The EDGE

EuroFlow: Resetting leukemia and lymphoma immunophenotyping. Basis for companion diagnostics and personalized medicine

J J M van Dongen and A Orfao on behalf of the EuroFlow Consortium

Leukemia 2012 26: 1899-1907; 10.1038/leu.2012.121

EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes

J J M van Dongen, L Lhermitte, S Böttcher, J Almeida, V H J van der Velden, J Flores-Montero, A Rawstron, V Asnafi, Q Lécrevisse, P Lucio, E Mejstrikova, T Szczepański, T Kalina, R de Tute, M Brüggemann, L Sedek, M Cullen, A W Langerak, A Mendonça, E Macintyre, M Martin-Ayuso, O Hrusak, M B Vidriales and A Orfao on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)

Leukemia 2012 26: 1908-1975; advance online publication, May 3, 2012; 10.1038/leu.2012.120

Flow cytometric immunobead assay for fast and easy detection of PML–RARA fusion proteins for the diagnosis of acute promyelocytic leukemia

E H A Dekking, V H J van der Velden, R Varro, H Wai, S Böttcher, M Kneba, E Sonneveld, A Koning, N Boeckx, N Van Poecke, P Lucio, A Mendonça, L Sedek, T Szczepański, T Kalina, V Kanderová, P Hoogeveen, J Flores-Montero, M C Chillón, A Orfao, J Almeida, P Evans, M Cullen, A L Noordijk, P M Vermeulen, M T de Man, E P Dixon, W M Comans-Bitter and J J M van Dongen on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)

Leukemia 2012 26: 1976-1985; advance online publication, May 8, 2012; 10.1038/leu.2012.125

EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols

T Kalina, J Flores-Montero, V H J van der Velden, M Martin-Ayuso, S Böttcher, M Ritgen, J Almeida, L Lhermitte, V Asnafi, A Mendonça, R de Tute, M Cullen, L Sedek, M B Vidriales, J J Pérez, J G te Marvelde, E Mejstrikova, O Hrusak, T Szczepański, J J M van Dongen and A Orfao on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)

Leukemia 2012 26: 1986-2010; 10.1038/leu.2012.122

Spotlight Review

MiR-125 in normal and malignant hematopoiesis

L Shaham, V Binder, N Gefen, A Borkhardt and S Izraeli

Leukemia 2012 26: 2011-2018; advance online publication, March 29, 2012; 10.1038/leu.2012.90

Review

Natural killer cell immune escape in acute myeloid leukemia

E Lion, Y Willemen, Z N Berneman, V F I Van Tendeloo and E L J Smits

Leukemia 2012 26: 2019-2026; advance online publication, March 26, 2012; 10.1038/leu.2012.87

Concise Review

Spliceosome and other novel mutations in chronic lymphocytic leukemia and myeloid malignancies

F Damm, F Nguyen-Khac, M Fontenay and O A Bernard

Leukemia 2012 26: 2027-2031; advance online publication, April 9, 2012; 10.1038/leu.2012.86

Design of novel BH3 mimetics for the treatment of chronic lymphocytic leukemia

C Billard

Leukemia 2012 26: 2032-2038; advance online publication, March 28, 2012; 10.1038/leu.2012.88

Original Articles

MOLECULAR TARGETS FOR THERAPY

Lysine-specific demethylase 1 restricts hematopoietic progenitor proliferation and is essential for terminal differentiation

A Sprüssel, J H Schulte, S Weber, M Necke, K Händschke, T Thor, K W Pajtler, A Schramm, K König, L Diehl, P Mestdagh, J Vandesompele, F Speleman, H Jastrow, L C Heukamp, R Schüle, U Dührsen, R Buettner, A Eggert and J R Göthert

Leukemia 2012 26: 2039-2051; advance online publication, June 13, 2012; 10.1038/leu.2012.157

ACUTE LEUKEMIAS

Specific small nucleolar RNA expression profiles in acute leukemia

W Valleron, E Laprevotte, E-F Gautier, C Quelen, C Demur, E Delabesse, X Agirre, F Prósper, T Kiss and P Brousset

