Nature Biotechnology Contents: Volume 34 pp 213 - 356

If you are unable to see the message below, click here to view.

Advertisement Two NO COST pilot projects to be awarded. Register for our webcast on Building Better Therapeutics with Metabolomics and be eligible to submit your abstract for 1 of 2 50 sample projects using metabolomics.  Register for the webcast free online.   TABLE OF CONTENTS March 2016 Volume 34, Issue 3 In This Issue Editorial News Bioentrepreneur Opinion and Comment Features News and Views Research Careers and Recruitment Advertisement This special Focus highlights the unprecedented insights gained into the regulatory mechanisms underlying nuclear reprogramming, pluripotency and cell identity, and looks at the progress and challenges in using embryonic stem (ES) cells and iPSCs for therapeutic applications. Available free online. Produced with support from Thermo Fisher Scientific  Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement nature.com webcasts Join us for our upcoming webcast with live Q&A: COMBATING ANTIBIOTIC RESISTANCE Presented by BioPharma Dealmakers Date: Wednesday, March 9, 2016 Time: 8AM PST, 11AM EST, 4PM GMT, 5PM CET REGISTER FOR FREE Sponsored by: Antabio, Cempra, Helperby, Motif Bio, Polyphor     Advertisement Key Issues in Alzheimer's Disease- a community forum  Nature Neuroscience presents a forum on Alzheimer's Disease research. ACCESS NOW to view videos of our panel event at SfN 2015, read related articles for free, and post questions for our editors and featured experts. Produced with support from  Eli Lilly and Company and vTv Therapeutics     Nature Biotechnology 20th Anniversary Contents Editorial Features Patents   Focus blurb here In This Issue Top In this issue   ppvii - vii doi:10.1038/nbt.3518 Editorial Top Breaking out of the bubble   p213 doi:10.1038/nbt.3517 The biotech community has made remarkable progress over the past 20 years. Now it needs to break out of its bubble. News Top Immune profiling players shift gear to guide cancer drug development   pp215 - 216 Michael Eisenstein doi:10.1038/nbt0316-215 Drug developers refocus efforts on RAS   pp217 - 218 Cormac Sheridan doi:10.1038/nbt0316-217 Natural killer cells blaze into immuno-oncology   pp219 - 220 Ken Garber doi:10.1038/nbt0316-219 FDA head Califf spurned   p220 doi:10.1038/nbt0316-220a GM salmon shut out of US   p220 doi:10.1038/nbt0316-220b GM mosquitoes fire first salvo against Zika virus   pp221 - 222 Emily Waltz doi:10.1038/nbt0316-221 Bial incident raises FAAH suspicions   p223 Eva von Schaper doi:10.1038/nbt0316-223a Seed spy pleads guilty   p223 Emily Waltz doi:10.1038/nbt0316-223b Sanofi drops MannKind's inhaled insulin   p224 Mark Ratner doi:10.1038/nbt0316-224 Around the world in a month   p225 doi:10.1038/nbt0316-225 Advertisement Enhanced MALDI-TOF MS Performance and Flexibility  From a linear-only system to a unique QIT-TOF configuration to a high-resolution, high-energy TOF-TOF, Shimadzu's suite of MALDI mass spectrometers easily meets the needs of researchers with varying applications and budgets. Mine your data using our comprehensive software solutions. Learn more.   Bioentrepreneur Building a business Aligning needs   pp226 - 230 Dennis Ford and William Kohlbrenner doi:10.1038/nbt.3497 Opinion and Comment Top Correspondence Deciphering the EU clinical trials regulation   pp231 - 233 Mohamed Abou-El-Enein and Christian K Schneider doi:10.1038/nbt.3492 A call for industry to embrace green biopharma   pp234 - 235 Kristi L Budzinski, Stefan G Koenig, Deborah A O'Connor and Tse-Sung Wu doi:10.1038/nbt.3493 Marketplace response to GM animal products   pp236 - 238 Damien Mather, Rasmus Vikan and John Knight doi:10.1038/nbt.3494 Commentary Digital medicine's march on chronic disease   pp239 - 246 Joseph C Kvedar, Alexander L Fogel, Eric Elenko and Daphne Zohar doi:10.1038/nbt.3495 Digital medicine offers the possibility of continuous monitoring, behavior modification and personalized interventions at low cost, potentially easing the burden of chronic disease in cost-constrained healthcare systems. Features Top Estimating the biotech sector's contribution to the US economy   pp247 - 255 Robert Carlson doi:10.1038/nbt.3491 US biotech sector revenue is estimated to have grown on average >10% each year over the past decade[mdash]much faster than the rest of the economy. A more comprehensive assessment of biotech's economic contribution, however, will require improved data collection, classification and analysis. 