by recording the amino acid contacts and can provide insight into protein function. Herein, we describe a novel AAN …             A HTML5 open source tool to conduct studies based on Libet’s clock paradigm ▶     Pablo Garaizar, Carmelo P. Cubillas, Helena Matute     Libet’s clock is a well-known procedure in experiments in psychology and neuroscience. Examples of its use include experiments exploring the subjective sense of agency, …             Voluntary movement affects simultaneous perception of auditory and tactile stimuli presented to a non-moving body part ▶     Qiao Hao, Hiroki Ora, Ken-ichiro Ogawa et al.     The simultaneous perception of multimodal sensory information has a crucial role for effective reactions to the external environment. Voluntary movements are known to …             Does cryptic microbiota mitigate pine resistance to an invasive beetle-fungus complex? Implications for invasion potential ▶     Chihang Cheng, Letian Xu, Dandan Xu et al.     Microbial symbionts are known to assist exotic pests in their colonization of new host plants. However, there has been little evidence linking symbiotic invasion success to …             PPM1D controls nucleolar formation by up-regulating phosphorylation of nucleophosmin ▶     Yuuki Kozakai, Rui Kamada, Junya Furuta et al.     An increase of nucleolar number and size has made nucleoli essential markers for cytology and tumour development. However, the underlying basis for their structural integrity and …             πSPIM: high NA high resolution isotropic light-sheet imaging in cell culture dishes ▶     Patrick Theer, Denitsa Dragneva, Michael Knop     Light-sheet fluorescence microscopy (LSFM), also termed single plane illumination microscopy (SPIM), enables live cell fluorescence imaging with optical sectioning capabilities …             Probing the luminal microenvironment of reconstituted epithelial microtissues ▶     Alec E. Cerchiari, Karen E. Samy, Michael E. Todhunter et al.     Polymeric microparticles can serve as carriers or sensors to instruct or characterize tissue biology. However, incorporating microparticles into tissues for in vitro assays …             Model suggests potential for Porites coral population recovery after removal of anthropogenic disturbance (Luhuitou, Hainan, South China Sea) ▶     Meixia Zhao, Bernhard Riegl, Kefu Yu et al.     Population models are important for resource management and can inform about potential trajectories useful for planning purposes, even with incomplete monitoring data. From size …             Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport ▶     Menghua Li, Yan Diao, Ying Liu et al.     Cholesterol is essential for all animal life. However, a high level of cholesterol in the body is strongly associated with the progression of various severe diseases. In our …             EGFP oligomers as natural fluorescence and hydrodynamic standards ▶     György Vámosi, Norbert Mücke, Gabriele Müller et al.     EGFP oligomers are convenient standards for experiments on fluorescent protein-tagged biomolecules. In this study, we characterized their hydrodynamic and fluorescence …             Increased water salinity applied to tomato plants accelerates the development of the leaf miner Tuta absoluta through bottom-up effects ▶     Peng Han, Zhi-jian Wang, Anne-Violette Lavoir et al.     Variation in resource inputs to plants may trigger bottom-up effects on herbivorous insects. We examined the effects of water input: optimal water vs. limited water; water …             Matrix metalloproteinase inhibitor, doxycycline and progression of calcific aortic valve disease in hyperlipidemic mice ▶     Jae-Joon Jung, Mahmoud Razavian, Hye-Yeong Kim et al.     Calcific aortic valve disease (CAVD) is the most common cause of aortic stenosis. Currently, there is no non-invasive medical therapy for CAVD. Matrix metalloproteinases (MMPs) …             Development of a SNP array and its application to genetic mapping and diversity assessment in pepper (Capsicum spp.) ▶     Jiaowen Cheng, Cheng Qin, Xin Tang et al.     