Laboratory Investigation - Table of Contents alert Volume 92 Issue 4

TABLE OF CONTENTS Volume 92, Issue 4 (April 2012) In this issue Inside LI Research Articles Also new AOP Sign up for e-alerts Recommend to your library Web feed Subscribe Advertisement April editor's recommendation from LI: SSeCKS sequesters cyclin D1 in glomerular parietal epithelial cells and influences proliferative injury in the glomerulus Glomerular parietal epithelial cells (PECs) are progenitors for podocytes, but the mechanisms that allow for repeated periods of cell-cycle arrest and re-entry of PECs after glomerulogenesis are unknown. The multivalent scaffolding PKC substrate, SSeCKS, regulates the activity of cyclin D1 in PECs, serving as a gating checkpoint for cell-cycle arrest of PECs but not podocytes.   Inside LI Top Inside Lab Invest 2012 92: 486-487; 10.1038/labinvest.2012.51 Full Text Research Articles Top GENITOURINARY AND REPRODUCTIVE SYSTEMS Indoxyl sulfate-induced epithelial-to-mesenchymal transition and apoptosis of renal tubular cells as novel mechanisms of progression of renal disease Indoxyl sulfate accumulates in patients with chronic kidney disease and is a marker of renal dysfunction, but whether or not it plays an active role in the pathology is unclear. Indoxyl sulfate-induced renal damage is now shown to involve epithelial-to-mesenchymal transition and MAP kinase-induced apoptosis of renal tubular cells. Su Hyun Kim, Min-A Yu, Eun Sun Ryu, Yang-Hee Jang and Duk-Hee Kang 2012 92: 488-498; advance online publication, January 9, 2012; 10.1038/labinvest.2011.194 Abstract | Full Text SSeCKS sequesters cyclin D1 in glomerular parietal epithelial cells and influences proliferative injury in the glomerulus Glomerular parietal epithelial cells (PECs) are progenitors for podocytes but the mechanisms that allow for repeated periods of cell-cycle arrest and re-entry of PECs after glomerulogenesis are unknown. The multivalent scaffolding PKC substrate, SSeCKS, regulates the activity of cyclin D1 in PECs, serving as a gating checkpoint for cell-cycle arrest of PECs but not podocytes. Bettina Burnworth, Jeff Pippin, Prasanthi Karna, Shin Akakura, Ron Krofft, Guoqiang Zhang, Kelly Hudkins, Charles E Alpers, Kelly Smith, Stuart J Shankland, Irwin H Gelman and Peter J Nelson 2012 92: 499-510; advance online publication, January 16, 2012; 10.1038/labinvest.2011.199 Abstract | Full Text Sox4 functions as a positive regulator of β-catenin signaling through upregulation of TCF4 during morular differentiation of endometrial carcinomas In endometrial carcinoma, focal squamous differentiation into a morular phenotype is linked with increased probability of survival. Sox4 is a positive regulator of β-catenin signaling, altering expression of the transcription factor TCF4 during morular differentiation, leading to inhibition of proliferation. Makoto Saegusa, Miki Hashimura and Takeshi Kuwata 2012 92: 511-521; advance online publication, January 9, 2012; 10.1038/labinvest.2011.196 Abstract | Full Text PERIPHERAL AND CENTRAL NERVOUS SYSTEM Transplantation of telencephalic neural progenitors induced from embryonic stem cells into subacute phase of focal cerebral ischemia A new method for generation and isolation of neural progenitors from embryonic stem cells is described. When the cells are transplanted into ischemic mouse putamen after transient middle cerebral artery occlusion, neuronal network formation and functional recovery from ischemic damage is observed. Motoaki Fujimoto, Hideki Hayashi, Yasushi Takagi, Makoto Hayase, Takeshi Marumo, Masanori Gomi, Masaki Nishimura, Hiroharu Kataoka, Jun Takahashi, Nobuo Hashimoto, Kazuhiko Nozaki and Susumu Miyamoto 2012 92: 522-531; advance online publication, February 13, 2012; 10.1038/labinvest.2012.1 Abstract | Full Text Estradiol triggers sonic-hedgehog-induced angiogenesis