20 years of Nature Biotechnology research tools   pp256 - 261 Anna Azvolinsky, Laura DeFrancesco, Emily Waltz and Sarah Webb doi:10.1038/nbt.3507 Authors of some of the most highly cited Nature Biotechnology papers that describe research tools discuss their work and challenges for their fields. 20 years of Nature Biotechnology biomedical research   pp262 - 266 Anna Azvolinsky, Charles Schmidt, Emily Waltz and Sarah Webb doi:10.1038/nbt.3509 Authors of some of the most highly cited Nature Biotechnology biomedical papers from the past 20 years discuss their work and challenges for their fields. 20 years of Nature Biotechnology bioengineering research   pp267 - 269 Laura DeFrancesco, Charles Schmidt and Emily Waltz doi:10.1038/nbt.3508 Authors of some of the most highly cited Nature Biotechnology nonbiomedical papers from the past 20 years discuss their work and challenges for their fields. Voices of biotech   pp270 - 275 Ido Amit, David Baker, Roger Barker, Bonnie Berger, Carolyn Bertozzi et al. doi:10.1038/nbt.3502 Nature Biotechnology asks a selection of researchers about the most exciting frontier in their field and the most needed technologies for advancing knowledge and applications. Community crystal gazing   pp276 - 283 Anu Acharya, Kate Bingham, Jay Bradner, Wylie Burke, R Alta Charo et al. doi:10.1038/nbt.3515 A selection of individuals from the biotech ecosystem give their views on the challenges facing the sector over the coming years. When biotech goes bad   pp284 - 291 John Hodgson doi:10.1038/nbt.3510 A look at 20 years of the dark side of biotech. Patents The changing life science patent landscape   pp292 - 294 Arti K Rai and Jacob S Sherkow doi:10.1038/nbt.3504 What have we learned from 20 tumultuous years of patent law in the life sciences? Is patenting likely to be as important for the industry in the future? Advertisement Roles for mesenchymal stem cells as medicinal signaling cells Understanding the in vivo identity and function of mesenchymal stem cells (MSCs) is vital to fully exploit their therapeutic potential. This poster summarizes current thinking regarding the role of MSCs in vivo and also describes how to isolate MSCs and grow them in vitro. Download the poster free online. Produced with support from:  STEMCELL Technologies   News and Views Top From clinical imaging to implantation of 3D printed tissues   pp295 - 296 E Thomas Pashuck and Molly Stevens doi:10.1038/nbt.3503 Mechanically stable bone, cartilage and muscle tissues are printed in custom shapes. See also: Research by Kang et al. Haplotypes drop by drop   pp296 - 298 Jacob O Kitzman doi:10.1038/nbt.3500 Short-read sequencing provides haplotype information when long DNA fragments are barcoded in microfluidic droplets. See also: Research by Zheng et al. Cas9 loosens its grip on off-target sites   pp298 - 299 Christopher E Nelson and Charles A Gersbach doi:10.1038/nbt.3501 Two new variants of the Cas9 nuclease have minimal activity at off-target DNA sites. Research Highlights   p299 doi:10.1038/nbt.3499 Biotechnology JOBS of the week Full Professor (W3) Synthetic Biotechnology Friedrich Schiller University Jena Postdoctoral Fellowships in Health Sciences - 45 positions University of Oslo Research Assistant in Gene Expression and Genome Stability The Babraham Institute Postdoc Positions Max Planck Institute for the Physics of Complex Systems Positions in Plant Biology National University of Singapore (NUS) More Science jobs from Research Top Analysis Fast search