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| 13 NOVEMBER 2012 Application Notes bring you the latest information about innovative tools and technologies and their applications in the lab. We hope that you will find this service useful and informative and encourage you to sign up for future Updates to ensure that you never miss one! | ||||||||||
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 | | Rapid and Non-Phenol Extraction of Circulating and Exosomal RNA from Bodily Fluids www.norgenbiotek.com > Norgen Biotek has developed various products (Plasma/Serum Circulating RNA Purification Kits and Exosome RNA Purification Kits) that are specifically optimized for purifying circulating or exosomal RNA (large RNA and microRNA) from plasma/serum or urine with the following advantages: Simple and Fast Procedure with no lengthy phenol:chloroform extraction, filtration or precipitation Scalable Purification of RNA from 50 µL to 5 mL of plasma/serum and 1 mL to 10 mL of urine High Yield, High Quality and Inhibitor-Free RNA isolated from all types of plasma, serum or urine
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 | | High-content analysis of biomarker intensity and distribution in 3D microtissues www.perkinelmer.com > Three-dimensional (3D) cell culture methods are widely accepted as more physiologically relevant than conventional 2D cell culture methods and are believed to improve the prediction of drug candidates at an early stage of the drug development process. Here we describe the analysis of a spherical colon cancer microtissue model using the Operetta® High Content Imaging System. In vivo near-infrared (NIR) agents allowed visualization and quantification of cancer-associated biomarker intensity and distribution in microtissues.
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 | | Long-span, mate-pair scaffolding and other methods for faster next-generation sequencing library creation www.lucigen.com > The NxSeq™ 40 kb Mate-Pair Cloning Kit facilitates the creation of scaffolds for de novo genome assembly. Supporting either Illumina or 454 sequencing, the kit produces long-span, mate-pair sequences with greater efficiency than existing protocols. In addition, NxSeq DNA Sample Prep Kits can be used to streamline workflow and speed up DNA library preparation for next-generation sequencing.
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 | | Understanding the impact of cancer cells' metabolic phenotype and intervention strategies www.seahorsebio.com > Cancer cells exhibit high rates of glycolysis, even in the presence of oxygen, a phenomenon known as the Warburg effect. While normal cells generate most of their ATP via oxidative phosphorylation, cancer cells are more reliant on glycolysis to generate ATP. Energy production through glycolysis results in a net production of protons; as glycolysis occurs, protons are extruded into the extracellular environment, acidifying the medium surrounding the cells or tissue.
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 | | Qualitative and Quantitative Characterization of the Metabolome, Lipidome, and Proteome of Human Hepatocytes www.waters.com > A label-free multi-omics approach has been applied for the analysis of the transfected human hepatocyte cells by implementing LC/HDMS[E], providing both qualitative and quantitative information within a single experiment.
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 | | ProDM™ – A kit for tryptic digestion monitoring for a successful shotgun proteomics www.itsibio.com > Successful shotgun proteomics depends on optimal digestion of proteins into peptides prior to LC/MS/MS. Current methods to determine the status of protein digestion are time consuming and/or require expensive reagents and equipment. We describe a method for determining the extent of protein digestion using ProDM™ kit and a basic spectrophotometer. Determining whether the protein is digested or not prior to LC/MS/MS will avoid wasting instrument time, samples and money.
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