The development and application of single nucleotide polymorphisms (SNPs) is in its infancy for pepper. Here, a set of 15,000 SNPs were chosen from the resequencing data to …             Improving the performance of the PLB index for ligand-binding site prediction using dihedral angles and the solvent-accessible surface area ▶     Chen Cao, Shutan Xu     Protein ligand-binding site prediction is highly important for protein function determination and structure-based drug design. Over the past twenty years, dozens of computational …             Changes in intracellular copper concentration and copper-regulating gene expression after PC12 differentiation into neurons ▶     Yasumitsu Ogra, Aya Tejima, Naohiro Hatakeyama et al.     It is suspected that some neurodegenerative diseases are a result of the disturbance of copper (Cu) homeostasis, although it remains unclear whether the disturbance of Cu …             Sterilization Effects of Adult-targeted Baits Containing Insect Growth Regulators on Delia antiqua ▶     Fangyuan Zhou, Guodong Zhu, Haipeng Zhao et al.     The onion maggot, Delia antiqua, is a devastating pest of liliaceous crops and current control measures fail to avert pesticide residues, threats to agroecosystem, and costly …             The porin VDAC2 is the mitochondrial platform for Bax retrotranslocation ▶     Joachim Lauterwasser, Franziska Todt, Ralf M. Zerbes et al.     The pro-apoptotic Bcl-2 protein Bax can permeabilize the outer mitochondrial membrane and therefore commit human cells to apoptosis. Bax is regulated by constant translocation to …             PI3 Kinase Pathway and MET Inhibition is Efficacious in Malignant Pleural Mesothelioma ▶     Rajani Kanteti, Jacob J. Riehm, Immanuel Dhanasingh et al.     Malignant pleural mesothelioma (MPM) is an aggressive cancer that is commonly associated with prior asbestos exposure. Receptor tyrosine kinases (RTKs) such as MET and its …             Regulation of Mitoflash Biogenesis and Signaling by Mitochondrial Dynamics ▶     Wenwen Li, Tao Sun, Beibei Liu et al.     Mitochondria are highly dynamic organelles undergoing constant network reorganization and exhibiting stochastic signaling events in the form of mitochondrial flashes …             Crocetin exploits p53-induced death domain (PIDD) and FAS-associated death domain (FADD) proteins to induce apoptosis in colorectal cancer ▶     Pallab Ray, Deblina Guha, Juni Chakraborty et al.     Tumor suppressor p53 preserves the genomic integrity by restricting anomaly at the gene level. The hotspots for mutation in half of all colon cancers reside in p53. Hence, in a …             Altered Intranetwork and Internetwork Functional Connectivity in Type 2 Diabetes Mellitus With and Without Cognitive Impairment ▶     Shi-Qi Yang, Zhi-Peng Xu, Ying Xiong et al.     Type 2 diabetes mellitus (T2DM) is associated with cognitive impairment. We investigated whether alterations of intranetwork and internetwork functional connectivity with T2DM …             De novo sequencing, assembly and analysis of eight different transcriptomes from the Malayan pangolin ▶     Aini Mohamed Yusoff, Tze King Tan, Ranjeev Hari et al.     Pangolins are scale-covered mammals, containing eight endangered species. Maintaining pangolins in captivity is a significant challenge, in part because little is known about …             De novo Transcriptome Analysis Reveals Distinct Defense Mechanisms by Young and Mature Leaves of Hevea brasiliensis (Para Rubber Tree) ▶     Yongjun Fang, Hailiang Mei, Binhui Zhou et al.     Along with changes in morphology in the course of maturation, leaves of Hevea brasiliensis become more resistant to leaf diseases, including the South American Leaf Blight …             Characterization of a knock-in mouse model of the homozygous p.V37I variant in Gjb2 ▶     Ying Chen, Lingxiang Hu, Xueling Wang et al.     The homozygous p.V37I variant in GJB2 is prevalent in East and Southeast Asians and may lead to mild-to-moderate hearing loss with reduced penetrance. To investigate the …             Deoxyinosine triphosphate induces MLH1/PMS2- and p53-dependent cell growth arrest and DNA instability in mammalian cells ▶     Yasuto Yoneshima, Nona Abolhassani, Teruaki Iyama et al.     