Laboratory Investigation - Table of Contents alert Volume 95 Issue 6

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TABLE OF CONTENTS Volume 95, Issue 6 (June 2015) In this issue Inside the USCAP Journals Pathobiology in Focus Research Articles Corrigenda Also new AOP Sign up for e-alerts Recommend to your library Web feed Subscribe Inside the USCAP Journals Top Inside the USCAP Journals 2015 95: 574-575; 10.1038/labinvest.2015.65 Full Text Pathobiology in Focus Top Chronic traumatic encephalopathy: A paradigm in search of evidence? This editorial review highlights the evolving paradigm surrounding chronic traumatic encephalopathy (CTE), and gaps in knowledge which require further study. An in-depth understanding of CTE by the pathology community is critical to scientific investigation, as current concepts surrounding this entity have broad implications in society, from athletic participation to armed conflict. Rudy J Castellani 2015 95: 576-584; advance online publication, April 13, 2015; 10.1038/labinvest.2015.54 Abstract | Full Text Research Articles Top BREAST, SKIN, SOFT TISSUE AND BONE Cavin-1 and Caveolin-1 are both required to support cell proliferation, migration and anchorage-independent cell growth in rhabdomyosarcoma In this paper, the authors employed human rhabdomyosarcoma (RMS) cell lines as well as mouse models that faithfully recapitulate the onset and progression of this soft tissue sarcoma in order to determine mechanisms of tumor growth and invasion. They found that cavin-1, which forms plasma membrane complexes with caveolin-1 to form caveolae, contributes to sustain proliferation, migration and anchorage-independent growth of RMS cells. Fiorella Faggi, Nicola Chiarelli, Marina Colombi, Stefania Mitola, Roberto Ronca, Luca Madaro, Marina Bouche, Pietro L Poliani, Marika Vezzoli, Francesca Longhena, Eugenio Monti, Barbara Salani, Davide Maggi, Charles Keller and Alessandro Fanzani 2015 95: 585-602; advance online publication, March 30, 2015; 10.1038/labinvest.2015.45 Abstract | Full Text RNA sequencing of sarcomas with simple karyotypes: identification and enrichment of fusion transcripts With the aim of identifying new gene fusions, eight sarcomas with simple karyotypes were subjected to RNA sequencing. By using three different algorithms, three novel and two previously known fusions could be identified. However, only two of the confirmed fusions were reported by more than one algorithm, emphasizing the importance of using multiple tools to analyze RNA sequencing data. Jakob Hofvander, Johnbosco Tayebwa, Jenny Nilsson, Linda Magnusson, Otte Brosjö, Olle Larsson, Fredrik Vult von Steyern, Henryk A Domanski, Nils Mandahl and Fredrik Mertens 2015 95: 603-609; advance online publication, April 13, 2015; 10.1038/labinvest.2015.50 Abstract | Full Text ANGIOGENESIS, CARDIOVASCULAR AND PULMONARY SYSTEMS Gammaherpesvirus infection modulates the temporal and spatial expression of SCGB1A1 (CCSP) and BPIFA1 (SPLUNC1) in the respiratory tract OPEN Scgb1a1 and Bpifa1 encode potential respiratory innate defense proteins. Employing a new, more authentic mouse model of γ-herpesvirus infection, the authors show that the M3 chemokine-binding protein contributes significantly to viral pathogenesis by regulating levels of both SCGB1A1 and BPIFA1. They also reveal that BPIFA1 expression occurs in bronchiolar exocrine cells along with SCGB1A1, but that BPIFA1 is stored within granules. Gail H Leeming, Anja Kipar, David J Hughes, Lynne Bingle, Elaine Bennett, Nathifa A Moyo, Ralph A Tripp, Alison L Bigley, Colin D Bingle, Jeffery T Sample and James P Stewart 2015 95: 610-624; advance online publication, December 22, 2014; 10.1038/labinvest.2014.162 Abstract | Full Text Decreased proteasomal function accelerates cigarette smoke-induced pulmonary emphysema in mice This paper reveals that the pathogenesis of chronic obstructive pulmonary disease (COPD) is related to oxidative stress-induced apoptosis in alveolar cells. Transgenic (Tg) mice with decreased proteasomal chymotrypsin-like activity experience accelerated development of cigarette smoke (CS)-induced pulmonary emphysema. Morphologically, CS-exposed Tg mice show remarkable air space enlargement, increased foci of inflammation and apoptotic cells in the alveolar walls of the affected lungs. Yosuke Yamada, Utano Tomaru, Akihiro Ishizu, Tomoki Ito, Takayuki Kiuchi, Ayako Ono, Syota Miyajima, Katsura Nagai, Tsunehito Higashi, Yoshihiro Matsuno, Hirotoshi Dosaka-Akita, Masaharu Nishimura, Soichi Miwa and Masanori Kasahara 2015 95: 625-634; advance online publication, April 27, 2015; 10.1038/labinvest.2015.43 Abstract | Full Text High-mobility group box 1 accelerates lipopolysaccharide-induced lung fibroblast