Molecular Systems Biology - New articles published - 1 Nov 2014

October 2014 | Volume 10, Number 10 Submit Table of Contents Articles Reports News & Views Articles Volume 10, Number 10 Articles Allelic expression mapping across cellular lineages to establish impact of non‐coding SNPs A comprehensive analysis cis‐variation in four cell‐populations uncovers a new‐class of cis‐regulatory SNPs associated with repressor activity. Global analysis of the role of key regulators is achieved by combining allelic expression read‐outs with targeted perturbation of transcription factors. Veronique Adoue, Alicia Schiavi, Nicholas Light, Jonas Carlsson Almlöf, Per Lundmark, Bing Ge, Tony Kwan, Maxime Caron, Lars Rönnblom, Chuan Wang, Shu‐Huang Chen, Alison H Goodall, Francois Cambien, Panos Deloukas, Willem H Ouwehand, Ann‐Christine Syvänen, and Tomi Pastinen Published online 16.10.2014 Reports Constant rate of p53 tetramerization in response to DNA damage controls the p53 response Quantification of the dynamics of p53 tetramers in single cells using a fluorescent protein‐fragment complementation assay reveals that, while total p53 increases proportionally to the DNA damage strength, p53 tetramers are formed at a constant rate. Giorgio Gaglia and Galit Lahav Published online 24.10.2014 News & Views Efficient sample processing for proteomics applications—Are we there yet? A new proteomic sample preparation protocol by Krijgsveld and colleagues (Hughes et al, 2014) enables all sample processing steps to be carried out in a single tube to minimize sample losses, thereby enhancing sensitivity, throughput, and scalability of proteomics analyses. Evgeny Kanshin and Pierre Thibault Published online 30.10.2014 Articles Ultrasensitive proteome analysis using paramagnetic bead technology A new proteomic sample preparation protocol allows fast, efficient and ultra‐sensitive analyses. The method is illustrated by profiling proteomes from sub‐microgram amounts of material, including the first proteome screen of Drosophila development at a single‐embryo resolution. Christopher S Hughes, Sophia Foehr, David A Garfield, Eileen E Furlong, Lars M Steinmetz, and Jeroen Krijgsveld Published online 30.10.2014 An organ boundary‐enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation The leaf boundary regions separate differentiated organs from undifferentiated stem cells in plants. The gene regulatory network of boundary cells was mapped by combining cell type‐specific genome expression analysis with genomewide yeast one‐hybrid screening. Caihuan Tian, Xiaoni Zhang, Jun He, Haopeng Yu, Ying Wang, Bihai Shi, Yingying Han, Guoxun Wang, Xiaoming Feng, Cui Zhang, Jin Wang, Jiyan Qi, Rong Yu, and Yuling Jiao Published online 30.10.2014   Unsubscribe from or edit your subscription for this service. Or by mail: Customer Service * 425 Broadway St * Redwood City, CA 94063 * U.S.A. Copyright © 2014 by the European Molecular Biology Organisation.