Laboratory Investigation - Table of Contents alert Volume 92 Issue 10

TABLE OF CONTENTS Volume 92, Issue 10 (October 2012) In this issue Inside LI Research Articles Also new AOP Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement Commemorating Breast Cancer Awareness month with leading breast cancer research from Laboratory Investigation and Modern Pathology Enjoy free access to eight breakthrough articles, chosen by our editors, for the months of September and October.   Inside LI Top Inside Lab Invest 2012 92: 1388-1389; 10.1038/labinvest.2012.130 Full Text Research Articles Top BREAST, SKIN, SOFT TISSUE AND BONE In situ measurement of miR-205 in malignant melanoma tissue supports its role as a tumor suppressor microRNA Quantitative assessment of miRNAs in situ will allow rapid, accurate measurement of miRNAs in large cohorts of patients sampled in tissue microarrays. This approach is used successfully to assess the prognostic value of tumor suppressor miR-205 in melanoma and suggests potential for the use of miRNAs in future prognostic or predictive models. Jason A Hanna, Lewis Hahn, Seema Agarwal and David L Rimm 2012 92: 1390-1397; advance online publication, August 13, 2012; 10.1038/labinvest.2012.119 Abstract | Full Text VEGF, FLT3 ligand, PlGF and HGF can substitute for M-CSF to induce human osteoclast formation: implications for giant cell tumour pathobiology Increased osteoclast formation and activity is seen in giant cell tumor of bone (GCTB), which contains numerous large hyper-nucleated osteoclastic giant cells. Growth factors present in GCTB can induce human osteoclast formation and may explain the cytomorphology of GCTB giant cells and the osteolytic behavior of this tumor Richard M Taylor, Takeshi G Kashima, Helen J Knowles and Nicholas A Athanasou 2012 92: 1398-1406; advance online publication, August 20, 2012; 10.1038/labinvest.2012.108 Abstract | Full Text GASTROINTESTINAL, HEPATIC AND PANCREATIC SYSTEMS MDR1-P-glycoprotein behaves as an oncofetal protein that promotes cell survival in gastric cancer cells P-glycoprotein behaves as an anti-apoptotic agent and confers a strong survival advantage to gastric cancer cells by cross-talk with Bcl-xL. The functional significance of P-glycoprotein in the early phases of Helicobacter pylori-related gastric carcinogenesis could make it a useful biomarker for selection of patients at major risk of progressive gastric disease. Alba Rocco, Debora Compare, Eleonora Liguori, Alessandra Cianflone, Giuseppe Pirozzi, Virginia Tirino, Alessandra Bertoni, Margherita Santoriello, Corrado Garbi, Maria D'Armiento, Stefania Staibano and Gerardo Nardone 2012 92: 1407-1418; advance online publication, July 2, 2012; 10.1038/labinvest.2012.100 Abstract | Full Text Bradykinin inhibits hepatic gluconeogenesis in obese mice The vasodilator bradykinin and its B2 receptor are now shown to modulate glucose homeostasis by controlling Foxo1-mediated hepatic glucose production. This observation points to a protective role of the kallikrein-kinin system in the pathophysiology of type 2 diabetes. Carlos Castilho Barros, Anderson Haro, Fernanda Jaqueline Russo, Ines Schadock, Sandro Soares Almeida, Felipe Castellani Reis, Milton Rocha Moraes, Andre Haidar, Aparecida Emiko Hirata, Marcelo Mori, Reury Frank Pereira Bacurau, Martin Würtele, Michael Bader, Joao Bosco Pesquero and Ronaldo Carvalho Araujo 2012 92: 1419-1427; advance online publication, August 6, 2012; 10.1038/labinvest.2012.105 Abstract | Full Text Increased intrahepatic resistance in severe steatosis: endothelial dysfunction, vasoconstrictor overproduction and altered microvascular architecture The assessment of intrahepatic resistance and its determinants shows that severe steatosis induces a haemodynamically significant increase in intrahepatic resistance that precedes inflammation and fibrogenesis. Both functional (endothelial dysfunction and increased thromboxane and ET-1 synthesis) and structural factors are involved. This phenomenon may contribute significantly to steatosis-related disease. Sven Francque, Wim Laleman, Len Verbeke, Christophe Van Steenkiste, Christophe Casteleyn, Wilhelmus Kwanten, Christophe Van Dyck, Michiel D'Hondt, Albert Ramon, Wim Vermeulen, Benedicte De Winter, Eric Van Marck, Veerle Van Marck, Paul Pelckmans and Peter Michielsen 2012 92: 1428-1439; advance online publication, August 13, 2012; 10.1038/labinvest.2012.103 Abstract | Full Text Atorvastatin inhibits proliferation and apoptosis, but induces senescence in hepatic myofibroblasts and thereby attenuates hepatic fibrosis in rats Atorvastatin prevents liver fibrosis and is also a beneficial treatment for established fibrosis. The mechanisms are twofold: the prevention of hepatic stellate cell (HSC) activation, and decreased cytokine and collagen production in previously activated HSCs. Furthermore, atorvastatin decreases turnover and induces senescence of activated HSCs and hepatic myofibroblasts in injured livers. Sabine Klein, Jeremias