Nature Structural & Molecular Biology Contents: 2015 Volume #22 pp 941-1033

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TABLE OF CONTENTS December 2015 Volume 22, Issue 12 Correspondence News and Views Perspective Articles Brief Communication Resource Subscribe   Facebook   RSS   Recommend to library   Twitter   Correspondence Top Ligand occupancy in crystal structure of β1-adrenergic G protein-coupled receptor   pp941 - 942 Andrew G W Leslie, Tony Warne and Christopher G Tate doi:10.1038/nsmb.3130 Ligand occupancy in crystal structure of β1-adrenergic G protein-coupled receptor   p942 Jianyun Huang, Shuai Chen, J Jillian Zhang and Xin-Yun Huang doi:10.1038/nsmb.3131 News and Views Top Q&A: repeat-containing proteins   pp943 - 945 Regina M Murphy doi:10.1038/nsmb.3135 Polyglutamine (polyGln) expansions induce protein misfolding and aggregation into fibrillar deposits, which are believed to underlie forms of neurodegeneration through a mechanism involving gain of toxic function. Polyalalanine expansions are now shown to differ from polyGln in kinetics of aggregation, morphology of aggregates and mechanism of toxicity. See also: Article by Polling et al. Src defines a new pool of EGFR substrates   pp945 - 947 Nicole Michael and Natalia Jura doi:10.1038/nsmb.3137 The mechanisms guiding the substrate specificity of kinases are still poorly understood. Two recent reports provide further insights into how the epidermal growth factor receptor (EGFR) tyrosine kinase recognizes targets by identifying a new consensus motif that requires a Src-mediated priming phosphorylation. See also: Article by Begley et al. Replication- and transcription-independent histone exchange in oocytes   p947 Katrina Woolcock doi:10.1038/nsmb.3143 Structural & Molecular Biology JOBS of the week Independent Group Leader Ludwig-Maximilians-Universität München, Gene Center Exciting PhD Positions in Modern Biology VBC PhD Programme Research Assistant / Associate in Structural Biology (Fixed Term) University of Cambridge Postdoc Position in Single-Molecule Biophysics Albert Einstein College of Medicine, Inc. Tenure-track Assistant Professor in Molecular Pharmacology Université de Sherbrooke More Science jobs from Perspective Top Probing molecular choreography through single-molecule biochemistry   pp948 - 952 Antoine M van Oijen and Nicholas E Dixon doi:10.1038/nsmb.3119 In this Perspective, the authors discuss how recent innovations in single-molecule fluorescence approaches now permit protein dynamics to be monitored in increasingly complex biological systems and cellular environments. Advertisement Established as a sister journal of Cell Research, Cell Discovery is a new fully open access international journal publishing original articles and reviews on results of significance and originality in all areas of molecular and cell biology. Submit your manuscript   Articles Top Structure of full-length human anti-PD1 therapeutic IgG4 antibody pembrolizumab   pp953 - 958 Giovanna Scapin, Xiaoyu Yang, Winifred W Prosise, Mark McCoy, Paul Reichert, Jennifer M Johnston, Ramesh S Kashi & Corey Strickland doi:10.1038/nsmb.3129 IgG4 antibodies can exchange Fab arms and show different affinities for Fc receptors than do other IgG subclasses. The structure of full-length pembrolizumab, a human IgG4 approved to treat advanced melanoma, provides a framework to understand IgG4's properties. A new vertebrate SUMO enzyme family reveals insights into SUMO-chain assembly   pp959 - 967 Nathalie Eisenhardt, Viduth K Chaugule, Stefanie Koidl, Mathias Droescher, Esen Dogan, Jan Rettich, Päivi Sutinen, Susumu Y Imanishi, Kay Hofmann, Jorma J Palvimo & Andrea Pichler doi:10.1038/nsmb.3114 The SUMO E3 ligase ZNF451 is a representative member of a new class of SUMO enzymes that execute catalysis via tandem SUMO-interaction motifs, thus allowing efficient SUMO-chain formation. Structural basis for catalytic activation by the human ZNF451 SUMO E3 ligase   pp968 - 975 Laurent Cappadocia, Andrea Pichler and Christopher D Lima doi:10.1038/nsmb.3116 Structural and