Nature Biotechnology Contents: Volume 31 pp 661 - 768

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Produced with support from:     Editorial Top Open to interpretation   p661 doi:10.1038/nbt.2665 An international alliance to enable secure sharing of human genomic and clinical data merits both broad support and financial backing from the global research and clinical communities. News Top Myriad decision aftershocks ripple through biotech   pp663 - 665 Mark Ratner doi:10.1038/nbt0813-663 UCLA and GSK reconcile   p664 Nuala Moran doi:10.1038/nbt0813-664 BARDA to pick and choose next-generation antibiotics   p665 Gunjan Sinha doi:10.1038/nbt0813-665 French scorn Sunshine   p666 Barbara Casassus doi:10.1038/nbt0813-666a Melanoma combination therapies ward off tumor resistance   pp666 - 668 Ken Garber doi:10.1038/nbt0813-666b Dengue clinches Takeda deal   p667 Aaron Bouchie doi:10.1038/nbt0813-667 Indiana's game-changing venture   p668 Emma Dorey doi:10.1038/nbt0813-668 Paper firm to improve poor farmers' crops   p669 Anna Meldolesi doi:10.1038/nbt0813-669b Volunteer GM wheat, mischief or carelessness?   pp669 - 670 Jeffrey L Fox doi:10.1038/nbt0813-669a Around the world in a month   p670 doi:10.1038/nbt0813-670 SARS-like virus reignites ownership feuds   p671 Lucas Laursen doi:10.1038/nbt0813-671 Data Page 2Q13—an IPO revival   p672 Walter Yang doi:10.1038/nbt.2663 News Feature Spreading biotech dollars around Washington   pp673 - 675 Melanie Senior doi:10.1038/nbt.2651 The drug industry leads the US in lobbying spending. What does all that money get them? Melanie Senior investigates. Bioentrepreneur Top Building a business Stock options and beyond   pp676 - 680 John J Cannon III and Mark Kessel doi:10.1038/nbt.2662 SciCafé Startups on the Menu   p680 doi:10.1038/nbt0813-680 Opinion and Comment Top Correspondence Heritable gene targeting in the mouse and rat using a CRISPR-Cas system   pp681 - 683 Dali Li, Zhongwei Qiu, Yanjiao Shao, Yuting Chen, Yuting Guan et al. doi:10.1038/nbt.2661 Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems   pp684 - 686 Wei Li, Fei Teng, Tianda Li and Qi Zhou doi:10.1038/nbt.2652 Targeted genome modification of crop plants using a CRISPR-Cas system   pp686 - 688 Qiwei Shan, Yanpeng Wang, Jun Li, Yi Zhang, Kunling Chen et al. doi:10.1038/nbt.2650 Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9   pp688 - 691 Jian-Feng Li, Julie E Norville, John Aach, Matthew McCormack, Dandan Zhang et al. doi:10.1038/nbt.2654 Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease   pp691 - 693 Vladimir Nekrasov, Brian Staskawicz, Detlef Weigel, Jonathan D G Jones and Sophien Kamoun doi:10.1038/nbt.2655 Chinese hamster genome sequenced from sorted chromosomes OPEN   pp694 - 695 Karina Brinkrolf, Oliver Rupp, Holger Laux, Florian Kollin, Wolfgang Ernst et al. doi:10.1038/nbt.2645 Commentary The rarest of bounties   p696 Brady Huggett doi:10.1038/nbt.2649 In a land where drugs for ultra-rare indications are the new blockbusters, Alexion's Soliris is king. Features Top Public biotech 2012—the numbers   pp697 - 703 Brady Huggett doi:10.1038/nbt.2653 Initial public offerings are back. And big biotech is getting bigger: another year of profitability, increasing drug sales and bustling partnering activity. Patents The European BRCA patent oppositions and appeals: coloring inside the lines   pp704 - 710 Gert Matthijs, Isabelle Huys, Geertrui Van Overwalle and Dominique Stoppa-Lyonnet doi:10.1038/nbt.2644 The patents on BRCA1 and BRCA2 held by Myriad Genetics have been the subject of much attention in the United States recently, but the fire was lit in Europe more than a decade ago. Recent patent applications in drug discovery automation   p711 doi:10.1038/nbt.2669 News and Views Top From embryonic stem cells to mature photoreceptors   pp712 - 713 David M Gamm and Lynda S Wright doi:10.1038/nbt.2648 Photoreceptor precursors generated from embryonic stem cells integrate and mature after transplantation into the diseased mouse retina. See also: Research by Gonzalez-Cordero et al. Network cleanup   pp714 - 715 Babak Alipanahi and Brendan J Frey doi:10.1038/nbt.2657 The erroneous links in networks inferred from data can be efficiently eliminated under certain conditions. See also: Research by Barzel & Barabasi | Research by Feizi et al. Taking the fish out of fish oil   pp716 - 717 James P Wynn doi:10.1038/nbt.2656 Extensive metabolic engineering of the yeast Yarrowia lipolytica results in a sustainable and commercially viable alternative to fish oil for the supply of eicosapentaenoic acid. See also: Research by Xue et al. A caffeine fix for human nuclear transfer?   