Klösel, Robert Schierwagen, Christian Körner, Michaela Granzow, Sebastian Huss, Irela Gretchen Reza Mazar, Susanne Weber, Peter F M van den Ven, Ursula Pieper-Fürst, Dieter O Fürst, Jacob Nattermann, Frank Lammert, Tilman Sauerbruch and Jonel Trebicka 2012 92: 1440-1450; advance online publication, August 13, 2012; 10.1038/labinvest.2012.106 Abstract | Full Text Monoamine oxidase A expression is suppressed in human cholangiocarcinoma via coordinated epigenetic and IL-6-driven events The expression of monoamine oxidase A (MAOA) is suppressed in cholangiocarcinoma. The mechanism involves hypermethylation of the MAOA promoter region and the interleukin-6-driven balance between SP-1 transcriptional activity and its inhibitor, R1 repressor. Restoration of MAOA expression inhibits cholangiocarcinoma growth and invasion. Li Huang, Gabriel Frampton, Arundhati Rao, Kun-song Zhang, Wei Chen, Jia-ming Lai, Xiao-yu Yin, Kimberly Walker, Brianne Culbreath, Dinorah Leyva-Illades, Matthew Quinn, Matthew McMillin, Michelle Bradley, Li-Jian Liang and Sharon DeMorrow 2012 92: 1451-1460; advance online publication, August 20, 2012; 10.1038/labinvest.2012.110 Abstract | Full Text BLOOD, LYMPHATICS, IMMUNE SYSTEM AND STEM CELLS Liver inflammation in a mouse model of Th1 hepatitis despite the absence of invariant NKT cells or the Th1 chemokine receptors CXCR3 and CCR5 In autoimmune hepatitis and viral hepatitis, liver damage is dependent on infiltration by CD4+ T cells, which can accumulate independently of either of the traditional chemokine receptors responsible for Th1-cell movement into sites of inflammation. CD4+ T cells are therefore capable of significant liver accumulation and subsequent damage through additional unidentified pathways. James G Cripps, Stela Celaj, Marie Burdick, Robert M Strieter and James D Gorham 2012 92: 1461-1471; advance online publication, August 20, 2012; 10.1038/labinvest.2012.104 Abstract | Full Text Evidence questioning cromolyn’s effectiveness and selectivity as a ‘mast cell stabilizer’ in mice The so-called “mast cell stabilizer”, cromolyn, has been used in mice to investigate the roles of mast cells in vivo. However, tests of mast cells in vitro, and in normal or mast cell-deficient mice in vivo, question cromolyn's effectiveness and selectivity as an inhibitor of mast cell function in mice. Tatsuya Oka, Janet Kalesnikoff, Philipp Starkl, Mindy Tsai and Stephen J Galli 2012 92: 1472-1482; advance online publication, August 20, 2012; 10.1038/labinvest.2012.116 Abstract | Full Text MODELS AND TECHNIQUES A canine autosomal recessive model of collagen type III glomerulopathy The first animal model of collagen type III glomerulopathy with massive glomerular collagen type III deposition is described. Afflicted animals exhibit mesangial collagen type III synthesis, and genetic studies exclude mutations in the canine Col3A1 gene. This model may be useful for study of mechanisms of excess collagen accumulation. Runa Rørtveit, Frode Lingaas, Tina Bønsdorff, Anna V Eggertsdóttir, Ann M Grøndahl, Ragnar Thomassen, Agnes B Fogo and Johan H Jansen 2012 92: 1483-1491; advance online publication, August 13, 2012; 10.1038/labinvest.2012.112 Abstract | Full Text Multicolored stain-free histopathology with coherent Raman imaging Conventional histopathology with hematoxylin & eosin has been the gold standard for histopathology. An alternative multicolored method to visualize tissue in real-time using coherent Raman imaging has been developed. The histopathological images show resolution similar to conventional techniques but do not require tissue fixation, sectioning or staining of the tissue analyzed. Christian W Freudiger, Rolf Pfannl, Daniel A Orringer, Brian G Saar, Minbiao Ji, Qing Zeng, Linda Ottoboni, Wei Ying, Christian Waeber, John R Sims, Philip L De Jager, Oren Sagher, Martin A Philbert, Xiaoyin Xu, Santosh Kesari, X Sunney Xie and Geoffrey S Young 2012 92: 1492-1502; advance online publication, August 20, 2012; 10.1038/labinvest.2012.109 Abstract | Full Text Dissecting the phenotypes of Plk1 inhibition in cancer cells using novel kinase inhibitory chemical CBB2001 Polo-like kinase 1 (Plk1) is a serine/threonine kinase that has a critical role in mitosis. Plk1 expression is associated with cell proliferation and is overexpressed in a variety of cancers. A novel kinase inhibitory chemical, CBB2001, is described that specifically inhibits Plk1 kinase activity, indicating its potential as an anti-cancer agent. Rongfeng Lan, Guimiao Lin, Feng Yin, Jun Xu, Xiaoming Zhang, Jing Wang, Yanchao Wang, Jianxian Gong, Yuan-Hua Ding, Zhen Yang, Fei Lu and Hui Zhang 2012 92: 1503-1514; advance online publication, August 13, 2012; 10.1038/labinvest.2012.114 Abstract | Full Text Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department. For print subscription enquiries, please contact our subscription department. For other enquiries, please contact our customer feedback department. 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