Nature Biotechnology Contents: Volume 31 pp 1 - 84

Advertisement Academy of Military Medical Sciences - Celebrating 60 years of research at one of China's leading organizations for medical science. The Academy of Military Medical Sciences (AMMS) in Beijing has developed beyond its military heritage to become a world leader in medical science with a string of achievements in both military and civilian applications that have resonated around the globe. Find out more about the AMMS in Part 5 of the five-part series of this special sponsor feature on nature.com TABLE OF CONTENTS January 2013 Volume 31, Issue 1 Editorial News Bioentrepreneur Opinion and Comment Features News and Views Computational Biology Research Careers and Recruitment Subscribe   Facebook   RSS   Recommend to library   Twitter   Advertisement BIOPHARMA DEALMAKERS Company Profiles and Partnering Opportunities   Advertisement Do you want to save money on your lab costs? Receive exclusive discounts from top suppliers of lab products and keep up-to-date with the latest product information as published in Nature and Nature Methods. Nature.com marketplace is the new product focused website brought to you by Nature Publishing Group. Visit www.nature.com/marketplace   Editorial Top Failure to launch   p1 doi:10.1038/nbt.2482 A slew of disappointing product launches suggests biotech companies are ill prepared to navigate an increasingly parsimonious reimbursement environment. News Top Pfizer's first-in-class JAK inhibitor pricey for rheumatoid arthritis market   pp3 - 4 Ken Garber doi:10.1038/nbt0113-3 MEK inhibitor nears approval   p4 Malorye Allison doi:10.1038/nbt0113-4 Cardiac stem cell therapies inch toward clinical litmus test   pp5 - 6 Cormac Sheridan doi:10.1038/nbt0113-5 IOM smacks down California Institute of Regenerative Medicine   p7 Laura DeFrancesco doi:10.1038/nbt0113-7 Anthrax drug first antibacterial mAb to win approval   p8 Jeffrey L Fox doi:10.1038/nbt0113-8 India flouts patent for blockbuster biologic   p9 Killugudi Jayaraman doi:10.1038/nbt0113-9 Kite and NCI partner on T cells   p10 Moheb Costandi doi:10.1038/nbt0113-10a Pan-African genomics   p10 Linda Nordling doi:10.1038/nbt0113-10b Threat to global GM soybean access as patent nears expiry   pp10 - 11 Daniel Grushkin doi:10.1038/nbt0113-10c Banking iPS cells   p11 Nuala Moran doi:10.1038/nbt0113-11 Algal biofuels questioned   p12 Emily Waltz doi:10.1038/nbt0113-12a Around the world in a month   p12 doi:10.1038/nbt0113-12b Bioentrepreneur Top Building a business There and back again   pp13 - 15 John Boyle doi:10.1038/nbt.2466 Opinion and Comment Top Correspondence The challenge of personal genomics in Germany   pp16 - 17 Effy Vayena and Barbara Prainsack doi:10.1038/nbt.2469 The International Serious Adverse Events Consortium's data sharing model   pp17 - 19 Jorge L Contreras, Aris Floratos and Arthur L Holden doi:10.1038/nbt.2470 Lack of evidence for existence of noncanonical RNA editing   pp19 - 20 Robert Piskol, Zhiyu Peng, Jun Wang and Jin Billy Li doi:10.1038/nbt.2472 Human embryonic stem cells commonly display large mitochondrial DNA deletions   pp20 - 23 Lindsey Van Haute, Claudia Spits, Mieke Geens, Sara Seneca and Karen Sermon doi:10.1038/nbt.2473 Knockout mice created by TALEN-mediated gene targeting   pp23 - 24 Young Hoon Sung, In-Jeoung Baek, Duk Hyoung Kim, Jisun Jeon, Jaehoon Lee, Kyunghee Lee, Daewon Jeong, Jin-Soo Kim and Han-Woong Lee doi:10.1038/nbt.2477 Features Top Patents The evolving landscape of plant varietal rights in the United States, 1930-2008   pp25 - 29 Philip Pardey, Bonwoo Koo, Jennifer Drew, Jeffrey Horwich and Carol Nottenburg doi:10.1038/nbt.2467 The types of plants being protected, by whom and by what form of varietal right, has changed markedly since the United States first enabled intellectual property protection for plant varieties in 1930. Recent patent applications in computational biotechnologies   p30 Julien Muzard doi:10.1038/nbt.2483 News and Views Top Reprogramming paces the heart   pp31 - 32 Edward G Lakatta and Victor A Maltsev doi:10.1038/nbt.2480 Rodent cardiomyocytes are converted into pacemaker cells by viral delivery of a single transcription-factor gene. See also: Research by Kapoor et al. Double or nothing on cancer immunotherapy   pp33 - 34 Ken-ichi Hanada and Nicholas P Restifo doi:10.1038/nbt.2471 Engineered T cells expressing two receptors distinguish malignant cells from healthy cells even in the absence of a tumor-specific antigen. See also: Research by Kloss et al. Automating the construction of gene ontologies   pp34 - 35 Kara Dolinski and David Botstein doi:10.1038/nbt.2476 Manual curation of biological ontologies is recapitulated by an algorithmic approach, supplementing the Gene Ontology and enabling the discovery of relationships among genes and proteins. See also: Computational Biology by Dutkowski et al. Restoration of the gut microbial habitat as a disease therapy   pp35 - 37 David A Relman doi:10.1038/nbt.2475 An intestinal