The EMBO Journal Table of Contents for 12 March 2015; Vol. 34, No. 6

12 March 2015 | Volume 34, Number 6 Submit Table of Contents Have you seen? Reviews Articles Erratum Volume 34, Number 6 Have you seen? Prmt5: a guardian of the germline protects future generations Protecting the genetic integrity of the germline is of outmost importance. Two novel studies establish multiple roles of PRMT5 in safe‐guarding genomic integrity of PGCs, beyond earlier proposed roles in the induction of germline fate. Rebecca V Berrens and Wolf Reik Published online 16.02.2015 Creating cellular diversity through transcription factor competition Embryonic blood cell specification is studied intensely, given strong therapeutic interest. A few recent studies offer new insights into functional competition of key haematopoietic transcription factors, revealing a general framework for transcription factor‐mediated cell fate specification. Berthold Göttgens Published online 13.02.2015 Reviews Focus: Induced Pluripotency & Cellular Reprogramming: Transcription factor‐mediated reprogramming toward hematopoietic stem cells Rossi and Ebina summarize recent insights into transcriptional (re‐)programming of blood cells and highlight most promising routes for the generation of transplantable hematopoietic stem cells. Wataru Ebina and Derrick J Rossi Published online 20.02.2015 Focus: Induced Pluripotency & Cellular Reprogramming: Programming and reprogramming a human heart cell Kenneth Chien and colleagues review molecular principles of cardiogenesis, which hold great promise for regenerative “programming” or “reprogramming” of human heart conditions. Makoto Sahara, Federica Santoro, and Kenneth R Chien Published online 20.02.2015 Focus: Induced Pluripotency & Cellular Reprogramming: Reprogramming of human cancer cells to pluripotency for models of cancer progression Zaret & Kim feature recent work on induced reprogramming of cancer cells that may accelerate genuine modeling and new insights into human malignancies. Jungsun Kim and Kenneth S Zaret Published online 20.02.2015 Articles The Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice Conditional deletion of Prmt5 in the mouse germline rules out anticipated roles in germline specification. Instead, Prmt5 controls ground state pluripotency in primordial cells by virtue of RNA splicing. Ziwei Li, Juehua Yu, Linzi Hosohama, Kevin Nee, Sofia Gkountela, Sonal Chaudhari, Ashley A Cass, Xinshu Xiao, and Amander T Clark Published online 17.12.2014 Scl binds to primed enhancers in mesoderm to regulate hematopoietic and cardiac fate divergence Previous work had revealed that Scl promotes hemogenic competence while repressing cardiac fate. Comprehensive genome‐scale data presented here establish Scl‐mediated ‘enhancer decommissioning’ as underlying molecular mechanism. Tõnis Org, Dan Duan, Roberto Ferrari, Amelie Montel‐Hagen, Ben Van Handel, Marc A Kerényi, Rajkumar Sasidharan, Liudmilla Rubbi, Yuko Fujiwara, Matteo Pellegrini, Stuart H Orkin, Siavash K Kurdistani, and Hanna KA Mikkola Published online 06.01.2015 Compartment‐specific aggregases direct distinct nuclear and cytoplasmic aggregate deposition ‘Juxtanuclear’ deposits of misfolded protein in fact reside in a new intranuclear quality control compartment (INQ). INQ formation is dependent on Btn2, while cytosolic protein deposition is mediated by the Hsp42 aggregase. Stephanie BM Miller, Chi‐Ting Ho, Juliane Winkler, Maria Khokhrina, Annett Neuner, Mohamed YH Mohamed, D Lys Guilbride, Karsten Richter, Michael Lisby, Elmar Schiebel, Axel Mogk, and Bernd Bukau Published online 11.02.2015 Space exploration by dendritic cells requires maintenance of myosin II activity by IP3 receptor 1 The IP3 receptor IP3R1 controls the ability of immature dendritic cells to explore their environment by regulating calcium release from the endoplasmic reticulum and by reinforcing the activity of the myosin light chain (MLC) kinase. Paola Solanes, Mélina L Heuzé, Mathieu Maurin, Marine Bretou, Franziska Lautenschlaeger, Paolo Maiuri, Emmanuel Terriac, Maria‐Isabel Thoulouze, Pierre Launay, Matthieu Piel, Pablo Vargas, and Ana‐Maria Lennon‐Duménil Published online 30.01.2015 TFG clusters COPII‐coated transport carriers and promotes early secretory pathway organization TFG forms cup‐like octameric structures and acts as a meshwork between the ER and ER‐Golgi intermediate compartment (ERGIC) for localized crowding of COPII‐coated carriers and efficient cargo transport in the cell. Adam Johnson, Nilakshee Bhattacharya, Michael Hanna, Janice G Pennington, Amber L Schuh, Lei Wang, Marisa S Otegui, Scott M Stagg, and Anjon Audhya Published online 13.01.2015 Erratum BRCA1 and CtIP promote alternative non‐homologous end‐joining at uncapped telomeres Sophie Badie, Ana Rita Carlos, Cecilia Folio, Keiji Okamoto, Peter Bouwman, Jos Jonkers, and Madalena Tarsounas Published online 11.03.2015   Unsubscribe from or edit your subscription for this service. Or by mail: Customer Service * 425 Broadway St * Redwood City, CA 94063 * U.S.A. Copyright © 2015 by the European Molecular Biology Organisation.