during peripheral nerve regeneration by downregulating hedgehog-interacting protein Local injections of estradiol before nerve injury improve functional recovery and vascular growth afterward by downregulating hedgehog-interacting protein and promoting the hedgehog signaling pathway. These observations contribute to our understanding of the mechanisms of nerve repair and may have important implications for the treatment of traumatic nerve injury. Haruki Sekiguchi, Masaaki Ii, Kentaro Jujo, Marie-Ange Renault, Tina Thorne, Trevor Clarke, Aiko Ito, Toshikazu Tanaka, Ekaterina Klyachko, Yasuhiko Tabata, Nobuhisa Hagiwara and Douglas Losordo 2012 92: 532-542; advance online publication, February 13, 2012; 10.1038/labinvest.2012.6 Abstract | Full Text ENDOCRINE, VISUAL AND AUDITORY SYSTEMS Inhibition of Gsk3β activity improves β-cell function in c-KitWv/+ male mice Reduction of the PI3K/Akt signaling pathway contributes to β-cell dysfunction in c-KitWv/+ mice, while inhibition of Gsk3β prevents the onset of diabetes and maintains β-cell mass and function, demonstrating a functional role for c-Kit in mediating glucose homeostasis and β-cell function via the Akt/Gsk3β axis. Zhi-Chao Feng, Lisa Donnelly, Jinming Li, Mansa Krishnamurthy, Matthew Riopel and Rennian Wang 2012 92: 543-555; advance online publication, January 16, 2012; 10.1038/labinvest.2011.200 Abstract | Full Text Interleukin-1 receptor mediates the interplay between CD4+ T cells and ocular resident cells to promote keratinizing squamous metaplasia in Sjögren's syndrome Chronic inflammation in autoimmune diseases such as Sjögren's syndrome can lead to blinding keratinizing squamous metaplasia of the ocular surface. A central role for IL-1 is revealed in the pathogenesis of this disease; it orchestrates a functional interplay between autoreactive CD4+ T cells and resident cells of the ocular surface. Ying-Ting Chen, Stanislav Lazarev, Ahmad F Bahrami, Lisa B Noble, Feeling Y T Chen, Delu Zhou, Marianne Gallup, Mahesh Yadav and Nancy A McNamara 2012 92: 556-570; advance online publication, January 9, 2012; 10.1038/labinvest.2011.189 Abstract | Full Text BREAST, SKIN, SOFT TISSUE AND BONE Downregulation of microRNAs miR-1, -206 and -29 stabilizes PAX3 and CCND2 expression in rhabdomyosarcoma The transcription factor PAX3 is aberrantly expressed in rhabdomyosarcoma (RMS). The tumor suppressors miR-1 and -206 are downregulated in RMS, resulting in stabilized PAX3 expression. In alveolar RMS, PAX3 loses its 3'UTR and evades miRNA regulation. RMS is further stabilized by deregulation of miR-29 and its target cell cycle genes. Lihua Li, Aaron L Sarver, Setara Alamgir and Subbaya Subramanian 2012 92: 571-583; advance online publication, February 13, 2012; 10.1038/labinvest.2012.10 Abstract | Full Text Application of a partial-thickness human ex vivo skin culture model in cutaneous wound healing study A unique human ex vivo skin culture model for use in research of wound repair and skin diseases is described. It has several advantages over traditional 3-D organotypic cultures for the study of the dermal-epidermal junction, superficial wound closure, and regeneration of collagen post-wounding. Wei Xu, Seok Jong Hong, Shengxian Jia, Yanan Zhao, Robert D Galiano and Thomas A Mustoe 2012 92: 584-599; advance online publication, January 9, 2012; 10.1038/labinvest.2011.184 Abstract | Full Text CD14− mononuclear stromal cells support (CD14+) monocyte–osteoclast differentiation in aneurysmal bone cyst Bone destruction in aneurysmal bone cysts (ABC) is due to giant cells, which express an osteoclast-like phenotype and are formed from RANKL-expressing CD14+ macrophage precursors by interaction with CD14− mononuclear stromal cells. The molecular mechanisms of ABC osteolysis suggest that pharmacological inhibitors of osteoclasts are a potential treatment