of thousands of short-read sequencing experiments   pp300 - 302 Brad Solomon and Carl Kingsford doi:10.1038/nbt.3442 A data indexing method enables fast and computationally efficient searching of short-read sequencing archives. Articles Haplotyping germline and cancer genomes with high-throughput linked-read sequencing   pp303 - 311 Grace X Y Zheng, Billy T Lau, Michael Schnall-Levin, Mirna Jarosz, John M Bell et al. doi:10.1038/nbt.3432 A microfluidics approach that links short sequence reads enables haplotype construction and complex variation identification from tiny amounts of input DNA. See also: News and Views by Kitzman A 3D bioprinting system to produce human-scale tissue constructs with structural integrity   pp312 - 319 Hyun-Wook Kang, Sang Jin Lee, In Kap Ko, Carlos Kengla, James J Yoo et al. doi:10.1038/nbt.3413 A new bioprinting system produces large tissue constructs with enough structural stability for surgical implantation. See also: News and Views by Pashuck & Stevens Minimally invasive endovascular stent-electrode array for high-fidelity, chronic recordings of cortical neural activity   pp320 - 327 Thomas J Oxley, Nicholas L Opie, Sam E John, Gil S Rind, Stephen M Ronayne et al. doi:10.1038/nbt.3428 Cortical activity can be monitored for 6 months or longer from within the brain vasculature using an endovascular stent-electrode array. Letters Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo    pp328 - 333 Hao Yin, Chun-Qing Song, Joseph R Dorkin, Lihua J Zhu, Yingxiang Li et al. doi:10.1038/nbt.3471 Cas9-mediated gene editing corrects hereditary tyrosinemia in a mouse model. A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice   pp334 - 338 Yang Yang, Lili Wang, Peter Bell, Deirdre McMenamin, Zhenning He et al. doi:10.1038/nbt.3469 In vivo delivery of CRISPR-Cas9 corrects mutation in newborn mouse liver. Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA   pp339 - 344 Christopher D Richardson, Graham J Ray, Mark A DeWitt, Gemma L Curie and Jacob E Corn doi:10.1038/nbt.3481 The efficiency of homology-directed genome editing with CRISPR-Cas9 is boosted through improved design of donor DNA. Combinatorial hydrogel library enables identification of materials that mitigate the foreign body response in primates   pp345 - 352 Arturo J Vegas, Omid Veiseh, Joshua C Doloff, Minglin Ma, Hok Hei Tam et al. doi:10.1038/nbt.3462 In vivo screening of a large combinatorial library of alginates identifies materials that elicit a substantially reduced foreign body response. Careers and Recruitment Top Towards sustaining a culture of mental health and wellness for trainees in the biosciences   pp353 - 355 Jessica W Tsai and Fanuel Muindi doi:10.1038/nbt.3490 Addressing the mental health of graduate and postdoctoral trainees in the biosciences will substantially benefit the scientific community at large. People People   p356 doi:10.1038/nbt.3512 Advertisement An open access, online-only, multidisciplinary research journal dedicated to publishing the most important scientific advances in the life sciences, physical sciences, and engineering fields that are facilitated by spaceflight and analogue platforms. Explore the benefits of submitting your next research article.   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/myaccount (You will need to log in to be recognised as a nature.com registrant) For further technical assistance, please contact our registration department For print subscription enquiries, please contact our subscription department For other enquiries, please contact our customer feedback department Nature Publishing Group | One New York Plaza, Suite 4500 | New York | NY 10004-1562 | USA Nature Publishing Group's worldwide offices: London - Paris - Munich - New Delhi - Tokyo - Melbourne San Diego - San Francisco - Washington - New York - Boston Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at Brunel Road, Houndmills, Basingstoke, Hampshire RG21 6XS. © 2016 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.