Deoxyinosine (dI) occurs in DNA either by oxidative deamination of a previously incorporated deoxyadenosine residue or by misincorporation of deoxyinosine triphosphate (dITP) …             Morphological change in cranial shape following the transition to agriculture across western Eurasia ▶     Olivia Cheronet, John A. Finarelli, Ron Pinhasi     The Neolithic transition brought about fundamental social, dietary and behavioural changes in human populations, which, in turn, impacted skeletal morphology. Crania are shaped …             Altered brain ion gradients following compensation for elevated CO2 are linked to behavioural alterations in a coral reef fish ▶     R. M. Heuer, M. J. Welch, J. L. Rummer et al.     Neurosensory and behavioural disruptions are some of the most consistently reported responses upon exposure to ocean acidification-relevant CO2 levels, especially in coral reef …             Crystal structures of archaeal 2-oxoacid:ferredoxin oxidoreductases from Sulfolobus tokodaii ▶     Zhen Yan, Akane Maruyama, Takatoshi Arakawa et al.     As the first three-dimensional structure of the two-subunit type 2-oxoacid:ferredoxin oxidoreductases (OFOR) from archaea, we solved the crystal structures of STK_23000/STK_22980 …             The yeast Aft2 transcription factor determines selenite toxicity by controlling the low affinity phosphate transport system ▶     María Pérez-Sampietro, Albert Serra-Cardona, David Canadell et al.     The yeast Saccharomyces cerevisiae is employed as a model to study the cellular mechanisms of toxicity and defense against selenite, the most frequent environmental selenium …             Patterns of contribution to citizen science biodiversity projects increase understanding of volunteers’ recording behaviour ▶     Elizabeth H. Boakes, Gianfranco Gliozzo, Valentine Seymour et al.     The often opportunistic nature of biological recording via citizen science leads to taxonomic, spatial and temporal biases which add uncertainty to biodiversity estimates. …             A polymorphic (GA/CT)n- SSR influences promoter activity of Tryptophan decarboxylase gene in Catharanthus roseus L. Don ▶     Santosh Kumar, Sabhyata Bhatia     Simple Sequence Repeats (SSRs) of polypurine-polypyrimidine type motifs occur very frequently in the 5′ flanks of genes in plants and have recently been implicated to have a role …             Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33 ▶     Iryna Saranchova, Jeffrey Han, Hui Huang et al.     A new paradigm for understanding immune-surveillance and immune escape in cancer is described here. Metastatic carcinomas express reduced levels of IL-33 and diminished levels of …             The analysis of heterotaxy patients reveals new loss-of-function variants of GRK5 ▶     Davor Lessel, Tariq Muhammad, Teresa Casar Tena et al.     G protein-coupled receptor kinase 5 (GRK5) is a regulator of cardiac performance and a potential therapeutic target in heart failure in the adult. Additionally, we have …             Concurrent micro-RNA mediated silencing of tick-borne flavivirus replication in tick vector and in the brain of vertebrate host ▶     Konstantin A. Tsetsarkin, Guangping Liu, Heather Kenney et al.     Tick-borne viruses include medically important zoonotic pathogens that can cause life-threatening diseases. Unlike mosquito-borne viruses, whose impact can be restrained via …             Utility of the dual-specificity protein kinase TTK as a therapeutic target for intrahepatic spread of liver cancer ▶     Ruoyu Miao, Yan Wu, Haohai Zhang et al.     Therapies for primary liver cancer, the third leading cause of cancer-related death worldwide, remain limited. Following multi-omics analysis (including whole genome and …             Pits, a protein interacting with Ttk69 and Sin3A, has links to histone deacetylation ▶     Gwo-Jen Liaw     Histone deacetylation plays an important role in transcriptional repression. Previous results showed that the genetic interaction between ttk and rpd3, which encodes a class I …             Cell wall degradation is required for normal starch mobilisation in barley endosperm ▶     Vasilios M. E. Andriotis, Martin Rejzek, Elaine Barclay et al.     