Leukemia 2012 26: 2052-2060; advance online publication, April 23, 2012; 10.1038/leu.2012.111

Phase IB study of the FLT3 kinase inhibitor midostaurin with chemotherapy in younger newly diagnosed adult patients with acute myeloid leukemia

R M Stone, T Fischer, R Paquette, G Schiller, C A Schiffer, G Ehninger, J Cortes, H M Kantarjian, D J DeAngelo, A Huntsman-Labed, C Dutreix, A del Corral and F Giles

Leukemia 2012 26: 2061-2068; advance online publication, April 27, 2012; 10.1038/leu.2012.115

Notch signaling expands a pre-malignant pool of T-cell acute lymphoblastic leukemia clones without affecting leukemia-propagating cell frequency

J S Blackburn, S Liu, D M Raiser, S A Martinez, H Feng, N D Meeker, J Gentry, D Neuberg, A T Look, S Ramaswamy, A Bernards, N S Trede and D M Langenau

Leukemia 2012 26: 2069-2078; advance online publication, April 27, 2012; 10.1038/leu.2012.116

Pretransplant HLA mistyping in diagnostic samples of acute myeloid leukemia patients due to acquired uniparental disomy

V Dubois, F Sloan-Béna, A Cesbron, B G Hepkema, K Gagne, S Gimelli, D Heim, A Tichelli, J Delaunay, M Drouet, S Jendly, J Villard and J-M Tiercy

Leukemia 2012 26: 2079-2085; advance online publication, March 14, 2012; 10.1038/leu.2012.68

Real-time PCR quantification of major Wilms’ tumor gene 1 (WT1) isoforms in acute myeloid leukemia, their characteristic expression patterns and possible functional consequences

K Kramarzova, J Stuchly, A Willasch, B Gruhn, J Schwarz, J Cermak, K Machova-Polakova, O Fuchs, J Stary, J Trka and L Boublikova

Leukemia 2012 26: 2086-2095; advance online publication, March 20, 2012; 10.1038/leu.2012.76

CHRONIC MYELOPROLIFERATIVE NEOPLASIAS

Early molecular and cytogenetic response is predictive for long-term progression-free and overall survival in chronic myeloid leukemia (CML)

B Hanfstein, M C Müller, R Hehlmann, P Erben, M Lauseker, A Fabarius, S Schnittger, C Haferlach, G Göhring, U Proetel, H-J Kolb, S W Krause, W-K Hofmann, J Schubert, H Einsele, J Dengler, M Hänel, C Falge, L Kanz, A Neubauer, M Kneba, F Stegelmann, M Pfreundschuh, C F Waller, S Branford, T P Hughes, K Spiekermann, G M Baerlocher, M Pfirrmann, J Hasford, S Saußele and A Hochhaus for the SAKK and the German CML Study Group

Leukemia 2012 26: 2096-2102; advance online publication, March 26, 2012; 10.1038/leu.2012.85

LYMPHOMA

Comprehensive gene expression profiling and immunohistochemical studies support application of immunophenotypic algorithm for molecular subtype classification in diffuse large B-cell lymphoma: a report from the International DLBCL Rituximab-CHOP Consortium Program Study

C Visco, Y Li, Z Y Xu-Monette, R N Miranda, T M Green, Y Li, A Tzankov, W Wen, W-m Liu, B S Kahl, E S G d'Amore, S Montes-Moreno, K Dybkær, A Chiu, W Tam, A Orazi, Y Zu, G Bhagat, J N Winter, H-Y Wang, S O'Neill, C H Dunphy, E D Hsi, X F Zhao, R S Go, W W L Choi, F Zhou, M Czader, J Tong, X Zhao, J H van Krieken, Q Huang, W Ai, J Etzell, M Ponzoni, A J M Ferreri, M A Piris, M B Møller, C E Bueso-Ramos, L J Medeiros, L Wu and K H Young

Leukemia 2012 26: 2103-2113; advance online publication, March 22, 2012; 10.1038/leu.2012.83

MYELOMA

Constitutive activation of p38 MAPK in tumor cells contributes to osteolytic bone lesions in multiple myeloma