infectious disease that is difficult to treat is cured by a defined set of bacterial species. RESEARCH HIGHLIGHTS   p37 doi:10.1038/nbt.2479 Biotechnology JOBS of the week Chair, Senior Lectureship / Research Fellowship and Lectureship Levels - Department of Molecular Biology & Biotechnology University of Sheffield Director of Institute for Bioscience and Biotechnology Research University of Maryland and National Institute of Standards and Technology Director of Institute for Bioscience and Biotechnology Research-IBBR Position #118732 University of Maryland and National Institute of Standards and Technology University of Maryland and National Institute of Standards and Technology Director of Research Genesis Biotechnology Group More Science jobs from Biotechnology EVENT 2nd Biotechnology World Congress February 18-21, 2013 Dubai, UAE More science events from Computational Biology Top Analysis A gene ontology inferred from molecular networks   pp38 - 45 Janusz Dutkowski, Michael Kramer, Michal A Surma, Rama Balakrishnan, J Michael Cherry, Nevan J Krogan and Trey Ideker doi:10.1038/nbt.2463 High-throughput network maps are used to automatically (or semi-automatically) reconstruct an ontology that recapitulates much of the Gene Ontology and finds additional terms and relations. See also: News and Views by Dolinski & Botstein Research Top Articles Differential analysis of gene regulation at transcript resolution with RNA-seq   pp46 - 53 Cole Trapnell, David G Hendrickson, Martin Sauvageau, Loyal Goff, John L Rinn and Lior Pachter doi:10.1038/nbt.2450 The Cuffdiff 2 algorithm improves analysis of RNA-Seq data by accounting for sample-to-sample biological variability and the complexity of transcript isoforms. Direct conversion of quiescent cardiomyocytes to pacemaker cells by expression of Tbx18    pp54 - 62 Nidhi Kapoor, Wenbin Liang, Eduardo Marban and Hee Cheol Cho doi:10.1038/nbt.2465 Rodent cardiomyocytes are converted to sinoatrial-node pacemaker cells by expression of the transcription factor Tbx18. See also: News and Views by Lakatta & Maltsev Mass-encoded synthetic biomarkers for multiplexed urinary monitoring of disease   pp63 - 70 Gabriel A Kwong, Geoffrey von Maltzahn, Gayathree Murugappan, Omar Abudayyeh, Steven Mo, Ioannis A Papayannopoulos, Deanna Y Sverdlov, Susan B Liu, Andrew D Warren, Yury Popov, Detlef Schuppan and Sangeeta N Bhatia doi:10.1038/nbt.2464 Kwong et al. use nanoparticles coated with protease substrates to generate mass-encoded synthetic biomarkers for sensitive detection of fibrosis and cancer in mice. Letter Combinatorial antigen recognition with balanced signaling promotes selective tumor eradication by engineered T cells   pp71 - 75 Christopher C Kloss, Maud Condomines, Marc Cartellieri, Michael Bachmann and Michel Sadelain doi:10.1038/nbt.2459 To increase the tumor specificity of engineered T cells, Kloss et al. design an approach that relies on T cell recognition of two, rather than one, antigens. See also: News and Views by Hanada & Restifo Resources A ligation-independent cloning technique for high-throughput assembly of transcription activator-like effector genes   pp76 - 81 Jonathan L Schmid-Burgk, Tobias Schmidt, Vera Kaiser, Klara Honing and Veit Hornung doi:10.1038/nbt.2460 A library of DNA constructs enables high-throughput, ligation-free production of transcription activator-like effector (TALE) genes for genetic engineering. Careers and Recruitment Top Rising compensation for biotech R&D officers   p82 Michael Francisco doi:10.1038/nbt.2474 People People   p84 doi:10.1038/nbt.2485 Advertisement New Partnering Opportunities in Alzheimer's Disease! A comprehensive interactive dashboard from Relay Technology Management. Click here for more information on Alzheimer's partnering opportunities.   Natureevents is a fully searchable, multi-disciplinary database designed to maximise exposure for events organisers. The contents of the Natureevents Directory are now live. The digital version is available here. Find the latest scientific conferences, courses, meetings and symposia on natureevents.com. For event advertising opportunities across the Nature Publishing Group portfolio please contact natureevents@nature.com More Nature Events

You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/myaccount (You will need to log in to be recognised as a nature.com registrant) For further technical assistance, please contact our registration department For print subscription enquiries, please contact our subscription department For other enquiries, please contact our customer feedback department Nature Publishing Group | 75 Varick Street, 9th Floor | New York | NY 10013-1917 | USA Nature Publishing Group's worldwide offices: London - Paris - Munich - New Delhi - Tokyo - Melbourne San Diego - San Francisco - Washington - New York - Boston Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at Brunel Road, Houndmills, Basingstoke, Hampshire RG21 6XS. © 2013 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.