Starch degradation in barley endosperm provides carbon for early seedling growth, but the control of this process is poorly understood. We investigated whether endosperm cell …             Vitamin B2 as a virulence factor in Pseudogymnoascus destructans skin infection ▶     Miroslav Flieger, Hana Bandouchova, Jan Cerny et al.     Pathogenic and non-pathogenic related microorganisms differ in secondary metabolite production. Here we show that riboflavin overproduction by a fungal pathogen and its …             Functional metabolic interactions of human neuron-astrocyte 3D in vitro networks ▶     Daniel Simão, Ana P. Terrasso, Ana P. Teixeira et al.     The generation of human neural tissue-like 3D structures holds great promise for disease modeling, drug discovery and regenerative medicine strategies. Promoting the …             Binding affinity of amyloid oligomers to cellular membranes is a generic indicator of cellular dysfunction in protein misfolding diseases ▶     Elisa Evangelisti, Roberta Cascella, Matteo Becatti et al.     The conversion of peptides or proteins from their soluble native states into intractable amyloid deposits is associated with a wide range of human disorders. Misfolded protein …             The multifaceted RisA regulon of Bordetella pertussis ▶     Loïc Coutte, Ludovic Huot, Rudy Antoine et al.     The whooping cough agent Bordetella pertussis regulates the production of its virulence factors by the BvgA/S system. Phosphorylated BvgA activates the virulence-activated genes (…             Early-life sexual segregation: ontogeny of isotopic niche differentiation in the Antarctic fur seal ▶     L. Kernaléguen, J. P. Y. Arnould, C. Guinet et al.     Investigating the ontogeny of niche differentiation enables to determine at which life-stages sexual segregation arises, providing insights into the main factors driving resource …             Inhibition of Drp1 mitochondrial translocation provides neural protection in dopaminergic system in a Parkinson’s disease model induced by MPTP ▶     Emily Filichia, Barry Hoffer, Xin Qi et al.     Accumulating evidence suggest mitochondria-mediated pathways play an important role in dopaminergic neuronal cell death in Parkinson’s disease (PD). Drp1, a key regulator of …             Augmented 3D super-resolution of fluorescence-free nanoparticles using enhanced dark-field illumination based on wavelength-modulation and a least-cubic algorithm ▶     Peng Zhang, Kyungsoo Kim, Seungah Lee et al.     Augmented three-dimensional (3D) subdiffraction-limited resolution of fluorescence-free single-nanoparticles was achieved with wavelength-dependent enhanced dark-field (EDF) …             Fluorescence imaging of chromosomal DNA using click chemistry ▶     Takumi Ishizuka, Hong Shan Liu, Kenichiro Ito et al.     Chromosome visualization is essential for chromosome analysis and genetic diagnostics. Here, we developed a click chemistry approach for multicolor imaging of chromosomal DNA …             HSCCC-based strategy for preparative separation of in vivo metabolites after administration of an herbal medicine: Saussurea laniceps, a case study ▶     Tao Yi, Lin Zhu, Guo-Yuan Zhu et al.     This paper reports a novel strategy based on high-speed counter-current chromatography (HSCCC) technique to separate in vivo metabolites from refined extract of urine after …             Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference ▶     Zhongping Xu, Jingwen Li, Xiaoping Guo et al.     Cottonseed oil is recognized as an important oil in food industry for its unique characters: low flavor reversion and the high level of antioxidants (VitaminE) as well as …             Trp53 deficient mice predisposed to preterm birth display region-specific lipid alterations at the embryo implantation site ▶     Ingela Lanekoff, Jeeyeon Cha, Jennifer E. Kyle et al.     