J Yang, J He, J Wang, Y Cao, J Ling, J Qian, Y Lu, H Li, Y Zheng, Y Lan, S Hong, J Matthews, M W Starbuck, N M Navone, R Z Orlowski, P Lin, L W Kwak and Q Yi

Leukemia 2012 26: 2114-2123; advance online publication, March 19, 2012; 10.1038/leu.2012.71

Small molecule antibody targeting HLA class I inhibits myeloma cancer stem cells by repressing pluripotency-associated transcription factors

A Ikegame, S Ozaki, D Tsuji, T Harada, S Fujii, S Nakamura, H Miki, A Nakano, K Kagawa, K Takeuchi, M Abe, K Watanabe, M Hiasa, N Kimura, Y Kikuchi, A Sakamoto, K Habu, M Endo, K Itoh, H Yamada-Okabe and T Matsumoto

Leukemia 2012 26: 2124-2134; advance online publication, March 20, 2012; 10.1038/leu.2012.78

CD138-negative clonogenic cells are plasma cells but not B cells in some multiple myeloma patients

N Hosen, Y Matsuoka, S Kishida, J Nakata, Y Mizutani, K Hasegawa, A Mugitani, H Ichihara, Y Aoyama, S Nishida, A Tsuboi, F Fujiki, N Tatsumi, H Nakajima, M Hino, T Kimura, K Yata, M Abe, Y Oka, Y Oji, A Kumanogoh and H Sugiyama

Leukemia 2012 26: 2135-2141; advance online publication, March 20, 2012; 10.1038/leu.2012.80

Letters to the Editor

Retention of CD34⁺ CML stem/progenitor cells during imatinib treatment and rapid decline after treatment with second-generation BCR–ABL inhibitors

Y Minami, A Abe, M Minami, K Kitamura, J Hiraga, S Mizuno, K Ymamoto, M Sawa, Y Inagaki, K Miyamura and T Naoe

Leukemia 2012 26: 2142-2143; advance online publication, March 19, 2012; 10.1038/leu.2012.73

Newly described activating JAK3 mutations in T-cell acute lymphoblastic leukemia

T Bains, M C Heinrich, M M Loriaux, C Beadling, D Nelson, A Warrick, T L Neff, J W Tyner, J Dunlap, C L Corless and G Fan

Leukemia 2012 26: 2144-2146; advance online publication, March 19, 2012; 10.1038/leu.2012.74

Increased CD200 expression in acute myeloid leukemia is linked with an increased frequency of FoxP3⁺ regulatory T cells

S J Coles, R K Hills, E C Y Wang, A K Burnett, S Man, R L Darley and A Tonks

Leukemia 2012 26: 2146-2148; advance online publication, March 20, 2012; 10.1038/leu.2012.75

Expression of CD200 on AML blasts directly suppresses memory T-cell function

S J Coles, R K Hills, E C Y Wang, A K Burnett, S Man, R L Darley and A Tonks

Leukemia 2012 26: 2148-2151; advance online publication, March 20, 2012; 10.1038/leu.2012.77

Elucidation of a novel pathogenomic mechanism using genome-wide long mate-pair sequencing of a congenital t(16;21) in a series of three RUNX1-mutated FPD/AML pedigrees

A Buijs, M Poot, S van der Crabben, B van der Zwaag, E van Binsbergen, M J van Roosmalen, M Tavakoli-Yaraki, O de Weerdt, H K Nieuwenhuis, M van Gijn and W P Kloosterman

Leukemia 2012 26: 2151-2154; advance online publication, March 20, 2012; 10.1038/leu.2012.79

Effects of L-leucine in 5q- syndrome and other RPS14-deficient erythroblasts

B H Yip, A Pellagatti, C Vuppusetty, A Giagounidis, U Germing, A A Lamikanra, D J Roberts, M Fernandez-Mercado, E-J McDonald, S Killick, J S Wainscoat and J Boultwood

Leukemia 2012 26: 2154-2158; advance online publication, March 20, 2012; 10.1038/leu.2012.82