Laboratory Investigation - Table of Contents alert Volume 92 Issue 9

TABLE OF CONTENTS Volume 92, Issue 9 (September 2012) In this issue Inside LI Research Articles Also new AOP This alert is sponsored by Be among the first to discover Hamamatsu's new NanoZoomer whole-slide scanner. This soon-to-be-released instrument offers new features that guarantee blur-free, error-free, and hassle-free scanning of whole slides. Our latest addition to the NanoZoomer product line will continue to serve the imaging community with the robust performance and excellent image quality you've come to expect from Hamamatsu. Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement Recommended by the Editors of LI: MicroRNAs that target Ca2+ transporters are involved in vascular smooth muscle cell calcification   Inside LI Top Inside Lab Invest 2011 92: 1248-1249; 10.1038/labinvest.2012.120 Full Text Research Articles Top ANGIOGENESIS, CARDIOVASCULAR AND PULMONARY SYSTEMS MicroRNAs that target Ca2+ transporters are involved in vascular smooth muscle cell calcification Hyperphosphatemia and hypercalcemia induce vascular smooth muscle cell (VSMC) calcification and are associated with increased cardiovascular mortality. Four specific miRNA molecules are potential therapeutic targets in vascular calcification. These miRNAs disrupt Ca2+ efflux proteins in VSMC, thereby increasing intracellular Ca2+ concentration, resulting in vascular calcification. Ting Gui, Gengyin Zhou, Yujing Sun, Aiko Shimokado, Shunji Itoh, Kosuke Oikawa and Yasuteru Muragaki 2012 92: 1250-1259; advance online publication, June 11, 2012; 10.1038/labinvest.2012.85 Abstract | Full Text Functional defects of CD46-induced regulatory T cells to suppress airway inflammation in mite allergic asthma Loss of regulatory T (Treg) cell function is critical to allergic asthma pathogenesis. Activated Tregs from asthma exhibit decreased IL-10/granzyme B, CCR4 expression and chemotactic signaling pathway, which may explain the inability to suppress airway epithelial cell inflammation. Additionally, manipulation of Treg recruitment may help attain tolerance and suppress airway inflammation. Yi-Giien Tsai, Dau-Ming Niu, Kuender D Yang, Chih-Hsing Hung, Ya-Ju Yeh, Chia-Ying Lee and Ching-Yuang Lin 2012 92: 1260-1269; advance online publication, July 2, 2012; 10.1038/labinvest.2012.86 Abstract | Full Text Myofibroblasts in interstitial lung diseases show diverse electron microscopic and invasive features Myofibroblasts may have diverse molecular compositions and distinct ultrastructural features in different types of lung disease. Cells obtained from eight serious lung disorders were cultured from bronchoalveolar lavage (BAL) fluid samples. Characterization of the cells revealed several differential features which could be useful in diagnostics and future therapeutics. Henna M Karvonen, Siri T Lehtonen, Raija T Sormunen, Terttu H Harju, Elisa Lappi-Blanco, Risto S Bloigu and Riitta L Kaarteenaho 2012 92: 1270-1284; advance online publication, June 18, 2012; 10.1038/labinvest.2012.95 Abstract | Full Text Protective effects of long pentraxin PTX3 on lung injury in a severe acute respiratory syndrome model in mice Long pentraxin PTX3 is a humoral mediator of innate immunity that has clinically important anti-viral effects. Administration of recombinant PTX3 to a mouse model of severe acute respiratory syndrome accelerates viral clearance in the lung, attenuates virus-induced lung injury, and reduces early neutrophil influx and elevation of inflammatory mediators. Bing Han, Xuezhong Ma, Jianhua Zhang, Yu Zhang, Xiaohui Bai, David M Hwang, Shaf Keshavjee, Gary A Levy, Ian McGilvray and Mingyao Liu 2012 92: 1285-1296; advance online publication, June 25, 2012; 10.1038/labinvest.2012.92 Abstract | Full Text Transforming growth factor-beta1 induces microvascular abnormalities through a down-modulation of neural cell adhesion molecule in human hepatocellular carcinoma Conventional chemotherapy can be ineffective for hepatocarcinoma (HCC) because of the abnormal structural organization of tumor-infiltrating vessels. The role of TGF-β1 and neural cell adhesion molecule (NCAM) in HCC-induced abnormal vascular morphogenesis are described and provide the rationale for treatment of HCC patients with chemotherapy and TGF-β1 inhibitors. Piera Balzarini, Anna Benetti, Gloria Invernici, Silvia Cristini, Sonia Zicari, Arnaldo Caruso, Luisa B Gatta, Angiola Berenzi, Luisa Imberti, Cinzia Zanotti, Nazario Portolani, Stefano M Giulini, Maura Ferrari, Emilio Ciusani, Stefania E Navone, Alessandra Canazza, Eugenio A Parati and Giulio Alessandri 2012 92: 1297-1309; advance online publication, June 25, 2012; 10.1038/labinvest.2012.94 Abstract | Full Text BREAST, SKIN, SOFT TISSUE AND BONE Micro-RNA-632 downregulates DNAJB6 in breast cancer DNAJB6, a member of the HSP40 family, is a negative regulator of breast tumor progression. miR-632 targets the coding region of DNAJB6, resulting in the down regulation of DNAJB6 expression. miR-632 therefore plays a supportive role in malignant progression and is a potential