biochemical analyses establish the catalytic mechanism of ZNF451, a new class of E3 ligases. The architecture of a eukaryotic replisome   pp976 - 982 Jingchuan Sun, Yi Shi, Roxana E Georgescu, Zuanning Yuan, Brian T Chait, Huilin Li & Michael E O'Donnell doi:10.1038/nsmb.3113 Single-particle electron microscopy reconstruction of the budding yeast replisome locates leading-strand Pol ε and lagging-strand Pol α on opposite sides of the CMG helicase and suggests a new DNA path through the complex during replication. EGF-receptor specificity for phosphotyrosine-primed substrates provides signal integration with Src   pp983 - 990 Michael J Begley, Cai-hong Yun, Christina A Gewinner, John M Asara, Jared L Johnson, Anthony J Coyle, Michael J Eck, Irina Apostolou & Lewis C Cantley doi:10.1038/nsmb.3117 Preference of EGFR for substrates that are primed by prior phosphorylation provides the molecular explanation for integration of Src and EGFR signaling via Src-mediated phosphorylation of the adaptor protein Shc1. See also: News and Views by Michael & Jura The role of lipids in mechanosensation   pp991 - 998 Christos Pliotas, A Caroline E Dahl, Tim Rasmussen, Kozhinjampara R Mahendran, Terry K Smith, Phedra Marius, Joseph Gault, Thandiwe Banda, Akiko Rasmussen, Samantha Miller, Carol V Robinson, Hagan Bayley, Mark S P Sansom, Ian R Booth & James H Naismith doi:10.1038/nsmb.3120 Crystallographic, biophysical and in silico analyses indicate that the conformational state of the mechanosensitive channel MscS is determined by the reorganization, due to changes in membrane tension, of the lipids within and around the protein. R loops regulate promoter-proximal chromatin architecture and cellular differentiation   pp999 - 1007 Poshen B Chen, Hsiuyi V Chen, Diwash Acharya, Oliver J Rando and Thomas G Fazzio doi:10.1038/nsmb.3122 New data show that R loops differentially modulate binding of chromatin remodelers Tip60-p400 and PRC2 at coding and noncoding gene promoters of mouse ESCs and thereby control transcription and cellular differentiation. Polyalanine expansions drive a shift into α-helical clusters without amyloid-fibril formation   pp1008 - 1015 Saskia Polling, Angelique R Ormsby, Rebecca J Wood, Kristie Lee, Cheryl Shoubridge, James N Hughes, Paul Q Thomas, Michael D W Griffin, Andrew F Hill, Quill Bowden, Till Böcking & Danny M Hatters doi:10.1038/nsmb.3127 In vitro and in vivo analyses show that the aggregation mechanism of polyalanine expansions is based on assembly into α-helical clusters with diverse oligomeric species, in contrast to that of polyglutamine expansions, which form amyloid fibrils. See also: News and Views by Murphy Structural characterization of human heparanase reveals insights into substrate recognition   pp1016 - 1022 Liang Wu, Cristina M Viola, Andrzej M Brzozowski and Gideon J Davies doi:10.1038/nsmb.3136 Crystal structures of human heparanase in its apo form and bound to oligosaccharide substrates offer insights into the mechanism of substrate recognition and catalysis. Brief Communication Top The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex   pp1023 - 1026 Bingbing Wan, Ting Tang, Heather Upton, Jin Shuai, Yuanzhe Zhou, Song Li, Juan Chen, Joseph S Brunzelle, Zhixiong Zeng, Kathleen Collins, Jian Wu & Ming Lei doi:10.1038/nsmb.3126 New crystal structures and supporting functional assays identify the p19 and p45 subunits of Tetrahymena telomerase as homologs of the Stn1 and Ten1 subunits of the CST complex, which coordinates telomere replication. Resource Top Conserved mRNA-binding proteomes in eukaryotic organisms   pp1027 - 1033 Ana M Matiá-Gonzalez, Emma E Laing and André P Gerber doi:10.1038/nsmb.3128 Comprehensive identification of mRNA-binding proteins in S. cerevisiae and C. elegans reveals their evolutionary conservation; strikingly, most components of the glycolytic pathway and proteasome are detected, thus possibly indicating an ancient mechanism for metabolic control. Top Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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