proliferation in vitro: involvement of the NF-κB signaling pathway The mechanism underlying lipopolysaccharide (LPS)-induced aberrant proliferation of lung fibroblasts in Gram-negative bacilli-associated pulmonary fibrosis is examined in this study. The authors show that LPS-induced lung fibroblast secretion of high mobility group box 1 protein augments the pro-proliferative effects of LPS, and may exacerbate pulmonary fibrosis through the activation of the toll-like receptor 4/NF-κβ signaling pathway and its downstream targets. Wen Li, Qiaoyi Xu, Yuxiao Deng, Zhongwei Yang, Shunpeng Xing, Xianyuan Zhao, Ping Zhu, Xiangrui Wang, Zhengyu He and Yuan Gao 2015 95: 635-647; advance online publication, April 13, 2015; 10.1038/labinvest.2015.44 Abstract | Full Text ORAL AND GASTROINTESTINAL SYSTEMS Triptolide abrogates growth of colon cancer and induces cell cycle arrest by inhibiting transcriptional activation of E2F In this paper, the authors demonstrate that minnelide, a water-soluble analog of triptolide, is effective against colon cancer in multiple animal models of colon cancer. Mechanistically, they show that at low concentrations, triptolide induces apoptotic cell death but at higher concentrations it induces G1 cell cycle arrest by inhibiting transcriptional activation of E2F1 through modulation of events downstream of DNA binding. Amanda R Oliveira, Georg Beyer, Rohit Chugh, Steven J Skube, Kaustav Majumder, Sulagna Banerjee, Veena Sangwan, Lihua Li, Rajinder K Dawra, Subbaya Subramanian, Ashok K Saluja and Vikas Dudeja 2015 95: 648-659; advance online publication, April 20, 2015; 10.1038/labinvest.2015.46 Abstract | Full Text Osteopontin depletion decreases inflammation and gastric epithelial proliferation during Helicobacter pylori infection in mice The functional roles of osteopontin (OPN) using a mouse model of Helicobacter pylori (HP)-induced gastritis were investigated in this study. OPN exerts a considerable influence on HP-induced gastritis by contributing the production of cytokines and macrophage infiltration. Moreover, OPN-mediated activation of the MAP kinase pathway in gastric epithelial cells might contribute to epithelial changes following HP infection. Jun Won Park, Su Hyung Lee, Du Min Go, Hark Kyun Kim, Hyo-Jung Kwon and Dae-Yong Kim 2015 95: 660-671; advance online publication, April 13, 2015; 10.1038/labinvest.2015.47 Abstract | Full Text IMMUNE SYSTEM AND STEM CELLS Phenotypic diversity of patient-derived melanoma populations in stem cell medium Culture of patient-derived melanoma cells in stem cell media has been employed as a method to study melanoma biology, as the properties of parent melanomas are well preserved in patient-derived melanospheres. However, during prolonged culturing, when melanospheres are replaced by cell aggregates, the original heterogeneity is substantially lost. This should be considered when aggregates instead of melanospheres are used to determine melanoma properties and cellular responses to drugs. Malgorzata Sztiller-Sikorska, Mariusz L Hartman, Beata Talar, Justyna Jakubowska, Izabela Zalesna and Malgorzata Czyz 2015 95: 672-683; advance online publication, April 13, 2015; 10.1038/labinvest.2015.48 Abstract | Full Text Development and characterization of human-induced pluripotent stem cell-derived cholangiocytes This study demonstrates a novel approach to differentiate induced pluripotent stem cells into cholangiocytes, the epithelial cells that line biliary tree. After extensive characterization of the cells, the study shows that stem cell-derived cholangiocytes can engraft within liver in vivo after retrograde intra-biliary infusion. This work represents a platform for disease modelling, pharmacologic testing, and individualized regenerative therapies for the cholangiopathies. Thiago M De Assuncao, Yan Sun, Nidhi Jalan-Sakrikar, Mary C Drinane, Bing Q Huang, Ying Li, Jaime I Davila, Ruisi Wang, Steven P O'Hara, Gwen A Lomberk, Raul A Urrutia, Yasuhiro Ikeda and Robert C Huebert 2015 95: 684-696; advance online publication, April 13, 2015; 10.1038/labinvest.2015.51 Abstract | Full Text Corrigenda Top Serum amyloid A and inflammation in diabetic kidney disease and podocytes Robert J Anderberg, Rick L Meek, Kelly L Hudkins, Sheryl K Cooney, Charles E Alpers, Renee C Leboeuf and Katherine R Tuttle 2015 95: 697; 10.1038/labinvest.2015.38 Full Text Classification of malignant and benign tumors of the lung by infrared spectral histopathology (SHP) Ali Akalin, Xinying Mu, Mark A Kon, Ayşegül Ergin, Stan H Remiszewski, Clay M Thompson, Dan J Raz and Max Diem 2015 95: 697; 10.1038/labinvest.2015.55 Full Text Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. 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