treatment target in breast cancer. Aparna Mitra, Jack W Rostas, Donna L Dyess, Lalita A Shevde and Rajeev S Samant 2012 92: 1310-1317; advance online publication, June 18, 2012; 10.1038/labinvest.2012.87 Abstract | Full Text The suppression of TRIM21 and the accumulation of IFN-α play crucial roles in the pathogenesis of osteonecrosis of the femoral head Osteonecrosis of the femoral head (ONFH) leads to degenerative arthritis of the hip joint in autoimmune disease patients. TRIM21 autoantibodies inhibit the E3 ligase activity of TRIM21, causing a downstream accumulation of IFN-α, cell death, and development of ONFH. TRIM21 and IFN-α may therefore be targets for preventive manipulation of ONFH. Kenji Tateda, Shunichiro Okazaki, Satoshi Nagoya, Ryuichi Katada, Keisuke Mizuo, Satoshi Watanabe, Toshihiko Yamashita and Hiroshi Matsumoto 2012 92: 1318-1329; advance online publication, July 23, 2012; 10.1038/labinvest.2012.89 Abstract | Full Text Distinctive contact between CD34+ hematopoietic progenitors and CXCL12+ CD271+ mesenchymal stromal cells in benign and myelodysplastic bone marrow Mesenchymal stromal cells (MSCs) support hematopoiesis but are abnormal in myelodysplastic syndrome (MDS), implying a pathophysiologic role in MDS and utility as a diagnostic tool. This study quantitatively and comprehensively maps the normal MSC component of intact bone marrow to allow identification of derangements of MSCs in myeloid neoplasia. Eugenia Flores-Figueroa, Sushama Varma, Kelli Montgomery, Peter L Greenberg and Dita Gratzinger 2012 92: 1330-1341; advance online publication, June 18, 2012; 10.1038/labinvest.2012.93 Abstract | Full Text MODELS AND TECHNIQUES Evaluating tumor heterogeneity in immunohistochemistry-stained breast cancer tissue Quantitative clinical measurement of heterogeneity in immunohistochemistry staining may be useful in evaluating patient therapeutic response and for histopathology laboratory quality control. Using HER2 immunohistochemistry stained breast cancer samples, a heterogeneity scoring approach was developed that allows the visualization of an individual patient's immunohistochemistry heterogeneity in the context of a patient population. Steven J Potts, Joseph S Krueger, Nicholas D Landis, David A Eberhard, G David Young, Steven C Schmechel and Holger Lange 2012 92: 1342-1357; advance online publication, July 16, 2012; 10.1038/labinvest.2012.91 Abstract | Full Text Infrared spectral histopathology (SHP): a novel diagnostic tool for the accurate classification of lung cancer Spectral diagnostic methods allow reproducible and objective diagnosis of unstained tissue sections. A comparison between classical and spectral histopathology for the classification of lung cancer histopathological samples from a tissue microarray suggests that spectral histopathology can achieve levels of diagnostic accuracy that is comparable to that of multi-panel immunohistochemistry. Benjamin Bird, Milo Miljković, Stan Remiszewski, Ali Akalin, Mark Kon and Max Diem 2012 92: 1358-1373; advance online publication, July 2, 2012; 10.1038/labinvest.2012.101 Abstract | Full Text Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis Pseudopodia play a role in cell migration and pseudopodia proteomics may enhance our understanding of malignant phenotypes of tumor cells. A pseudopodia proteomics methodology was developed using an excimer laser and two-dimensional difference gel electrophoresis with ultrahigh sensitive fluorescent dye. Pseudopodia-enriched proteins were identified, and one such protein, RAB1A, is directly involved in pseudopodia formation. Akihiko Ito, Takahiro Mimae, Ying-Shan-Zhu Yamamoto, Man Hagiyama, Jun Nakanishi, Masaoki Ito, Yoichiroh Hosokawa, Morihito Okada, Yoshinori Murakami and Tadashi Kondo 2012 92: 1374-1385; advance online publication, July 2, 2012; 10.1038/labinvest.2012.98 Abstract | Full Text Advertisement Recommended by the Editors of Modern Pathology: The European Consensus on grading of bone marrow fibrosis allows a better prognostication of patients with primary myelofibrosis   Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. You can change or discontinue your e-mail alerts at any time, by modifying your preferences on your nature.com account at: www.nature.com/nams/svc/myaccount (You will need to log in to be recognised as a nature.com registrant). For further technical assistance, please contact our registration department. For print subscription enquiries, please contact our subscription department. For other enquiries, please contact our customer feedback department. Nature Publishing Group | 75 Varick Street, 9th Floor | New York | NY 10013-1917 | USA Nature Publishing Group's worldwide offices: London - Paris - Munich - New Delhi - Tokyo - Melbourne San Diego - San Francisco - Washington - New York - Boston Macmillan Publishers Limited is a company incorporated in England and Wales under company number 785998 and whose registered office is located at Brunel Road, Houndmills, Basingstoke, Hampshire RG21 6XS. © 2012 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.