for ABC. Richard MI Taylor, Takeshi G Kashima, Francesca K E Hemingway, Arundhati Dongre, Helen J Knowles and Nicholas A Athanasou 2012 92: 600-605; advance online publication, February 13, 2012; 10.1038/labinvest.2012.5 Abstract | Full Text BLOOD, LYMPHATICS, IMMUNE SYSTEM AND STEM CELLS Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells Cancer-initiating cells (CICs) generally maintain reactive oxygen species (ROS) at low levels and, high aldehyde dehydrogenase (ALDH) activity and are in a quiescent state of the cell cycle. However, in Hodgkin lymphoma, the cell cycle status among ALDH-high and ROS-low cells varies, suggesting heterogeneity of CIC-like populations in this disease. Jun-ichiro Ikeda, Suhana Mamat, Tian Tian, Yi Wang, Wenjuan Luo, Nur Rahadiani, Katsuyuki Aozasa and Eiichi Morii 2012 92: 606-614; advance online publication, February 20, 2012; 10.1038/labinvest.2012.4 Abstract | Full Text Sjögren's syndrome autoantibodies provoke changes in gene expression profiles of inflammatory cytokines triggering a pathway involving TACE/NF-κB The inflammatory gene expression profile observed in the chronic inflammatory autoimmune disorder Sjögren's syndrome is associated with changes in TNF-α converting enzyme, TNF-α and NF-κB levels. Pathways that include TNF-α signaling converge on NF-κB and contribute to the pathology of this condition. Sabrina Lisi, Margherita Sisto, Dario Domenico Lofrumento and Massimo D'Amore 2012 92: 615-624; advance online publication, December 12, 2011; 10.1038/labinvest.2011.190 Abstract | Full Text ORAL AND GASTROINTESTINAL SYSTEMS Gluten induces coeliac-like disease in sensitised mice involving IgA, CD71 and transglutaminase 2 interactions that are prevented by probiotics Gluten-sensitive BALB/c mice represent a new animal model of gluten- induced enteropathy that closely resembles human celiac disease. In these animals, lesions are associated with IgA, CD71 and transglutaminase-2 interactions and local pro-inflammatory cytokine production. Probiotics improve this enteropathy, and suggest a new therapeutic approach in humans Christina Papista, Vassilis Gerakopoulos, Andreas Kourelis, Maria Sounidaki, Anastasia Kontana, Laureline Berthelot, Ivan C Moura, Renato C Monteiro and Minas Yiangou 2012 92: 625-635; advance online publication, February 13, 2012; 10.1038/labinvest.2012.13 Abstract | Full Text TECHNICAL REPORT Assaying macrophage activity in a murine model of inflammatory bowel disease using fluorine-19 MRI Perfluorocarbon imaging agents and 19F magnetic resonance imaging can be used to quantify macrophage burden longitudinally. These methods can be used to assess the topology and severity of macrophages in inflammatory bowel disease and are effective for evaluating early-stage inflammation and therapeutics. Deepak K Kadayakkara, Sarangarajan Ranganathan, Won-Bin Young and Eric T Ahrens 2012 92: 636-645; advance online publication, February 13, 2012; 10.1038/labinvest.2012.7 Abstract | Full Text Advertisement Laboratory Investigation Web Focus: Renal Pathology Kidney disease affects over 26 million Americans, resulting in a high demand for elucidating the pathways that lead to renal pathologies and identifying potential therapeutic targets. In this web focus presented by Laboratory Investigation, recent research in the field of renal pathology is highlighted, FREE for a limited time. Visit http://www.nature.com/labinvest/webfocus/index.html to access the full listing of articles in this special web focus FREE for a limited time.   Please note that you need to be a subscriber or site-licence holder to enjoy full-text access to Laboratory Investigation. In order to do so, please purchase a subscription. You have been sent this Table of Contents Alert because you have opted in to receive it. 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