pp717 - 719 Anthony C F Perry doi:10.1038/nbt.2658 Efficient human nuclear-transfer embryo cloning is here at last, prompting renewed efforts to explore hidden variables in the cloning process. Research Highlights   p719 doi:10.1038/nbt.2659 Biotechnology JOBS of the week Head of Immune Biology (M / F) Roche Diagnostics GmbH Post Doc I Cold Spring Harbor Laboratory Member of academic staff / Postdoc (E 13 TV-L) TU Dresden Postdoctoral fellow / Junior Researcher St. Anne´s University Hospital Brno, International Clinical Research Center PhD student position in metabolic and process engineering Chalmers University of Technology More Science jobs from Biotechnology EVENT Applications for Synthetic Biology in Industrial Biotechnology November 29, 2013 London, UK More science events from Research Top Analysis Network link prediction by global silencing of indirect correlations   pp720 - 725 Baruch Barzel and Albert-Laszlo Barabasi doi:10.1038/nbt.2601 By mathematically 'silencing' spurious, indirect correlations in networks, two groups devise approaches for improving many different types of network analyses. See also: News and Views by Alipanahi & Frey Network deconvolution as a general method to distinguish direct dependencies in networks   pp726 - 733 Soheil Feizi, Daniel Marbach, Muriel Medard and Manolis Kellis doi:10.1038/nbt.2635 By mathematically 'silencing' spurious, indirect correlations in networks, two groups devise approaches for improving many different types of network analyses. See also: News and Views by Alipanahi & Frey Article Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica    pp734 - 740 Zhixiong Xue, Pamela L Sharpe, Seung-Pyo Hong, Narendra S Yadav, Dongming Xie et al. doi:10.1038/nbt.2622 Metabolic engineering of the oleaginous yeast Yarrowia lipolytica greatly enhances yields of omega-3 eicosapentaenoic acid. Letters Photoreceptor precursors derived from three-dimensional embryonic stem cell cultures integrate and mature within adult degenerate retina   pp741 - 747 Anai Gonzalez-Cordero, Emma L West, Rachael A Pearson, Yanai Duran, Livia S Carvalho et al. doi:10.1038/nbt.2643 Optic cup-like structures generated from mouse ES cells produce photoreceptor precursors that integrate and mature in vivo in three models of retinal degeneration. See also: News and Views by Gamm & Wright Single-cell gene expression analysis reveals genetic associations masked in whole-tissue experiments   pp748 - 752 Quin F Wills, Kenneth J Livak, Alex J Tipping, Tariq Enver, Andrew J Goldson et al. doi:10.1038/nbt.2642 New phenotypes of single-nucleotide polymorphisms are revealed by analyzing single cells from different individuals rather than bulk cell samples. Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies   pp753 - 758 Christoph Spiess, Mark Merchant, Arthur Huang, Zhong Zheng, Nai-Ying Yang et al. doi:10.1038/nbt.2621 In a bacterial expression system, two half antibodies, each having distinct light chains, spontaneously assemble into an intact bispecific molecule with natural antibody architecture Resources Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome OPEN   pp759 - 765 Nathan E Lewis, Xin Liu, Yuxiang Li, Harish Nagarajan, George Yerganian et al. doi:10.1038/nbt.2624 Genome sequences of the Chinese hamster and six Chinese hamster ovary cell lines provide a resource to aid in improving production of recombinant proteins. Careers and Recruitment Top Second-quarter biotech job picture   p766 Michael Francisco doi:10.1038/nbt.2660 People People   p768 doi:10.1038/nbt.2664 Top Advertisement nature.com webcasts Macmillan Science Communication presents a custom webcast on:  Accelerating the discovery and development of monoclonal antibodies  Listen on demand, to this free webcast Sponsored by:   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

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