Nature Methods Application Notes e-UPDATE: 11 June 2013

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11 JUNE 2013  Application Notes bring you the latest information about innovative tools and technologies and their applications in the lab. We hope that you will find this service useful and informative and encourage you to sign up for future Updates to ensure that you never miss one!

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FEATURED APPLICATION NOTE Mosquito® www.ttplabtech.com > A simple and automated solution for miniaturising reaction volumes for Nextera NGS sample preparation. PDF

		Rapid, directional RNA-seq library preparation kits for formalin-fixed paraffin-embedded RNA www.epibio.com > Preparing RNA-seq libraries using RNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue is notoriously difficult, due to limited sample amounts and RNA degradation. Here we describe the ScriptSeq™ Complete Kits, which enable researchers to go from as little as 100 ng of total FFPE RNA to directional, cluster-ready RNA-seq libraries in 1 d. The libraries are virtually free of contaminating ribosomal RNA (rRNA) and exhibit high quality scores and percentage of mapped reads. PDF

		Faster, cleaner bisulfite conversion that yields more intact DNA for downstream analysis www.promega.com > Performing DNA purification, bisulfite conversion and amplification are crucial steps in DNA methylation analysis, especially when working with difficult sample types like formalin-fixed paraffin-embedded (FFPE) tissue. The MethylEdge™ Bisulfite Conversion System provides a rapid, efficient method for bisulfite conversion with minimal DNA fragmentation in less than 2 h. Coupled with purification chemistries optimized for FFPE tissue and flexible, robust amplification technologies for detection, the MethylEdge™ Bisulfite Conversion System delivers high-quality converted DNA for downstream analysis. PDF

		Peggy™: size- or charge-based western blotting at the push of a button www.proteinsimple.com > http://www.sbhdiagnostics.com > Western blotting continues to be the most popular method used for protein analysis, in spite of its drawbacks in quantitation and reproducibility and its laborious workflow. Protein Simple's platform of Simple Western™ products provides a new standard in instrumentation that fully automates the archaic western blotting process and provides highly reproducible and quantitative data. Now Peggy™, the newest addition to the Simple Western™ family, provides immunoassays with separation by size or charge in a single instrument. PDF

		RNAzol® BD: a reagent for the effective isolation of RNA from whole blood www.mrcgene.com > Previously, we introduced an advanced version of the single-step method for RNA extraction. We have now adapted this methodology for the isolation of total RNA, mRNA, small RNA and microRNA from whole blood, plasma or serum of human and animal origin. This new reagent, RNAzol® BD, provides the highest yield and purity of blood-derived RNA, and it also allows for the simultaneous isolation of DNA from samples used for RNA isolation. PDF

		Effect of microRNA GC Content on RNA Purification Efficiency www.norgenbiotek.com > Unlike messenger RNA and large RNA species, a slight change in base composition in microRNA (miRNA) will drastically alter the molecules GC content and its binding preference to targets. It has been commonly assumed that standard RNA isolation methods work equally well for all miRNAs without bias. In this study, we have shown that GC content does indeed bias purification efficiency in most phenol-based methods tested, and that the best non-biased isolation product is the Silicon Carbide-based technology from Norgen Biotek. PDF

		A Comparison of Commercially-Available Human Skeletal Muscle Cells and Media for Research Applications www.cookmyosite.com > Human primary cells play a critical role in academic and industry research alike. However, isolating cells in-house is difficult and may lead to inconsistent isolations, and purchasing cells from a commercial source presents the challenge of finding the most appropriate and reliable source. Cook MyoSite, Inc. has leveraged years of controlled manufacturing experience to develop the Skeletal Muscle Derived Cells (SkMDC) product which provides superior viability, purity, and characterization compared to other commercially available sources. PDF

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