The EMBO Journal Table of Contents for 18 March 2014; Vol. 33, No. 6

18 March 2014 | Volume 33, Number 6 Submit Table of Contents Have you seen? Correspondence Articles Resource Volume 33, Number 6 Have you seen? Sensing the hybrid—a novel PAMP for TLR9 RNA:DNA hybrids represent a new type of pathogen‐associated molecular pattern, which signals via TLR9 to regulate cytokine and IFN responses in dendritic cells. Søren B Jensen and Søren R Paludan Published online 12.02.2014 Arrestin makes T cells stop and become active New work describes unsuspected functions of β‐arrestin‐1 at the immunological synapse, involving an unusual arrestin activation mechanism. Vsevolod V Gurevich and Eugenia V Gurevich Published online 06.02.2014 Sex hormone drives blood stem cell reproduction Although stem cells are normally under paracrine control, self‐renewal of hematopoietic stem cells in females is influenced by long‐range signals via estrogen. Vincenzo Calvanese, Lydia K Lee, and Hanna K A Mikkola Published online 20.02.2014 Correspondence Antisense piRNA amplification, but not piRNA production or nuage assembly, requires the Tudor‐domain protein Qin Zhao Zhang, Birgit S Koppetsch, Jie Wang, Cindy Tipping, Zhiping Weng, William E Theurkauf, and Phillip D Zamore Published online 20.03.2014 Response to Zhang et al Amit Anand and Toshie Kai Published online 20.03.2014 Articles RNA:DNA hybrids are a novel molecular pattern sensed by TLR9 TLR9, classically known as cytokine‐inducing sensor of bacterial DNA, gains an additional role in detecting RNA:DNA hybrids containing virus‐derived sequences, making them a novel type of pathogen‐associated molecular pattern. Rachel E Rigby, Lauren M Webb, Karen J Mackenzie, Yue Li, Andrea Leitch, Martin A M Reijns, Rachel J Lundie, Ailsa Revuelta, Donald J Davidson, Sandra Diebold, Yorgo Modis, Andrew S MacDonald, and Andrew P Jackson Published online 10.02.2014 β‐arrestin‐1 mediates the TCR‐triggered re‐routing of distal receptors to the immunological synapse by a PKC‐mediated mechanism Activated T‐cell receptors (TCRs) employ β‐arrestin‐mediated internalization to downregulate migratory chemokine receptors, but surprisingly also to recruit unliganded peripheral TCRs to the immunological synapse. Elena Fernández‐Arenas, Enrique Calleja, Nadia Martínez‐Martín, Severine I Gharbi, Rosana Navajas, Noel García‐Medel, Petronila Penela, Antonio Alcamí, Federico Mayor, Jr, Juan P Albar, and Balbino Alarcón Published online 06.02.2014 Stress‐induced OMA1 activation and autocatalytic turnover regulate OPA1‐dependent mitochondrial dynamics A sensor domain for mitochondrial stress and a novel self‐degradation mechanism enables the mitochondrial protease OMA1 to reversibly control mitochondrial fragmentation in response to various cellular stresses. Michael J Baker, Philipp A Lampe, Diana Stojanovski, Anne Korwitz, Ruchika Anand, Takashi Tatsuta, and Thomas Langer Published online 18.02.2014 Structural and mechanistic insights into MICU1 regulation of mitochondrial calcium uptake Crystal structures of Ca2+‐free and Ca2+‐bound human MICU1 offer first and significant structural insights into the mitochondrial calcium transport machinery, which was only very recently described at the molecular level. Lele Wang, Xue Yang, Siwei Li, Zheng Wang, Yu Liu, Jianrong Feng, Yushan Zhu, and Yuequan Shen Published online 10.02.2014 Prereplicative complexes assembled in vitro support origin‐dependent and independent DNA replication Plasmids licensed with purified replication initiation proteins can be successfully replicated in extracts from firing factor‐overexpressing budding yeast cells, validating Mcm2‐7 double hexamers as functional replication precursors. Kin Fan On, Fabienne Beuron, David Frith, Ambrosius P Snijders, Edward P Morris, and John F X Diffley Published online 24.02.2014 Origin plasticity during budding yeast DNA replication in vitro Reconstituted pre‐replication complexes support cell‐free replication with no strict initiation sequence requirement, hinting to an epigenetic component in replication origin specification even in budding yeast. Julien Gros, Sujan Devbhandari, and Dirk Remus Published online 24.02.2014 Ibf1 and Ibf2 are novel CP190‐interacting proteins required for insulator function Two new factors join the ranks of fly insulator proteins that cooperate with CP190, helping to understand CP190 action at genomic binding sites not shared with other known insulator proteins. Sergi Cuartero, Ujué Fresán, Oscar Reina, Evarist Planet, and M Lluisa Espinàs Published online 06.02.2014 Resource Proteomics of a fuzzy organelle: interphase chromatin A combination of biochemical and biological classifiers results in assignment of a protein ‘interphase chromatin probability’ (ICP), making an organelle whose dynamic composition defies rigorous black‐and‐white delineation amenable to quantitative proteomics. Georg Kustatscher, Nadia Hégarat, Karen L H Wills, Cristina Furlan, Jimi‐Carlo Bukowski‐Wills, Helfrid Hochegger, and Juri Rappsilber Published online 16.02.2014   Unsubscribe from or edit your subscription for this service. Or by mail: Customer Service * 425 Broadway St * Redwood City, CA 94063 * U.S.A. Copyright © 2014 by the European Molecular Biology Organisation.