Here we demonstrate that conditional deletion of mouse uterine Trp53 (p53d/d), molecularly linked to mTORC1 activation and causally linked to premature uterine senescence and …             Molecular organization, localization and orientation of antifungal antibiotic amphotericin B in a single lipid bilayer ▶     Wojciech Grudzinski, Joanna Sagan, Renata Welc et al.     Amphotericin B is a popular antifungal antibiotic, a gold standard in treatment of systemic mycotic infections, due to its high effectiveness. On the other hand, applicability of …             Lipid membrane-mediated attraction between curvature inducing objects ▶     Casper van der Wel, Afshin Vahid, Anđela Šarić et al.     The interplay of membrane proteins is vital for many biological processes, such as cellular transport, cell division, and signal transduction between nerve cells. Theoretical …             Real-time Functional Analysis of Inertial Microfluidic Devices via Spectral Domain Optical Coherence Tomography ▶     Biqin Dong, Siyu Chen, Fan Zhou et al.     We report the application of spectral-domain optical coherence tomography (SD-OCT) technology that enables real-time functional analysis of sorting microparticles and cells in an …             Quadrant Field Pupillometry Detects Melanopsin Dysfunction in Glaucoma Suspects and Early Glaucoma ▶     Prakash Adhikari, Andrew J. Zele, Ravi Thomas et al.     It is difficult to detect visual function deficits in patients at risk for glaucoma (glaucoma suspects) and at early disease stages with conventional ophthalmic tests such as …             Conformational Ensemble of hIAPP Dimer: Insight into the Molecular Mechanism by which a Green Tea Extract inhibits hIAPP Aggregation ▶     Yuxiang Mo, Jiangtao Lei, Yunxiang Sun et al.     Small oligomers formed early along human islet amyloid polypeptide (hIAPP) aggregation is responsible for the cell death in Type II diabetes. The epigallocatechin gallate (EGCG), …             4-Hydroxy-2-nonenal induces apoptosis by activating ERK1/2 signaling and depleting intracellular glutathione in intestinal epithelial cells ▶     Yun Ji, Zhaolai Dai, Guoyao Wu et al.     Excessive reactive oxygen species (ROS) induces oxidative damage to cellular constituents, ultimately leading to induction of apoptotic cell death and the pathogenesis of various …             Microwave ablation of hepatocellular carcinoma as first-line treatment: long term outcomes and prognostic factors in 221 patients ▶     Tao Wang, Xiao-Jie Lu, Jia-Chang Chi et al.     This retrospective study aimed at evaluating the long-term outcomes and prognostic factors of microwave ablation (MWA) as a first-line treatment for hepatocellular carcinoma …             Protective effects of sirtuin 3 in a murine model of sepsis-induced acute kidney injury ▶     Wen-Yu Zhao, Lei Zhang, Ming-Xing Sui et al.     Acute kidney injury (AKI) is a rapid loss of kidney function characterized by damage to renal tubular cells driven by mitochondrial dysregulation and oxidative stress. Here, we …             Targeting Motor End Plates for Delivery of Adenoviruses: An Approach to Maximize Uptake and Transduction of Spinal Cord Motor Neurons ▶     Andrew Paul Tosolini, Renée Morris     Gene therapy can take advantage of the skeletal muscles/motor neurons anatomical relationship to restrict gene expression to the spinal cord ventral horn. Furthermore, …             An Auditory BCI System for Assisting CRS-R Behavioral Assessment in Patients with Disorders of Consciousness ▶     Jun Xiao, Qiuyou Xie, Yanbin He et al.     The Coma Recovery Scale-Revised (CRS-R) is a consistent and sensitive behavioral assessment standard for disorders of consciousness (DOC) patients. However, the CRS-R has …             Cold tolerance is unaffected by oxygen availability despite changes in anaerobic metabolism ▶     Leigh Boardman, Jesper G. Sørensen, Vladimír Koštál et al.     Insect cold tolerance depends on their ability to withstand or repair perturbations in cellular homeostasis caused by low temperature stress. 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